Restriction map for pFN21ASDA0921
 (Circular) (Six-base) MAPSORT of: pFN21ASDA0921.seq  Check: 5602  from: 1  to: 9655



pFN21ASDA0921  9655 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2446    5155    5207    5267    5611    5829    2446 
   Size:       2709      52      60     344     218    6272

  Fragments arranged by size: 

                6272    2709     344     218      60      52
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3078    5125    7465     278 
   Size:         53      83     186     936    1542    2047    2340    2468

  Fragments arranged by size: 

                2468    2340    2047    1542     936     186      83      53
            
 
AccI GT'mk_AC

Cuts at:   1937    6948    6998    1937 
   Size:       5011      50    4594

  Fragments arranged by size: 

                5011    4594      50
 
Acc65I G'GTAC_C

Cuts at:   5649    6976    5649 
   Size:       1327    8328
 
AclI AA'CG_TT

Cuts at:   1563    7782    8155    1563 
   Size:       6219     373    3063

  Fragments arranged by size: 

                6219    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2170    2588    3508    4022    5226    5392    6509    7718 
   Size:        538     418     920     514    1204     166    1117    1209

Cuts at:   7718    9102    9370    1632 
   Size:       1384     268    1917

  Fragments arranged by size: 

                1917    1384    1209    1204    1117     920     538     514
                 418     268     166
 
AfeI AGC'GCT

Cuts at:   3460    5340    5428    3460 
   Size:       1880      88    7687

  Fragments arranged by size: 

                7687    1880      88
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    9458
 
AflIII A'CryG_T

Cuts at:   1203    4833    5352    8554    9531    1203 
   Size:       3630     519    3202     977    1327

  Fragments arranged by size: 

                3630    3202    1327     977     519
 
AgeI A'CCGG_T

Cuts at:   8486    8486 
   Size:       9655
 
AhdI GACnn_n'nnGTC

Cuts at:   5814    8384    5814 
   Size:       2570    7085
 
AleI CACnn'nnGTG

Cuts at:   1166    2339    6176    1166 
   Size:       1173    3837    4645

  Fragments arranged by size: 

                4645    3837    1173
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3671    4421    1184 
   Size:       2487     750    6418

  Fragments arranged by size: 

                6418    2487     750
 
AlwNI CAG_nnn'CTG

Cuts at:   2611    6267    6662    7398    8970    2611 
   Size:       3656     395     736    1572    3296

  Fragments arranged by size: 

                3656    3296    1572     736     395
 
ApaI G_GGCC'C

Cuts at:   6239    6558    6565    6677    6239 
   Size:        319       7     112    9217

  Fragments arranged by size: 

                9217     319     112       7
 
ApaLI G'TGCA_C

Cuts at:   4940    7712    8868    4940 
   Size:       2772    1156    5727

  Fragments arranged by size: 

                5727    2772    1156
 
ApoI r'AATT_y

Cuts at:   1493    6964    7190    7342    9451    1493 
   Size:       5471     226     152    2109    1697

  Fragments arranged by size: 

                5471    2109    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    8209     160 
   Size:        872    7177    1606

  Fragments arranged by size: 

                7177    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9655
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2126    3428    3990    4777    5076    5306    6289 
   Size:         73     183    1302     562     787     299     230     983

Cuts at:   6289    6557    6980    1870 
   Size:        268     423    4545

  Fragments arranged by size: 

                4545    1302     983     787     562     423     299     268
                 230     183      73
 
AvrII C'CTAG_G

Cuts at:   3004    3004 
   Size:       9655
 
BamHI G'GATC_C

Cuts at:   3147    6985    7352    3147 
   Size:       3838     367    5450

  Fragments arranged by size: 

                5450    3838     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2618    3014    4522    4988    5495 
   Size:        325     217     627     831     396    1508     466     507

Cuts at:   5495    5649    6182    6446    6976    8431     618 
   Size:        154     533     264     530    1455    1842

  Fragments arranged by size: 

                1842    1508    1455     831     627     533     530     507
                 466     396     325     264     217     154
 
BanII G_rGCy'C

Cuts at:   1391    2140    2181    3197    3251    3507    4456    4557    4620 
   Size:        749      41    1016      54     256     949     101      63

Cuts at:   4620    5550    5766    6057    6239    6279    6558    6565    6677 
   Size:        930     216     291     182      40     279       7     112

Cuts at:   6677    6812    6974    1391 
   Size:        135     162    4072

  Fragments arranged by size: 

                4072    1016     949     930     749     291     279     256
                 216     182     162     135     112     101      63      54
                  41      40       7
 
BbeI G_GCGC'C

Cuts at:   2622    4992    6186    6450    2622 
   Size:       2370    1194     264    5827

  Fragments arranged by size: 

                5827    2370    1194     264
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    8684
 
BbvCI CC'TCA_GC

Cuts at:   3951    6616    3951 
   Size:       2665    6990
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2120    2154    3572    3606    7846    7880    1903 
   Size:         34     183      34    1418      34    4240      34    3678

  Fragments arranged by size: 

                4240    3678    1418     183      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   7547    8757    7547 
   Size:       1210    8445
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       9655
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2315    2616    2724 
   Size:        107     122      71     631     605     643     301     108

Cuts at:   2724    3023    3440    3511    4649    6553    6586    6681    8266 
   Size:        299     417      71    1138    1904      33      95    1585

Cuts at:   8266     136 
   Size:       1525

  Fragments arranged by size: 

                1904    1585    1525    1138     643     631     605     417
                 301     299     122     108     107      95      71      71
                  33
 
BglII A'GATC_T

Cuts at:   9650    9650 
   Size:       9655
 
BlpI GC'TnA_GC

Cuts at:   2180    2519    3462    3573    7072    2180 
   Size:        339     943     111    3499    4763

  Fragments arranged by size: 

                4763    3499     943     339     111
 
Bme1580I G_kGCm'C

Cuts at:   1790    4350    4527    4944    5498    5525    6144    6239    6558 
   Size:       2560     177     417     554      27     619      95     319

Cuts at:   6558    6565    6677    7716    8872    1790 
   Size:          7     112    1039    1156    2573

  Fragments arranged by size: 

                2573    2560    1156    1039     619     554     417     319
                 177     112      95      27       7
 
BmgBI CAC'GTC

Cuts at:   1318    2497    3289    6570    1318 
   Size:       1179     792    3281    4403

  Fragments arranged by size: 

                4403    3281    1179     792
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    6051    7386    8344 
   Size:        416      53      83     399      24    4634    1335     958

Cuts at:   8344     442 
   Size:       1753

  Fragments arranged by size: 

                4634    1753    1335     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       9655
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2452    6609    2452 
   Size:       4157    5498
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2129    2276    3475    3792    3815    4298    4487    6062 
   Size:        890     147    1199     317      23     483     189    1575

Cuts at:   6062    6084    6304    6557    8315    1239 
   Size:         22     220     253    1758    2579

  Fragments arranged by size: 

                2579    1758    1575    1199     890     483     317     253
                 220     189     147      23      22
 
Bpu10I CC'TnA_GC

Cuts at:   3010    3951    3957    4127    6616    3010 
   Size:        941       6     170    2489    6049

  Fragments arranged by size: 

                6049    2489     941     170       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2957    3266    4934    5750    7780    8645    8943    2957 
   Size:        309    1668     816    2030     865     298    3669

  Fragments arranged by size: 

                3669    2030    1668     865     816     309     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2558    3824    3927    5858    6510    8318     882 
   Size:        617    1059    1266     103    1931     652    1808    2219

  Fragments arranged by size: 

                2219    1931    1808    1266    1059     652     617     103
            
 
BsaAI yAC'GTr

Cuts at:    493    1204    3700    3892    4834    5355    5581    6952    7446 
   Size:        711    2496     192     942     521     226    1371     494

Cuts at:   7446     493 
   Size:       2702

  Fragments arranged by size: 

                2702    2496    1371     942     711     521     494     226
                 192
 
BsaBI GATnn'nnATC

Cuts at:   9649    9649 
   Size:       9655
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2027    2619    3075    4038 
   Size:         53      83     186     936     494     592     456     963

Cuts at:   4038    4989    5122    5145    5229    6183    6447    6905    7462 
   Size:        951     133      23      84     954     264     458     557

Cuts at:   7462    7844    8479     275 
   Size:        382     635    1451

  Fragments arranged by size: 

                1451     963     954     951     936     635     592     557
                 494     458     456     382     264     186     133      84
                  83      53      23
 
BsaWI w'CCGG_w

Cuts at:   8088    8486    8760    8907    9318    8088 
   Size:        398     274     147     411    8425

  Fragments arranged by size: 

                8425     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    4124    4154    4493    4523    5277 
   Size:         30     165      30    2721      30     339      30     754

Cuts at:   5277    5307    5438    5468    5828    5858    1178 
   Size:         30     131      30     360      30    4975

  Fragments arranged by size: 

                4975    2721     754     360     339     165     131      30
                  30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2768    2822    6059    6081    6287    6761    1186 
   Size:       1582      54    3237      22     206     474    4080

  Fragments arranged by size: 

                4080    3237    1582     474     206      54      22
 
BseYI C'CCAG_C

Cuts at:   2151    2572    2596    3190    3542    4215    5189    5375    6908 
   Size:        421      24     594     352     673     974     186    1533

Cuts at:   6908    8858    9354    2151 
   Size:       1950     496    2452

  Fragments arranged by size: 

                2452    1950    1533     974     673     594     496     421
                 352     186      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2662    5135    5185    5251    5465    1163 
   Size:        573     926    2473      50      66     214    5353

  Fragments arranged by size: 

                5353    2473     926     573     214      66      50
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2048    2278    2353    2952    7105    7866 
   Size:        275     186      47     230      75     599    4153     761

Cuts at:   7866    8894    1540 
   Size:       1028    2301

  Fragments arranged by size: 

                4153    2301    1028     761     599     275     230     186
                  75      47
 
BsiHKAI G_wGCw'C

Cuts at:   2181    2525    2760    4620    4944    7716    7801    8872    2181 
   Size:        344     235    1860     324    2772      85    1071    2964

  Fragments arranged by size: 

                2964    2772    1860    1071     344     324     235      85
            
 
BsmI GAATG_Cn'

Cuts at:   7166    7259    7166 
   Size:         93    9562
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3989    4478    5129    3989 
   Size:        489     651    8515

  Fragments arranged by size: 

                8515     651     489
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2140    2181    2525    2760    3197    3251    3507 
   Size:        399     350      41     344     235     437      54     256

Cuts at:   3507    4350    4456    4527    4557    4620    4944    5498    5525 
   Size:        843     106      71      30      63     324     554      27

Cuts at:   5525    5550    5766    6057    6144    6239    6279    6558    6565 
   Size:         25     216     291      87      95      40     279       7

Cuts at:   6565    6677    6812    6974    7716    7801    8872    1391 
   Size:        112     135     162     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     843     742     554     437     399     350
                 344     324     291     279     256     235     216     162
                 135     112     106      95      87      85      71      63
                  54      41      40      30      27      25       7
 
BspEI T'CCGG_A

Cuts at:   9318    9318 
   Size:       9655
 
BspHI T'CATG_A

Cuts at:   4233    4494    7544    9274    4233 
   Size:        261    3050    1730    4614

  Fragments arranged by size: 

                4614    3050    1730     261
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2446    3102    3249    3852    5064    5155    5207    5267 
   Size:       1602     656     147     603    1212      91      52      60

Cuts at:   5267    5481    5611    5829    6006    6498    7010     844 
   Size:        214     130     218     177     492     512    3489

  Fragments arranged by size: 

                3489    1602    1212     656     603     512     492     218
                 214     177     147     130      91      60      52
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2581    4504    5647    5955    6253    6410    6670 
   Size:        429     758    1923    1143     308     298     157     260

Cuts at:   6670    7542    1394 
   Size:        872    3507

  Fragments arranged by size: 

                3507    1923    1143     872     758     429     308     298
                 260     157
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2839    4036    4795    5305    6868    8150    8324 
   Size:       1183    1591    1197     759     510    1563    1282     174

Cuts at:   8324      65 
   Size:       1396

  Fragments arranged by size: 

                1591    1563    1396    1282    1197    1183     759     510
                 174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2037    2314    2338    3534    4085    5635    6493 
   Size:        110     227     277      24    1196     551    1550     858

Cuts at:   6493    8299    8486    1700 
   Size:       1806     187    2869

  Fragments arranged by size: 

                2869    1806    1550    1196     858     551     277     227
                 187     110      24
 
BsrGI T'GTAC_A

Cuts at:     96    4146    5019      96 
   Size:       4050     873    4732

  Fragments arranged by size: 

                4732    4050     873
 
BssHII G'CGCG_C

Cuts at:   1925    2093    2095    2147    2209    2211    2384    2640    2648 
   Size:        168       2      52      62       2     173     256       8

Cuts at:   2648    6215    1925 
   Size:       3567    5365

  Fragments arranged by size: 

                5365    3567     256     173     168      62      52       8
                   2       2
 
BssSI C'ACGA_G

Cuts at:   4556    5380    7715    8727    4556 
   Size:        824    2335    1012    5484

  Fragments arranged by size: 

                5484    2335    1012     824
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2365    2658    2742    5199     794 
   Size:       1571     293      84    2457    5250

  Fragments arranged by size: 

                5250    2457    1571     293      84
 
BstBI TT'CG_AA

Cuts at:   2007    8459    2007 
   Size:       6452    3203
 
BstEII G'GTnAC_C

Cuts at:   1184    3082    3127    5816    1184 
   Size:       1898      45    2689    5023

  Fragments arranged by size: 

                5023    2689    1898      45
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3139    3716    3864    5068    5884    5908    5926    6724    6863 
   Size:        577     148    1204     816      24      18     798     139

Cuts at:   6863    3139 
   Size:       5931

  Fragments arranged by size: 

                5931    1204     816     798     577     148     139      24
                  18
 
BstYI r'GATC_y

Cuts at:   1972    3147    3638    6985    7352    7737    7754    9206    9292 
   Size:       1175     491    3347     367     385      17    1452      86

Cuts at:   9292    9304    9650    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                3347    1977    1452    1175     491     385     367     346
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3825    6425    3825 
   Size:       2600    7055
 
BtgI C'CryG_G

Cuts at:    513    1065    2022    2548    2763    3594    4119    4518    4746 
   Size:        552     957     526     215     831     525     399     228

Cuts at:   4746    5400    5499    5868    5925    6117    6333    6441    6581 
   Size:        654      99     369      57     192     216     108     140

Cuts at:   6581    6717     513 
   Size:        136    3451

  Fragments arranged by size: 

                3451     957     831     654     552     526     525     399
                 369     228     216     215     192     140     136     108
                  99      57
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    5122    6042     487 
   Size:        683      56    3896     920    4100

  Fragments arranged by size: 

                4100    3896     920     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2138    4013    5249    6693    7171    7965    7985    2138 
   Size:       1875    1236    1444     478     794      20    3808

  Fragments arranged by size: 

                3808    1875    1444    1236     794     478      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       9655
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    6556    6591     529 
   Size:         35    5992      35    3593

  Fragments arranged by size: 

                5992    3593      35      35
 
DraI TTT'AAA

Cuts at:   6959    6959 
   Size:       9655
 
DraIII CAC_nnn'GTG

Cuts at:   2365    4378    4834    4912    5584    2365 
   Size:       2013     456      78     672    6436

  Fragments arranged by size: 

                6436    2013     672     456      78
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3080    4799    4886    8662     809 
   Size:        507    1764    1719      87    3776    1802

  Fragments arranged by size: 

                3776    1802    1764    1719     507      87
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2045    2275    2316 
   Size:         54    1425      50     275      18     215     230      41

Cuts at:   2316    2343    2460    2559    2949    3610    3765    4239    4567 
   Size:         27     117      99     390     661     155     474     328

Cuts at:   4567    4650    5397    5633    5781    5871    5895    5928    6120 
   Size:         83     747     236     148      90      24      33     192

Cuts at:   6120    7102    7991    9620       8 
   Size:        982     889    1629      43

  Fragments arranged by size: 

                1629    1425     982     889     747     661     474     390
                 328     275     236     230     215     192     155     148
                 117      99      90      83      54      50      43      41
                  33      27      24      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2045    2275    2949    7102    1537 
   Size:        275     233     230     674    4153    4090

  Fragments arranged by size: 

                4153    4090     674     275     233     230
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2745    3527    3917    4733    5202    7585    1353 
   Size:       1392     782     390     816     469    2383    3423

  Fragments arranged by size: 

                3423    2383    1392     816     782     469     390
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    6463    8242    8616    8762     387 
   Size:       1064     138    4874    1779     374     146    1280

  Fragments arranged by size: 

                4874    1779    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2179    4618    2179 
   Size:       2439    7216
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2129    2170    2276    2588    3475    3508    3792 
   Size:        393     497      41     106     312     887      33     284

Cuts at:   3792    3815    4022    4298    4487    5226    5392    6062    6084 
   Size:         23     207     276     189     739     166     670      22

Cuts at:   6084    6304    6509    6557    7718    8315    9102    9370    1239 
   Size:        220     205      48    1161     597     787     268    1524

  Fragments arranged by size: 

                1524    1161     887     787     739     670     597     497
                 393     312     284     276     268     220     207     205
                 189     166     106      48      41      33      23      22
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3335    3950    3335 
   Size:        615    9040
 
EcoO109I rG'GnC_Cy

Cuts at:   2608    3025    3346    3907    3943    4325    4927    5288    6037 
   Size:        417     321     561      36     382     602     361     749

Cuts at:   6037    6235    6554    6561    6562    6588    6659    6673    6886 
   Size:        198     319       7       1      26      71      14     213

Cuts at:   6886    2608 
   Size:       5377

  Fragments arranged by size: 

                5377     749     602     561     417     382     361     321
                 319     213     198      71      36      26      14       7
                   1
 
EcoRI G'AATT_C

Cuts at:   6964    7342    6964 
   Size:        378    9277
 
FspI TGC'GCA

Cuts at:   2195    8161    2195 
   Size:       5966    3689
 
HaeII r_GCGC'y

Cuts at:   1678    2087    2399    2513    2622    3446    3462    4992    5342 
   Size:        409     312     114     109     824      16    1530     350

Cuts at:   5342    5430    5454    5780    6186    6450    6755    8802    1678 
   Size:         88      24     326     406     264     305    2047    2531

  Fragments arranged by size: 

                2531    2047    1530     824     409     406     350     326
                 312     305     264     114     109      88      24      16
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3623    3655    4181    4213    5277 
   Size:         32     135      32    2051      32     526      32    1064

Cuts at:   5277    5309    5328    5360    5374    5406    5438    5470    5800 
   Size:         32      19      32      14      32      32      32     330

Cuts at:   5800    5828    5832    5860    6794    6826    8296    8328    8370 
   Size:         28       4      28     934      32    1470      32      42

Cuts at:   8370    8402    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2051    1470    1064     934     526     330     135
                  42      32      32      32      32      32      32      32
                  32      32      32      32      32      28      28      19
                  14       4
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2962    4312    5641    6999    7245     669 
   Size:        988     281    1024    1350    1329    1358     246    3079

  Fragments arranged by size: 

                3079    1358    1350    1329    1024     988     281     246
            
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       9655
 
HpaI GTT'AAC

Cuts at:   7245    7245 
   Size:       9655
 
KasI G'GCGC_C

Cuts at:   2618    4988    6182    6446    2618 
   Size:       2370    1194     264    5827

  Fragments arranged by size: 

                5827    2370    1194     264
 
KpnI G_GTAC'C

Cuts at:   5653    6980    5653 
   Size:       1327    8328
 
MfeI C'AATT_G

Cuts at:   7254    7254 
   Size:       9655
 
MluI A'CGCG_T

Cuts at:   9531    9531 
   Size:       9655
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4554    8769    8953    1305 
   Size:       3249    4215     184    2007

  Fragments arranged by size: 

                4215    3249    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2462    2561    3767    4241    4569 
   Size:         54    1425     343     630      99    1206     474     328

Cuts at:   4569    5897    5930    6122      10 
   Size:       1328      33     192    3543

  Fragments arranged by size: 

                3543    1425    1328    1206     630     474     343     328
                 192      99      54      33
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2339    3137    3737    4148    4548    4983    5384 
   Size:        648    1173     798     600     411     400     435     401

Cuts at:   5384    5906    5924    6176    6722    6728    7613    7972    8131 
   Size:        522      18     252     546       6     885     359     159

Cuts at:   8131     518 
   Size:       2042

  Fragments arranged by size: 

                2042    1173     885     798     648     600     546     522
                 435     411     401     400     359     252     159      18
                   6
 
MspA1I CmG'CkG

Cuts at:   1606    2062    2172    2329    2355    2550    2635    3236    3384 
   Size:        456     110     157      26     195      85     601     148

Cuts at:   3384    3453    3577    4219    4411    5422    5479    5631    5668 
   Size:         69     124     642     192    1011      57     152      37

Cuts at:   5668    6583    7071    7748    8896    9141    1606 
   Size:        915     488     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1011     915     677     642     601     488
                 456     245     195     192     157     152     148     124
                 110      85      69      57      37      26
 
NaeI GCC'GGC

Cuts at:   2316    3536    6495    2316 
   Size:       1220    2959    5476

  Fragments arranged by size: 

                5476    2959    1220
 
NarI GG'CG_CC

Cuts at:   2619    4989    6183    6447    2619 
   Size:       2370    1194     264    5827

  Fragments arranged by size: 

                5827    2370    1194     264
 
NcoI C'CATG_G

Cuts at:    513    1065    2022    2763    3594    5925     513 
   Size:        552     957     741     831    2331    4243

  Fragments arranged by size: 

                4243    2331     957     831     741     552
 
NdeI CA'TA_TG

Cuts at:    387    4458     387 
   Size:       4071    5584
 
NgoMIV G'CCGG_C

Cuts at:   2314    3534    6493    2314 
   Size:       1220    2959    5476

  Fragments arranged by size: 

                5476    2959    1220
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       9655
 
NotI GC'GGCC_GC

Cuts at:   7102    7102 
   Size:       9655
 
NspI r_CATG'y

Cuts at:   1132    1229    7013    8558    1132 
   Size:         97    5784    1545    2229

  Fragments arranged by size: 

                5784    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    6452    1348 
   Size:       5104    4551
 
PciI A'CATG_T

Cuts at:   8554    8554 
   Size:       9655
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       9655
 
PfoI T'CCnGG_A

Cuts at:   2030    2253    6699    2030 
   Size:        223    4446    4986

  Fragments arranged by size: 

                4986    4446     223
 
PmeI GTTT'AAAC

Cuts at:   6959    6959 
   Size:       9655
 
PmlI CAC'GTG

Cuts at:   3700    5355    5581    3700 
   Size:       1655     226    7774

  Fragments arranged by size: 

                7774    1655     226
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   7724    7756    9237    9269    7724 
   Size:         32    1481      32    8110

  Fragments arranged by size: 

                8110    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3346    3907    4927    6037    3346 
   Size:        561    1020    1110    6964

  Fragments arranged by size: 

                6964    1110    1020     561
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       9655
 
PsiI TTA'TAA

Cuts at:   7225    7225 
   Size:       9655
 
PspOMI G'GGCC_C

Cuts at:   6235    6554    6561    6673    6235 
   Size:        319       7     112    9217

  Fragments arranged by size: 

                9217     319     112       7
 
PstI C_TGCA'G

Cuts at:    830    2328    2717    2747    3366    4410    5478    5622    7007 
   Size:       1498     389      30     619    1044    1068     144    1385

Cuts at:   7007     830 
   Size:       3478

  Fragments arranged by size: 

                3478    1498    1385    1068    1044     619     389     144
                  30
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       9655
 
PvuII CAG'CTG

Cuts at:   2329    3236    4411    5479    5631    5668    2329 
   Size:        907    1175    1068     152      37    6316

  Fragments arranged by size: 

                6316    1175    1068     907     152      37
 
RsrII CG'GwC_CG

Cuts at:   6376    6376 
   Size:       9655
 
SacI G_AGCT'C

Cuts at:   2181    4620    2181 
   Size:       2439    7216
 
SacII CC_GC'GG

Cuts at:   2551    6584    2551 
   Size:       4033    5622
 
SalI G'TCGA_C

Cuts at:   1936    6997    1936 
   Size:       5061    4594
 
SanDI GG'GwC_CC

Cuts at:   3346    4927    3346 
   Size:       1581    8074
 
SapI GCTCTTCn'nnn_

Cuts at:   3527    3527 
   Size:       9655
 
SbfI CC_TGCA'GG

Cuts at:   5622    7007    5622 
   Size:       1385    8270
 
ScaI AGT'ACT

Cuts at:   6061    7903    9615    6061 
   Size:       1842    1712    6101

  Fragments arranged by size: 

                6101    1842    1712
 
SexAI A'CCwGG_T

Cuts at:   6816    6816 
   Size:       9655
 
SfcI C'TryA_G

Cuts at:    826    1045    2324    2713    2743    3362    4406    5474    5618 
   Size:        219    1279     389      30     619    1044    1068     144

Cuts at:   5618    7003    8138    8819    9010     826 
   Size:       1385    1135     681     191    1471

  Fragments arranged by size: 

                1471    1385    1279    1135    1068    1044     681     619
                 389     219     191     144      30
 
SfoI GGC'GCC

Cuts at:   2620    4990    6184    6448    2620 
   Size:       2370    1194     264    5827

  Fragments arranged by size: 

                5827    2370    1194     264
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9655
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    2338    1700 
   Size:        638    9017
 
SmaI CCC'GGG

Cuts at:   1872    2128    3992    5078    5308    6559    6982    1872 
   Size:        256    1864    1086     230    1251     423    4545

  Fragments arranged by size: 

                4545    1864    1251    1086     423     256     230
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2972    3281    4949    5765    7759    8660 
   Size:        197     926    1029     309    1668     816    1994     901

Cuts at:   8660    8922     820 
   Size:        262    1553

  Fragments arranged by size: 

                1994    1668    1553    1029     926     901     816     309
                 262     197
 
SnaBI TAC'GTA

Cuts at:    493    6952    7446     493 
   Size:       6459     494    2702

  Fragments arranged by size: 

                6459    2702     494
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       9655
 
SphI G_CATG'C

Cuts at:   1132    7013    1132 
   Size:       5881    3774
 
SspI AAT'ATT

Cuts at:      5      52    6078    7579       5 
   Size:         47    6026    1501    2081

  Fragments arranged by size: 

                6026    2081    1501      47
 
StuI AGG'CCT

Cuts at:   1517    3434    6287    1517 
   Size:       1917    2853    4885

  Fragments arranged by size: 

                4885    2853    1917
 
StyI C'CwwG_G

Cuts at:    513    1065    2022    2763    3004    3594    5461    5925    6549 
   Size:        552     957     741     241     590    1867     464     624

Cuts at:   6549    7094     513 
   Size:        545    3074

  Fragments arranged by size: 

                3074    1867     957     741     624     590     552     545
                 464     241
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    5429    5996    6203    7682    7699    7852    8037 
   Size:       1255    3849     567     207    1479      17     153     185

Cuts at:   8037    8482     325 
   Size:        445    1498

  Fragments arranged by size: 

                3849    1498    1479    1255     567     445     207     185
                 153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4146    5019    6059 
   Size:        274      80      33      51    1443    2169     873    1040

Cuts at:   6059    7901    9613      96 
   Size:       1842    1712     138

  Fragments arranged by size: 

                2169    1842    1712    1443    1040     873     274     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2886    2918    6346    6378    9615    9647    2886 
   Size:         32    3428      32    3237      32    2894

  Fragments arranged by size: 

                3428    3237    2894      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2957    3290    1316 
   Size:       1641     333    7681

  Fragments arranged by size: 

                7681    1641     333
 
XbaI T'CTAG_A

Cuts at:   6991    6991 
   Size:       9655
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2326    2569    3601    4592    5383    5620    5881    5905    6364 
   Size:        243    1032     991     791     237     261      24     459

Cuts at:   6364    6724    2326 
   Size:        360    5257

  Fragments arranged by size: 

                5257    1032     991     791     459     360     261     243
                 237      24
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       9655
 
XmaI C'CCGG_G

Cuts at:   1870    2126    3990    5076    5306    6557    6980    1870 
   Size:        256    1864    1086     230    1251     423    4545

  Fragments arranged by size: 

                4545    1864    1251    1086     423     256     230
 
XmnI GAAnn'nnTTC

Cuts at:   4061    5045    7784    4061 
   Size:        984    2739    5932

  Fragments arranged by size: 

                5932    2739     984
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3076    5123    7463     276 
   Size:         53      83     186     936    1542    2047    2340    2468

  Fragments arranged by size: 

                2468    2340    2047    1542     936     186      83      53
            
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     AscI     BaeI    BfrBI    BsiWI  BstZ17I    EcoRV     FalI
     FseI    FspAI     NruI     NsiI     PacI    PspXI     PsrI     SfiI
     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com