Restriction map for
pFN21ASDA1133
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AviII BalI
BcgI BfrI BlpI Bpu1102I BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI MfeI MluI MluNI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 1405 GTAGAC GT^[AC][GT]AC
AccIII 2379 TCCGGA T^CCGGA
AcsI 444 GAATTC [GA]^AATT[CT]
AcyI 518 GGCGCC G[GA]^CG[CT]C
AgeI 638 ACCGGT A^CCGGT
AlwNI 2082 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1788 CCTCAGG CC^T[ACGT]AGG
ApoI 444 GAATTC [GA]^AATT[CT]
AspI 434 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 117 CCTAGG C^CTAGG
BamHI 311 GGATCC G^GATCC
BanI 518 GGCGCC G^G[CT][GA]CC
BbeI 518 GGCGCC GGCGC^C
BbsI 217 GAAGAC GAAGAC(2/6)
BciVI 2283 GGATAC GTATCC(6/5)
BclI 195 TGATCA T^GATCA
BglII 2473 AGATCT A^GATCT
BlnI 117 CCTAGG C^CTAGG
BpuAI 217 GAAGAC GAAGAC(2/6)
BsaBI 273 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 518 GGCGCC G[GA]^CG[CT]C
BseAI 2379 TCCGGA T^CCGGA
BsiEI 807 CGGCCG CG[GA][CT]^CG
BspEI 2379 TCCGGA T^CCGGA
BssSI 1813 CACGAG CACGAG(-5/-1)
BstAPI 2081 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 783 GGTGACC G^GT[ACGT]ACC
BstPI 783 GGTGACC G^GT[ACGT]ACC
BstXI 581 CCATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1788 CCTCAGG CC^T[ACGT]AGG
EagI 807 CGGCCG C^GGCCG
EclXI 807 CGGCCG C^GGCCG
Eco52I 807 CGGCCG C^GGCCG
Eco81I 1788 CCTCAGG CC^T[ACGT]AGG
EcoO65I 783 GGTGACC G^GT[ACGT]ACC
EcoRI 444 GAATTC G^AATTC
FbaI 195 TGATCA T^GATCA
Hin1I 518 GGCGCC G[GA]^CG[CT]C
HincII 283 GTCAAC GT[CT]^[GA]AC
HindII 283 GTCAAC GT[CT]^[GA]AC
KasI 518 GGCGCC G^GCGCC
KspI 2064 CCGCGG CCGC^GG
MamI 273 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2379 TCCGGA T^CCGGA
MseI 266 TTAA T^TAA
NarI 518 GGCGCC GG^CGCC
NcoI 893 CCATGG C^CATGG
PflFI 434 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 975 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 638 ACCGGT A^CCGGT
SacI 1594 GAGCTC GAGCT^C
SacII 2064 CCGCGG CCGC^GG
SapI 1221 GCTCTTC GCTCTTC(1/4)
SfoI 518 GGCGCC GGC^GCC
Tru9I 266 TTAA T^TAA
Tth111I 434 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 975 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 474 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbrPI 764 CACGTG CAC^GTG
1240 CACGTG
BbvCI 1128 CCTCAGC CCTCAGC(-5/-2)
1467 CCTCAGC
BglI 1519 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2452 GCCACTGAGGC
BsaAI 764 CACGTG [CT]AC^GT[GA]
1240 CACGTG
BsaWI 638 ACCGGT [AT]^CCGG[AT]
2379 TCCGGA
BstYI 311 GGATCC [GA]^GATC[CT]
2473 AGATCT
HaeII 518 GGCGCC [GA]GCGC^[CT]
1870 AGCGCC
HgaI 434 GACGC GACGC(5/10)
2271 GACGC
MflI 311 GGATCC [GA]^GATC[CT]
2473 AGATCT
MslI 1230 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1609 CACAGTCATG
PaeR7I 302 CTCGAG C^TCGAG
1759 CTCGAG
PmaCI 764 CACGTG CAC^GTG
1240 CACGTG
PmlI 764 CACGTG CAC^GTG
1240 CACGTG
SfaNI 259 GATGC GCATC(5/9)
2183 GCATC
Tsp509I 89 AATT ^AATT
445 AATT
XhoI 302 CTCGAG C^TCGAG
1759 CTCGAG
XhoII 311 GGATCC [GA]^GATC[CT]
2473 AGATCT
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI ApaI ApaLI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI SmaI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XmaCI XmaI