(Circular) (Six-base) MAPSORT of: pFN21ASDA0846.seq Check: 881 from: 1 to: 6796 pFN21ASDA0846 6796 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4606 278 Size: 53 83 186 936 3070 2468 Fragments arranged by size: 3070 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4020 4139 1937 Size: 2083 119 4594 Fragments arranged by size: 4594 2083 119 Acc65I G'GTAC_C Cuts at: 3217 4117 3217 Size: 900 5896 AclI AA'CG_TT Cuts at: 1563 4923 5296 1563 Size: 3360 373 3063 Fragments arranged by size: 3360 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2291 2705 2799 3150 4859 6243 6511 1632 Size: 659 414 94 351 1709 1384 268 1917 Fragments arranged by size: 1917 1709 1384 659 414 351 268 94 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6599 AflIII A'CryG_T Cuts at: 1203 2362 5695 6672 1203 Size: 1159 3333 977 1327 Fragments arranged by size: 3333 1327 1159 977 AgeI A'CCGG_T Cuts at: 5627 5627 Size: 6796 AhdI GACnn_n'nnGTC Cuts at: 3904 5525 3904 Size: 1621 5175 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6796 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6796 AlwNI CAG_nnn'CTG Cuts at: 3887 4539 6111 3887 Size: 652 1572 4572 Fragments arranged by size: 4572 1572 652 ApaLI G'TGCA_C Cuts at: 2071 4853 6009 2071 Size: 2782 1156 2858 Fragments arranged by size: 2858 2782 1156 ApoI r'AATT_y Cuts at: 1493 2228 2324 2731 3172 4105 4331 4483 6592 Size: 735 96 407 441 933 226 152 2109 Cuts at: 6592 1493 Size: 1697 Fragments arranged by size: 2109 1697 933 735 441 407 226 152 96 AseI AT'TA_AT Cuts at: 160 1032 5350 160 Size: 872 4318 1606 Fragments arranged by size: 4318 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6796 AvaI C'yCGr_G Cuts at: 1870 1943 3663 3901 4121 1870 Size: 73 1720 238 220 4545 Fragments arranged by size: 4545 1720 238 220 73 BamHI G'GATC_C Cuts at: 4126 4493 4126 Size: 367 6429 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3217 3667 4117 5572 618 Size: 325 217 627 1430 450 450 1455 1842 Fragments arranged by size: 1842 1455 1430 627 450 450 325 217 BanII G_rGCy'C Cuts at: 1391 2481 4115 1391 Size: 1090 1634 4072 Fragments arranged by size: 4072 1634 1090 BbeI G_GCGC'C Cuts at: 3671 3671 Size: 6796 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5825 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3533 3567 4987 5021 1903 Size: 34 1596 34 1420 34 3678 Fragments arranged by size: 3678 1596 1420 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2446 3578 4688 5898 2446 Size: 1132 1110 1210 3344 Fragments arranged by size: 3344 1210 1132 1110 BclI T'GATC_A Cuts at: 1548 3090 3316 1548 Size: 1542 226 5028 Fragments arranged by size: 5028 1542 226 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5407 136 Size: 107 122 71 631 605 3735 1525 Fragments arranged by size: 3735 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2241 3819 6791 2241 Size: 1578 2972 2246 Fragments arranged by size: 2972 2246 1578 BlpI GC'TnA_GC Cuts at: 2493 4213 2493 Size: 1720 5076 Bme1580I G_kGCm'C Cuts at: 1790 2075 4031 4857 6013 1790 Size: 285 1956 826 1156 2573 Fragments arranged by size: 2573 1956 1156 826 285 BmgBI CAC'GTC Cuts at: 1318 2365 1318 Size: 1047 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4527 5485 442 Size: 416 53 83 399 24 3110 958 1753 Fragments arranged by size: 3110 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6796 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2872 2872 Size: 6796 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 2495 2531 2597 3182 3245 3771 5456 Size: 993 263 36 66 585 63 526 1685 Cuts at: 5456 1239 Size: 2579 Fragments arranged by size: 2579 1685 993 585 526 263 66 63 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3512 3697 4921 5786 6084 3512 Size: 185 1224 865 298 4224 Fragments arranged by size: 4224 1224 865 298 185 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2789 2860 4058 5459 882 Size: 617 1290 71 1198 1401 2219 Fragments arranged by size: 2219 1401 1290 1198 617 71 BsaAI yAC'GTr Cuts at: 493 1204 4093 4587 493 Size: 711 2889 494 2702 Fragments arranged by size: 2889 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6790 6790 Size: 6796 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3200 3668 4603 4985 Size: 53 83 186 936 1667 468 935 382 Cuts at: 4985 5620 275 Size: 635 1451 Fragments arranged by size: 1667 1451 936 935 635 468 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5229 5627 5901 6048 6459 5229 Size: 398 274 147 411 5566 Fragments arranged by size: 5566 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3005 3035 1178 Size: 30 165 30 1602 30 4939 Fragments arranged by size: 4939 1602 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2848 2999 1186 Size: 1662 151 4983 Fragments arranged by size: 4983 1662 151 BseYI C'CCAG_C Cuts at: 2667 3721 3891 5999 6495 2667 Size: 1054 170 2108 496 2968 Fragments arranged by size: 2968 2108 1054 496 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6223 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4246 5007 6035 1540 Size: 275 186 2245 761 1028 2301 Fragments arranged by size: 2301 2245 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2075 2499 4857 4942 6013 2075 Size: 424 2358 85 1071 2858 Fragments arranged by size: 2858 2358 1071 424 85 BsmI GAATG_Cn' Cuts at: 2388 3061 4307 4400 2388 Size: 673 1246 93 4784 Fragments arranged by size: 4784 1246 673 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2481 2499 4031 4115 4857 4942 Size: 399 285 406 18 1532 84 742 85 Cuts at: 4942 6013 1391 Size: 1071 2174 Fragments arranged by size: 2174 1532 1071 742 406 399 285 85 84 18 BspEI T'CCGG_A Cuts at: 6459 6459 Size: 6796 BspHI T'CATG_A Cuts at: 2253 4685 6415 2253 Size: 2432 1730 2634 Fragments arranged by size: 2634 2432 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3105 4151 844 Size: 2261 1046 3489 Fragments arranged by size: 3489 2261 1046 BsrBI CCG'CTC Cuts at: 1394 1823 4683 1394 Size: 429 2860 3507 Fragments arranged by size: 3507 2860 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2230 5291 5465 65 Size: 1183 982 3061 174 1396 Fragments arranged by size: 3061 1396 1183 982 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5440 5627 1700 Size: 110 3630 187 2869 Fragments arranged by size: 3630 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6796 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6796 BssSI C'ACGA_G Cuts at: 4856 5868 4856 Size: 1012 5784 BstAPI GCAn_nnn'nTGC Cuts at: 794 2874 794 Size: 2080 4716 BstBI TT'CG_AA Cuts at: 2007 5600 2007 Size: 3593 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6796 BstXI CCAn_nnnn'nTGG Cuts at: 2173 2488 3796 2173 Size: 315 1308 5173 Fragments arranged by size: 5173 1308 315 BstYI r'GATC_y Cuts at: 1972 2241 2296 2532 3819 4126 4493 4878 4895 Size: 269 55 236 1287 307 367 385 17 Cuts at: 4895 6347 6433 6445 6791 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1287 385 367 346 307 269 236 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2566 3261 513 Size: 552 957 544 695 4048 Fragments arranged by size: 4048 957 695 552 544 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2919 487 Size: 683 56 1693 4364 Fragments arranged by size: 4364 1693 683 56 BtsI GCAGTG_nn' Cuts at: 2757 3688 3695 3756 4312 5106 5126 2757 Size: 931 7 61 556 794 20 4427 Fragments arranged by size: 4427 931 794 556 61 20 7 ClaI AT'CG_AT Cuts at: 1554 2603 1554 Size: 1049 5747 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3583 3618 529 Size: 35 3019 35 3707 Fragments arranged by size: 3707 3019 35 35 DraI TTT'AAA Cuts at: 4100 4100 Size: 6796 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3263 5803 809 Size: 507 1947 2540 1802 Fragments arranged by size: 2540 1947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4243 5132 6761 Size: 54 1425 50 275 18 2413 889 1629 Cuts at: 6761 8 Size: 43 Fragments arranged by size: 2413 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4243 1537 Size: 275 2431 4090 Fragments arranged by size: 4090 2431 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2407 3814 4056 4726 1353 Size: 1054 1407 242 670 3423 Fragments arranged by size: 3423 1407 1054 670 242 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5383 5757 5903 387 Size: 1064 138 3794 374 146 1280 Fragments arranged by size: 3794 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2291 2495 2531 2597 2705 2799 Size: 393 600 59 204 36 66 108 94 Cuts at: 2799 3150 3182 3245 3771 4859 5456 6243 6511 Size: 351 32 63 526 1088 597 787 268 Cuts at: 6511 1239 Size: 1524 Fragments arranged by size: 1524 1088 787 600 597 526 393 351 268 204 108 94 66 63 59 36 32 EcoO109I rG'GnC_Cy Cuts at: 3906 3906 Size: 6796 EcoRI G'AATT_C Cuts at: 4105 4483 4105 Size: 378 6418 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3553 3553 Size: 6796 FspI TGC'GCA Cuts at: 5302 5302 Size: 6796 HaeII r_GCGC'y Cuts at: 1678 3671 5943 1678 Size: 1993 2272 2531 Fragments arranged by size: 2531 2272 1993 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2226 2258 2504 2536 2788 Size: 32 135 32 654 32 246 32 252 Cuts at: 2788 2820 3508 3540 3670 3702 3890 3922 5437 Size: 32 688 32 130 32 188 32 1515 Cuts at: 5437 5469 5511 5543 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1515 688 654 252 246 188 135 130 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2345 4140 4386 669 Size: 988 281 407 1795 246 3079 Fragments arranged by size: 3079 1795 988 407 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6796 HpaI GTT'AAC Cuts at: 4386 4386 Size: 6796 KasI G'GCGC_C Cuts at: 3667 3667 Size: 6796 KpnI G_GTAC'C Cuts at: 3221 4121 3221 Size: 900 5896 MfeI C'AATT_G Cuts at: 3610 4395 3610 Size: 785 6011 MluI A'CGCG_T Cuts at: 6672 6672 Size: 6796 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2612 2886 5910 6094 1305 Size: 1307 274 3024 184 2007 Fragments arranged by size: 3024 2007 1307 274 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4974 Fragments arranged by size: 4974 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2744 3324 4005 4754 5113 5272 Size: 648 918 660 580 681 749 359 159 Cuts at: 5272 518 Size: 2042 Fragments arranged by size: 2042 918 749 681 660 648 580 359 159 MspA1I CmG'CkG Cuts at: 1606 4212 4889 6037 6282 1606 Size: 2606 677 1148 245 2120 Fragments arranged by size: 2606 2120 1148 677 245 NarI GG'CG_CC Cuts at: 3668 3668 Size: 6796 NcoI C'CATG_G Cuts at: 513 1065 2022 2566 513 Size: 552 957 544 4743 Fragments arranged by size: 4743 957 552 544 NdeI CA'TA_TG Cuts at: 387 387 Size: 6796 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6796 NotI GC'GGCC_GC Cuts at: 4243 4243 Size: 6796 NspI r_CATG'y Cuts at: 1132 1229 2197 4004 4154 5699 1132 Size: 97 968 1807 150 1545 2229 Fragments arranged by size: 2229 1807 1545 968 150 97 PasI CC'CwG_GG Cuts at: 1348 3993 1348 Size: 2645 4151 PciI A'CATG_T Cuts at: 5695 5695 Size: 6796 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 1108 Size: 1536 5260 PmeI GTTT'AAAC Cuts at: 4100 4100 Size: 6796 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4865 4897 6378 6410 4865 Size: 32 1481 32 5251 Fragments arranged by size: 5251 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3906 3906 Size: 6796 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6796 PsiI TTA'TAA Cuts at: 4366 4366 Size: 6796 PstI C_TGCA'G Cuts at: 830 2284 3054 3653 4148 830 Size: 1454 770 599 495 3478 Fragments arranged by size: 3478 1454 770 599 495 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6796 SalI G'TCGA_C Cuts at: 1936 4138 1936 Size: 2202 4594 SanDI GG'GwC_CC Cuts at: 3906 3906 Size: 6796 SapI GCTCTTCn'nnn_ Cuts at: 3814 3814 Size: 6796 SbfI CC_TGCA'GG Cuts at: 3653 4148 3653 Size: 495 6301 ScaI AGT'ACT Cuts at: 5044 6756 5044 Size: 1712 5084 SfcI C'TryA_G Cuts at: 826 1045 2280 3050 3649 3833 4144 5279 5960 Size: 219 1235 770 599 184 311 1135 681 Cuts at: 5960 6151 826 Size: 191 1471 Fragments arranged by size: 1471 1235 1135 770 681 599 311 219 191 184 SfoI GGC'GCC Cuts at: 3669 3669 Size: 6796 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6796 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6796 SmaI CCC'GGG Cuts at: 1872 3665 4123 1872 Size: 1793 458 4545 Fragments arranged by size: 4545 1793 458 SmlI C'TyrA_G Cuts at: 820 1017 1943 3527 3676 4900 5801 6063 820 Size: 197 926 1584 149 1224 901 262 1553 Fragments arranged by size: 1584 1553 1224 926 901 262 197 149 SnaBI TAC'GTA Cuts at: 493 4093 4587 493 Size: 3600 494 2702 Fragments arranged by size: 3600 2702 494 SpeI A'CTAG_T Cuts at: 152 3040 152 Size: 2888 3908 SphI G_CATG'C Cuts at: 1132 4004 4154 1132 Size: 2872 150 3774 Fragments arranged by size: 3774 2872 150 SrfI GCCC'GGGC Cuts at: 3665 3665 Size: 6796 SspI AAT'ATT Cuts at: 5 52 4720 5 Size: 47 4668 2081 Fragments arranged by size: 4668 2081 47 StuI AGG'CCT Cuts at: 1517 2763 2891 1517 Size: 1246 128 5422 Fragments arranged by size: 5422 1246 128 StyI C'CwwG_G Cuts at: 513 1065 2022 2038 2566 2592 2926 4235 513 Size: 552 957 16 528 26 334 1309 3074 Fragments arranged by size: 3074 1309 957 552 528 334 26 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3875 4823 4840 4993 5178 5623 325 Size: 1255 2295 948 17 153 185 445 1498 Fragments arranged by size: 2295 1498 1255 948 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2932 5042 6754 Size: 274 80 33 51 1443 955 2110 1712 Cuts at: 6754 96 Size: 138 Fragments arranged by size: 2110 1712 1443 955 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3005 3037 6756 6788 3005 Size: 32 3719 32 3013 Fragments arranged by size: 3719 3013 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6796 XbaI T'CTAG_A Cuts at: 4132 4132 Size: 6796 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3211 3211 Size: 6796 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6796 XmaI C'CCGG_G Cuts at: 1870 3663 4121 1870 Size: 1793 458 4545 Fragments arranged by size: 4545 1793 458 XmnI GAAnn'nnTTC Cuts at: 2184 4925 2184 Size: 2741 4055 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4604 276 Size: 53 83 186 936 3070 2468 Fragments arranged by size: 3070 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SwaI