(Circular) (Six-base) MAPSORT of: pFN21ASDA0813.seq Check: 6415 from: 1 to: 9331 pFN21ASDA0813 9331 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2147 2852 2891 4284 4847 4964 5114 5240 2147 Size: 705 39 1393 563 117 150 126 6238 Fragments arranged by size: 6238 1393 705 563 150 126 117 39 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7141 278 Size: 53 83 186 936 5605 2468 Fragments arranged by size: 5605 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4364 6674 1937 Size: 2427 2310 4594 Fragments arranged by size: 4594 2427 2310 Acc65I G'GTAC_C Cuts at: 6652 6652 Size: 9331 AclI AA'CG_TT Cuts at: 1563 7458 7831 1563 Size: 5895 373 3063 Fragments arranged by size: 5895 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3629 3743 4156 6551 7394 8778 9046 1632 Size: 1997 114 413 2395 843 1384 268 1917 Fragments arranged by size: 2395 1997 1917 1384 843 413 268 114 AflII C'TTAA_G Cuts at: 820 1017 4417 820 Size: 197 3400 5734 Fragments arranged by size: 5734 3400 197 AflIII A'CryG_T Cuts at: 1203 2388 8230 9207 1203 Size: 1185 5842 977 1327 Fragments arranged by size: 5842 1327 1185 977 AgeI A'CCGG_T Cuts at: 8162 8162 Size: 9331 AhdI GACnn_n'nnGTC Cuts at: 8060 8060 Size: 9331 AleI CACnn'nnGTG Cuts at: 1166 4231 6177 1166 Size: 3065 1946 4320 Fragments arranged by size: 4320 3065 1946 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2192 3828 6301 6311 1184 Size: 1008 1636 2473 10 4204 Fragments arranged by size: 4204 2473 1636 1008 10 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2390 2391 2422 2423 4970 5002 2390 Size: 1 31 1 2547 32 6719 Fragments arranged by size: 6719 2547 32 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2710 3178 3232 3322 3791 6304 7074 8646 2710 Size: 468 54 90 469 2513 770 1572 3395 Fragments arranged by size: 3395 2513 1572 770 469 468 90 54 ApaI G_GGCC'C Cuts at: 2756 4285 4710 5223 5311 6102 6274 2756 Size: 1529 425 513 88 791 172 5813 Fragments arranged by size: 5813 1529 791 513 425 172 88 ApaLI G'TGCA_C Cuts at: 2878 7388 8544 2878 Size: 4510 1156 3665 Fragments arranged by size: 4510 3665 1156 ApoI r'AATT_y Cuts at: 1493 2503 6640 6866 7018 9127 1493 Size: 1010 4137 226 152 2109 1697 Fragments arranged by size: 4137 2109 1697 1010 226 152 AseI AT'TA_AT Cuts at: 160 1032 7885 160 Size: 872 6853 1606 Fragments arranged by size: 6853 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9331 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2749 2945 6244 6656 1870 Size: 73 93 713 196 3299 412 4545 Fragments arranged by size: 4545 3299 713 412 196 93 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3960 3993 3960 Size: 33 9298 BamHI G'GATC_C Cuts at: 2247 5625 6661 7028 2247 Size: 3378 1036 367 4550 Fragments arranged by size: 4550 3378 1036 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2103 2111 3410 3432 4613 Size: 325 217 627 316 8 1299 22 1181 Cuts at: 4613 4835 4952 5207 5869 5942 6028 6329 6652 Size: 222 117 255 662 73 86 301 323 Cuts at: 6652 8107 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1299 1181 662 627 325 323 316 301 255 222 217 117 86 73 22 8 BanII G_rGCy'C Cuts at: 1391 2076 2274 2736 2756 4285 4710 5223 5311 Size: 685 198 462 20 1529 425 513 88 Cuts at: 5311 5411 5822 6102 6274 6285 6494 6650 1391 Size: 100 411 280 172 11 209 156 4072 Fragments arranged by size: 4072 1529 685 513 462 425 411 280 209 198 172 156 100 88 20 11 BbeI G_GCGC'C Cuts at: 2107 3436 5873 2107 Size: 1329 2437 5565 Fragments arranged by size: 5565 2437 1329 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4673 928 Size: 971 2774 5586 Fragments arranged by size: 5586 2774 971 BbvCI CC'TCA_GC Cuts at: 2711 4466 5352 2711 Size: 1755 886 6690 Fragments arranged by size: 6690 1755 886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2878 2912 5639 5673 7522 7556 1903 Size: 34 941 34 2727 34 1849 34 3678 Fragments arranged by size: 3678 2727 1849 941 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3988 7223 8433 3988 Size: 3235 1210 4886 Fragments arranged by size: 4886 3235 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9331 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2065 2459 2769 Size: 107 122 71 631 605 393 394 310 Cuts at: 2769 2933 4884 5487 6069 6309 7942 136 Size: 164 1951 603 582 240 1633 1525 Fragments arranged by size: 1951 1633 1525 631 605 603 582 394 393 310 240 164 122 107 71 BglII A'GATC_T Cuts at: 9326 9326 Size: 9331 BlpI GC'TnA_GC Cuts at: 3484 6748 3484 Size: 3264 6067 Bme1580I G_kGCm'C Cuts at: 1790 2126 2756 2841 2882 3293 3415 4226 4285 Size: 336 630 85 41 411 122 811 59 Cuts at: 4285 4710 4838 4955 5117 5210 5223 5311 6102 Size: 425 128 117 162 93 13 88 791 Cuts at: 6102 6201 6253 6274 6343 6500 6616 7392 8548 Size: 99 52 21 69 157 116 776 1156 Cuts at: 8548 1790 Size: 2573 Fragments arranged by size: 2573 1156 811 791 776 630 425 411 336 162 157 128 122 117 116 99 93 88 85 69 59 52 41 21 13 BmgBI CAC'GTC Cuts at: 1318 3553 4156 4585 1318 Size: 2235 603 429 6064 Fragments arranged by size: 6064 2235 603 429 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2737 3634 3705 Size: 416 53 83 399 24 1320 897 71 Cuts at: 3705 5191 5305 7062 8020 442 Size: 1486 114 1757 958 1753 Fragments arranged by size: 1757 1753 1486 1320 958 897 416 399 114 83 71 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9331 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2375 2591 2990 3021 3586 3707 3845 3986 Size: 1136 216 399 31 565 121 138 141 Cuts at: 3986 7991 1239 Size: 4005 2579 Fragments arranged by size: 4005 2579 1136 565 399 216 141 138 121 31 Bpu10I CC'TnA_GC Cuts at: 2711 2812 3041 3627 4394 4466 5352 2711 Size: 101 229 586 767 72 886 6690 Fragments arranged by size: 6690 886 767 586 229 101 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2258 2461 3347 3886 4877 7456 8321 8619 2258 Size: 203 886 539 991 2579 865 298 2970 Fragments arranged by size: 2970 2579 991 886 865 539 298 203 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2942 5608 7994 882 Size: 617 1443 2666 2386 2219 Fragments arranged by size: 2666 2386 2219 1443 617 BsaAI yAC'GTr Cuts at: 493 1204 4992 6628 7122 493 Size: 711 3788 1636 494 2702 Fragments arranged by size: 3788 2702 1636 711 494 BsaBI GATnn'nnATC Cuts at: 3680 9325 3680 Size: 5645 3686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2104 3075 3433 5076 Size: 53 83 186 936 571 971 358 1643 Cuts at: 5076 5870 7138 7520 8155 275 Size: 794 1268 382 635 1451 Fragments arranged by size: 1643 1451 1268 971 936 794 635 571 382 358 186 83 53 BsaWI w'CCGG_w Cuts at: 2144 2395 3477 3834 5167 7764 8162 8436 8583 Size: 251 1082 357 1333 2597 398 274 147 Cuts at: 8583 8994 2144 Size: 411 2481 Fragments arranged by size: 2597 2481 1333 1082 411 398 357 274 251 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4008 4038 4574 4604 5321 Size: 30 165 30 2605 30 536 30 717 Cuts at: 5321 5351 1178 Size: 30 5158 Fragments arranged by size: 5158 2605 717 536 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3935 4208 4724 6596 1186 Size: 2749 273 516 1872 3921 Fragments arranged by size: 3921 2749 1872 516 273 BseYI C'CCAG_C Cuts at: 2755 4659 4703 4880 5714 6123 8534 9030 2755 Size: 1904 44 177 834 409 2411 496 3056 Fragments arranged by size: 3056 2411 1904 834 496 409 177 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2119 2125 2369 2642 2830 3308 3788 Size: 573 383 6 244 273 188 478 480 Cuts at: 3788 6119 6577 1163 Size: 2331 458 3917 Fragments arranged by size: 3917 2331 573 480 478 458 383 273 244 188 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2069 6781 7542 8570 1540 Size: 275 186 68 4712 761 1028 2301 Fragments arranged by size: 4712 2301 1028 761 275 186 68 BsiHKAI G_wGCw'C Cuts at: 2076 2560 2750 2882 3485 3602 4997 5165 7392 Size: 484 190 132 603 117 1395 168 2227 Cuts at: 7392 7477 8548 2076 Size: 85 1071 2859 Fragments arranged by size: 2859 2227 1395 1071 603 484 190 168 132 117 85 BsmI GAATG_Cn' Cuts at: 3193 4296 4766 6842 6935 3193 Size: 1103 470 2076 93 5589 Fragments arranged by size: 5589 2076 1103 470 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3395 5732 3395 Size: 2337 6994 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2126 2274 2560 2736 2750 2756 Size: 399 286 50 148 286 176 14 6 Cuts at: 2756 2841 2882 3293 3415 3485 3602 4226 4285 Size: 85 41 411 122 70 117 624 59 Cuts at: 4285 4710 4838 4955 4997 5117 5165 5210 5223 Size: 425 128 117 42 120 48 45 13 Cuts at: 5223 5311 5411 5822 6102 6201 6253 6274 6285 Size: 88 100 411 280 99 52 21 11 Cuts at: 6285 6343 6494 6500 6616 6650 7392 7477 8548 Size: 58 151 6 116 34 742 85 1071 Cuts at: 8548 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 624 425 411 411 399 286 286 280 176 151 148 128 122 120 117 117 116 100 99 88 85 85 70 59 58 52 50 48 45 42 41 34 21 14 13 11 6 6 BspEI T'CCGG_A Cuts at: 3834 5167 8994 3834 Size: 1333 3827 4171 Fragments arranged by size: 4171 3827 1333 BspHI T'CATG_A Cuts at: 7220 8950 7220 Size: 1730 7601 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2147 2852 2891 2949 3240 4284 4386 4847 Size: 1303 705 39 58 291 1044 102 461 Cuts at: 4847 4964 5114 5240 5559 5574 5590 6686 844 Size: 117 150 126 319 15 16 1096 3489 Fragments arranged by size: 3489 1303 1096 1044 705 461 319 291 150 126 117 102 58 39 16 15 BsrBI CCG'CTC Cuts at: 1394 1823 2359 3093 3840 4518 7218 1394 Size: 429 536 734 747 678 2700 3507 Fragments arranged by size: 3507 2700 747 734 678 536 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2896 4552 4689 5655 6197 7826 8000 Size: 1183 1648 1656 137 966 542 1629 174 Cuts at: 8000 65 Size: 1396 Fragments arranged by size: 1656 1648 1629 1396 1183 966 542 174 137 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 5006 5945 7975 8162 1700 Size: 110 249 2947 939 2030 187 2869 Fragments arranged by size: 2947 2869 2030 939 249 187 110 BsrGI T'GTAC_A Cuts at: 96 4863 5634 5973 96 Size: 4767 771 339 3454 Fragments arranged by size: 4767 3454 771 339 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9331 BssSI C'ACGA_G Cuts at: 4385 5189 6493 7391 8403 4385 Size: 804 1304 898 1012 5313 Fragments arranged by size: 5313 1304 1012 898 804 BstAPI GCAn_nnn'nTGC Cuts at: 794 3900 6196 6304 794 Size: 3106 2296 108 3821 Fragments arranged by size: 3821 3106 2296 108 BstBI TT'CG_AA Cuts at: 2007 8135 2007 Size: 6128 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9331 BstXI CCAn_nnnn'nTGG Cuts at: 3592 3823 5449 5830 3592 Size: 231 1626 381 7093 Fragments arranged by size: 7093 1626 381 231 BstYI r'GATC_y Cuts at: 1972 2247 3270 3621 5170 5379 5625 5931 6661 Size: 275 1023 351 1549 209 246 306 730 Cuts at: 6661 7028 7413 7430 8882 8968 8980 9326 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1549 1452 1023 730 385 367 351 346 306 275 246 209 86 17 12 Bsu36I CC'TnA_GG Cuts at: 6325 6325 Size: 9331 BtgI C'CryG_G Cuts at: 513 1065 2022 2151 2164 3142 3645 4251 4540 Size: 552 957 129 13 978 503 606 289 Cuts at: 4540 5579 5607 5829 5895 5953 6166 6184 513 Size: 1039 28 222 66 58 213 18 3660 Fragments arranged by size: 3660 1039 978 957 606 552 503 289 222 213 129 66 58 28 18 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4126 6576 487 Size: 683 56 2900 2450 3242 Fragments arranged by size: 3242 2900 2450 683 56 BtsI GCAGTG_nn' Cuts at: 2711 2922 3245 4490 5269 6109 6305 6313 6847 Size: 211 323 1245 779 840 196 8 534 Cuts at: 6847 7641 7661 2711 Size: 794 20 4381 Fragments arranged by size: 4381 1245 840 794 779 534 323 211 196 20 8 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9331 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2657 2692 4993 5028 529 Size: 35 2093 35 2301 35 4832 Fragments arranged by size: 4832 2301 2093 35 35 35 DraI TTT'AAA Cuts at: 3706 6635 3706 Size: 2929 6402 DraIII CAC_nnn'GTG Cuts at: 2745 6347 6349 6603 2745 Size: 3602 2 254 5473 Fragments arranged by size: 5473 3602 254 2 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4253 4594 8338 809 Size: 507 2937 341 3744 1802 Fragments arranged by size: 3744 2937 1802 507 341 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2066 2720 2871 Size: 54 1425 50 275 18 236 654 151 Cuts at: 2871 2934 4029 4605 5576 6169 6310 6778 7667 Size: 63 1095 576 971 593 141 468 889 Cuts at: 7667 9296 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1095 971 889 654 593 576 468 275 236 151 141 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2066 6778 1537 Size: 275 254 4712 4090 Fragments arranged by size: 4712 4090 275 254 EarI CTCTTCn'nnn_ Cuts at: 1353 2741 2789 7261 1353 Size: 1388 48 4472 3423 Fragments arranged by size: 4472 3423 1388 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2642 5909 7918 8292 8438 387 Size: 1064 138 1053 3267 2009 374 146 1280 Fragments arranged by size: 3267 2009 1280 1064 1053 374 146 138 EcoICRI GAG'CTC Cuts at: 2074 2074 Size: 9331 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2375 2591 2990 3021 3586 3629 3707 Size: 393 743 216 399 31 565 43 78 Cuts at: 3707 3743 3845 3986 4156 6551 7394 7991 8778 Size: 36 102 141 170 2395 843 597 787 Cuts at: 8778 9046 1239 Size: 268 1524 Fragments arranged by size: 2395 1524 843 787 743 597 565 399 393 268 216 170 141 102 78 43 36 31 EcoNI CCTnn'n_nnAGG Cuts at: 3284 4289 4565 3284 Size: 1005 276 8050 Fragments arranged by size: 8050 1005 276 EcoO109I rG'GnC_Cy Cuts at: 2046 2547 3085 3229 3771 3870 4282 4707 4769 Size: 501 538 144 542 99 412 425 62 Cuts at: 4769 5307 6098 6270 6271 6406 6520 6567 2046 Size: 538 791 172 1 135 114 47 4810 Fragments arranged by size: 4810 791 542 538 538 501 425 412 172 144 135 114 99 62 47 1 EcoRI G'AATT_C Cuts at: 6640 7018 6640 Size: 378 8953 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2278 6565 2278 Size: 4287 5044 FspI TGC'GCA Cuts at: 3386 3932 6622 7837 3386 Size: 546 2690 1215 4880 Fragments arranged by size: 4880 2690 1215 546 HaeII r_GCGC'y Cuts at: 1678 2030 2107 2218 3436 5873 8478 1678 Size: 352 77 111 1218 2437 2605 2531 Fragments arranged by size: 2605 2531 2437 1218 352 111 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3034 3066 4574 4606 5147 Size: 32 135 32 1462 32 1508 32 541 Cuts at: 5147 5179 5782 5814 5891 5923 6227 6259 6374 Size: 32 603 32 77 32 304 32 115 Cuts at: 6374 6406 6554 6586 7972 8004 8046 8078 1373 Size: 32 148 32 1386 32 42 32 2626 Fragments arranged by size: 2626 1508 1462 1386 603 541 304 148 135 115 77 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5186 5329 5527 5905 6675 6921 Size: 988 281 3248 143 198 378 770 246 Cuts at: 6921 669 Size: 3079 Fragments arranged by size: 3248 3079 988 770 378 281 246 198 143 HindIII A'AGCT_T Cuts at: 748 2475 748 Size: 1727 7604 HpaI GTT'AAC Cuts at: 6921 6921 Size: 9331 KasI G'GCGC_C Cuts at: 2103 3432 5869 2103 Size: 1329 2437 5565 Fragments arranged by size: 5565 2437 1329 KpnI G_GTAC'C Cuts at: 6656 6656 Size: 9331 MfeI C'AATT_G Cuts at: 6930 6930 Size: 9331 MluI A'CGCG_T Cuts at: 9207 9207 Size: 9331 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2688 4799 8445 8629 1305 Size: 1383 2111 3646 184 2007 Fragments arranged by size: 3646 2111 2007 1383 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4031 4607 5578 6171 6312 Size: 54 1425 343 2199 576 971 593 141 Cuts at: 6312 10 Size: 3029 Fragments arranged by size: 3029 2199 1425 971 593 576 343 141 54 MslI CAynn'nnrTG Cuts at: 518 1166 2256 2612 3698 4231 4439 4545 5061 Size: 648 1090 356 1086 533 208 106 516 Cuts at: 5061 5685 5828 6177 7289 7648 7807 518 Size: 624 143 349 1112 359 159 2042 Fragments arranged by size: 2042 1112 1090 1086 648 624 533 516 359 356 349 208 159 143 106 MspA1I CmG'CkG Cuts at: 1606 2281 2296 2383 2512 2532 2719 2808 3417 Size: 675 15 87 129 20 187 89 609 Cuts at: 3417 3529 3576 3823 3900 4057 4360 4450 4647 Size: 112 47 247 77 157 303 90 197 Cuts at: 4647 4659 4884 4923 5149 5295 5912 6152 6222 Size: 12 225 39 226 146 617 240 70 Cuts at: 6222 6747 7424 8572 8817 1606 Size: 525 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 675 617 609 525 303 247 245 240 226 225 197 187 157 146 129 112 90 89 87 77 70 47 39 20 15 12 NarI GG'CG_CC Cuts at: 2104 3433 5870 2104 Size: 1329 2437 5565 Fragments arranged by size: 5565 2437 1329 NcoI C'CATG_G Cuts at: 513 1065 2022 5829 6166 6184 513 Size: 552 957 3807 337 18 3660 Fragments arranged by size: 3807 3660 957 552 337 18 NdeI CA'TA_TG Cuts at: 387 2631 387 Size: 2244 7087 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9331 NotI GC'GGCC_GC Cuts at: 6778 6778 Size: 9331 NspI r_CATG'y Cuts at: 1132 1229 2392 3847 5239 6689 8234 1132 Size: 97 1163 1455 1392 1450 1545 2229 Fragments arranged by size: 2229 1545 1455 1450 1392 1163 97 PasI CC'CwG_GG Cuts at: 1348 6095 1348 Size: 4747 4584 PciI A'CATG_T Cuts at: 2388 8230 2388 Size: 5842 3489 PflMI CCAn_nnn'nTGG Cuts at: 1108 4717 5027 5203 5317 1108 Size: 3609 310 176 114 5122 Fragments arranged by size: 5122 3609 310 176 114 PfoI T'CCnGG_A Cuts at: 2998 3225 3684 4351 6414 2998 Size: 227 459 667 2063 5915 Fragments arranged by size: 5915 2063 667 459 227 PmeI GTTT'AAAC Cuts at: 6635 6635 Size: 9331 PmlI CAC'GTG Cuts at: 4992 4992 Size: 9331 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7400 7432 8913 8945 7400 Size: 32 1481 32 7786 Fragments arranged by size: 7786 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2046 3229 3870 4769 6406 6567 2046 Size: 1183 641 899 1637 161 4810 Fragments arranged by size: 4810 1637 1183 899 641 161 PshAI GACnn'nnGTC Cuts at: 1613 4360 1613 Size: 2747 6584 PsiI TTA'TAA Cuts at: 6901 6901 Size: 9331 PspOMI G'GGCC_C Cuts at: 2752 4281 4706 5219 5307 6098 6270 2752 Size: 1529 425 513 88 791 172 5813 Fragments arranged by size: 5813 1529 791 513 425 172 88 PstI C_TGCA'G Cuts at: 830 2181 2295 2888 3237 4152 4383 4485 5124 Size: 1351 114 593 349 915 231 102 639 Cuts at: 5124 5264 5285 5556 5994 6290 6300 6683 830 Size: 140 21 271 438 296 10 383 3478 Fragments arranged by size: 3478 1351 915 639 593 438 383 349 296 271 231 140 114 102 21 10 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9331 PvuII CAG'CTG Cuts at: 2281 2296 2383 2512 2532 2808 3529 3823 3900 Size: 15 87 129 20 276 721 294 77 Cuts at: 3900 4057 4360 4450 4659 4923 5149 5912 6222 Size: 157 303 90 209 264 226 763 310 Cuts at: 6222 2281 Size: 5390 Fragments arranged by size: 5390 763 721 310 303 294 276 264 226 209 157 129 90 87 77 20 15 RsrII CG'GwC_CG Cuts at: 2062 2062 Size: 9331 SacI G_AGCT'C Cuts at: 2076 2076 Size: 9331 SalI G'TCGA_C Cuts at: 1936 6673 1936 Size: 4737 4594 SanDI GG'GwC_CC Cuts at: 6406 6406 Size: 9331 SapI GCTCTTCn'nnn_ Cuts at: 2741 2741 Size: 9331 SbfI CC_TGCA'GG Cuts at: 4152 6683 4152 Size: 2531 6800 ScaI AGT'ACT Cuts at: 3500 7579 9291 3500 Size: 4079 1712 3540 Fragments arranged by size: 4079 3540 1712 SexAI A'CCwGG_T Cuts at: 6426 6426 Size: 9331 SfcI C'TryA_G Cuts at: 826 1045 2177 2291 2884 3016 3170 3233 4148 Size: 219 1132 114 593 132 154 63 915 Cuts at: 4148 4379 4481 5120 5260 5281 5359 5552 5728 Size: 231 102 639 140 21 78 193 176 Cuts at: 5728 5990 6217 6286 6296 6679 7814 8495 8686 Size: 262 227 69 10 383 1135 681 191 Cuts at: 8686 826 Size: 1471 Fragments arranged by size: 1471 1135 1132 915 681 639 593 383 262 231 227 219 193 191 176 154 140 132 114 102 78 69 63 21 10 SfiI GGCCn_nnn'nGGCC Cuts at: 2065 2065 Size: 9331 SfoI GGC'GCC Cuts at: 2105 3434 5871 2105 Size: 1329 2437 5565 Fragments arranged by size: 5565 2437 1329 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9331 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9331 SmaI CCC'GGG Cuts at: 1872 2038 6658 1872 Size: 166 4620 4545 Fragments arranged by size: 4620 4545 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 2273 2440 3362 3865 4417 4856 Size: 197 926 330 167 922 503 552 439 Cuts at: 4856 7435 8336 8598 820 Size: 2579 901 262 1553 Fragments arranged by size: 2579 1553 926 922 901 552 503 439 330 262 197 167 SnaBI TAC'GTA Cuts at: 493 6628 7122 493 Size: 6135 494 2702 Fragments arranged by size: 6135 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9331 SphI G_CATG'C Cuts at: 1132 5239 6689 1132 Size: 4107 1450 3774 Fragments arranged by size: 4107 3774 1450 SspI AAT'ATT Cuts at: 5 52 7255 5 Size: 47 7203 2081 Fragments arranged by size: 7203 2081 47 StuI AGG'CCT Cuts at: 1517 2919 3159 5138 5467 6294 6323 1517 Size: 1402 240 1979 329 827 29 4525 Fragments arranged by size: 4525 1979 1402 827 329 240 29 StyI C'CwwG_G Cuts at: 513 1065 2022 3285 4290 4765 5829 6166 6184 Size: 552 957 1263 1005 475 1064 337 18 Cuts at: 6184 6333 6770 513 Size: 149 437 3074 Fragments arranged by size: 3074 1263 1064 1005 957 552 475 437 337 149 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2099 2756 4651 6115 6466 7358 7375 Size: 1255 519 657 1895 1464 351 892 17 Cuts at: 7375 7528 7713 8158 325 Size: 153 185 445 1498 Fragments arranged by size: 1895 1498 1464 1255 892 657 519 445 351 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3498 4863 5634 Size: 274 80 33 51 1443 1521 1365 771 Cuts at: 5634 5973 7577 9289 96 Size: 339 1604 1712 138 Fragments arranged by size: 1712 1604 1521 1443 1365 771 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2139 2171 4008 4040 4333 4365 9291 9323 2139 Size: 32 1837 32 293 32 4926 32 2147 Fragments arranged by size: 4926 2147 1837 293 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3554 6410 1316 Size: 2238 2856 4237 Fragments arranged by size: 4237 2856 2238 XbaI T'CTAG_A Cuts at: 6667 6667 Size: 9331 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6116 6233 6116 Size: 117 9214 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9331 XmaI C'CCGG_G Cuts at: 1870 2036 6656 1870 Size: 166 4620 4545 Fragments arranged by size: 4620 4545 166 XmnI GAAnn'nnTTC Cuts at: 3341 4337 7460 3341 Size: 996 3123 5212 Fragments arranged by size: 5212 3123 996 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7139 276 Size: 53 83 186 936 5605 2468 Fragments arranged by size: 5605 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI SacII SrfI SwaI