Restriction map for pFN21ASDA1089
 (Linear) (Six-base) MAPSORT of: KIAA1089.seq  Check: 5672  from: 1  to: 3048



KIAA1089.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2872    3048 
   Size:       2872     176
 
AccI GT'mk_AC

Cuts at:      0     257    1835    3048 
   Size:        257    1578    1213

  Fragments arranged by size: 

                1578    1213     257
 
Acc65I G'GTAC_C

Cuts at:      0    2720    3048 
   Size:       2720     328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     817    1829    1921    2482    2704    2824    3048 
   Size:        817    1012      92     561     222     120     224

  Fragments arranged by size: 

                1012     817     561     224     222     120      92
 
AfeI AGC'GCT

Cuts at:      0    1735    3048 
   Size:       1735    1313
 
AleI CACnn'nnGTG

Cuts at:      0    2993    3048 
   Size:       2993      55
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     362    1148    2365    2999    3048 
   Size:        362     786    1217     634      49

  Fragments arranged by size: 

                1217     786     634     362      49
 
AlwNI CAG_nnn'CTG

Cuts at:      0     472    1303    1858    1944    3048 
   Size:        472     831     555      86    1104

  Fragments arranged by size: 

                1104     831     555     472      86
 
ApaI G_GGCC'C

Cuts at:      0    1672    2706    3048 
   Size:       1672    1034     342

  Fragments arranged by size: 

                1672    1034     342
 
ApaLI G'TGCA_C

Cuts at:      0    1096    3048 
   Size:       1096    1952
 
AvaI C'yCGr_G

Cuts at:      0    1460    1625    2705    3048 
   Size:       1460     165    1080     343

  Fragments arranged by size: 

                1460    1080     343     165
 
BamHI G'GATC_C

Cuts at:      0    1117    2892    3048 
   Size:       1117    1775     156

  Fragments arranged by size: 

                1775    1117     156
 
BanI G'GyrC_C

Cuts at:      0     655    2720    3048 
   Size:        655    2065     328

  Fragments arranged by size: 

                2065     655     328
 
BanII G_rGCy'C

Cuts at:      0      38     185    1477    1622    1672    1870    2035    2706 
   Size:         38     147    1292     145      50     198     165     671

Cuts at:   2706    3048 
   Size:        342

  Fragments arranged by size: 

                1292     671     342     198     165     147     145      50
                  38
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1971    3048 
   Size:       1971    1077
 
BbvCI CC'TCA_GC

Cuts at:      0     233     997    3048 
   Size:        233     764    2051

  Fragments arranged by size: 

                2051     764     233
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1667    2339    2878    2977    3048 
   Size:       1667     672     539      99      71

  Fragments arranged by size: 

                1667     672     539      99      71
 
BglII A'GATC_T

Cuts at:      0    2133    3048 
   Size:       2133     915
 
BlpI GC'TnA_GC

Cuts at:      0    1202    2266    2486    3048 
   Size:       1202    1064     220     562

  Fragments arranged by size: 

                1202    1064     562     220
 
Bme1580I G_kGCm'C

Cuts at:      0     501     658    1100    1672    2706    3048 
   Size:        501     157     442     572    1034     342

  Fragments arranged by size: 

                1034     572     501     442     342     157
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     205    1667    1675    2385    2541    3048 
   Size:        205    1462       8     710     156     507

  Fragments arranged by size: 

                1462     710     507     205     156       8
 
BmtI G_CTAG'C

Cuts at:      0     589    1685    3048 
   Size:        589    1096    1363

  Fragments arranged by size: 

                1363    1096     589
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     945    1872    1884    3048 
   Size:        945     927      12    1164

  Fragments arranged by size: 

                1164     945     927      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     970    1636    1759    2080    2539    2746    3048 
   Size:        970     666     123     321     459     207     302

  Fragments arranged by size: 

                 970     666     459     321     302     207     123
 
Bpu10I CC'TnA_GC

Cuts at:      0     233     997    1304    3048 
   Size:        233     764     307    1744

  Fragments arranged by size: 

                1744     764     307     233
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739    1428    3048 
   Size:        739     689    1620

  Fragments arranged by size: 

                1620     739     689
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     229     259     967     997    2719    2749    3048 
   Size:        229      30     708      30    1722      30     299

  Fragments arranged by size: 

                1722     708     299     229      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     614     975    1033    1036    1301    1303    1306 
   Size:        250     364     361      58       3     265       2       3

Cuts at:   1306    1606    1762    1876    2293    2902    3048 
   Size:        300     156     114     417     609     146

  Fragments arranged by size: 

                 609     417     364     361     300     265     250     156
                 146     114      58       3       3       2
 
BseYI C'CCAG_C

Cuts at:      0     651    2941    3048 
   Size:        651    2290     107

  Fragments arranged by size: 

                2290     651     107
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190    1154    3048 
   Size:        190     964    1894

  Fragments arranged by size: 

                1894     964     190
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     316    1100    2035    3048 
   Size:        185     131     784     935    1013

  Fragments arranged by size: 

                1013     935     784     185     131
 
BsmI GAATG_Cn'

Cuts at:      0     641    1234    3048 
   Size:        641     593    1814

  Fragments arranged by size: 

                1814     641     593
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     185     316     501     658    1100    1477    1622 
   Size:         38     147     131     185     157     442     377     145

Cuts at:   1622    1672    1870    2035    2706    3048 
   Size:         50     198     165     671     342

  Fragments arranged by size: 

                 671     442     377     342     198     185     165     157
                 147     145     131      50      38
 
BspHI T'CATG_A

Cuts at:      0    2471    3048 
   Size:       2471     577
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     713     986    1775    2216    2872    3048 
   Size:        713     273     789     441     656     176

  Fragments arranged by size: 

                 789     713     656     441     273     176
 
BsrBI CCG'CTC

Cuts at:      0    2217    2793    3048 
   Size:       2217     576     255

  Fragments arranged by size: 

                2217     576     255
 
BsrDI GCAATG_nn'

Cuts at:      0    1139    1685    2384    3048 
   Size:       1139     546     699     664

  Fragments arranged by size: 

                1139     699     664     546
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1141    2300    3048 
   Size:       1141    1159     748

  Fragments arranged by size: 

                1159    1141     748
 
BstEII G'GTnAC_C

Cuts at:      0    1604    2907    3048 
   Size:       1604    1303     141

  Fragments arranged by size: 

                1604    1303     141
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1246    2104    3048 
   Size:       1246     858     944

  Fragments arranged by size: 

                1246     944     858
 
BstYI r'GATC_y

Cuts at:      0    1117    1299    2133    2840    2892    3048 
   Size:       1117     182     834     707      52     156

  Fragments arranged by size: 

                1117     834     707     182     156      52
 
BstZ17I GTA'TAC

Cuts at:      0     258    3048 
   Size:        258    2790
 
Bsu36I CC'TnA_GG

Cuts at:      0     163     477    2123    3048 
   Size:        163     314    1646     925

  Fragments arranged by size: 

                1646     925     314     163
 
BtgI C'CryG_G

Cuts at:      0     911    3048 
   Size:        911    2137
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     852    2310    3048 
   Size:        852    1458     738

  Fragments arranged by size: 

                1458     852     738
 
BtsI GCAGTG_nn'

Cuts at:      0     418    3037    3048 
   Size:        418    2619      11

  Fragments arranged by size: 

                2619     418      11
 
ClaI AT'CG_AT

Cuts at:      0    2666    3048 
   Size:       2666     382
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1798    1833    2026    2061    3048 
   Size:       1798      35     193      35     987

  Fragments arranged by size: 

                1798     987     193      35      35
 
DraI TTT'AAA

Cuts at:      0    2739    3048 
   Size:       2739     309
 
DraIII CAC_nnn'GTG

Cuts at:      0     213    3048 
   Size:        213    2835
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1904    3048 
   Size:       1904    1144
 
EaeI y'GGCC_r

Cuts at:      0    1004    1802    1949    2621    2631    3048 
   Size:       1004     798     147     672      10     417

  Fragments arranged by size: 

                1004     798     672     417     147      10
 
EarI CTCTTCn'nnn_

Cuts at:      0     976    1205    1583    2204    2800    3048 
   Size:        976     229     378     621     596     248

  Fragments arranged by size: 

                 976     621     596     378     248     229
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1366    2602    3048 
   Size:       1366    1236     446

  Fragments arranged by size: 

                1366    1236     446
 
EcoICRI GAG'CTC

Cuts at:      0     183    2033    3048 
   Size:        183    1850    1015

  Fragments arranged by size: 

                1850    1015     183
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     817     970    1636    1759    1829    1921    2080    2482 
   Size:        817     153     666     123      70      92     159     402

Cuts at:   2482    2539    2704    2746    2824    3048 
   Size:         57     165      42      78     224

  Fragments arranged by size: 

                 817     666     402     224     165     159     153     123
                  92      78      70      57      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0      10    1669    1941    2586    2702    2956    3048 
   Size:         10    1659     272     645     116     254      92

  Fragments arranged by size: 

                1659     645     272     254     116      92      10
 
EcoRV GAT'ATC

Cuts at:      0     550    3048 
   Size:        550    2498
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1755    1978    2155    3048 
   Size:       1755     223     177     893

  Fragments arranged by size: 

                1755     893     223     177
 
FspI TGC'GCA

Cuts at:      0    2027    2293    3048 
   Size:       2027     266     755

  Fragments arranged by size: 

                2027     755     266
 
FspAI rTGC'GCAy

Cuts at:      0    2293    3048 
   Size:       2293     755
 
HaeII r_GCGC'y

Cuts at:      0    1737    3048 
   Size:       1737    1311
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      89     121     495     527    1471    1503    1549    1581 
   Size:         89      32     374      32     944      32      46      32

Cuts at:   1581    2152    2184    2486    2518    3048 
   Size:        571      32     302      32     530

  Fragments arranged by size: 

                 944     571     530     374     302      89      46      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1185    3048 
   Size:       1185    1863
 
HindIII A'AGCT_T

Cuts at:      0     193    1933    3048 
   Size:        193    1740    1115

  Fragments arranged by size: 

                1740    1115     193
 
KpnI G_GTAC'C

Cuts at:      0    2724    3048 
   Size:       2724     324
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1767    1856    1888    2440    3048 
   Size:       1767      89      32     552     608

  Fragments arranged by size: 

                1767     608     552      89      32
 
MscI TGG'CCA

Cuts at:      0    1006    2623    3048 
   Size:       1006    1617     425

  Fragments arranged by size: 

                1617    1006     425
 
MslI CAynn'nnrTG

Cuts at:      0    1717    2017    2993    3048 
   Size:       1717     300     976      55

  Fragments arranged by size: 

                1717     976     300      55
 
MspA1I CmG'CkG

Cuts at:      0     402     651    2517    2852    2862    2983    3048 
   Size:        402     249    1866     335      10     121      65

  Fragments arranged by size: 

                1866     402     335     249     121      65      10
 
NheI G'CTAG_C

Cuts at:      0     585    1681    3048 
   Size:        585    1096    1367

  Fragments arranged by size: 

                1367    1096     585
 
NspI r_CATG'y

Cuts at:      0     512    3048 
   Size:        512    2536
 
PasI CC'CwG_GG

Cuts at:      0    2698    2874    3048 
   Size:       2698     176     174

  Fragments arranged by size: 

                2698     176     174
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2368    3048 
   Size:       2368     680
 
PfoI T'CCnGG_A

Cuts at:      0     947    1049    1925    1982    2096    3048 
   Size:        947     102     876      57     114     952

  Fragments arranged by size: 

                 952     947     876     114     102      57
 
PpuMI rG'GwC_Cy

Cuts at:      0    1941    3048 
   Size:       1941    1107
 
PshAI GACnn'nnGTC

Cuts at:      0     433    1496    3048 
   Size:        433    1063    1552

  Fragments arranged by size: 

                1552    1063     433
 
PspOMI G'GGCC_C

Cuts at:      0    1668    2702    3048 
   Size:       1668    1034     346

  Fragments arranged by size: 

                1668    1034     346
 
PstI C_TGCA'G

Cuts at:      0     413    1199    2354    2417    2534    2978    3048 
   Size:        413     786    1155      63     117     444      70

  Fragments arranged by size: 

                1155     786     444     413     117      70      63
 
PvuII CAG'CTG

Cuts at:      0     402     651    2517    2852    2862    3048 
   Size:        402     249    1866     335      10     186

  Fragments arranged by size: 

                1866     402     335     249     186      10
 
SacI G_AGCT'C

Cuts at:      0     185    2035    3048 
   Size:        185    1850    1013

  Fragments arranged by size: 

                1850    1013     185
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2204    3048 
   Size:       2204     844
 
SbfI CC_TGCA'GG

Cuts at:      0    2978    3048 
   Size:       2978      70
 
ScaI AGT'ACT

Cuts at:      0     676    3048 
   Size:        676    2372
 
SexAI A'CCwGG_T

Cuts at:      0     341    3048 
   Size:        341    2707
 
SfcI C'TryA_G

Cuts at:      0     330     409     892    1195    2350    2413    2530    2974 
   Size:        330      79     483     303    1155      63     117     444

Cuts at:   2974    3048 
   Size:         74

  Fragments arranged by size: 

                1155     483     444     330     303     117      79      74
                  63
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1667    3048 
   Size:       1667    1381
 
SmaI CCC'GGG

Cuts at:      0    1462    2707    3048 
   Size:       1462    1245     341

  Fragments arranged by size: 

                1462    1245     341
 
SmlI C'TyrA_G

Cuts at:      0     754    1443    3048 
   Size:        754     689    1605

  Fragments arranged by size: 

                1605     754     689
 
SrfI GCCC'GGGC

Cuts at:      0    1462    3048 
   Size:       1462    1586
 
StuI AGG'CCT

Cuts at:      0    1815    2924    3048 
   Size:       1815    1109     124

  Fragments arranged by size: 

                1815    1109     124
 
StyI C'CwwG_G

Cuts at:      0       6    1268    2540    2653    3048 
   Size:          6    1262    1272     113     395

  Fragments arranged by size: 

                1272    1262     395     113       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     224    1005    1321    1406    1410    1729    2596    2711 
   Size:        224     781     316      85       4     319     867     115

Cuts at:   2711    3048 
   Size:        337

  Fragments arranged by size: 

                 867     781     337     319     316     224     115      85
                   4
 
TatI w'GTAC_w

Cuts at:      0     674    1127    3048 
   Size:        674     453    1921

  Fragments arranged by size: 

                1921     674     453
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     229     261     397     429    2910    2942    3048 
   Size:        229      32     136      32    2481      32     106

  Fragments arranged by size: 

                2481     229     136     106      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1602    3048 
   Size:       1602    1446
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     999    1275    3048 
   Size:        999     276    1773

  Fragments arranged by size: 

                1773     999     276
 
XmaI C'CCGG_G

Cuts at:      0    1460    2705    3048 
   Size:       1460    1245     343

  Fragments arranged by size: 

                1460    1245     343
 
XmnI GAAnn'nnTTC

Cuts at:      0    1978    3048 
   Size:       1978    1070
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI     AleI     AlfI    AlwNI
     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI   BstAPI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRV     FalI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NheI     NspI
     PasI    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SacI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SmaI
     SmlI     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII   AflIII     AgeI     AhdI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI
     BclI    BfrBI    BmgBI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsiEI    BsiWI    BsmBI    BspEI    BsrFI    BsrGI   BssHII    BssSI
    BstBI     EagI    EcoNI    EcoRI     FseI     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SspI     SwaI     XbaI     XhoI
     ZraI
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