Restriction map for
pFN21ASDA1133
(Linear) (Six-base) MAPSORT of: KIAA1133.seq Check: 9216 from: 1 to: 2508
KIAA1133.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 694 1687 2046 2508
Size: 694 993 359 462
Fragments arranged by size:
993 694 462 359
AccI GT'mk_AC
Cuts at: 0 1406 2508
Size: 1406 1102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 445 1269 2508
Size: 236 209 824 1239
Fragments arranged by size:
1239 824 236 209
AgeI A'CCGG_T
Cuts at: 0 638 2508
Size: 638 1870
AleI CACnn'nnGTG
Cuts at: 0 1234 2508
Size: 1234 1274
AlwNI CAG_nnn'CTG
Cuts at: 0 2087 2508
Size: 2087 421
ApaI G_GGCC'C
Cuts at: 0 543 1183 1648 1733 1910 2166 2358 2508
Size: 543 640 465 85 177 256 192 150
Fragments arranged by size:
640 543 465 256 192 177 150 85
ApaLI G'TGCA_C
Cuts at: 0 814 1237 1606 2508
Size: 814 423 369 902
Fragments arranged by size:
902 814 423 369
ApoI r'AATT_y
Cuts at: 0 444 2508
Size: 444 2064
AvaI C'yCGr_G
Cuts at: 0 302 1175 1536 1545 1588 1659 1759 2158
Size: 302 873 361 9 43 71 100 399
Cuts at: 2158 2165 2318 2499 2508
Size: 7 153 181 9
Fragments arranged by size:
873 399 361 302 181 153 100 71
43 9 9 7
AvrII C'CTAG_G
Cuts at: 0 117 2508
Size: 117 2391
BamHI G'GATC_C
Cuts at: 0 311 2508
Size: 311 2197
BanI G'GyrC_C
Cuts at: 0 518 2508
Size: 518 1990
BanII G_rGCy'C
Cuts at: 0 214 543 1183 1510 1537 1579 1598 1648
Size: 214 329 640 327 27 42 19 50
Cuts at: 1648 1733 1760 1847 1910 1927 1949 2166 2337
Size: 85 27 87 63 17 22 217 171
Cuts at: 2337 2358 2508
Size: 21 150
Fragments arranged by size:
640 329 327 217 214 171 150 87
85 63 50 42 27 27 22 21
19 17
BbeI G_GCGC'C
Cuts at: 0 522 2508
Size: 522 1986
BbsI GAAGACnn'nnnn_
Cuts at: 0 224 2508
Size: 224 2284
BbvCI CC'TCA_GC
Cuts at: 0 1129 1468 2508
Size: 1129 339 1040
Fragments arranged by size:
1129 1040 339
BciVI GTATCCnnnnn_n'
Cuts at: 0 2277 2508
Size: 2277 231
BclI T'GATC_A
Cuts at: 0 195 2508
Size: 195 2313
BglI GCCn_nnn'nGGC
Cuts at: 0 1525 2458 2508
Size: 1525 933 50
Fragments arranged by size:
1525 933 50
BglII A'GATC_T
Cuts at: 0 2473 2508
Size: 2473 35
Bme1580I G_kGCm'C
Cuts at: 0 543 818 1183 1241 1610 1648 1733 1910
Size: 543 275 365 58 369 38 85 177
Cuts at: 1910 2166 2358 2421 2508
Size: 256 192 63 87
Fragments arranged by size:
543 369 365 275 256 192 177 87
85 63 58 38
BmgBI CAC'GTC
Cuts at: 0 569 1277 2508
Size: 569 708 1231
Fragments arranged by size:
1231 708 569
BmrI ACTGGGnnnn_n'
Cuts at: 0 19 1083 1944 2508
Size: 19 1064 861 564
Fragments arranged by size:
1064 861 564 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2121 2508
Size: 2121 387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1024 1282 1374 1708 1738 2407 2508
Size: 610 414 258 92 334 30 669 101
Fragments arranged by size:
669 610 414 334 258 101 92 30
Bpu10I CC'TnA_GC
Cuts at: 0 1129 1468 2173 2508
Size: 1129 339 705 335
Fragments arranged by size:
1129 705 339 335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 2508
Size: 211 2297
BsaI GGTCTCn'nnnn_
Cuts at: 0 390 742 1090 2508
Size: 390 352 348 1418
Fragments arranged by size:
1418 390 352 348
BsaAI yAC'GTr
Cuts at: 0 766 1242 2508
Size: 766 476 1266
Fragments arranged by size:
1266 766 476
BsaBI GATnn'nnATC
Cuts at: 0 277 2508
Size: 277 2231
BsaHI Gr'CG_yC
Cuts at: 0 519 2508
Size: 519 1989
BsaWI w'CCGG_w
Cuts at: 0 638 2379 2508
Size: 638 1741 129
Fragments arranged by size:
1741 638 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1138 1168 1372 1402 1497 1527 1936 1966
Size: 1138 30 204 30 95 30 409 30
Cuts at: 1966 2508
Size: 542
Fragments arranged by size:
1138 542 409 204 95 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 317 625 1606 1701 2341 2398 2508
Size: 317 308 981 95 640 57 110
Fragments arranged by size:
981 640 317 308 110 95 57
BseYI C'CCAG_C
Cuts at: 0 56 834 1023 1621 1847 1857 2508
Size: 56 778 189 598 226 10 651
Fragments arranged by size:
778 651 598 226 189 56 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 487 1335 1395 2137 2508
Size: 275 212 848 60 742 371
Fragments arranged by size:
848 742 371 275 212 60
BsiEI CG_ry'CG
Cuts at: 0 810 2508
Size: 810 1698
BsiHKAI G_wGCw'C
Cuts at: 0 818 1010 1241 1598 1610 1724 2508
Size: 818 192 231 357 12 114 784
Fragments arranged by size:
818 784 357 231 192 114 12
Bsp1286I G_dGCh'C
Cuts at: 0 214 543 818 1010 1183 1241 1510 1537
Size: 214 329 275 192 173 58 269 27
Cuts at: 1537 1579 1598 1610 1648 1724 1733 1760 1847
Size: 42 19 12 38 76 9 27 87
Cuts at: 1847 1910 1927 1949 2166 2337 2358 2421 2508
Size: 63 17 22 217 171 21 63 87
Fragments arranged by size:
329 275 269 217 214 192 173 171
87 87 76 63 63 58 42 38
27 27 22 21 19 17 12 9
BspEI T'CCGG_A
Cuts at: 0 2379 2508
Size: 2379 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 694 1566 1687 2046 2508
Size: 694 872 121 359 462
Fragments arranged by size:
872 694 462 359 121
BsrBI CCG'CTC
Cuts at: 0 225 988 1205 1268 1307 2331 2508
Size: 225 763 217 63 39 1024 177
Fragments arranged by size:
1024 763 225 217 177 63 39
BsrFI r'CCGG_y
Cuts at: 0 234 638 792 899 2508
Size: 234 404 154 107 1609
Fragments arranged by size:
1609 404 234 154 107
BssSI C'ACGA_G
Cuts at: 0 1813 2508
Size: 1813 695
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2087 2508
Size: 2087 421
BstEII G'GTnAC_C
Cuts at: 0 783 2508
Size: 783 1725
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 2508
Size: 588 1920
BstYI r'GATC_y
Cuts at: 0 311 2473 2508
Size: 311 2162 35
Fragments arranged by size:
2162 311 35
Bsu36I CC'TnA_GG
Cuts at: 0 1789 2508
Size: 1789 719
BtgI C'CryG_G
Cuts at: 0 867 893 1232 2064 2290 2508
Size: 867 26 339 832 226 218
Fragments arranged by size:
867 832 339 226 218 26
BtsI GCAGTG_nn'
Cuts at: 0 1215 1323 2447 2508
Size: 1215 108 1124 61
Fragments arranged by size:
1215 1124 108 61
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1035 1070 2508
Size: 1035 35 1438
Fragments arranged by size:
1438 1035 35
EaeI y'GGCC_r
Cuts at: 0 807 983 1056 1299 1328 1371 1639 2508
Size: 807 176 73 243 29 43 268 869
Fragments arranged by size:
869 807 268 243 176 73 43 29
EagI C'GGCC_G
Cuts at: 0 807 2508
Size: 807 1701
EarI CTCTTCn'nnn_
Cuts at: 0 29 1228 2042 2318 2508
Size: 29 1199 814 276 190
Fragments arranged by size:
1199 814 276 190 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2087 2508
Size: 2087 421
EcoICRI GAG'CTC
Cuts at: 0 1596 2508
Size: 1596 912
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 445 610 1024 1269 1282 1374 1708
Size: 236 209 165 414 245 13 92 334
Cuts at: 1708 1738 2407 2508
Size: 30 669 101
Fragments arranged by size:
669 414 334 245 236 209 165 101
92 30 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 121 1038 1918 2508
Size: 121 917 880 590
Fragments arranged by size:
917 880 590 121
EcoO109I rG'GnC_Cy
Cuts at: 0 539 540 1125 1179 1555 1645 1729 1796
Size: 539 1 585 54 376 90 84 67
Cuts at: 1796 1906 1907 2162 2169 2243 2304 2354 2355
Size: 110 1 255 7 74 61 50 1
Cuts at: 2355 2508
Size: 153
Fragments arranged by size:
585 539 376 255 153 110 90 84
74 67 61 54 50 7 1 1
1
EcoRI G'AATT_C
Cuts at: 0 444 2508
Size: 444 2064
HaeII r_GCGC'y
Cuts at: 0 522 1874 2508
Size: 522 1352 634
Fragments arranged by size:
1352 634 522
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 296 328 1138 1170 1497 1529 1657 1689
Size: 296 32 810 32 327 32 128 32
Cuts at: 1689 1807 1839 1936 1968 2508
Size: 118 32 97 32 540
Fragments arranged by size:
810 540 327 296 128 118 97 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 285 2508
Size: 285 2223
KasI G'GCGC_C
Cuts at: 0 518 2508
Size: 518 1990
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 2508
Size: 596 1912
MslI CAynn'nnrTG
Cuts at: 0 1234 1613 2508
Size: 1234 379 895
Fragments arranged by size:
1234 895 379
MspA1I CmG'CkG
Cuts at: 0 144 201 995 1289 1882 2066 2084 2508
Size: 144 57 794 294 593 184 18 424
Fragments arranged by size:
794 593 424 294 184 144 57 18
NarI GG'CG_CC
Cuts at: 0 519 2508
Size: 519 1989
NcoI C'CATG_G
Cuts at: 0 893 2508
Size: 893 1615
PasI CC'CwG_GG
Cuts at: 0 1965 2508
Size: 1965 543
PflMI CCAn_nnn'nTGG
Cuts at: 0 981 2508
Size: 981 1527
PfoI T'CCnGG_A
Cuts at: 0 2423 2508
Size: 2423 85
PmlI CAC'GTG
Cuts at: 0 766 1242 2508
Size: 766 476 1266
Fragments arranged by size:
1266 766 476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 2508
Size: 196 32 2280
Fragments arranged by size:
2280 196 32
PpuMI rG'GwC_Cy
Cuts at: 0 1555 1796 2169 2243 2508
Size: 1555 241 373 74 265
Fragments arranged by size:
1555 373 265 241 74
PspOMI G'GGCC_C
Cuts at: 0 539 1179 1644 1729 1906 2162 2354 2508
Size: 539 640 465 85 177 256 192 154
Fragments arranged by size:
640 539 465 256 192 177 154 85
PspXI vC'TCGA_Gb
Cuts at: 0 302 1759 2508
Size: 302 1457 749
Fragments arranged by size:
1457 749 302
PstI C_TGCA'G
Cuts at: 0 159 677 1684 2466 2508
Size: 159 518 1007 782 42
Fragments arranged by size:
1007 782 518 159 42
PvuII CAG'CTG
Cuts at: 0 201 1289 1882 2508
Size: 201 1088 593 626
Fragments arranged by size:
1088 626 593 201
SacI G_AGCT'C
Cuts at: 0 1598 2508
Size: 1598 910
SacII CC_GC'GG
Cuts at: 0 2067 2508
Size: 2067 441
SanDI GG'GwC_CC
Cuts at: 0 2243 2508
Size: 2243 265
SapI GCTCTTCn'nnn_
Cuts at: 0 1228 2508
Size: 1228 1280
SfcI C'TryA_G
Cuts at: 0 155 673 1680 1698 2462 2508
Size: 155 518 1007 18 764 46
Fragments arranged by size:
1007 764 518 155 46 18
SfoI GGC'GCC
Cuts at: 0 520 2508
Size: 520 1988
SmaI CCC'GGG
Cuts at: 0 1177 2160 2167 2320 2501 2508
Size: 1177 983 7 153 181 7
Fragments arranged by size:
1177 983 181 153 7 7
SmlI C'TyrA_G
Cuts at: 0 226 302 1759 2508
Size: 226 76 1457 749
Fragments arranged by size:
1457 749 226 76
StyI C'CwwG_G
Cuts at: 0 117 389 893 1070 1636 2238 2508
Size: 117 272 504 177 566 602 270
Fragments arranged by size:
602 566 504 272 270 177 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 20 2484 2508
Size: 20 2464 24
Fragments arranged by size:
2464 24 20
TatI w'GTAC_w
Cuts at: 0 138 596 631 2120 2508
Size: 138 458 35 1489 388
Fragments arranged by size:
1489 458 388 138 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1372 1404 2508
Size: 1372 32 1104
Fragments arranged by size:
1372 1104 32
Tth111I GACn'n_nGTC
Cuts at: 0 437 2508
Size: 437 2071
XbaI T'CTAG_A
Cuts at: 0 474 2508
Size: 474 2034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 1398 1683 2508
Size: 456 942 285 825
Fragments arranged by size:
942 825 456 285
XhoI C'TCGA_G
Cuts at: 0 302 1759 2508
Size: 302 1457 749
Fragments arranged by size:
1457 749 302
XmaI C'CCGG_G
Cuts at: 0 1175 2158 2165 2318 2499 2508
Size: 1175 983 7 153 181 9
Fragments arranged by size:
1175 983 181 153 9 7
Enzymes that do cut:
AarI AccI AcuI AgeI AleI AlwNI ApaI ApaLI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII KasI MmeI MslI MspA1I NarI NcoI PasI
PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuII SacI SacII SanDI SapI SfcI SfoI SmaI
SmlI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AlfI
AloI AscI AseI AsiSI BaeI BcgI BfrBI BlpI
BmtI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII
BstBI BstZ17I BtgZI ClaI DraI DraIII DrdI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MscI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PshAI PsiI PsrI
PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
XmnI ZraI