(Circular) (Six-base) MAPSORT of: pFN21ASDA1133.seq Check: 7059 from: 1 to: 7237 pFN21ASDA1133 7237 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2717 3710 4069 2717 Size: 993 359 5885 Fragments arranged by size: 5885 993 359 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5047 278 Size: 53 83 186 936 3511 2468 Fragments arranged by size: 3511 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3429 4580 1937 Size: 1492 1151 4594 Fragments arranged by size: 4594 1492 1151 Acc65I G'GTAC_C Cuts at: 4558 4558 Size: 7237 AclI AA'CG_TT Cuts at: 1563 5364 5737 1563 Size: 3801 373 3063 Fragments arranged by size: 3801 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2259 2468 3292 5300 6684 6952 1632 Size: 627 209 824 2008 1384 268 1917 Fragments arranged by size: 2008 1917 1384 824 627 268 209 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7040 AflIII A'CryG_T Cuts at: 1203 6136 7113 1203 Size: 4933 977 1327 Fragments arranged by size: 4933 1327 977 AgeI A'CCGG_T Cuts at: 2661 6068 2661 Size: 3407 3830 AhdI GACnn_n'nnGTC Cuts at: 5966 5966 Size: 7237 AleI CACnn'nnGTG Cuts at: 1166 3257 1166 Size: 2091 5146 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7237 AlwNI CAG_nnn'CTG Cuts at: 4110 4980 6552 4110 Size: 870 1572 4795 Fragments arranged by size: 4795 1572 870 ApaI G_GGCC'C Cuts at: 2566 3206 3671 3756 3933 4189 4381 2566 Size: 640 465 85 177 256 192 5422 Fragments arranged by size: 5422 640 465 256 192 177 85 ApaLI G'TGCA_C Cuts at: 2837 3260 3629 5294 6450 2837 Size: 423 369 1665 1156 3624 Fragments arranged by size: 3624 1665 1156 423 369 ApoI r'AATT_y Cuts at: 1493 2467 4546 4772 4924 7033 1493 Size: 974 2079 226 152 2109 1697 Fragments arranged by size: 2109 2079 1697 974 226 152 AseI AT'TA_AT Cuts at: 160 1032 5791 160 Size: 872 4759 1606 Fragments arranged by size: 4759 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7237 AvaI C'yCGr_G Cuts at: 1870 1943 2325 3198 3559 3568 3611 3682 3782 Size: 73 382 873 361 9 43 71 100 Cuts at: 3782 4181 4188 4341 4522 4562 1870 Size: 399 7 153 181 40 4545 Fragments arranged by size: 4545 873 399 382 361 181 153 100 73 71 43 40 9 7 AvrII C'CTAG_G Cuts at: 2140 2140 Size: 7237 BamHI G'GATC_C Cuts at: 2334 4567 4934 2334 Size: 2233 367 4637 Fragments arranged by size: 4637 2233 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2541 4558 6013 618 Size: 325 217 627 754 2017 1455 1842 Fragments arranged by size: 2017 1842 1455 754 627 325 217 BanII G_rGCy'C Cuts at: 1391 2237 2566 3206 3533 3560 3602 3621 3671 Size: 846 329 640 327 27 42 19 50 Cuts at: 3671 3756 3783 3870 3933 3950 3972 4189 4360 Size: 85 27 87 63 17 22 217 171 Cuts at: 4360 4381 4556 1391 Size: 21 175 4072 Fragments arranged by size: 4072 846 640 329 327 217 175 171 87 85 63 50 42 27 27 22 21 19 17 BbeI G_GCGC'C Cuts at: 2545 2545 Size: 7237 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2247 928 Size: 971 348 5918 Fragments arranged by size: 5918 971 348 BbvCI CC'TCA_GC Cuts at: 3152 3491 3152 Size: 339 6898 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5428 5462 1903 Size: 34 3491 34 3678 Fragments arranged by size: 3678 3491 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4300 5129 6339 4300 Size: 829 1210 5198 Fragments arranged by size: 5198 1210 829 BclI T'GATC_A Cuts at: 1548 2218 1548 Size: 670 6567 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3548 4481 5848 Size: 107 122 71 631 605 1876 933 1367 Cuts at: 5848 136 Size: 1525 Fragments arranged by size: 1876 1525 1367 933 631 605 122 107 71 BglII A'GATC_T Cuts at: 4496 7232 4496 Size: 2736 4501 BlpI GC'TnA_GC Cuts at: 4654 4654 Size: 7237 Bme1580I G_kGCm'C Cuts at: 1790 2566 2841 3206 3264 3633 3671 3756 3933 Size: 776 275 365 58 369 38 85 177 Cuts at: 3933 4189 4381 4444 5298 6454 1790 Size: 256 192 63 854 1156 2573 Fragments arranged by size: 2573 1156 854 776 369 365 275 256 192 177 85 63 58 38 BmgBI CAC'GTC Cuts at: 1318 2592 3300 1318 Size: 1274 708 5255 Fragments arranged by size: 5255 1274 708 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2042 3106 3967 Size: 416 53 83 399 24 625 1064 861 Cuts at: 3967 4968 5926 442 Size: 1001 958 1753 Fragments arranged by size: 1753 1064 1001 958 861 625 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7237 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4144 4144 Size: 7237 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2633 3047 3305 3397 3731 3761 4430 5897 Size: 1394 414 258 92 334 30 669 1467 Cuts at: 5897 1239 Size: 2579 Fragments arranged by size: 2579 1467 1394 669 414 334 258 92 30 Bpu10I CC'TnA_GC Cuts at: 3152 3491 4196 3152 Size: 339 705 6193 Fragments arranged by size: 6193 705 339 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2234 5362 6227 6525 2234 Size: 3128 865 298 2946 Fragments arranged by size: 3128 2946 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2413 2765 3113 5900 882 Size: 617 914 352 348 2787 2219 Fragments arranged by size: 2787 2219 914 617 352 348 BsaAI yAC'GTr Cuts at: 493 1204 2789 3265 4534 5028 493 Size: 711 1585 476 1269 494 2702 Fragments arranged by size: 2702 1585 1269 711 494 476 BsaBI GATnn'nnATC Cuts at: 2300 7231 2300 Size: 4931 2306 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2542 5044 5426 6061 Size: 53 83 186 936 1009 2502 382 635 Cuts at: 6061 275 Size: 1451 Fragments arranged by size: 2502 1451 1009 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2661 4402 5670 6068 6342 6489 6900 2661 Size: 1741 1268 398 274 147 411 2998 Fragments arranged by size: 2998 1741 1268 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3161 3191 3395 3425 3520 Size: 30 165 30 1758 30 204 30 95 Cuts at: 3520 3550 3959 3989 4518 4548 1178 Size: 30 409 30 529 30 3867 Fragments arranged by size: 3867 1758 529 409 204 165 95 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2340 2648 3629 3724 4364 4421 1186 Size: 1154 308 981 95 640 57 4002 Fragments arranged by size: 4002 1154 981 640 308 95 57 BseYI C'CCAG_C Cuts at: 2079 2857 3046 3644 3870 3880 6440 6936 2079 Size: 778 189 598 226 10 2560 496 2380 Fragments arranged by size: 2560 2380 778 598 496 226 189 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2298 2510 3358 3418 4160 1163 Size: 573 562 212 848 60 742 4240 Fragments arranged by size: 4240 848 742 573 562 212 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2833 4687 5448 6476 1540 Size: 275 186 832 1854 761 1028 2301 Fragments arranged by size: 2301 1854 1028 832 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2841 3033 3264 3621 3633 3747 5298 5383 6454 Size: 192 231 357 12 114 1551 85 1071 Cuts at: 6454 2841 Size: 3624 Fragments arranged by size: 3624 1551 1071 357 231 192 114 85 12 BsmI GAATG_Cn' Cuts at: 4748 4841 4748 Size: 93 7144 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2566 2841 3033 3206 3264 3533 Size: 399 447 329 275 192 173 58 269 Cuts at: 3533 3560 3602 3621 3633 3671 3747 3756 3783 Size: 27 42 19 12 38 76 9 27 Cuts at: 3783 3870 3933 3950 3972 4189 4360 4381 4444 Size: 87 63 17 22 217 171 21 63 Cuts at: 4444 4556 5298 5383 6454 1391 Size: 112 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 329 275 269 217 192 173 171 112 87 85 76 63 63 58 42 38 27 27 22 21 19 17 12 9 BspEI T'CCGG_A Cuts at: 4402 6900 4402 Size: 2498 4739 BspHI T'CATG_A Cuts at: 5126 6856 5126 Size: 1730 5507 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2717 3589 3710 4069 4592 844 Size: 1873 872 121 359 523 3489 Fragments arranged by size: 3489 1873 872 523 359 121 BsrBI CCG'CTC Cuts at: 1394 1823 2248 3011 3228 3291 3330 4354 5124 Size: 429 425 763 217 63 39 1024 770 Cuts at: 5124 1394 Size: 3507 Fragments arranged by size: 3507 1024 770 763 429 425 217 63 39 BsrDI GCAATG_nn' Cuts at: 65 1248 5732 5906 65 Size: 1183 4484 174 1396 Fragments arranged by size: 4484 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2257 2661 2815 2922 5881 6068 1700 Size: 110 447 404 154 107 2959 187 2869 Fragments arranged by size: 2959 2869 447 404 187 154 110 107 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7237 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7237 BssSI C'ACGA_G Cuts at: 3836 5297 6309 3836 Size: 1461 1012 4764 Fragments arranged by size: 4764 1461 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4110 794 Size: 3316 3921 BstBI TT'CG_AA Cuts at: 2007 6041 2007 Size: 4034 3203 BstEII G'GTnAC_C Cuts at: 1184 2806 1184 Size: 1622 5615 BstXI CCAn_nnnn'nTGG Cuts at: 2611 2611 Size: 7237 BstYI r'GATC_y Cuts at: 1972 2334 4496 4567 4934 5319 5336 6788 6874 Size: 362 2162 71 367 385 17 1452 86 Cuts at: 6874 6886 7232 1972 Size: 12 346 1977 Fragments arranged by size: 2162 1977 1452 385 367 362 346 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 3812 3812 Size: 7237 BtgI C'CryG_G Cuts at: 513 1065 2890 2916 3255 4087 4313 513 Size: 552 1825 26 339 832 226 3437 Fragments arranged by size: 3437 1825 832 552 339 226 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6498 Fragments arranged by size: 6498 683 56 BtsI GCAGTG_nn' Cuts at: 3238 3346 4470 4753 5547 5567 3238 Size: 108 1124 283 794 20 4908 Fragments arranged by size: 4908 1124 794 283 108 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7237 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3058 3093 529 Size: 35 2494 35 4673 Fragments arranged by size: 4673 2494 35 35 DraI TTT'AAA Cuts at: 4541 4541 Size: 7237 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6244 809 Size: 507 4928 1802 Fragments arranged by size: 4928 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2830 3006 3079 Size: 54 1425 50 275 18 1000 176 73 Cuts at: 3079 3322 3351 3394 3662 4684 5573 7202 8 Size: 243 29 43 268 1022 889 1629 43 Fragments arranged by size: 1629 1425 1022 1000 889 275 268 243 176 73 54 50 43 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2830 4684 1537 Size: 275 1018 1854 4090 Fragments arranged by size: 4090 1854 1018 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 3251 4065 4341 5167 1353 Size: 699 1199 814 276 826 3423 Fragments arranged by size: 3423 1199 826 814 699 276 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4110 5824 6198 6344 387 Size: 1064 138 2521 1714 374 146 1280 Fragments arranged by size: 2521 1714 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3619 3619 Size: 7237 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2259 2468 2633 3047 3292 3305 3397 Size: 393 627 209 165 414 245 13 92 Cuts at: 3397 3731 3761 4430 5300 5897 6684 6952 1239 Size: 334 30 669 870 597 787 268 1524 Fragments arranged by size: 1524 870 787 669 627 597 414 393 334 268 245 209 165 92 30 13 EcoNI CCTnn'n_nnAGG Cuts at: 2144 3061 3941 2144 Size: 917 880 5440 Fragments arranged by size: 5440 917 880 EcoO109I rG'GnC_Cy Cuts at: 2562 2563 3148 3202 3578 3668 3752 3819 3929 Size: 1 585 54 376 90 84 67 110 Cuts at: 3929 3930 4185 4192 4266 4327 4377 4378 2562 Size: 1 255 7 74 61 50 1 5421 Fragments arranged by size: 5421 585 376 255 110 90 84 74 67 61 54 50 7 1 1 1 EcoRI G'AATT_C Cuts at: 2467 4546 4924 2467 Size: 2079 378 4780 Fragments arranged by size: 4780 2079 378 FspI TGC'GCA Cuts at: 5743 5743 Size: 7237 HaeII r_GCGC'y Cuts at: 1678 2545 3897 6384 1678 Size: 867 1352 2487 2531 Fragments arranged by size: 2531 2487 1352 867 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2319 2351 3161 3193 3520 Size: 32 135 32 747 32 810 32 327 Cuts at: 3520 3552 3680 3712 3830 3862 3959 3991 5878 Size: 32 128 32 118 32 97 32 1887 Cuts at: 5878 5910 5952 5984 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1887 810 747 327 135 128 118 97 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 4581 4827 669 Size: 988 281 370 2273 246 3079 Fragments arranged by size: 3079 2273 988 370 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7237 HpaI GTT'AAC Cuts at: 4827 4827 Size: 7237 KasI G'GCGC_C Cuts at: 2541 2541 Size: 7237 KpnI G_GTAC'C Cuts at: 4562 4562 Size: 7237 MfeI C'AATT_G Cuts at: 4836 4836 Size: 7237 MluI A'CGCG_T Cuts at: 7113 7113 Size: 7237 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2619 6351 6535 1305 Size: 1314 3732 184 2007 Fragments arranged by size: 3732 2007 1314 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5415 Fragments arranged by size: 5415 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3257 3636 5195 5554 5713 518 Size: 648 2091 379 1559 359 159 2042 Fragments arranged by size: 2091 2042 1559 648 379 359 159 MspA1I CmG'CkG Cuts at: 1606 2167 2224 3018 3312 3905 4089 4107 4653 Size: 561 57 794 294 593 184 18 546 Cuts at: 4653 5330 6478 6723 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 794 677 593 561 546 294 245 184 57 18 NarI GG'CG_CC Cuts at: 2542 2542 Size: 7237 NcoI C'CATG_G Cuts at: 513 1065 2916 513 Size: 552 1851 4834 Fragments arranged by size: 4834 1851 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7237 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7237 NotI GC'GGCC_GC Cuts at: 4684 4684 Size: 7237 NspI r_CATG'y Cuts at: 1132 1229 4595 6140 1132 Size: 97 3366 1545 2229 Fragments arranged by size: 3366 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3988 1348 Size: 2640 4597 PciI A'CATG_T Cuts at: 6136 6136 Size: 7237 PflMI CCAn_nnn'nTGG Cuts at: 1108 3004 1108 Size: 1896 5341 PfoI T'CCnGG_A Cuts at: 4446 4446 Size: 7237 PmeI GTTT'AAAC Cuts at: 4541 4541 Size: 7237 PmlI CAC'GTG Cuts at: 2789 3265 2789 Size: 476 6761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2219 2251 5306 5338 6819 6851 2219 Size: 32 3055 32 1481 32 2605 Fragments arranged by size: 3055 2605 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3578 3819 4192 4266 3578 Size: 241 373 74 6549 Fragments arranged by size: 6549 373 241 74 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7237 PsiI TTA'TAA Cuts at: 4807 4807 Size: 7237 PspOMI G'GGCC_C Cuts at: 2562 3202 3667 3752 3929 4185 4377 2562 Size: 640 465 85 177 256 192 5422 Fragments arranged by size: 5422 640 465 256 192 177 85 PspXI vC'TCGA_Gb Cuts at: 2325 3782 2325 Size: 1457 5780 PstI C_TGCA'G Cuts at: 830 2182 2700 3707 4489 4589 830 Size: 1352 518 1007 782 100 3478 Fragments arranged by size: 3478 1352 1007 782 518 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7237 PvuII CAG'CTG Cuts at: 2224 3312 3905 2224 Size: 1088 593 5556 Fragments arranged by size: 5556 1088 593 SacI G_AGCT'C Cuts at: 3621 3621 Size: 7237 SacII CC_GC'GG Cuts at: 4090 4090 Size: 7237 SalI G'TCGA_C Cuts at: 1936 4579 1936 Size: 2643 4594 SanDI GG'GwC_CC Cuts at: 4266 4266 Size: 7237 SapI GCTCTTCn'nnn_ Cuts at: 3251 3251 Size: 7237 SbfI CC_TGCA'GG Cuts at: 4589 4589 Size: 7237 ScaI AGT'ACT Cuts at: 5485 7197 5485 Size: 1712 5525 SfcI C'TryA_G Cuts at: 826 1045 2178 2696 3703 3721 4485 4585 5720 Size: 219 1133 518 1007 18 764 100 1135 Cuts at: 5720 6401 6592 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1133 1007 764 681 518 219 191 100 18 SfoI GGC'GCC Cuts at: 2543 2543 Size: 7237 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7237 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7237 SmaI CCC'GGG Cuts at: 1872 3200 4183 4190 4343 4524 4564 1872 Size: 1328 983 7 153 181 40 4545 Fragments arranged by size: 4545 1328 983 181 153 40 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 2325 3782 5341 6242 6504 Size: 197 926 306 76 1457 1559 901 262 Cuts at: 6504 820 Size: 1553 Fragments arranged by size: 1559 1553 1457 926 901 306 262 197 76 SnaBI TAC'GTA Cuts at: 493 4534 5028 493 Size: 4041 494 2702 Fragments arranged by size: 4041 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7237 SphI G_CATG'C Cuts at: 1132 4595 1132 Size: 3463 3774 SspI AAT'ATT Cuts at: 5 52 5161 5 Size: 47 5109 2081 Fragments arranged by size: 5109 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7237 StyI C'CwwG_G Cuts at: 513 1065 2140 2412 2916 3093 3659 4261 4676 Size: 552 1075 272 504 177 566 602 415 Cuts at: 4676 513 Size: 3074 Fragments arranged by size: 3074 1075 602 566 552 504 415 272 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2043 4507 5264 5281 5434 5619 6064 Size: 1255 463 2464 757 17 153 185 445 Cuts at: 6064 325 Size: 1498 Fragments arranged by size: 2464 1498 1255 757 463 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2161 2619 2654 Size: 274 80 33 51 1443 184 458 35 Cuts at: 2654 4143 5483 7195 96 Size: 1489 1340 1712 138 Fragments arranged by size: 1712 1489 1443 1340 458 274 184 138 80 51 35 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3395 3427 7197 7229 3395 Size: 32 3770 32 3403 Fragments arranged by size: 3770 3403 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2460 1316 Size: 1144 6093 XbaI T'CTAG_A Cuts at: 2497 4573 2497 Size: 2076 5161 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2479 3421 3706 2029 Size: 450 942 285 5560 Fragments arranged by size: 5560 942 450 285 XhoI C'TCGA_G Cuts at: 1943 2325 3782 1943 Size: 382 1457 5398 Fragments arranged by size: 5398 1457 382 XmaI C'CCGG_G Cuts at: 1870 3198 4181 4188 4341 4522 4562 1870 Size: 1328 983 7 153 181 40 4545 Fragments arranged by size: 4545 1328 983 181 153 40 7 XmnI GAAnn'nnTTC Cuts at: 5366 5366 Size: 7237 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5045 276 Size: 53 83 186 936 3511 2468 Fragments arranged by size: 3511 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI RsrII SexAI SfiI SrfI SwaI