(Circular) (Six-base) MAPSORT of: pFN21ASDA0921.seq Check: 5602 from: 1 to: 9655 pFN21ASDA0921 9655 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2446 5155 5207 5267 5611 5829 2446 Size: 2709 52 60 344 218 6272 Fragments arranged by size: 6272 2709 344 218 60 52 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3078 5125 7465 278 Size: 53 83 186 936 1542 2047 2340 2468 Fragments arranged by size: 2468 2340 2047 1542 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6948 6998 1937 Size: 5011 50 4594 Fragments arranged by size: 5011 4594 50 Acc65I G'GTAC_C Cuts at: 5649 6976 5649 Size: 1327 8328 AclI AA'CG_TT Cuts at: 1563 7782 8155 1563 Size: 6219 373 3063 Fragments arranged by size: 6219 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2170 2588 3508 4022 5226 5392 6509 7718 Size: 538 418 920 514 1204 166 1117 1209 Cuts at: 7718 9102 9370 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1209 1204 1117 920 538 514 418 268 166 AfeI AGC'GCT Cuts at: 3460 5340 5428 3460 Size: 1880 88 7687 Fragments arranged by size: 7687 1880 88 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9458 AflIII A'CryG_T Cuts at: 1203 4833 5352 8554 9531 1203 Size: 3630 519 3202 977 1327 Fragments arranged by size: 3630 3202 1327 977 519 AgeI A'CCGG_T Cuts at: 8486 8486 Size: 9655 AhdI GACnn_n'nnGTC Cuts at: 5814 8384 5814 Size: 2570 7085 AleI CACnn'nnGTG Cuts at: 1166 2339 6176 1166 Size: 1173 3837 4645 Fragments arranged by size: 4645 3837 1173 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3671 4421 1184 Size: 2487 750 6418 Fragments arranged by size: 6418 2487 750 AlwNI CAG_nnn'CTG Cuts at: 2611 6267 6662 7398 8970 2611 Size: 3656 395 736 1572 3296 Fragments arranged by size: 3656 3296 1572 736 395 ApaI G_GGCC'C Cuts at: 6239 6558 6565 6677 6239 Size: 319 7 112 9217 Fragments arranged by size: 9217 319 112 7 ApaLI G'TGCA_C Cuts at: 4940 7712 8868 4940 Size: 2772 1156 5727 Fragments arranged by size: 5727 2772 1156 ApoI r'AATT_y Cuts at: 1493 6964 7190 7342 9451 1493 Size: 5471 226 152 2109 1697 Fragments arranged by size: 5471 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 8209 160 Size: 872 7177 1606 Fragments arranged by size: 7177 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9655 AvaI C'yCGr_G Cuts at: 1870 1943 2126 3428 3990 4777 5076 5306 6289 Size: 73 183 1302 562 787 299 230 983 Cuts at: 6289 6557 6980 1870 Size: 268 423 4545 Fragments arranged by size: 4545 1302 983 787 562 423 299 268 230 183 73 AvrII C'CTAG_G Cuts at: 3004 3004 Size: 9655 BamHI G'GATC_C Cuts at: 3147 6985 7352 3147 Size: 3838 367 5450 Fragments arranged by size: 5450 3838 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2618 3014 4522 4988 5495 Size: 325 217 627 831 396 1508 466 507 Cuts at: 5495 5649 6182 6446 6976 8431 618 Size: 154 533 264 530 1455 1842 Fragments arranged by size: 1842 1508 1455 831 627 533 530 507 466 396 325 264 217 154 BanII G_rGCy'C Cuts at: 1391 2140 2181 3197 3251 3507 4456 4557 4620 Size: 749 41 1016 54 256 949 101 63 Cuts at: 4620 5550 5766 6057 6239 6279 6558 6565 6677 Size: 930 216 291 182 40 279 7 112 Cuts at: 6677 6812 6974 1391 Size: 135 162 4072 Fragments arranged by size: 4072 1016 949 930 749 291 279 256 216 182 162 135 112 101 63 54 41 40 7 BbeI G_GCGC'C Cuts at: 2622 4992 6186 6450 2622 Size: 2370 1194 264 5827 Fragments arranged by size: 5827 2370 1194 264 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 8684 BbvCI CC'TCA_GC Cuts at: 3951 6616 3951 Size: 2665 6990 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2120 2154 3572 3606 7846 7880 1903 Size: 34 183 34 1418 34 4240 34 3678 Fragments arranged by size: 4240 3678 1418 183 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7547 8757 7547 Size: 1210 8445 BclI T'GATC_A Cuts at: 1548 1548 Size: 9655 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2315 2616 2724 Size: 107 122 71 631 605 643 301 108 Cuts at: 2724 3023 3440 3511 4649 6553 6586 6681 8266 Size: 299 417 71 1138 1904 33 95 1585 Cuts at: 8266 136 Size: 1525 Fragments arranged by size: 1904 1585 1525 1138 643 631 605 417 301 299 122 108 107 95 71 71 33 BglII A'GATC_T Cuts at: 9650 9650 Size: 9655 BlpI GC'TnA_GC Cuts at: 2180 2519 3462 3573 7072 2180 Size: 339 943 111 3499 4763 Fragments arranged by size: 4763 3499 943 339 111 Bme1580I G_kGCm'C Cuts at: 1790 4350 4527 4944 5498 5525 6144 6239 6558 Size: 2560 177 417 554 27 619 95 319 Cuts at: 6558 6565 6677 7716 8872 1790 Size: 7 112 1039 1156 2573 Fragments arranged by size: 2573 2560 1156 1039 619 554 417 319 177 112 95 27 7 BmgBI CAC'GTC Cuts at: 1318 2497 3289 6570 1318 Size: 1179 792 3281 4403 Fragments arranged by size: 4403 3281 1179 792 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6051 7386 8344 Size: 416 53 83 399 24 4634 1335 958 Cuts at: 8344 442 Size: 1753 Fragments arranged by size: 4634 1753 1335 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9655 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2452 6609 2452 Size: 4157 5498 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2276 3475 3792 3815 4298 4487 6062 Size: 890 147 1199 317 23 483 189 1575 Cuts at: 6062 6084 6304 6557 8315 1239 Size: 22 220 253 1758 2579 Fragments arranged by size: 2579 1758 1575 1199 890 483 317 253 220 189 147 23 22 Bpu10I CC'TnA_GC Cuts at: 3010 3951 3957 4127 6616 3010 Size: 941 6 170 2489 6049 Fragments arranged by size: 6049 2489 941 170 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2957 3266 4934 5750 7780 8645 8943 2957 Size: 309 1668 816 2030 865 298 3669 Fragments arranged by size: 3669 2030 1668 865 816 309 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2558 3824 3927 5858 6510 8318 882 Size: 617 1059 1266 103 1931 652 1808 2219 Fragments arranged by size: 2219 1931 1808 1266 1059 652 617 103 BsaAI yAC'GTr Cuts at: 493 1204 3700 3892 4834 5355 5581 6952 7446 Size: 711 2496 192 942 521 226 1371 494 Cuts at: 7446 493 Size: 2702 Fragments arranged by size: 2702 2496 1371 942 711 521 494 226 192 BsaBI GATnn'nnATC Cuts at: 9649 9649 Size: 9655 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 2619 3075 4038 Size: 53 83 186 936 494 592 456 963 Cuts at: 4038 4989 5122 5145 5229 6183 6447 6905 7462 Size: 951 133 23 84 954 264 458 557 Cuts at: 7462 7844 8479 275 Size: 382 635 1451 Fragments arranged by size: 1451 963 954 951 936 635 592 557 494 458 456 382 264 186 133 84 83 53 23 BsaWI w'CCGG_w Cuts at: 8088 8486 8760 8907 9318 8088 Size: 398 274 147 411 8425 Fragments arranged by size: 8425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4124 4154 4493 4523 5277 Size: 30 165 30 2721 30 339 30 754 Cuts at: 5277 5307 5438 5468 5828 5858 1178 Size: 30 131 30 360 30 4975 Fragments arranged by size: 4975 2721 754 360 339 165 131 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2768 2822 6059 6081 6287 6761 1186 Size: 1582 54 3237 22 206 474 4080 Fragments arranged by size: 4080 3237 1582 474 206 54 22 BseYI C'CCAG_C Cuts at: 2151 2572 2596 3190 3542 4215 5189 5375 6908 Size: 421 24 594 352 673 974 186 1533 Cuts at: 6908 8858 9354 2151 Size: 1950 496 2452 Fragments arranged by size: 2452 1950 1533 974 673 594 496 421 352 186 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2662 5135 5185 5251 5465 1163 Size: 573 926 2473 50 66 214 5353 Fragments arranged by size: 5353 2473 926 573 214 66 50 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2048 2278 2353 2952 7105 7866 Size: 275 186 47 230 75 599 4153 761 Cuts at: 7866 8894 1540 Size: 1028 2301 Fragments arranged by size: 4153 2301 1028 761 599 275 230 186 75 47 BsiHKAI G_wGCw'C Cuts at: 2181 2525 2760 4620 4944 7716 7801 8872 2181 Size: 344 235 1860 324 2772 85 1071 2964 Fragments arranged by size: 2964 2772 1860 1071 344 324 235 85 BsmI GAATG_Cn' Cuts at: 7166 7259 7166 Size: 93 9562 BsmBI CGTCTCn'nnnn_ Cuts at: 3989 4478 5129 3989 Size: 489 651 8515 Fragments arranged by size: 8515 651 489 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2181 2525 2760 3197 3251 3507 Size: 399 350 41 344 235 437 54 256 Cuts at: 3507 4350 4456 4527 4557 4620 4944 5498 5525 Size: 843 106 71 30 63 324 554 27 Cuts at: 5525 5550 5766 6057 6144 6239 6279 6558 6565 Size: 25 216 291 87 95 40 279 7 Cuts at: 6565 6677 6812 6974 7716 7801 8872 1391 Size: 112 135 162 742 85 1071 2174 Fragments arranged by size: 2174 1071 843 742 554 437 399 350 344 324 291 279 256 235 216 162 135 112 106 95 87 85 71 63 54 41 40 30 27 25 7 BspEI T'CCGG_A Cuts at: 9318 9318 Size: 9655 BspHI T'CATG_A Cuts at: 4233 4494 7544 9274 4233 Size: 261 3050 1730 4614 Fragments arranged by size: 4614 3050 1730 261 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2446 3102 3249 3852 5064 5155 5207 5267 Size: 1602 656 147 603 1212 91 52 60 Cuts at: 5267 5481 5611 5829 6006 6498 7010 844 Size: 214 130 218 177 492 512 3489 Fragments arranged by size: 3489 1602 1212 656 603 512 492 218 214 177 147 130 91 60 52 BsrBI CCG'CTC Cuts at: 1394 1823 2581 4504 5647 5955 6253 6410 6670 Size: 429 758 1923 1143 308 298 157 260 Cuts at: 6670 7542 1394 Size: 872 3507 Fragments arranged by size: 3507 1923 1143 872 758 429 308 298 260 157 BsrDI GCAATG_nn' Cuts at: 65 1248 2839 4036 4795 5305 6868 8150 8324 Size: 1183 1591 1197 759 510 1563 1282 174 Cuts at: 8324 65 Size: 1396 Fragments arranged by size: 1591 1563 1396 1282 1197 1183 759 510 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 2314 2338 3534 4085 5635 6493 Size: 110 227 277 24 1196 551 1550 858 Cuts at: 6493 8299 8486 1700 Size: 1806 187 2869 Fragments arranged by size: 2869 1806 1550 1196 858 551 277 227 187 110 24 BsrGI T'GTAC_A Cuts at: 96 4146 5019 96 Size: 4050 873 4732 Fragments arranged by size: 4732 4050 873 BssHII G'CGCG_C Cuts at: 1925 2093 2095 2147 2209 2211 2384 2640 2648 Size: 168 2 52 62 2 173 256 8 Cuts at: 2648 6215 1925 Size: 3567 5365 Fragments arranged by size: 5365 3567 256 173 168 62 52 8 2 2 BssSI C'ACGA_G Cuts at: 4556 5380 7715 8727 4556 Size: 824 2335 1012 5484 Fragments arranged by size: 5484 2335 1012 824 BstAPI GCAn_nnn'nTGC Cuts at: 794 2365 2658 2742 5199 794 Size: 1571 293 84 2457 5250 Fragments arranged by size: 5250 2457 1571 293 84 BstBI TT'CG_AA Cuts at: 2007 8459 2007 Size: 6452 3203 BstEII G'GTnAC_C Cuts at: 1184 3082 3127 5816 1184 Size: 1898 45 2689 5023 Fragments arranged by size: 5023 2689 1898 45 BstXI CCAn_nnnn'nTGG Cuts at: 3139 3716 3864 5068 5884 5908 5926 6724 6863 Size: 577 148 1204 816 24 18 798 139 Cuts at: 6863 3139 Size: 5931 Fragments arranged by size: 5931 1204 816 798 577 148 139 24 18 BstYI r'GATC_y Cuts at: 1972 3147 3638 6985 7352 7737 7754 9206 9292 Size: 1175 491 3347 367 385 17 1452 86 Cuts at: 9292 9304 9650 1972 Size: 12 346 1977 Fragments arranged by size: 3347 1977 1452 1175 491 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3825 6425 3825 Size: 2600 7055 BtgI C'CryG_G Cuts at: 513 1065 2022 2548 2763 3594 4119 4518 4746 Size: 552 957 526 215 831 525 399 228 Cuts at: 4746 5400 5499 5868 5925 6117 6333 6441 6581 Size: 654 99 369 57 192 216 108 140 Cuts at: 6581 6717 513 Size: 136 3451 Fragments arranged by size: 3451 957 831 654 552 526 525 399 369 228 216 215 192 140 136 108 99 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5122 6042 487 Size: 683 56 3896 920 4100 Fragments arranged by size: 4100 3896 920 683 56 BtsI GCAGTG_nn' Cuts at: 2138 4013 5249 6693 7171 7965 7985 2138 Size: 1875 1236 1444 478 794 20 3808 Fragments arranged by size: 3808 1875 1444 1236 794 478 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9655 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6556 6591 529 Size: 35 5992 35 3593 Fragments arranged by size: 5992 3593 35 35 DraI TTT'AAA Cuts at: 6959 6959 Size: 9655 DraIII CAC_nnn'GTG Cuts at: 2365 4378 4834 4912 5584 2365 Size: 2013 456 78 672 6436 Fragments arranged by size: 6436 2013 672 456 78 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3080 4799 4886 8662 809 Size: 507 1764 1719 87 3776 1802 Fragments arranged by size: 3776 1802 1764 1719 507 87 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 2275 2316 Size: 54 1425 50 275 18 215 230 41 Cuts at: 2316 2343 2460 2559 2949 3610 3765 4239 4567 Size: 27 117 99 390 661 155 474 328 Cuts at: 4567 4650 5397 5633 5781 5871 5895 5928 6120 Size: 83 747 236 148 90 24 33 192 Cuts at: 6120 7102 7991 9620 8 Size: 982 889 1629 43 Fragments arranged by size: 1629 1425 982 889 747 661 474 390 328 275 236 230 215 192 155 148 117 99 90 83 54 50 43 41 33 27 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2045 2275 2949 7102 1537 Size: 275 233 230 674 4153 4090 Fragments arranged by size: 4153 4090 674 275 233 230 EarI CTCTTCn'nnn_ Cuts at: 1353 2745 3527 3917 4733 5202 7585 1353 Size: 1392 782 390 816 469 2383 3423 Fragments arranged by size: 3423 2383 1392 816 782 469 390 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6463 8242 8616 8762 387 Size: 1064 138 4874 1779 374 146 1280 Fragments arranged by size: 4874 1779 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2179 4618 2179 Size: 2439 7216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2170 2276 2588 3475 3508 3792 Size: 393 497 41 106 312 887 33 284 Cuts at: 3792 3815 4022 4298 4487 5226 5392 6062 6084 Size: 23 207 276 189 739 166 670 22 Cuts at: 6084 6304 6509 6557 7718 8315 9102 9370 1239 Size: 220 205 48 1161 597 787 268 1524 Fragments arranged by size: 1524 1161 887 787 739 670 597 497 393 312 284 276 268 220 207 205 189 166 106 48 41 33 23 22 EcoNI CCTnn'n_nnAGG Cuts at: 3335 3950 3335 Size: 615 9040 EcoO109I rG'GnC_Cy Cuts at: 2608 3025 3346 3907 3943 4325 4927 5288 6037 Size: 417 321 561 36 382 602 361 749 Cuts at: 6037 6235 6554 6561 6562 6588 6659 6673 6886 Size: 198 319 7 1 26 71 14 213 Cuts at: 6886 2608 Size: 5377 Fragments arranged by size: 5377 749 602 561 417 382 361 321 319 213 198 71 36 26 14 7 1 EcoRI G'AATT_C Cuts at: 6964 7342 6964 Size: 378 9277 FspI TGC'GCA Cuts at: 2195 8161 2195 Size: 5966 3689 HaeII r_GCGC'y Cuts at: 1678 2087 2399 2513 2622 3446 3462 4992 5342 Size: 409 312 114 109 824 16 1530 350 Cuts at: 5342 5430 5454 5780 6186 6450 6755 8802 1678 Size: 88 24 326 406 264 305 2047 2531 Fragments arranged by size: 2531 2047 1530 824 409 406 350 326 312 305 264 114 109 88 24 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3623 3655 4181 4213 5277 Size: 32 135 32 2051 32 526 32 1064 Cuts at: 5277 5309 5328 5360 5374 5406 5438 5470 5800 Size: 32 19 32 14 32 32 32 330 Cuts at: 5800 5828 5832 5860 6794 6826 8296 8328 8370 Size: 28 4 28 934 32 1470 32 42 Cuts at: 8370 8402 1373 Size: 32 2626 Fragments arranged by size: 2626 2051 1470 1064 934 526 330 135 42 32 32 32 32 32 32 32 32 32 32 32 32 28 28 19 14 4 HincII GTy'rAC Cuts at: 669 1657 1938 2962 4312 5641 6999 7245 669 Size: 988 281 1024 1350 1329 1358 246 3079 Fragments arranged by size: 3079 1358 1350 1329 1024 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9655 HpaI GTT'AAC Cuts at: 7245 7245 Size: 9655 KasI G'GCGC_C Cuts at: 2618 4988 6182 6446 2618 Size: 2370 1194 264 5827 Fragments arranged by size: 5827 2370 1194 264 KpnI G_GTAC'C Cuts at: 5653 6980 5653 Size: 1327 8328 MfeI C'AATT_G Cuts at: 7254 7254 Size: 9655 MluI A'CGCG_T Cuts at: 9531 9531 Size: 9655 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4554 8769 8953 1305 Size: 3249 4215 184 2007 Fragments arranged by size: 4215 3249 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2462 2561 3767 4241 4569 Size: 54 1425 343 630 99 1206 474 328 Cuts at: 4569 5897 5930 6122 10 Size: 1328 33 192 3543 Fragments arranged by size: 3543 1425 1328 1206 630 474 343 328 192 99 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 2339 3137 3737 4148 4548 4983 5384 Size: 648 1173 798 600 411 400 435 401 Cuts at: 5384 5906 5924 6176 6722 6728 7613 7972 8131 Size: 522 18 252 546 6 885 359 159 Cuts at: 8131 518 Size: 2042 Fragments arranged by size: 2042 1173 885 798 648 600 546 522 435 411 401 400 359 252 159 18 6 MspA1I CmG'CkG Cuts at: 1606 2062 2172 2329 2355 2550 2635 3236 3384 Size: 456 110 157 26 195 85 601 148 Cuts at: 3384 3453 3577 4219 4411 5422 5479 5631 5668 Size: 69 124 642 192 1011 57 152 37 Cuts at: 5668 6583 7071 7748 8896 9141 1606 Size: 915 488 677 1148 245 2120 Fragments arranged by size: 2120 1148 1011 915 677 642 601 488 456 245 195 192 157 152 148 124 110 85 69 57 37 26 NaeI GCC'GGC Cuts at: 2316 3536 6495 2316 Size: 1220 2959 5476 Fragments arranged by size: 5476 2959 1220 NarI GG'CG_CC Cuts at: 2619 4989 6183 6447 2619 Size: 2370 1194 264 5827 Fragments arranged by size: 5827 2370 1194 264 NcoI C'CATG_G Cuts at: 513 1065 2022 2763 3594 5925 513 Size: 552 957 741 831 2331 4243 Fragments arranged by size: 4243 2331 957 831 741 552 NdeI CA'TA_TG Cuts at: 387 4458 387 Size: 4071 5584 NgoMIV G'CCGG_C Cuts at: 2314 3534 6493 2314 Size: 1220 2959 5476 Fragments arranged by size: 5476 2959 1220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9655 NotI GC'GGCC_GC Cuts at: 7102 7102 Size: 9655 NspI r_CATG'y Cuts at: 1132 1229 7013 8558 1132 Size: 97 5784 1545 2229 Fragments arranged by size: 5784 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 6452 1348 Size: 5104 4551 PciI A'CATG_T Cuts at: 8554 8554 Size: 9655 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9655 PfoI T'CCnGG_A Cuts at: 2030 2253 6699 2030 Size: 223 4446 4986 Fragments arranged by size: 4986 4446 223 PmeI GTTT'AAAC Cuts at: 6959 6959 Size: 9655 PmlI CAC'GTG Cuts at: 3700 5355 5581 3700 Size: 1655 226 7774 Fragments arranged by size: 7774 1655 226 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7724 7756 9237 9269 7724 Size: 32 1481 32 8110 Fragments arranged by size: 8110 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3346 3907 4927 6037 3346 Size: 561 1020 1110 6964 Fragments arranged by size: 6964 1110 1020 561 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9655 PsiI TTA'TAA Cuts at: 7225 7225 Size: 9655 PspOMI G'GGCC_C Cuts at: 6235 6554 6561 6673 6235 Size: 319 7 112 9217 Fragments arranged by size: 9217 319 112 7 PstI C_TGCA'G Cuts at: 830 2328 2717 2747 3366 4410 5478 5622 7007 Size: 1498 389 30 619 1044 1068 144 1385 Cuts at: 7007 830 Size: 3478 Fragments arranged by size: 3478 1498 1385 1068 1044 619 389 144 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9655 PvuII CAG'CTG Cuts at: 2329 3236 4411 5479 5631 5668 2329 Size: 907 1175 1068 152 37 6316 Fragments arranged by size: 6316 1175 1068 907 152 37 RsrII CG'GwC_CG Cuts at: 6376 6376 Size: 9655 SacI G_AGCT'C Cuts at: 2181 4620 2181 Size: 2439 7216 SacII CC_GC'GG Cuts at: 2551 6584 2551 Size: 4033 5622 SalI G'TCGA_C Cuts at: 1936 6997 1936 Size: 5061 4594 SanDI GG'GwC_CC Cuts at: 3346 4927 3346 Size: 1581 8074 SapI GCTCTTCn'nnn_ Cuts at: 3527 3527 Size: 9655 SbfI CC_TGCA'GG Cuts at: 5622 7007 5622 Size: 1385 8270 ScaI AGT'ACT Cuts at: 6061 7903 9615 6061 Size: 1842 1712 6101 Fragments arranged by size: 6101 1842 1712 SexAI A'CCwGG_T Cuts at: 6816 6816 Size: 9655 SfcI C'TryA_G Cuts at: 826 1045 2324 2713 2743 3362 4406 5474 5618 Size: 219 1279 389 30 619 1044 1068 144 Cuts at: 5618 7003 8138 8819 9010 826 Size: 1385 1135 681 191 1471 Fragments arranged by size: 1471 1385 1279 1135 1068 1044 681 619 389 219 191 144 30 SfoI GGC'GCC Cuts at: 2620 4990 6184 6448 2620 Size: 2370 1194 264 5827 Fragments arranged by size: 5827 2370 1194 264 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9655 SgrAI Cr'CCGG_yG Cuts at: 1700 2338 1700 Size: 638 9017 SmaI CCC'GGG Cuts at: 1872 2128 3992 5078 5308 6559 6982 1872 Size: 256 1864 1086 230 1251 423 4545 Fragments arranged by size: 4545 1864 1251 1086 423 256 230 SmlI C'TyrA_G Cuts at: 820 1017 1943 2972 3281 4949 5765 7759 8660 Size: 197 926 1029 309 1668 816 1994 901 Cuts at: 8660 8922 820 Size: 262 1553 Fragments arranged by size: 1994 1668 1553 1029 926 901 816 309 262 197 SnaBI TAC'GTA Cuts at: 493 6952 7446 493 Size: 6459 494 2702 Fragments arranged by size: 6459 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9655 SphI G_CATG'C Cuts at: 1132 7013 1132 Size: 5881 3774 SspI AAT'ATT Cuts at: 5 52 6078 7579 5 Size: 47 6026 1501 2081 Fragments arranged by size: 6026 2081 1501 47 StuI AGG'CCT Cuts at: 1517 3434 6287 1517 Size: 1917 2853 4885 Fragments arranged by size: 4885 2853 1917 StyI C'CwwG_G Cuts at: 513 1065 2022 2763 3004 3594 5461 5925 6549 Size: 552 957 741 241 590 1867 464 624 Cuts at: 6549 7094 513 Size: 545 3074 Fragments arranged by size: 3074 1867 957 741 624 590 552 545 464 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5429 5996 6203 7682 7699 7852 8037 Size: 1255 3849 567 207 1479 17 153 185 Cuts at: 8037 8482 325 Size: 445 1498 Fragments arranged by size: 3849 1498 1479 1255 567 445 207 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4146 5019 6059 Size: 274 80 33 51 1443 2169 873 1040 Cuts at: 6059 7901 9613 96 Size: 1842 1712 138 Fragments arranged by size: 2169 1842 1712 1443 1040 873 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2886 2918 6346 6378 9615 9647 2886 Size: 32 3428 32 3237 32 2894 Fragments arranged by size: 3428 3237 2894 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2957 3290 1316 Size: 1641 333 7681 Fragments arranged by size: 7681 1641 333 XbaI T'CTAG_A Cuts at: 6991 6991 Size: 9655 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2326 2569 3601 4592 5383 5620 5881 5905 6364 Size: 243 1032 991 791 237 261 24 459 Cuts at: 6364 6724 2326 Size: 360 5257 Fragments arranged by size: 5257 1032 991 791 459 360 261 243 237 24 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9655 XmaI C'CCGG_G Cuts at: 1870 2126 3990 5076 5306 6557 6980 1870 Size: 256 1864 1086 230 1251 423 4545 Fragments arranged by size: 4545 1864 1251 1086 423 256 230 XmnI GAAnn'nnTTC Cuts at: 4061 5045 7784 4061 Size: 984 2739 5932 Fragments arranged by size: 5932 2739 984 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3076 5123 7463 276 Size: 53 83 186 936 1542 2047 2340 2468 Fragments arranged by size: 2468 2340 2047 1542 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI SfiI SrfI SwaI