(Circular) (Six-base) MAPSORT of: pFN21ASDA0875.seq Check: 1312 from: 1 to: 6592 pFN21ASDA0875 6592 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2204 2750 3587 2204 Size: 546 837 5209 Fragments arranged by size: 5209 837 546 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4402 278 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2040 3935 1937 Size: 103 1895 4594 Fragments arranged by size: 4594 1895 103 Acc65I G'GTAC_C Cuts at: 3913 3913 Size: 6592 AclI AA'CG_TT Cuts at: 1563 4719 5092 1563 Size: 3156 373 3063 Fragments arranged by size: 3156 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2398 2564 2861 4655 6039 6307 1632 Size: 766 166 297 1794 1384 268 1917 Fragments arranged by size: 1917 1794 1384 766 297 268 166 AflII C'TTAA_G Cuts at: 820 1017 2567 820 Size: 197 1550 4845 Fragments arranged by size: 4845 1550 197 AflIII A'CryG_T Cuts at: 1203 5491 6468 1203 Size: 4288 977 1327 Fragments arranged by size: 4288 1327 977 AgeI A'CCGG_T Cuts at: 5423 5423 Size: 6592 AhdI GACnn_n'nnGTC Cuts at: 2599 5321 2599 Size: 2722 3870 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2954 3153 1184 Size: 1770 199 4623 Fragments arranged by size: 4623 1770 199 AlwNI CAG_nnn'CTG Cuts at: 2196 2806 3244 4335 5907 2196 Size: 610 438 1091 1572 2881 Fragments arranged by size: 2881 1572 1091 610 438 ApaLI G'TGCA_C Cuts at: 4649 5805 4649 Size: 1156 5436 ApoI r'AATT_y Cuts at: 1493 2526 3901 4127 4279 6388 1493 Size: 1033 1375 226 152 2109 1697 Fragments arranged by size: 2109 1697 1375 1033 226 152 AseI AT'TA_AT Cuts at: 160 1032 5146 160 Size: 872 4114 1606 Fragments arranged by size: 4114 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 AvaI C'yCGr_G Cuts at: 1870 1943 3385 3917 1870 Size: 73 1442 532 4545 Fragments arranged by size: 4545 1442 532 73 BamHI G'GATC_C Cuts at: 3600 3922 4289 3600 Size: 322 367 5903 Fragments arranged by size: 5903 367 322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 3018 3913 5368 618 Size: 325 217 627 272 959 895 1455 1842 Fragments arranged by size: 1842 1455 959 895 627 325 272 217 BanII G_rGCy'C Cuts at: 1391 3522 3911 1391 Size: 2131 389 4072 Fragments arranged by size: 4072 2131 389 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5621 BbvCI CC'TCA_GC Cuts at: 2102 2102 Size: 6592 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4783 4817 1903 Size: 34 2846 34 3678 Fragments arranged by size: 3678 2846 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2483 3822 4484 5694 2483 Size: 1339 662 1210 3381 Fragments arranged by size: 3381 1339 1210 662 BclI T'GATC_A Cuts at: 1548 1548 Size: 6592 BfrBI ATG'CAT Cuts at: 2893 2893 Size: 6592 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2756 5203 136 Size: 107 122 71 631 605 1084 2447 1525 Fragments arranged by size: 2447 1525 1084 631 605 122 107 71 BglII A'GATC_T Cuts at: 2553 3820 6587 2553 Size: 1267 2767 2558 Fragments arranged by size: 2767 2558 1267 BlpI GC'TnA_GC Cuts at: 4009 4009 Size: 6592 Bme1580I G_kGCm'C Cuts at: 1790 4653 5809 1790 Size: 2863 1156 2573 Fragments arranged by size: 2863 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3745 1318 Size: 2427 4165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4323 5281 442 Size: 416 53 83 399 24 2906 958 1753 Fragments arranged by size: 2906 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6592 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3691 3691 Size: 6592 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2173 2777 2999 3347 3821 3845 5252 Size: 917 17 604 222 348 474 24 1407 Cuts at: 5252 1239 Size: 2579 Fragments arranged by size: 2579 1407 917 604 474 348 222 24 17 Bpu10I CC'TnA_GC Cuts at: 2102 2102 Size: 6592 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3708 4717 5582 5880 3708 Size: 1009 865 298 4420 Fragments arranged by size: 4420 1009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5255 882 Size: 617 3756 2219 Fragments arranged by size: 3756 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3133 3889 4383 493 Size: 711 1929 756 494 2702 Fragments arranged by size: 2702 1929 756 711 494 BsaBI GATnn'nnATC Cuts at: 3264 6586 3264 Size: 3322 3270 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4399 4781 5416 275 Size: 53 83 186 936 2866 382 635 1451 Fragments arranged by size: 2866 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3603 3807 5025 5423 5697 5844 6255 3603 Size: 204 1218 398 274 147 411 3940 Fragments arranged by size: 3940 1218 411 398 274 204 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3794 3824 1178 Size: 30 165 30 2391 30 3946 Fragments arranged by size: 3946 2391 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 3290 3293 3467 1186 Size: 904 1200 3 174 4311 Fragments arranged by size: 4311 1200 904 174 3 BseYI C'CCAG_C Cuts at: 2339 2741 3567 5795 6291 2339 Size: 402 826 2228 496 2640 Fragments arranged by size: 2640 2228 826 496 402 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3311 3437 3866 1163 Size: 573 1575 126 429 3889 Fragments arranged by size: 3889 1575 573 429 126 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2169 2182 4042 4803 5831 1540 Size: 275 186 168 13 1860 761 1028 2301 Fragments arranged by size: 2301 1860 1028 761 275 186 168 13 BsiHKAI G_wGCw'C Cuts at: 2397 3435 4653 4738 5809 2397 Size: 1038 1218 85 1071 3180 Fragments arranged by size: 3180 1218 1071 1038 85 BsmI GAATG_Cn' Cuts at: 3113 4103 4196 3113 Size: 990 93 5509 Fragments arranged by size: 5509 990 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2397 3435 3522 3911 4653 4738 5809 Size: 399 607 1038 87 389 742 85 1071 Cuts at: 5809 1391 Size: 2174 Fragments arranged by size: 2174 1071 1038 742 607 399 389 87 85 BspEI T'CCGG_A Cuts at: 3603 6255 3603 Size: 2652 3940 BspHI T'CATG_A Cuts at: 4481 6211 4481 Size: 1730 4862 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 2204 2547 2750 3587 3947 844 Size: 1283 77 343 203 837 360 3489 Fragments arranged by size: 3489 1283 837 360 343 203 77 BsrBI CCG'CTC Cuts at: 1394 1823 4479 1394 Size: 429 2656 3507 Fragments arranged by size: 3507 2656 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3279 5087 5261 65 Size: 1183 2031 1808 174 1396 Fragments arranged by size: 2031 1808 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 2199 5236 5423 1700 Size: 110 252 137 3037 187 2869 Fragments arranged by size: 3037 2869 252 187 137 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6592 BssSI C'ACGA_G Cuts at: 3593 4652 5664 3593 Size: 1059 1012 4521 Fragments arranged by size: 4521 1059 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6592 BstBI TT'CG_AA Cuts at: 2007 5396 2007 Size: 3389 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6592 BstXI CCAn_nnnn'nTGG Cuts at: 2779 2779 Size: 6592 BstYI r'GATC_y Cuts at: 1972 2553 3259 3600 3804 3820 3922 4289 4674 Size: 581 706 341 204 16 102 367 385 Cuts at: 4674 4691 6143 6229 6241 6587 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 706 581 385 367 346 341 204 102 86 17 16 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5083 Fragments arranged by size: 5083 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2063 487 Size: 683 56 837 5016 Fragments arranged by size: 5016 837 683 56 BtsI GCAGTG_nn' Cuts at: 4108 4902 4922 4108 Size: 794 20 5778 Fragments arranged by size: 5778 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6592 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6557 DraI TTT'AAA Cuts at: 3896 3896 Size: 6592 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5599 809 Size: 507 4283 1802 Fragments arranged by size: 4283 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2179 3124 Size: 54 1425 50 275 18 336 13 945 Cuts at: 3124 3338 3634 4039 4928 6557 8 Size: 214 296 405 889 1629 43 Fragments arranged by size: 1629 1425 945 889 405 336 296 275 214 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 2166 2179 4039 1537 Size: 275 354 13 1860 4090 Fragments arranged by size: 4090 1860 354 275 13 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 4522 1353 Size: 957 2212 3423 Fragments arranged by size: 3423 2212 957 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 5179 5553 5699 387 Size: 1064 138 498 3092 374 146 1280 Fragments arranged by size: 3092 1280 1064 498 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2173 2398 2564 2777 2861 2999 Size: 393 524 17 225 166 213 84 138 Cuts at: 2999 3347 3821 3845 4655 5252 6039 6307 1239 Size: 348 474 24 810 597 787 268 1524 Fragments arranged by size: 1524 810 787 597 524 474 393 348 268 225 213 166 138 84 24 17 EcoO109I rG'GnC_Cy Cuts at: 3571 3571 Size: 6592 EcoRI G'AATT_C Cuts at: 3901 4279 3901 Size: 378 6214 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 2514 3332 2401 Size: 113 818 5661 Fragments arranged by size: 5661 818 113 FspI TGC'GCA Cuts at: 5098 5098 Size: 6592 HaeII r_GCGC'y Cuts at: 1678 2069 5739 1678 Size: 391 3670 2531 Fragments arranged by size: 3670 2531 391 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 2941 2973 3796 Size: 32 135 32 1013 32 324 32 823 Cuts at: 3796 3828 5233 5265 5307 5339 1373 Size: 32 1405 32 42 32 2626 Fragments arranged by size: 2626 1405 1013 823 324 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2041 2116 2989 3936 4182 669 Size: 988 281 103 75 873 947 246 3079 Fragments arranged by size: 3079 988 947 873 281 246 103 75 HindIII A'AGCT_T Cuts at: 748 2496 748 Size: 1748 4844 HpaI GTT'AAC Cuts at: 4182 4182 Size: 6592 KpnI G_GTAC'C Cuts at: 3917 3917 Size: 6592 MfeI C'AATT_G Cuts at: 2305 2952 4191 2305 Size: 647 1239 4706 Fragments arranged by size: 4706 1239 647 MluI A'CGCG_T Cuts at: 6468 6468 Size: 6592 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2292 2676 5706 5890 1305 Size: 987 384 3030 184 2007 Fragments arranged by size: 3030 2007 987 384 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3340 10 Size: 54 1425 343 1508 3262 Fragments arranged by size: 3262 1508 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2777 3498 3581 4550 4909 5068 518 Size: 648 1611 721 83 969 359 159 2042 Fragments arranged by size: 2042 1611 969 721 648 359 159 83 MspA1I CmG'CkG Cuts at: 1606 2106 2229 3094 4008 4685 5833 6078 1606 Size: 500 123 865 914 677 1148 245 2120 Fragments arranged by size: 2120 1148 914 865 677 500 245 123 NaeI GCC'GGC Cuts at: 2064 2201 2064 Size: 137 6455 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5083 Fragments arranged by size: 5083 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6592 NgoMIV G'CCGG_C Cuts at: 2062 2199 2062 Size: 137 6455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6592 NotI GC'GGCC_GC Cuts at: 4039 4039 Size: 6592 NsiI A_TGCA'T Cuts at: 2895 2895 Size: 6592 NspI r_CATG'y Cuts at: 1132 1229 2935 3950 5495 1132 Size: 97 1706 1015 1545 2229 Fragments arranged by size: 2229 1706 1545 1015 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6592 PciI A'CATG_T Cuts at: 5491 5491 Size: 6592 PflMI CCAn_nnn'nTGG Cuts at: 1108 2806 1108 Size: 1698 4894 PmeI GTTT'AAAC Cuts at: 3896 3896 Size: 6592 PmlI CAC'GTG Cuts at: 3133 3133 Size: 6592 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4661 4693 6174 6206 4661 Size: 32 1481 32 5047 Fragments arranged by size: 5047 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3571 3571 Size: 6592 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6592 PsiI TTA'TAA Cuts at: 4162 4162 Size: 6592 PstI C_TGCA'G Cuts at: 830 3142 3944 830 Size: 2312 802 3478 Fragments arranged by size: 3478 2312 802 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6592 PvuII CAG'CTG Cuts at: 2106 3094 2106 Size: 988 5604 SalI G'TCGA_C Cuts at: 1936 2039 3934 1936 Size: 103 1895 4594 Fragments arranged by size: 4594 1895 103 SanDI GG'GwC_CC Cuts at: 3571 3571 Size: 6592 SbfI CC_TGCA'GG Cuts at: 3944 3944 Size: 6592 ScaI AGT'ACT Cuts at: 2636 3116 4840 6552 2636 Size: 480 1724 1712 2676 Fragments arranged by size: 2676 1724 1712 480 SexAI A'CCwGG_T Cuts at: 3285 3411 3285 Size: 126 6466 SfcI C'TryA_G Cuts at: 826 1045 2284 3138 3940 5075 5756 5947 826 Size: 219 1239 854 802 1135 681 191 1471 Fragments arranged by size: 1471 1239 1135 854 802 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6592 SmaI CCC'GGG Cuts at: 1872 3387 3919 1872 Size: 1515 532 4545 Fragments arranged by size: 4545 1515 532 SmlI C'TyrA_G Cuts at: 820 1017 1943 2567 3723 4696 5597 5859 820 Size: 197 926 624 1156 973 901 262 1553 Fragments arranged by size: 1553 1156 973 926 901 624 262 197 SnaBI TAC'GTA Cuts at: 493 3889 4383 493 Size: 3396 494 2702 Fragments arranged by size: 3396 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6592 SphI G_CATG'C Cuts at: 1132 3950 1132 Size: 2818 3774 SspI AAT'ATT Cuts at: 5 52 3853 4516 5 Size: 47 3801 663 2081 Fragments arranged by size: 3801 2081 663 47 StuI AGG'CCT Cuts at: 1517 2573 1517 Size: 1056 5536 StyI C'CwwG_G Cuts at: 513 1065 2022 3022 4031 513 Size: 552 957 1000 1009 3074 Fragments arranged by size: 3074 1009 1000 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4619 4636 4789 4974 5419 325 Size: 1255 3039 17 153 185 445 1498 Fragments arranged by size: 3039 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2139 2634 3114 Size: 274 80 33 51 1443 162 495 480 Cuts at: 3114 4838 6550 96 Size: 1724 1712 138 Fragments arranged by size: 1724 1712 1443 495 480 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6552 6584 6552 Size: 32 6560 Tth111I GACn'n_nGTC Cuts at: 1316 2300 1316 Size: 984 5608 XbaI T'CTAG_A Cuts at: 3427 3928 3427 Size: 501 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3583 3583 Size: 6592 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6592 XmaI C'CCGG_G Cuts at: 1870 3385 3917 1870 Size: 1515 532 4545 Fragments arranged by size: 4545 1515 532 XmnI GAAnn'nnTTC Cuts at: 4721 4721 Size: 6592 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4400 276 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI