Restriction map for
pFN21ASDA0846
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII AccII AccIII AclI AflII
AgeI AocI Aor51HI ApaI AscI
AseI AsnI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BfrI BglI BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraIII EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KspI MamI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SspBI
SspI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1194 GGTACC G^GTACC
AccI 1996 GTAGAC GT^[AC][GT]AC
AflIII 339 ACACGT A^C[GA][CT]GT
AhdI 1876 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 48 GTGCAC G^TGCAC
AlwNI 1859 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 48 GTGCAC G^TGCAC
Asp700I 157 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1194 GGTACC G^GTACC
AspEI 1876 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 454 GAGCCC G[GA]GC[CT]^C
BbeI 1644 GGCGCC GGCGC^C
BcgI 1521 CGAACACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 469 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 469 GCTGAGC GC^T[ACGT]AGC
BspDI 579 ATCGAT AT^CGAT
BspHI 230 TCATGA T^CATGA
BspMI 1073 ACCTGC ACCTGC(4/8)
BsrDI 200 GCAATG GCAATG(2/0)
BstAPI 845 GCAATTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 469 GCTGAGC GC^T[ACGT]AGC
ClaI 579 ATCGAT AT^CGAT
DraII 1882 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 1234 GACCCCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1876 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1882 GGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 1531 GCGGG CCCGC(4/6)
HaeII 1644 GGCGCC [GA]GCGC^[CT]
HgaI 1176 GACGC GACGC(5/10)
HincII 320 GTCAAC GT[CT]^[GA]AC
HindII 320 GTCAAC GT[CT]^[GA]AC
KasI 1644 GGCGCC G^GCGCC
KpnI 1194 GGTACC GGTAC^C
MaeII 341 ACGT A^CGT
MfeI 1587 CAATTG C^AATTG
MunI 1587 CAATTG C^AATTG
NarI 1644 GGCGCC GG^CGCC
NcoI 543 CCATGG C^CATGG
PflMI 615 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1882 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 230 TCATGA T^CATGA
SapI 1784 GCTCTTC GCTCTTC(1/4)
SbfI 1625 CCTGCAGG CCTGCA^GG
SfoI 1644 GGCGCC GGC^GCC
SmaI 1640 CCCGGG CCC^GGG
SpeI 1017 ACTAGT A^CTAGT
SphI 1977 GCATGC GCATG^C
Sse8387I 1625 CCTGCAGG CCTGCA^GG
Van91I 615 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1181 CCAACAAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1640 CCCGGG C^CCGGG
XmaI 1640 CCCGGG C^CCGGG
XmnI 157 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 861 CCGC CCGC(-3/-1)
1531 GCGG
AcyI 1176 GACGCC G[GA]^CG[CT]C
1644 GGCGCC
AspHI 48 GTGCAC G[AT]GC[AT]^C
472 GAGCAC
AvaI 1640 CCCGGG C^[CT]CG[GA]G
1878 CTCGGG
BanI 1194 GGTACC G^G[CT][GA]CC
1644 GGCGCC
BciVI 1561 GGATAC GTATCC(6/5)
412 GTATCC
BclI 1067 TGATCA T^GATCA
1293 TGATCA
BglII 218 AGATCT A^GATCT
1796 AGATCT
BsaHI 1176 GACGCC G[GA]^CG[CT]C
1644 GGCGCC
BseRI 834 CTCCTC GAGGAG(10/8)
961 GAGGAG
BsiHKAI 48 GTGCAC G[AT]GC[AT]^C
472 GAGCAC
BsmI 1032 GAATGC GAATGC(1/-1)
365 GCATTC
BsoBI 1640 CCCGGG C^[CT]CG[GA]G
1878 CTCGGG
CfoI 1645 GCGC GCG^C
1702 GCGC
DsaI 543 CCATGG C^C[GA][CT]GG
1238 CCACGG
FbaI 1067 TGATCA T^GATCA
1293 TGATCA
HapII 1641 CCGG C^CGG
2001 CCGG
HhaI 1645 GCGC GCG^C
1702 GCGC
Hin1I 1176 GACGCC G[GA]^CG[CT]C
1644 GGCGCC
HinP1I 1645 GCGC G^CGC
1702 GCGC
HpaII 1641 CCGG C^CGG
2001 CCGG
MspI 1641 CCGG C^CGG
2001 CCGG
NspI 170 GCATGT [GA]CATG^[CT]
1977 GCATGC
SfaNI 120 GATGC GCATC(5/9)
1221 GATGC
SmlI 1504 CTCAAG C^T[CT][GA]AG
1653 CTTGAG
StuI 738 AGGCCT AGG^CCT
866 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII