Restriction map for
pFN21ASDA0797
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BglI BglII BlnI BsaHI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsoBI BspDI BspEI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstYI Bsu36I ClaI
CpoI DraIII DrdI DsaI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MflI MluI MluNI MroI MscI
MspA1I MvnI NarI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI PvuII RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AflII 2721 CTTAAG C^TTAAG
AflIII 326 ACATGT A^C[GA][CT]GT
AlwNI 3081 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 3045 GGGCTC G[GA]GC[CT]^C
BbvCI 1057 CCTCAGC CCTCAGC(-5/-2)
BciVI 2915 GTATCC GTATCC(6/5)
BclI 108 TGATCA T^GATCA
BfrI 2721 CTTAAG C^TTAAG
BlpI 1548 GCTGAGC GC^T[ACGT]AGC
BmyI 3045 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 1548 GCTGAGC GC^T[ACGT]AGC
BsaAI 2239 CACGTA [CT]AC^GT[GA]
BsaBI 2512 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsmFI 148 GGGAC GGGAC(10/14)
BsmI 1160 GCATTC GAATGC(1/-1)
Bsp1286I 3045 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 1783 TGTACA T^GTACA
BspLU11I 326 ACATGT A^CATGT
BspMI 2015 ACCTGC ACCTGC(4/8)
BsrBI 15 GAGCGG CCGCTC(-3/-3)
BsrFI 7 GCCGGC [GA]^CCGG[CT]
BsrGI 1783 TGTACA T^GTACA
Bst1107I 1346 GTATAC GTA^TAC
BstXI 2423 CCAGTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1346 GTATAC GTA^TAC
CelII 1548 GCTGAGC GC^T[ACGT]AGC
CfoI 26 GCGC GCG^C
Cfr10I 7 GCCGGC [GA]^CCGG[CT]
EaeI 5 CGGCCG [CT]^GGCC[GA]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoT14I 540 CCAAGG C^C[AT][AT]GG
FbaI 108 TGATCA T^GATCA
HgaI 2107 GACGC GACGC(5/10)
HhaI 26 GCGC GCG^C
HinP1I 26 GCGC G^CGC
HincII 2791 GTCGAC GT[CT]^[GA]AC
HindII 2791 GTCGAC GT[CT]^[GA]AC
HindIII 50 AAGCTT A^AGCTT
MamI 2512 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 7 GCCGGC GCC^GGC
NgoMIV 7 GCCGGC G^CCGGC
PflFI 1321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1093 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2012 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 785 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2859 CTGCAG CTGCA^G
SalI 2791 GTCGAC G^TCGAC
SspBI 1783 TGTACA T^GTACA
StyI 540 CCAAGG C^C[AT][AT]GG
Tth111I 1321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1093 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AseI 514 ATTAAT AT^TAAT
1699 ATTAAT
AsnI 514 ATTAAT AT^TAAT
1699 ATTAAT
Asp700I 841 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3020 GAAGTTTTTC
BcnI 2234 CCCGG CC^[GC]GG
2320 CCCGG
BfaI 59 CTAG C^TAG
1420 CTAG
BspHI 111 TCATGA T^CATGA
2175 TCATGA
BsrDI 1193 CATTGC GCAATG(2/0)
2494 CATTGC
Cac8I 7 GCCGGC GC[ACGT]^[ACGT]GC
1062 GCCTGC
DraII 2012 AGGACCT [GA]G^G[ACGT]CC[CT]
2940 AGGCCCT
EcoO109I 2012 AGGACCT [GA]G^G[ACGT]CC[CT]
2940 AGGCCCT
HphI 244 GGTGA GGTGA(8/7)
2755 TCACC
MaeI 59 CTAG C^TAG
1420 CTAG
MfeI 314 CAATTG C^AATTG
851 CAATTG
MunI 314 CAATTG C^AATTG
851 CAATTG
NciI 2234 CCCGG CC^[GC]GG
2320 CCCGG
NdeI 263 CATATG CA^TATG
2559 CATATG
NspI 326 ACATGT [GA]CATG^[CT]
1075 GCATGT
PshBI 514 ATTAAT AT^TAAT
1699 ATTAAT
RcaI 111 TCATGA T^CATGA
2175 TCATGA
SmlI 1274 CTCAAG C^T[CT][GA]AG
2721 CTTAAG
XmnI 841 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3020 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII BbsI BbvI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I