(Circular) (Six-base) MAPSORT of: pFN21ASDA0860.seq Check: 406 from: 1 to: 6352 pFN21ASDA0860 6352 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3054 3054 Size: 6352 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4162 278 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3695 1937 Size: 1758 4594 Acc65I G'GTAC_C Cuts at: 3673 3673 Size: 6352 AclI AA'CG_TT Cuts at: 1563 4479 4852 1563 Size: 2916 373 3063 Fragments arranged by size: 3063 2916 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2136 4415 5799 6067 1632 Size: 504 2279 1384 268 1917 Fragments arranged by size: 2279 1917 1384 504 268 AflII C'TTAA_G Cuts at: 820 1017 2539 820 Size: 197 1522 4633 Fragments arranged by size: 4633 1522 197 AflIII A'CryG_T Cuts at: 1203 3133 5251 6228 1203 Size: 1930 2118 977 1327 Fragments arranged by size: 2118 1930 1327 977 AgeI A'CCGG_T Cuts at: 5183 5183 Size: 6352 AhdI GACnn_n'nnGTC Cuts at: 5081 5081 Size: 6352 AleI CACnn'nnGTG Cuts at: 1166 2558 1166 Size: 1392 4960 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6352 AlwNI CAG_nnn'CTG Cuts at: 2776 2977 3301 4095 5667 2776 Size: 201 324 794 1572 3461 Fragments arranged by size: 3461 1572 794 324 201 ApaI G_GGCC'C Cuts at: 2333 3464 2333 Size: 1131 5221 ApaLI G'TGCA_C Cuts at: 3592 4409 5565 3592 Size: 817 1156 4379 Fragments arranged by size: 4379 1156 817 ApoI r'AATT_y Cuts at: 1493 2061 3661 3887 4039 6148 1493 Size: 568 1600 226 152 2109 1697 Fragments arranged by size: 2109 1697 1600 568 226 152 AseI AT'TA_AT Cuts at: 160 1032 4906 160 Size: 872 3874 1606 Fragments arranged by size: 3874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 3677 1870 Size: 73 1734 4545 Fragments arranged by size: 4545 1734 73 BamHI G'GATC_C Cuts at: 2815 3682 4049 2815 Size: 867 367 5118 Fragments arranged by size: 5118 867 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3428 3673 5128 618 Size: 325 217 627 1641 245 1455 1842 Fragments arranged by size: 1842 1641 1455 627 325 245 217 BanII G_rGCy'C Cuts at: 1391 2333 2499 2515 3249 3464 3671 1391 Size: 942 166 16 734 215 207 4072 Fragments arranged by size: 4072 942 734 215 207 166 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5381 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2854 2888 4543 4577 1903 Size: 34 917 34 1655 34 3678 Fragments arranged by size: 3678 1655 917 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2585 4244 5454 2585 Size: 1659 1210 3483 Fragments arranged by size: 3483 1659 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6352 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2424 4963 136 Size: 107 122 71 631 605 752 2539 1525 Fragments arranged by size: 2539 1525 752 631 605 122 107 71 BglII A'GATC_T Cuts at: 2119 6347 2119 Size: 4228 2124 BlpI GC'TnA_GC Cuts at: 2337 3769 2337 Size: 1432 4920 Bme1580I G_kGCm'C Cuts at: 1790 2333 2931 3464 3596 4413 5569 1790 Size: 543 598 533 132 817 1156 2573 Fragments arranged by size: 2573 1156 817 598 543 533 132 BmgBI CAC'GTC Cuts at: 1318 3591 3628 1318 Size: 2273 37 4042 Fragments arranged by size: 4042 2273 37 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2308 2925 4083 Size: 416 53 83 399 24 891 617 1158 Cuts at: 4083 5041 442 Size: 958 1753 Fragments arranged by size: 1753 1158 958 891 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3621 1056 Size: 2565 3787 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2682 2746 2849 2909 3005 3368 3439 5012 Size: 1443 64 103 60 96 363 71 1573 Cuts at: 5012 1239 Size: 2579 Fragments arranged by size: 2579 1573 1443 363 103 96 71 64 60 Bpu10I CC'TnA_GC Cuts at: 2522 2522 Size: 6352 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2978 4477 5342 5640 2978 Size: 1499 865 298 3690 Fragments arranged by size: 3690 1499 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5015 882 Size: 617 3516 2219 Fragments arranged by size: 3516 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2530 3649 4143 493 Size: 711 1326 1119 494 2702 Fragments arranged by size: 2702 1326 1119 711 494 BsaBI GATnn'nnATC Cuts at: 2549 6346 2549 Size: 3797 2555 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4159 4541 5176 275 Size: 53 83 186 936 2626 382 635 1451 Fragments arranged by size: 2626 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4785 5183 5457 5604 6015 4785 Size: 398 274 147 411 5122 Fragments arranged by size: 5122 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3503 3533 1178 Size: 30 165 30 2100 30 3997 Fragments arranged by size: 3997 2100 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2819 1186 Size: 1633 4719 BseYI C'CCAG_C Cuts at: 2237 2332 3248 5555 6051 2237 Size: 95 916 2307 496 2538 Fragments arranged by size: 2538 2307 916 496 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5779 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3611 3802 4563 5591 1540 Size: 275 186 1610 191 761 1028 2301 Fragments arranged by size: 2301 1610 1028 761 275 191 186 BsiHKAI G_wGCw'C Cuts at: 2499 2885 3061 3216 3596 4413 4498 5569 2499 Size: 386 176 155 380 817 85 1071 3282 Fragments arranged by size: 3282 1071 817 386 380 176 155 85 BsmI GAATG_Cn' Cuts at: 3863 3956 3863 Size: 93 6259 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2333 2499 2515 2885 2931 3061 3216 Size: 399 543 166 16 370 46 130 155 Cuts at: 3216 3249 3464 3596 3671 4413 4498 5569 1391 Size: 33 215 132 75 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 543 399 370 215 166 155 132 130 85 75 46 33 16 BspEI T'CCGG_A Cuts at: 6015 6015 Size: 6352 BspHI T'CATG_A Cuts at: 4241 5971 4241 Size: 1730 4622 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2630 2679 3054 3707 844 Size: 1786 49 375 653 3489 Fragments arranged by size: 3489 1786 653 375 49 BsrBI CCG'CTC Cuts at: 1394 1823 4239 1394 Size: 429 2416 3507 Fragments arranged by size: 3507 2416 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4847 5021 65 Size: 1183 3599 174 1396 Fragments arranged by size: 3599 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2611 3431 3514 3610 4996 5183 1700 Size: 110 801 820 83 96 1386 187 2869 Fragments arranged by size: 2869 1386 820 801 187 110 96 83 BsrGI T'GTAC_A Cuts at: 96 2271 96 Size: 2175 4177 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 3290 4412 5424 3290 Size: 1122 1012 4218 Fragments arranged by size: 4218 1122 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3600 794 Size: 2806 3546 BstBI TT'CG_AA Cuts at: 2007 5156 2007 Size: 3149 3203 BstEII G'GTnAC_C Cuts at: 1184 2656 1184 Size: 1472 4880 BstXI CCAn_nnnn'nTGG Cuts at: 2827 3050 3392 2827 Size: 223 342 5787 Fragments arranged by size: 5787 342 223 BstYI r'GATC_y Cuts at: 1972 2119 2544 2815 3682 4049 4434 4451 5903 Size: 147 425 271 867 367 385 17 1452 Cuts at: 5903 5989 6001 6347 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 867 425 385 367 346 271 147 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2676 2802 2860 2676 Size: 126 58 6168 Fragments arranged by size: 6168 126 58 BtgI C'CryG_G Cuts at: 513 1065 2022 2709 513 Size: 552 957 687 4156 Fragments arranged by size: 4156 957 687 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2468 487 Size: 683 56 1242 4371 Fragments arranged by size: 4371 1242 683 56 BtsI GCAGTG_nn' Cuts at: 2065 2911 3081 3216 3868 4662 4682 2065 Size: 846 170 135 652 794 20 3735 Fragments arranged by size: 3735 846 794 652 170 135 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6352 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6317 DraI TTT'AAA Cuts at: 3656 3656 Size: 6352 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5359 809 Size: 507 4043 1802 Fragments arranged by size: 4043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2613 2778 3535 Size: 54 1425 50 275 18 783 165 757 Cuts at: 3535 3608 3799 4688 6317 8 Size: 73 191 889 1629 43 Fragments arranged by size: 1629 1425 889 783 757 275 191 165 73 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3608 3799 1537 Size: 275 1796 191 4090 Fragments arranged by size: 4090 1796 275 191 EarI CTCTTCn'nnn_ Cuts at: 1353 4282 1353 Size: 2929 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4939 5313 5459 387 Size: 1064 138 3350 374 146 1280 Fragments arranged by size: 3350 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2497 2497 Size: 6352 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2682 2746 2849 2909 3005 3368 Size: 393 504 546 64 103 60 96 363 Cuts at: 3368 3439 4415 5012 5799 6067 1239 Size: 71 976 597 787 268 1524 Fragments arranged by size: 1524 976 787 597 546 504 393 363 268 103 96 71 64 60 EcoNI CCTnn'n_nnAGG Cuts at: 2675 2675 Size: 6352 EcoO109I rG'GnC_Cy Cuts at: 2439 3461 2439 Size: 1022 5330 EcoRI G'AATT_C Cuts at: 2061 3661 4039 2061 Size: 1600 378 4374 Fragments arranged by size: 4374 1600 378 EcoRV GAT'ATC Cuts at: 2079 2079 Size: 6352 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2531 2531 Size: 6352 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 5499 1678 Size: 3821 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4993 5025 5067 5099 1373 Size: 32 135 32 3421 32 42 32 2626 Fragments arranged by size: 3421 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3696 3942 669 Size: 988 281 1758 246 3079 Fragments arranged by size: 3079 1758 988 281 246 HindIII A'AGCT_T Cuts at: 748 3310 748 Size: 2562 3790 HpaI GTT'AAC Cuts at: 3942 3942 Size: 6352 KpnI G_GTAC'C Cuts at: 3677 3677 Size: 6352 MfeI C'AATT_G Cuts at: 3951 3951 Size: 6352 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2574 5466 5650 1305 Size: 1269 2892 184 2007 Fragments arranged by size: 2892 2007 1269 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4530 Fragments arranged by size: 4530 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2558 4310 4669 4828 518 Size: 648 1392 1752 359 159 2042 Fragments arranged by size: 2042 1752 1392 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2237 2336 3526 3607 3768 4445 5593 Size: 478 153 99 1190 81 161 677 1148 Cuts at: 5593 5838 1606 Size: 245 2120 Fragments arranged by size: 2120 1190 1148 677 478 245 161 153 99 81 NaeI GCC'GGC Cuts at: 2613 2613 Size: 6352 NcoI C'CATG_G Cuts at: 513 1065 2022 2709 513 Size: 552 957 687 4156 Fragments arranged by size: 4156 957 687 552 NdeI CA'TA_TG Cuts at: 387 3261 387 Size: 2874 3478 NgoMIV G'CCGG_C Cuts at: 2611 2611 Size: 6352 NheI G'CTAG_C Cuts at: 1052 3617 1052 Size: 2565 3787 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 3137 3710 5255 1132 Size: 97 1908 573 1545 2229 Fragments arranged by size: 2229 1908 1545 573 97 PasI CC'CwG_GG Cuts at: 1348 2326 2729 1348 Size: 978 403 4971 Fragments arranged by size: 4971 978 403 PciI A'CATG_T Cuts at: 3133 5251 3133 Size: 2118 4234 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6352 PfoI T'CCnGG_A Cuts at: 2811 3416 2811 Size: 605 5747 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PmlI CAC'GTG Cuts at: 2530 2530 Size: 6352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4421 4453 5934 5966 4421 Size: 32 1481 32 4807 Fragments arranged by size: 4807 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6352 PsiI TTA'TAA Cuts at: 3922 3922 Size: 6352 PspOMI G'GGCC_C Cuts at: 2329 3460 2329 Size: 1131 5221 PstI C_TGCA'G Cuts at: 830 2694 2772 3704 830 Size: 1864 78 932 3478 Fragments arranged by size: 3478 1864 932 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 PvuII CAG'CTG Cuts at: 2084 2237 2336 3526 2084 Size: 153 99 1190 4910 Fragments arranged by size: 4910 1190 153 99 SacI G_AGCT'C Cuts at: 2499 2499 Size: 6352 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SbfI CC_TGCA'GG Cuts at: 3704 3704 Size: 6352 ScaI AGT'ACT Cuts at: 4600 6312 4600 Size: 1712 4640 SfcI C'TryA_G Cuts at: 826 1045 2212 2690 2768 3700 4835 5516 5707 Size: 219 1167 478 78 932 1135 681 191 Cuts at: 5707 826 Size: 1471 Fragments arranged by size: 1471 1167 1135 932 681 478 219 191 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 3679 1872 Size: 1807 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2539 2993 4456 5357 5619 820 Size: 197 926 596 454 1463 901 262 1553 Fragments arranged by size: 1553 1463 926 901 596 454 262 197 SnaBI TAC'GTA Cuts at: 493 3649 4143 493 Size: 3156 494 2702 Fragments arranged by size: 3156 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 3710 1132 Size: 2578 3774 SspI AAT'ATT Cuts at: 5 52 4276 5 Size: 47 4224 2081 Fragments arranged by size: 4224 2081 47 StuI AGG'CCT Cuts at: 1517 3424 1517 Size: 1907 4445 StyI C'CwwG_G Cuts at: 513 1065 2022 2370 2709 3324 3337 3391 3791 Size: 552 957 348 339 615 13 54 400 Cuts at: 3791 513 Size: 3074 Fragments arranged by size: 3074 957 615 552 400 348 339 54 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2570 3551 4379 4396 4549 4734 5179 Size: 1255 990 981 828 17 153 185 445 Cuts at: 5179 325 Size: 1498 Fragments arranged by size: 1498 1255 990 981 828 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 4598 6310 Size: 274 80 33 51 1443 294 2327 1712 Cuts at: 6310 96 Size: 138 Fragments arranged by size: 2327 1712 1443 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6312 6344 6312 Size: 32 6320 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6352 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3187 3187 Size: 6352 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6352 XmaI C'CCGG_G Cuts at: 1870 3677 1870 Size: 1807 4545 XmnI GAAnn'nnTTC Cuts at: 4481 4481 Size: 6352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4160 276 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI