(Circular) (Six-base) MAPSORT of: pFN21ASDA1089.seq Check: 6012 from: 1 to: 7777 pFN21ASDA1089 7777 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4895 4895 Size: 7777 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5587 278 Size: 53 83 186 936 4051 2468 Fragments arranged by size: 4051 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2280 3858 5120 1937 Size: 343 1578 1262 4594 Fragments arranged by size: 4594 1578 1262 343 Acc65I G'GTAC_C Cuts at: 4743 5098 4743 Size: 355 7422 AclI AA'CG_TT Cuts at: 1563 5904 6277 1563 Size: 4341 373 3063 Fragments arranged by size: 4341 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2840 3852 3944 4505 4727 4847 5840 7224 Size: 1208 1012 92 561 222 120 993 1384 Cuts at: 7224 7492 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1208 1012 993 561 268 222 120 92 AfeI AGC'GCT Cuts at: 3758 3758 Size: 7777 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7580 AflIII A'CryG_T Cuts at: 1203 6676 7653 1203 Size: 5473 977 1327 Fragments arranged by size: 5473 1327 977 AgeI A'CCGG_T Cuts at: 6608 6608 Size: 7777 AhdI GACnn_n'nnGTC Cuts at: 6506 6506 Size: 7777 AleI CACnn'nnGTG Cuts at: 1166 5016 1166 Size: 3850 3927 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2385 3171 4388 5022 1184 Size: 1201 786 1217 634 3939 Fragments arranged by size: 3939 1217 1201 786 634 AlwNI CAG_nnn'CTG Cuts at: 2495 3326 3881 3967 5520 7092 2495 Size: 831 555 86 1553 1572 3180 Fragments arranged by size: 3180 1572 1553 831 555 86 ApaI G_GGCC'C Cuts at: 3695 4729 3695 Size: 1034 6743 ApaLI G'TGCA_C Cuts at: 3119 5834 6990 3119 Size: 2715 1156 3906 Fragments arranged by size: 3906 2715 1156 ApoI r'AATT_y Cuts at: 1493 5086 5312 5464 7573 1493 Size: 3593 226 152 2109 1697 Fragments arranged by size: 3593 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6331 160 Size: 872 5299 1606 Fragments arranged by size: 5299 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7777 AvaI C'yCGr_G Cuts at: 1870 1943 3483 3648 4728 5102 1870 Size: 73 1540 165 1080 374 4545 Fragments arranged by size: 4545 1540 1080 374 165 73 BamHI G'GATC_C Cuts at: 3140 4915 5107 5474 3140 Size: 1775 192 367 5443 Fragments arranged by size: 5443 1775 367 192 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2678 4743 5098 6553 618 Size: 325 217 627 891 2065 355 1455 1842 Fragments arranged by size: 2065 1842 1455 891 627 355 325 217 BanII G_rGCy'C Cuts at: 1391 2061 2208 3500 3645 3695 3893 4058 4729 Size: 670 147 1292 145 50 198 165 671 Cuts at: 4729 5096 1391 Size: 367 4072 Fragments arranged by size: 4072 1292 671 670 367 198 165 147 145 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3994 928 Size: 971 2095 4711 Fragments arranged by size: 4711 2095 971 BbvCI CC'TCA_GC Cuts at: 2256 3020 2256 Size: 764 7013 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5968 6002 1903 Size: 34 4031 34 3678 Fragments arranged by size: 4031 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5669 6879 5669 Size: 1210 6567 BclI T'GATC_A Cuts at: 1548 1548 Size: 7777 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3690 4362 4901 Size: 107 122 71 631 605 2018 672 539 Cuts at: 4901 5000 6388 136 Size: 99 1388 1525 Fragments arranged by size: 2018 1525 1388 672 631 605 539 122 107 99 71 BglII A'GATC_T Cuts at: 4156 7772 4156 Size: 3616 4161 BlpI GC'TnA_GC Cuts at: 3225 4289 4509 5194 3225 Size: 1064 220 685 5808 Fragments arranged by size: 5808 1064 685 220 Bme1580I G_kGCm'C Cuts at: 1790 2524 2681 3123 3695 4729 5838 6994 1790 Size: 734 157 442 572 1034 1109 1156 2573 Fragments arranged by size: 2573 1156 1109 1034 734 572 442 157 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7777 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2228 3690 3698 Size: 416 53 83 399 24 811 1462 8 Cuts at: 3698 4408 4564 5508 6466 442 Size: 710 156 944 958 1753 Fragments arranged by size: 1753 1462 958 944 811 710 416 399 156 83 53 24 8 BmtI G_CTAG'C Cuts at: 1056 2612 3708 1056 Size: 1556 1096 5125 Fragments arranged by size: 5125 1556 1096 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2968 3895 3907 2968 Size: 927 12 6838 Fragments arranged by size: 6838 927 12 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2993 3659 3782 4103 4562 4769 6437 1239 Size: 1754 666 123 321 459 207 1668 2579 Fragments arranged by size: 2579 1754 1668 666 459 321 207 123 Bpu10I CC'TnA_GC Cuts at: 2256 3020 3327 2256 Size: 764 307 6706 Fragments arranged by size: 6706 764 307 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2762 3451 5902 6767 7065 2762 Size: 689 2451 865 298 3474 Fragments arranged by size: 3474 2451 865 689 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6440 882 Size: 617 4941 2219 Fragments arranged by size: 4941 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5074 5568 493 Size: 711 3870 494 2702 Fragments arranged by size: 3870 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7771 7771 Size: 7777 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5584 5966 6601 275 Size: 53 83 186 936 4051 382 635 1451 Fragments arranged by size: 4051 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6210 6608 6882 7029 7440 6210 Size: 398 274 147 411 6547 Fragments arranged by size: 6547 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2252 2282 2990 3020 4742 Size: 30 165 30 849 30 708 30 1722 Cuts at: 4742 4772 1178 Size: 30 4183 Fragments arranged by size: 4183 1722 849 708 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2273 2637 2998 3056 3059 3324 3326 3329 Size: 1087 364 361 58 3 265 2 3 Cuts at: 3329 3629 3785 3899 4316 4925 1186 Size: 300 156 114 417 609 4038 Fragments arranged by size: 4038 1087 609 417 364 361 300 265 156 114 58 3 3 2 BseYI C'CCAG_C Cuts at: 2674 4964 6980 7476 2674 Size: 2290 2016 496 2975 Fragments arranged by size: 2975 2290 2016 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2213 3177 1163 Size: 573 477 964 5763 Fragments arranged by size: 5763 964 573 477 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5227 5988 7016 1540 Size: 275 186 3226 761 1028 2301 Fragments arranged by size: 3226 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 2339 3123 4058 5838 5923 6994 2208 Size: 131 784 935 1780 85 1071 2991 Fragments arranged by size: 2991 1780 1071 935 784 131 85 BsmI GAATG_Cn' Cuts at: 2664 3257 5288 5381 2664 Size: 593 2031 93 5060 Fragments arranged by size: 5060 2031 593 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2208 2339 2524 2681 3123 3500 Size: 399 271 147 131 185 157 442 377 Cuts at: 3500 3645 3695 3893 4058 4729 5096 5838 5923 Size: 145 50 198 165 671 367 742 85 Cuts at: 5923 6994 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 671 442 399 377 367 271 198 185 165 157 147 145 131 85 50 BspEI T'CCGG_A Cuts at: 7440 7440 Size: 7777 BspHI T'CATG_A Cuts at: 4494 5666 7396 4494 Size: 1172 1730 4875 Fragments arranged by size: 4875 1730 1172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2736 3009 3798 4239 4895 5132 844 Size: 1892 273 789 441 656 237 3489 Fragments arranged by size: 3489 1892 789 656 441 273 237 BsrBI CCG'CTC Cuts at: 1394 1823 4240 4816 5664 1394 Size: 429 2417 576 848 3507 Fragments arranged by size: 3507 2417 848 576 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3162 3708 4407 6272 6446 65 Size: 1183 1914 546 699 1865 174 1396 Fragments arranged by size: 1914 1865 1396 1183 699 546 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6421 6608 1700 Size: 110 4611 187 2869 Fragments arranged by size: 4611 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7777 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7777 BssSI C'ACGA_G Cuts at: 5837 6849 5837 Size: 1012 6765 BstAPI GCAn_nnn'nTGC Cuts at: 794 3164 4323 794 Size: 2370 1159 4248 Fragments arranged by size: 4248 2370 1159 BstBI TT'CG_AA Cuts at: 2007 6581 2007 Size: 4574 3203 BstEII G'GTnAC_C Cuts at: 1184 3627 4930 1184 Size: 2443 1303 4031 Fragments arranged by size: 4031 2443 1303 BstXI CCAn_nnnn'nTGG Cuts at: 3269 4127 3269 Size: 858 6919 BstYI r'GATC_y Cuts at: 1972 3140 3322 4156 4863 4915 5107 5474 5859 Size: 1168 182 834 707 52 192 367 385 Cuts at: 5859 5876 7328 7414 7426 7772 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1168 834 707 385 367 346 192 182 86 52 17 12 BstZ17I GTA'TAC Cuts at: 2281 2281 Size: 7777 Bsu36I CC'TnA_GG Cuts at: 2186 2500 4146 2186 Size: 314 1646 5817 Fragments arranged by size: 5817 1646 314 BtgI C'CryG_G Cuts at: 513 1065 2934 513 Size: 552 1869 5356 Fragments arranged by size: 5356 1869 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2875 4333 487 Size: 683 56 1649 1458 3931 Fragments arranged by size: 3931 1649 1458 683 56 BtsI GCAGTG_nn' Cuts at: 2441 5060 5293 6087 6107 2441 Size: 2619 233 794 20 4111 Fragments arranged by size: 4111 2619 794 233 20 ClaI AT'CG_AT Cuts at: 1554 4689 1554 Size: 3135 4642 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3821 3856 4049 4084 529 Size: 35 3257 35 193 35 4222 Fragments arranged by size: 4222 3257 193 35 35 35 DraI TTT'AAA Cuts at: 4762 5081 4762 Size: 319 7458 DraIII CAC_nnn'GTG Cuts at: 2236 2236 Size: 7777 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3927 6784 809 Size: 507 2611 2857 1802 Fragments arranged by size: 2857 2611 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3027 3825 3972 Size: 54 1425 50 275 18 1197 798 147 Cuts at: 3972 4644 4654 5224 6113 7742 8 Size: 672 10 570 889 1629 43 Fragments arranged by size: 1629 1425 1197 889 798 672 570 275 147 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 5224 1537 Size: 275 3412 4090 Fragments arranged by size: 4090 3412 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2999 3228 3606 4227 4823 5707 1353 Size: 1646 229 378 621 596 884 3423 Fragments arranged by size: 3423 1646 884 621 596 378 229 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3389 4625 6364 6738 6884 387 Size: 1064 138 1800 1236 1739 374 146 1280 Fragments arranged by size: 1800 1739 1280 1236 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2206 4056 2206 Size: 1850 5927 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2840 2993 3659 3782 3852 3944 4103 Size: 393 1208 153 666 123 70 92 159 Cuts at: 4103 4505 4562 4727 4769 4847 5840 6437 7224 Size: 402 57 165 42 78 993 597 787 Cuts at: 7224 7492 1239 Size: 268 1524 Fragments arranged by size: 1524 1208 993 787 666 597 402 393 268 165 159 153 123 92 78 70 57 42 EcoO109I rG'GnC_Cy Cuts at: 2033 3692 3964 4609 4725 4979 2033 Size: 1659 272 645 116 254 4831 Fragments arranged by size: 4831 1659 645 272 254 116 EcoRI G'AATT_C Cuts at: 5086 5464 5086 Size: 378 7399 EcoRV GAT'ATC Cuts at: 2573 2573 Size: 7777 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3778 4001 4178 3778 Size: 223 177 7377 Fragments arranged by size: 7377 223 177 FspI TGC'GCA Cuts at: 4050 4316 6283 4050 Size: 266 1967 5544 Fragments arranged by size: 5544 1967 266 FspAI rTGC'GCAy Cuts at: 4316 4316 Size: 7777 HaeII r_GCGC'y Cuts at: 1678 3760 6924 1678 Size: 2082 3164 2531 Fragments arranged by size: 3164 2531 2082 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2112 2144 2518 2550 3494 Size: 32 135 32 540 32 374 32 944 Cuts at: 3494 3526 3572 3604 4175 4207 4509 4541 6418 Size: 32 46 32 571 32 302 32 1877 Cuts at: 6418 6450 6492 6524 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1877 944 571 540 374 302 135 46 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3208 5121 5367 669 Size: 988 281 1270 1913 246 3079 Fragments arranged by size: 3079 1913 1270 988 281 246 HindIII A'AGCT_T Cuts at: 748 2216 3956 748 Size: 1468 1740 4569 Fragments arranged by size: 4569 1740 1468 HpaI GTT'AAC Cuts at: 5367 5367 Size: 7777 KpnI G_GTAC'C Cuts at: 4747 5102 4747 Size: 355 7422 MfeI C'AATT_G Cuts at: 5376 5376 Size: 7777 MluI A'CGCG_T Cuts at: 7653 7653 Size: 7777 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3790 3879 3911 4463 6891 7075 1305 Size: 2485 89 32 552 2428 184 2007 Fragments arranged by size: 2485 2428 2007 552 184 89 32 MscI TGG'CCA Cuts at: 10 64 1489 1832 3029 4646 10 Size: 54 1425 343 1197 1617 3141 Fragments arranged by size: 3141 1617 1425 1197 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3740 4040 5016 5735 6094 6253 518 Size: 648 2574 300 976 719 359 159 2042 Fragments arranged by size: 2574 2042 976 719 648 359 300 159 MspA1I CmG'CkG Cuts at: 1606 2425 2674 4540 4875 4885 5006 5193 5870 Size: 819 249 1866 335 10 121 187 677 Cuts at: 5870 7018 7263 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1866 1148 819 677 335 249 245 187 121 10 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7225 NdeI CA'TA_TG Cuts at: 387 387 Size: 7777 NheI G'CTAG_C Cuts at: 1052 2608 3704 1052 Size: 1556 1096 5125 Fragments arranged by size: 5125 1556 1096 NotI GC'GGCC_GC Cuts at: 5224 5224 Size: 7777 NspI r_CATG'y Cuts at: 1132 1229 2535 5135 6680 1132 Size: 97 1306 2600 1545 2229 Fragments arranged by size: 2600 2229 1545 1306 97 PasI CC'CwG_GG Cuts at: 1348 4721 4897 1348 Size: 3373 176 4228 Fragments arranged by size: 4228 3373 176 PciI A'CATG_T Cuts at: 6676 6676 Size: 7777 PflMI CCAn_nnn'nTGG Cuts at: 1108 4391 1108 Size: 3283 4494 PfoI T'CCnGG_A Cuts at: 2970 3072 3948 4005 4119 2970 Size: 102 876 57 114 6628 Fragments arranged by size: 6628 876 114 102 57 PmeI GTTT'AAAC Cuts at: 5081 5081 Size: 7777 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5846 5878 7359 7391 5846 Size: 32 1481 32 6232 Fragments arranged by size: 6232 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3964 3964 Size: 7777 PshAI GACnn'nnGTC Cuts at: 1613 2456 3519 1613 Size: 843 1063 5871 Fragments arranged by size: 5871 1063 843 PsiI TTA'TAA Cuts at: 5347 5347 Size: 7777 PspOMI G'GGCC_C Cuts at: 3691 4725 3691 Size: 1034 6743 PstI C_TGCA'G Cuts at: 830 2436 3222 4377 4440 4557 5001 5129 830 Size: 1606 786 1155 63 117 444 128 3478 Fragments arranged by size: 3478 1606 1155 786 444 128 117 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7777 PvuII CAG'CTG Cuts at: 2425 2674 4540 4875 4885 2425 Size: 249 1866 335 10 5317 Fragments arranged by size: 5317 1866 335 249 10 SacI G_AGCT'C Cuts at: 2208 4058 2208 Size: 1850 5927 SalI G'TCGA_C Cuts at: 1936 5119 1936 Size: 3183 4594 SapI GCTCTTCn'nnn_ Cuts at: 4227 4227 Size: 7777 SbfI CC_TGCA'GG Cuts at: 5001 5129 5001 Size: 128 7649 ScaI AGT'ACT Cuts at: 2699 6025 7737 2699 Size: 3326 1712 2739 Fragments arranged by size: 3326 2739 1712 SexAI A'CCwGG_T Cuts at: 2364 2364 Size: 7777 SfcI C'TryA_G Cuts at: 826 1045 2353 2432 2915 3218 4373 4436 4553 Size: 219 1308 79 483 303 1155 63 117 Cuts at: 4553 4997 5125 6260 6941 7132 826 Size: 444 128 1135 681 191 1471 Fragments arranged by size: 1471 1308 1155 1135 681 483 444 303 219 191 128 117 79 63 SfiI GGCCn_nnn'nGGCC Cuts at: 3690 3690 Size: 7777 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7777 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7777 SmaI CCC'GGG Cuts at: 1872 3485 4730 5104 1872 Size: 1613 1245 374 4545 Fragments arranged by size: 4545 1613 1245 374 SmlI C'TyrA_G Cuts at: 820 1017 1943 2777 3466 5881 6782 7044 820 Size: 197 926 834 689 2415 901 262 1553 Fragments arranged by size: 2415 1553 926 901 834 689 262 197 SnaBI TAC'GTA Cuts at: 493 5074 5568 493 Size: 4581 494 2702 Fragments arranged by size: 4581 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7777 SphI G_CATG'C Cuts at: 1132 5135 1132 Size: 4003 3774 SrfI GCCC'GGGC Cuts at: 3485 3485 Size: 7777 SspI AAT'ATT Cuts at: 5 52 5701 5 Size: 47 5649 2081 Fragments arranged by size: 5649 2081 47 StuI AGG'CCT Cuts at: 1517 3838 4947 1517 Size: 2321 1109 4347 Fragments arranged by size: 4347 2321 1109 StyI C'CwwG_G Cuts at: 513 1065 2029 3291 4563 4676 5216 513 Size: 552 964 1262 1272 113 540 3074 Fragments arranged by size: 3074 1272 1262 964 552 540 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2247 3028 3344 3429 3433 3752 4619 Size: 1255 667 781 316 85 4 319 867 Cuts at: 4619 4734 5804 5821 5974 6159 6604 325 Size: 115 1070 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1070 867 781 667 445 319 316 185 153 115 85 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2697 3150 6023 Size: 274 80 33 51 1443 720 453 2873 Cuts at: 6023 7735 96 Size: 1712 138 Fragments arranged by size: 2873 1712 1443 720 453 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2252 2284 2420 2452 4933 4965 7737 7769 2252 Size: 32 136 32 2481 32 2772 32 2260 Fragments arranged by size: 2772 2481 2260 136 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3625 1316 Size: 2309 5468 XbaI T'CTAG_A Cuts at: 5113 5113 Size: 7777 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3022 3298 3022 Size: 276 7501 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7777 XmaI C'CCGG_G Cuts at: 1870 3483 4728 5102 1870 Size: 1613 1245 374 4545 Fragments arranged by size: 4545 1613 1245 374 XmnI GAAnn'nnTTC Cuts at: 4001 5906 4001 Size: 1905 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5585 276 Size: 53 83 186 936 4051 2468 Fragments arranged by size: 4051 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI EcoNI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfoI SwaI