Restriction map for
pFN21ASDA0921
(Linear) (Six-base) MAPSORT of: KIAA0921.seq Check: 7972 from: 1 to: 4926
KIAA0921.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 423 3132 3184 3244 3588 3806 4926
Size: 423 2709 52 60 344 218 1120
Fragments arranged by size:
2709 1120 423 344 218 60 52
AatII G_ACGT'C
Cuts at: 0 1055 3102 4926
Size: 1055 2047 1824
Fragments arranged by size:
2047 1824 1055
Acc65I G'GTAC_C
Cuts at: 0 3626 4926
Size: 3626 1300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 565 1485 1999 3203 3369 4486 4926
Size: 147 418 920 514 1204 166 1117 440
Fragments arranged by size:
1204 1117 920 514 440 418 166 147
AfeI AGC'GCT
Cuts at: 0 1437 3317 3405 4926
Size: 1437 1880 88 1521
Fragments arranged by size:
1880 1521 1437 88
AflIII A'CryG_T
Cuts at: 0 2810 3329 4926
Size: 2810 519 1597
Fragments arranged by size:
2810 1597 519
AhdI GACnn_n'nnGTC
Cuts at: 0 3791 4926
Size: 3791 1135
AleI CACnn'nnGTG
Cuts at: 0 316 4153 4926
Size: 316 3837 773
Fragments arranged by size:
3837 773 316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1648 2398 4926
Size: 1648 750 2528
Fragments arranged by size:
2528 1648 750
AlwNI CAG_nnn'CTG
Cuts at: 0 588 4244 4639 4926
Size: 588 3656 395 287
Fragments arranged by size:
3656 588 395 287
ApaI G_GGCC'C
Cuts at: 0 4216 4535 4542 4654 4926
Size: 4216 319 7 112 272
Fragments arranged by size:
4216 319 272 112 7
ApaLI G'TGCA_C
Cuts at: 0 2917 4926
Size: 2917 2009
AvaI C'yCGr_G
Cuts at: 0 103 1405 1967 2754 3053 3283 4266 4534
Size: 103 1302 562 787 299 230 983 268
Cuts at: 4534 4926
Size: 392
Fragments arranged by size:
1302 983 787 562 392 299 268 230
103
AvrII C'CTAG_G
Cuts at: 0 981 4926
Size: 981 3945
BamHI G'GATC_C
Cuts at: 0 1124 4926
Size: 1124 3802
BanI G'GyrC_C
Cuts at: 0 595 991 2499 2965 3472 3626 4159 4423
Size: 595 396 1508 466 507 154 533 264
Cuts at: 4423 4926
Size: 503
Fragments arranged by size:
1508 595 533 507 503 466 396 264
154
BanII G_rGCy'C
Cuts at: 0 117 158 1174 1228 1484 2433 2534 2597
Size: 117 41 1016 54 256 949 101 63
Cuts at: 2597 3527 3743 4034 4216 4256 4535 4542 4654
Size: 930 216 291 182 40 279 7 112
Cuts at: 4654 4789 4926
Size: 135 137
Fragments arranged by size:
1016 949 930 291 279 256 216 182
137 135 117 112 101 63 54 41
40 7
BbeI G_GCGC'C
Cuts at: 0 599 2969 4163 4427 4926
Size: 599 2370 1194 264 499
Fragments arranged by size:
2370 1194 599 499 264
BbvCI CC'TCA_GC
Cuts at: 0 1928 4593 4926
Size: 1928 2665 333
Fragments arranged by size:
2665 1928 333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 97 131 1549 1583 4926
Size: 97 34 1418 34 3343
Fragments arranged by size:
3343 1418 97 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 292 593 701 1000 1417 1488 2626 4530
Size: 292 301 108 299 417 71 1138 1904
Cuts at: 4530 4563 4658 4926
Size: 33 95 268
Fragments arranged by size:
1904 1138 417 301 299 292 268 108
95 71 33
BlpI GC'TnA_GC
Cuts at: 0 157 496 1439 1550 4926
Size: 157 339 943 111 3376
Fragments arranged by size:
3376 943 339 157 111
Bme1580I G_kGCm'C
Cuts at: 0 2327 2504 2921 3475 3502 4121 4216 4535
Size: 2327 177 417 554 27 619 95 319
Cuts at: 4535 4542 4654 4926
Size: 7 112 272
Fragments arranged by size:
2327 619 554 417 319 272 177 112
95 27 7
BmgBI CAC'GTC
Cuts at: 0 474 1266 4547 4926
Size: 474 792 3281 379
Fragments arranged by size:
3281 792 474 379
BmrI ACTGGGnnnn_n'
Cuts at: 0 4028 4926
Size: 4028 898
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 429 4586 4926
Size: 429 4157 340
Fragments arranged by size:
4157 429 340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 253 1452 1769 1792 2275 2464 4039
Size: 106 147 1199 317 23 483 189 1575
Cuts at: 4039 4061 4281 4534 4926
Size: 22 220 253 392
Fragments arranged by size:
1575 1199 483 392 317 253 220 189
147 106 23 22
Bpu10I CC'TnA_GC
Cuts at: 0 987 1928 1934 2104 4593 4926
Size: 987 941 6 170 2489 333
Fragments arranged by size:
2489 987 941 333 170 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1243 2911 3727 4926
Size: 934 309 1668 816 1199
Fragments arranged by size:
1668 1199 934 816 309
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 1801 1904 3835 4487 4926
Size: 535 1266 103 1931 652 439
Fragments arranged by size:
1931 1266 652 535 439 103
BsaAI yAC'GTr
Cuts at: 0 1677 1869 2811 3332 3558 4926
Size: 1677 192 942 521 226 1368
Fragments arranged by size:
1677 1368 942 521 226 192
BsaHI Gr'CG_yC
Cuts at: 0 4 596 1052 2015 2966 3099 3122 3206
Size: 4 592 456 963 951 133 23 84
Cuts at: 3206 4160 4424 4882 4926
Size: 954 264 458 44
Fragments arranged by size:
963 954 951 592 458 456 264 133
84 44 23 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2101 2131 2470 2500 3254 3284 3415 3445
Size: 2101 30 339 30 754 30 131 30
Cuts at: 3445 3805 3835 4926
Size: 360 30 1091
Fragments arranged by size:
2101 1091 754 360 339 131 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 745 799 4036 4058 4264 4738 4926
Size: 745 54 3237 22 206 474 188
Fragments arranged by size:
3237 745 474 206 188 54 22
BseYI C'CCAG_C
Cuts at: 0 128 549 573 1167 1519 2192 3166 3352
Size: 128 421 24 594 352 673 974 186
Cuts at: 3352 4885 4926
Size: 1533 41
Fragments arranged by size:
1533 974 673 594 421 352 186 128
41 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 639 3112 3162 3228 3442 4926
Size: 639 2473 50 66 214 1484
Fragments arranged by size:
2473 1484 639 214 66 50
BsiEI CG_ry'CG
Cuts at: 0 25 255 330 929 4926
Size: 25 230 75 599 3997
Fragments arranged by size:
3997 599 230 75 25
BsiHKAI G_wGCw'C
Cuts at: 0 158 502 737 2597 2921 4926
Size: 158 344 235 1860 324 2005
Fragments arranged by size:
2005 1860 344 324 235 158
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1966 2455 3106 4926
Size: 1966 489 651 1820
Fragments arranged by size:
1966 1820 651 489
Bsp1286I G_dGCh'C
Cuts at: 0 117 158 502 737 1174 1228 1484 2327
Size: 117 41 344 235 437 54 256 843
Cuts at: 2327 2433 2504 2534 2597 2921 3475 3502 3527
Size: 106 71 30 63 324 554 27 25
Cuts at: 3527 3743 4034 4121 4216 4256 4535 4542 4654
Size: 216 291 87 95 40 279 7 112
Cuts at: 4654 4789 4926
Size: 135 137
Fragments arranged by size:
843 554 437 344 324 291 279 256
235 216 137 135 117 112 106 95
87 71 63 54 41 40 30 27
25 7
BspHI T'CATG_A
Cuts at: 0 2210 2471 4926
Size: 2210 261 2455
Fragments arranged by size:
2455 2210 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 423 1079 1226 1829 3041 3132 3184 3244
Size: 423 656 147 603 1212 91 52 60
Cuts at: 3244 3458 3588 3806 3983 4475 4926
Size: 214 130 218 177 492 451
Fragments arranged by size:
1212 656 603 492 451 423 218 214
177 147 130 91 60 52
BsrBI CCG'CTC
Cuts at: 0 558 2481 3624 3932 4230 4387 4647 4926
Size: 558 1923 1143 308 298 157 260 279
Fragments arranged by size:
1923 1143 558 308 298 279 260 157
BsrDI GCAATG_nn'
Cuts at: 0 816 2013 2772 3282 4845 4926
Size: 816 1197 759 510 1563 81
Fragments arranged by size:
1563 1197 816 759 510 81
BsrFI r'CCGG_y
Cuts at: 0 14 291 315 1511 2062 3612 4470 4926
Size: 14 277 24 1196 551 1550 858 456
Fragments arranged by size:
1550 1196 858 551 456 277 24 14
BsrGI T'GTAC_A
Cuts at: 0 2123 2996 4926
Size: 2123 873 1930
Fragments arranged by size:
2123 1930 873
BssHII G'CGCG_C
Cuts at: 0 70 72 124 186 188 361 617 625
Size: 70 2 52 62 2 173 256 8
Cuts at: 625 4192 4926
Size: 3567 734
Fragments arranged by size:
3567 734 256 173 70 62 52 8
2 2
BssSI C'ACGA_G
Cuts at: 0 2533 3357 4926
Size: 2533 824 1569
Fragments arranged by size:
2533 1569 824
BstAPI GCAn_nnn'nTGC
Cuts at: 0 342 635 719 3176 4926
Size: 342 293 84 2457 1750
Fragments arranged by size:
2457 1750 342 293 84
BstEII G'GTnAC_C
Cuts at: 0 1059 1104 3793 4926
Size: 1059 45 2689 1133
Fragments arranged by size:
2689 1133 1059 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1116 1693 1841 3045 3861 3885 3903 4701
Size: 1116 577 148 1204 816 24 18 798
Cuts at: 4701 4840 4926
Size: 139 86
Fragments arranged by size:
1204 1116 816 798 577 148 139 86
24 18
BstYI r'GATC_y
Cuts at: 0 1124 1615 4926
Size: 1124 491 3311
Fragments arranged by size:
3311 1124 491
Bsu36I CC'TnA_GG
Cuts at: 0 1802 4402 4926
Size: 1802 2600 524
Fragments arranged by size:
2600 1802 524
BtgI C'CryG_G
Cuts at: 0 525 740 1571 2096 2495 2723 3377 3476
Size: 525 215 831 525 399 228 654 99
Cuts at: 3476 3845 3902 4094 4310 4418 4558 4694 4926
Size: 369 57 192 216 108 140 136 232
Fragments arranged by size:
831 654 525 525 399 369 232 228
216 215 192 140 136 108 99 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3099 4019 4926
Size: 3099 920 907
Fragments arranged by size:
3099 920 907
BtsI GCAGTG_nn'
Cuts at: 0 115 1990 3226 4670 4926
Size: 115 1875 1236 1444 256
Fragments arranged by size:
1875 1444 1236 256 115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4533 4568 4926
Size: 4533 35 358
Fragments arranged by size:
4533 358 35
DraIII CAC_nnn'GTG
Cuts at: 0 342 2355 2811 2889 3561 4926
Size: 342 2013 456 78 672 1365
Fragments arranged by size:
2013 1365 672 456 342 78
DrdI GACnn_nn'nnGTC
Cuts at: 0 1057 2776 2863 4926
Size: 1057 1719 87 2063
Fragments arranged by size:
2063 1719 1057 87
EaeI y'GGCC_r
Cuts at: 0 22 252 293 320 437 536 926 1587
Size: 22 230 41 27 117 99 390 661
Cuts at: 1587 1742 2216 2544 2627 3374 3610 3758 3848
Size: 155 474 328 83 747 236 148 90
Cuts at: 3848 3872 3905 4097 4926
Size: 24 33 192 829
Fragments arranged by size:
829 747 661 474 390 328 236 230
192 155 148 117 99 90 83 41
33 27 24 22
EagI C'GGCC_G
Cuts at: 0 22 252 926 4926
Size: 22 230 674 4000
Fragments arranged by size:
4000 674 230 22
EarI CTCTTCn'nnn_
Cuts at: 0 722 1504 1894 2710 3179 4926
Size: 722 782 390 816 469 1747
Fragments arranged by size:
1747 816 782 722 469 390
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4440 4926
Size: 4440 486
EcoICRI GAG'CTC
Cuts at: 0 156 2595 4926
Size: 156 2439 2331
Fragments arranged by size:
2439 2331 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 147 253 565 1452 1485 1769 1792
Size: 106 41 106 312 887 33 284 23
Cuts at: 1792 1999 2275 2464 3203 3369 4039 4061 4281
Size: 207 276 189 739 166 670 22 220
Cuts at: 4281 4486 4534 4926
Size: 205 48 392
Fragments arranged by size:
887 739 670 392 312 284 276 220
207 205 189 166 106 106 48 41
33 23 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1312 1927 4926
Size: 1312 615 2999
Fragments arranged by size:
2999 1312 615
EcoO109I rG'GnC_Cy
Cuts at: 0 585 1002 1323 1884 1920 2302 2904 3265
Size: 585 417 321 561 36 382 602 361
Cuts at: 3265 4014 4212 4531 4538 4539 4565 4636 4650
Size: 749 198 319 7 1 26 71 14
Cuts at: 4650 4863 4926
Size: 213 63
Fragments arranged by size:
749 602 585 561 417 382 361 321
319 213 198 71 63 36 26 14
7 1
FspI TGC'GCA
Cuts at: 0 172 4926
Size: 172 4754
HaeII r_GCGC'y
Cuts at: 0 64 376 490 599 1423 1439 2969 3319
Size: 64 312 114 109 824 16 1530 350
Cuts at: 3319 3407 3431 3757 4163 4427 4732 4926
Size: 88 24 326 406 264 305 194
Fragments arranged by size:
1530 824 406 350 326 312 305 264
194 114 109 88 64 24 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1600 1632 2158 2190 3254 3286 3305 3337
Size: 1600 32 526 32 1064 32 19 32
Cuts at: 3337 3351 3383 3415 3447 3777 3805 3809 3837
Size: 14 32 32 32 330 28 4 28
Cuts at: 3837 4771 4803 4926
Size: 934 32 123
Fragments arranged by size:
1600 1064 934 526 330 123 32 32
32 32 32 32 32 32 28 28
19 14 4
HincII GTy'rAC
Cuts at: 0 939 2289 3618 4926
Size: 939 1350 1329 1308
Fragments arranged by size:
1350 1329 1308 939
KasI G'GCGC_C
Cuts at: 0 595 2965 4159 4423 4926
Size: 595 2370 1194 264 503
Fragments arranged by size:
2370 1194 595 503 264
KpnI G_GTAC'C
Cuts at: 0 3630 4926
Size: 3630 1296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2531 4926
Size: 2531 2395
MscI TGG'CCA
Cuts at: 0 439 538 1744 2218 2546 3874 3907 4099
Size: 439 99 1206 474 328 1328 33 192
Cuts at: 4099 4926
Size: 827
Fragments arranged by size:
1328 1206 827 474 439 328 192 99
33
MslI CAynn'nnrTG
Cuts at: 0 316 1114 1714 2125 2525 2960 3361 3883
Size: 316 798 600 411 400 435 401 522
Cuts at: 3883 3901 4153 4699 4705 4926
Size: 18 252 546 6 221
Fragments arranged by size:
798 600 546 522 435 411 401 400
316 252 221 18 6
MspA1I CmG'CkG
Cuts at: 0 39 149 306 332 527 612 1213 1361
Size: 39 110 157 26 195 85 601 148
Cuts at: 1361 1430 1554 2196 2388 3399 3456 3608 3645
Size: 69 124 642 192 1011 57 152 37
Cuts at: 3645 4560 4926
Size: 915 366
Fragments arranged by size:
1011 915 642 601 366 195 192 157
152 148 124 110 85 69 57 39
37 26
NaeI GCC'GGC
Cuts at: 0 293 1513 4472 4926
Size: 293 1220 2959 454
Fragments arranged by size:
2959 1220 454 293
NarI GG'CG_CC
Cuts at: 0 596 2966 4160 4424 4926
Size: 596 2370 1194 264 502
Fragments arranged by size:
2370 1194 596 502 264
NcoI C'CATG_G
Cuts at: 0 740 1571 3902 4926
Size: 740 831 2331 1024
Fragments arranged by size:
2331 1024 831 740
NdeI CA'TA_TG
Cuts at: 0 2435 4926
Size: 2435 2491
NgoMIV G'CCGG_C
Cuts at: 0 291 1511 4470 4926
Size: 291 1220 2959 456
Fragments arranged by size:
2959 1220 456 291
PasI CC'CwG_GG
Cuts at: 0 4429 4926
Size: 4429 497
PfoI T'CCnGG_A
Cuts at: 0 7 230 4676 4926
Size: 7 223 4446 250
Fragments arranged by size:
4446 250 223 7
PmlI CAC'GTG
Cuts at: 0 1677 3332 3558 4926
Size: 1677 1655 226 1368
Fragments arranged by size:
1677 1655 1368 226
PpuMI rG'GwC_Cy
Cuts at: 0 1323 1884 2904 4014 4926
Size: 1323 561 1020 1110 912
Fragments arranged by size:
1323 1110 1020 912 561
PspOMI G'GGCC_C
Cuts at: 0 4212 4531 4538 4650 4926
Size: 4212 319 7 112 276
Fragments arranged by size:
4212 319 276 112 7
PstI C_TGCA'G
Cuts at: 0 305 694 724 1343 2387 3455 3599 4926
Size: 305 389 30 619 1044 1068 144 1327
Fragments arranged by size:
1327 1068 1044 619 389 305 144 30
PvuII CAG'CTG
Cuts at: 0 306 1213 2388 3456 3608 3645 4926
Size: 306 907 1175 1068 152 37 1281
Fragments arranged by size:
1281 1175 1068 907 306 152 37
RsrII CG'GwC_CG
Cuts at: 0 4353 4926
Size: 4353 573
SacI G_AGCT'C
Cuts at: 0 158 2597 4926
Size: 158 2439 2329
Fragments arranged by size:
2439 2329 158
SacII CC_GC'GG
Cuts at: 0 528 4561 4926
Size: 528 4033 365
Fragments arranged by size:
4033 528 365
SanDI GG'GwC_CC
Cuts at: 0 1323 2904 4926
Size: 1323 1581 2022
Fragments arranged by size:
2022 1581 1323
SapI GCTCTTCn'nnn_
Cuts at: 0 1504 4926
Size: 1504 3422
SbfI CC_TGCA'GG
Cuts at: 0 3599 4926
Size: 3599 1327
ScaI AGT'ACT
Cuts at: 0 4038 4926
Size: 4038 888
SexAI A'CCwGG_T
Cuts at: 0 4793 4926
Size: 4793 133
SfcI C'TryA_G
Cuts at: 0 301 690 720 1339 2383 3451 3595 4926
Size: 301 389 30 619 1044 1068 144 1331
Fragments arranged by size:
1331 1068 1044 619 389 301 144 30
SfoI GGC'GCC
Cuts at: 0 597 2967 4161 4425 4926
Size: 597 2370 1194 264 501
Fragments arranged by size:
2370 1194 597 501 264
SgrAI Cr'CCGG_yG
Cuts at: 0 315 4926
Size: 315 4611
SmaI CCC'GGG
Cuts at: 0 105 1969 3055 3285 4536 4926
Size: 105 1864 1086 230 1251 390
Fragments arranged by size:
1864 1251 1086 390 230 105
SmlI C'TyrA_G
Cuts at: 0 949 1258 2926 3742 4926
Size: 949 309 1668 816 1184
Fragments arranged by size:
1668 1184 949 816 309
SspI AAT'ATT
Cuts at: 0 4055 4926
Size: 4055 871
StuI AGG'CCT
Cuts at: 0 1411 4264 4926
Size: 1411 2853 662
Fragments arranged by size:
2853 1411 662
StyI C'CwwG_G
Cuts at: 0 740 981 1571 3438 3902 4526 4926
Size: 740 241 590 1867 464 624 400
Fragments arranged by size:
1867 740 624 590 464 400 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3406 3973 4180 4926
Size: 3406 567 207 746
Fragments arranged by size:
3406 746 567 207
TatI w'GTAC_w
Cuts at: 0 2123 2996 4036 4926
Size: 2123 873 1040 890
Fragments arranged by size:
2123 1040 890 873
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 863 895 4323 4355 4926
Size: 863 32 3428 32 571
Fragments arranged by size:
3428 863 571 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 934 1267 4926
Size: 934 333 3659
Fragments arranged by size:
3659 934 333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 303 546 1578 2569 3360 3597 3858 3882
Size: 303 243 1032 991 791 237 261 24
Cuts at: 3882 4341 4701 4926
Size: 459 360 225
Fragments arranged by size:
1032 991 791 459 360 303 261 243
237 225 24
XmaI C'CCGG_G
Cuts at: 0 103 1967 3053 3283 4534 4926
Size: 103 1864 1086 230 1251 392
Fragments arranged by size:
1864 1251 1086 392 230 103
XmnI GAAnn'nnTTC
Cuts at: 0 2038 3022 4926
Size: 2038 984 1904
Fragments arranged by size:
2038 1904 984
ZraI GAC'GTC
Cuts at: 0 1053 3100 4926
Size: 1053 2047 1826
Fragments arranged by size:
2047 1826 1053
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV PasI PfoI PmlI PpuMI PspOMI PstI
PvuII RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgrAI SmaI SmlI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AloI ApoI AscI AseI
AsiSI BaeI BbsI BciVI BclI BfrBI BglII BmtI
BsaBI BsaWI BsiWI BsmI BspEI BstBI BstZ17I ClaI
DraI EcoRI EcoRV FalI FseI FspAI HindIII HpaI
MfeI MluI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI SalI SfiI SgfI SnaBI SpeI SphI SrfI
SwaI XbaI XhoI