Restriction map for
pFN21ASDA0875
(Linear) (Six-base) MAPSORT of: KIAA0875.seq Check: 5130 from: 1 to: 1863
KIAA0875.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 181 727 1564 1863
Size: 181 546 837 299
Fragments arranged by size:
837 546 299 181
AccI GT'mk_AC
Cuts at: 0 17 1863
Size: 17 1846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 541 838 1863
Size: 375 166 297 1025
Fragments arranged by size:
1025 375 297 166
AflII C'TTAA_G
Cuts at: 0 544 1863
Size: 544 1319
AhdI GACnn_n'nnGTC
Cuts at: 0 576 1863
Size: 576 1287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 931 1130 1863
Size: 931 199 733
Fragments arranged by size:
931 733 199
AlwNI CAG_nnn'CTG
Cuts at: 0 173 783 1221 1863
Size: 173 610 438 642
Fragments arranged by size:
642 610 438 173
ApoI r'AATT_y
Cuts at: 0 503 1863
Size: 503 1360
AvaI C'yCGr_G
Cuts at: 0 1362 1863
Size: 1362 501
BamHI G'GATC_C
Cuts at: 0 1577 1863
Size: 1577 286
BanI G'GyrC_C
Cuts at: 0 36 995 1863
Size: 36 959 868
Fragments arranged by size:
959 868 36
BanII G_rGCy'C
Cuts at: 0 1499 1863
Size: 1499 364
BbvCI CC'TCA_GC
Cuts at: 0 79 1863
Size: 79 1784
BciVI GTATCCnnnnn_n'
Cuts at: 0 460 1799 1863
Size: 460 1339 64
Fragments arranged by size:
1339 460 64
BfrBI ATG'CAT
Cuts at: 0 870 1863
Size: 870 993
BglI GCCn_nnn'nGGC
Cuts at: 0 733 1863
Size: 733 1130
BglII A'GATC_T
Cuts at: 0 530 1797 1863
Size: 530 1267 66
Fragments arranged by size:
1267 530 66
BmgBI CAC'GTC
Cuts at: 0 1722 1863
Size: 1722 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1668 1863
Size: 1668 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 150 754 976 1324 1798 1822 1863
Size: 133 17 604 222 348 474 24 41
Fragments arranged by size:
604 474 348 222 133 41 24 17
Bpu10I CC'TnA_GC
Cuts at: 0 79 1863
Size: 79 1784
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1685 1863
Size: 1685 178
BsaAI yAC'GTr
Cuts at: 0 1110 1863
Size: 1110 753
BsaBI GATnn'nnATC
Cuts at: 0 1241 1863
Size: 1241 622
BsaWI w'CCGG_w
Cuts at: 0 1580 1784 1863
Size: 1580 204 79
Fragments arranged by size:
1580 204 79
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1771 1801 1863
Size: 1771 30 62
Fragments arranged by size:
1771 62 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1267 1270 1444 1863
Size: 67 1200 3 174 419
Fragments arranged by size:
1200 419 174 67 3
BseYI C'CCAG_C
Cuts at: 0 316 718 1544 1863
Size: 316 402 826 319
Fragments arranged by size:
826 402 319 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 1414 1843 1863
Size: 1288 126 429 20
Fragments arranged by size:
1288 429 126 20
BsiEI CG_ry'CG
Cuts at: 0 146 159 1863
Size: 146 13 1704
Fragments arranged by size:
1704 146 13
BsiHKAI G_wGCw'C
Cuts at: 0 374 1412 1863
Size: 374 1038 451
Fragments arranged by size:
1038 451 374
BsmI GAATG_Cn'
Cuts at: 0 1090 1863
Size: 1090 773
Bsp1286I G_dGCh'C
Cuts at: 0 374 1412 1499 1863
Size: 374 1038 87 364
Fragments arranged by size:
1038 374 364 87
BspEI T'CCGG_A
Cuts at: 0 1580 1863
Size: 1580 283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 181 524 727 1564 1863
Size: 104 77 343 203 837 299
Fragments arranged by size:
837 343 299 203 104 77
BsrDI GCAATG_nn'
Cuts at: 0 1256 1863
Size: 1256 607
BsrFI r'CCGG_y
Cuts at: 0 39 176 1863
Size: 39 137 1687
Fragments arranged by size:
1687 137 39
BssSI C'ACGA_G
Cuts at: 0 1570 1863
Size: 1570 293
BstXI CCAn_nnnn'nTGG
Cuts at: 0 756 1863
Size: 756 1107
BstYI r'GATC_y
Cuts at: 0 530 1236 1577 1781 1797 1863
Size: 530 706 341 204 16 66
Fragments arranged by size:
706 530 341 204 66 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 40 1863
Size: 40 1823
EaeI y'GGCC_r
Cuts at: 0 143 156 1101 1315 1611 1863
Size: 143 13 945 214 296 252
Fragments arranged by size:
945 296 252 214 143 13
EagI C'GGCC_G
Cuts at: 0 143 156 1863
Size: 143 13 1707
Fragments arranged by size:
1707 143 13
EarI CTCTTCn'nnn_
Cuts at: 0 287 1863
Size: 287 1576
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 1863
Size: 64 1799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 150 375 541 754 838 976 1324
Size: 133 17 225 166 213 84 138 348
Cuts at: 1324 1798 1822 1863
Size: 474 24 41
Fragments arranged by size:
474 348 225 213 166 138 133 84
41 24 17
EcoO109I rG'GnC_Cy
Cuts at: 0 1548 1863
Size: 1548 315
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 378 491 1309 1863
Size: 378 113 818 554
Fragments arranged by size:
818 554 378 113
HaeII r_GCGC'y
Cuts at: 0 46 1863
Size: 46 1817
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 562 594 918 950 1773 1805 1863
Size: 562 32 324 32 823 32 58
Fragments arranged by size:
823 562 324 58 32 32 32
HincII GTy'rAC
Cuts at: 0 18 93 966 1863
Size: 18 75 873 897
Fragments arranged by size:
897 873 75 18
HindIII A'AGCT_T
Cuts at: 0 473 1863
Size: 473 1390
MfeI C'AATT_G
Cuts at: 0 282 929 1863
Size: 282 647 934
Fragments arranged by size:
934 647 282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 653 1863
Size: 269 384 1210
Fragments arranged by size:
1210 384 269
MscI TGG'CCA
Cuts at: 0 1317 1863
Size: 1317 546
MslI CAynn'nnrTG
Cuts at: 0 754 1475 1558 1863
Size: 754 721 83 305
Fragments arranged by size:
754 721 305 83
MspA1I CmG'CkG
Cuts at: 0 83 206 1071 1863
Size: 83 123 865 792
Fragments arranged by size:
865 792 123 83
NaeI GCC'GGC
Cuts at: 0 41 178 1863
Size: 41 137 1685
Fragments arranged by size:
1685 137 41
NgoMIV G'CCGG_C
Cuts at: 0 39 176 1863
Size: 39 137 1687
Fragments arranged by size:
1687 137 39
NsiI A_TGCA'T
Cuts at: 0 872 1863
Size: 872 991
NspI r_CATG'y
Cuts at: 0 912 1863
Size: 912 951
PflMI CCAn_nnn'nTGG
Cuts at: 0 783 1863
Size: 783 1080
PmlI CAC'GTG
Cuts at: 0 1110 1863
Size: 1110 753
PpuMI rG'GwC_Cy
Cuts at: 0 1548 1863
Size: 1548 315
PstI C_TGCA'G
Cuts at: 0 1119 1863
Size: 1119 744
PvuII CAG'CTG
Cuts at: 0 83 1071 1863
Size: 83 988 792
Fragments arranged by size:
988 792 83
SalI G'TCGA_C
Cuts at: 0 16 1863
Size: 16 1847
SanDI GG'GwC_CC
Cuts at: 0 1548 1863
Size: 1548 315
ScaI AGT'ACT
Cuts at: 0 613 1093 1863
Size: 613 480 770
Fragments arranged by size:
770 613 480
SexAI A'CCwGG_T
Cuts at: 0 1262 1388 1863
Size: 1262 126 475
Fragments arranged by size:
1262 475 126
SfcI C'TryA_G
Cuts at: 0 261 1115 1863
Size: 261 854 748
Fragments arranged by size:
854 748 261
SmaI CCC'GGG
Cuts at: 0 1364 1863
Size: 1364 499
SmlI C'TyrA_G
Cuts at: 0 544 1700 1863
Size: 544 1156 163
Fragments arranged by size:
1156 544 163
SspI AAT'ATT
Cuts at: 0 1830 1863
Size: 1830 33
StuI AGG'CCT
Cuts at: 0 550 1863
Size: 550 1313
StyI C'CwwG_G
Cuts at: 0 999 1863
Size: 999 864
TatI w'GTAC_w
Cuts at: 0 116 611 1091 1863
Size: 116 495 480 772
Fragments arranged by size:
772 495 480 116
Tth111I GACn'n_nGTC
Cuts at: 0 277 1863
Size: 277 1586
XbaI T'CTAG_A
Cuts at: 0 1404 1863
Size: 1404 459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1560 1863
Size: 1560 303
XmaI C'CCGG_G
Cuts at: 0 1362 1863
Size: 1362 501
Enzymes that do cut:
AarI AccI AcuI AflII AhdI AlfI AlwNI ApoI
AvaI BamHI BanI BanII BbvCI BciVI BfrBI BglI
BglII BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstXI BstYI
BtgZI EaeI EagI EarI EciI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NaeI NgoMIV NsiI NspI PflMI PmlI PpuMI
PstI PvuII SalI SanDI ScaI SexAI SfcI SmaI
SmlI SspI StuI StyI TatI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BcgI BclI BlpI Bme1580I BmrI BmtI BsaI
BsaHI BsiWI BsmBI BspHI BsrBI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HpaI KasI KpnI MluI NarI NcoI
NdeI NheI NotI NruI PacI PasI PciI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SapI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI TaqII TstI
XhoI XmnI ZraI