(Circular) (Six-base) MAPSORT of: pFN21ASDA0741.seq Check: 7865 from: 1 to: 8389 pFN21ASDA0741 8389 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3995 3995 Size: 8389 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6199 278 Size: 53 83 186 936 4663 2468 Fragments arranged by size: 4663 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5732 1937 Size: 3795 4594 Acc65I G'GTAC_C Cuts at: 4483 5710 4483 Size: 1227 7162 AclI AA'CG_TT Cuts at: 1563 6516 6889 1563 Size: 4953 373 3063 Fragments arranged by size: 4953 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2928 3636 5070 5681 6452 7836 8104 1632 Size: 1296 708 1434 611 771 1384 268 1917 Fragments arranged by size: 1917 1434 1384 1296 771 708 611 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8192 AflIII A'CryG_T Cuts at: 1203 3985 7288 8265 1203 Size: 2782 3303 977 1327 Fragments arranged by size: 3303 2782 1327 977 AgeI A'CCGG_T Cuts at: 7220 7220 Size: 8389 AhdI GACnn_n'nnGTC Cuts at: 7118 7118 Size: 8389 AleI CACnn'nnGTG Cuts at: 1166 4472 5395 1166 Size: 3306 923 4160 Fragments arranged by size: 4160 3306 923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8389 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3573 3605 3573 Size: 32 8357 AlwNI CAG_nnn'CTG Cuts at: 3794 6132 7704 3794 Size: 2338 1572 4479 Fragments arranged by size: 4479 2338 1572 ApaI G_GGCC'C Cuts at: 4710 4710 Size: 8389 ApaLI G'TGCA_C Cuts at: 6446 7602 6446 Size: 1156 7233 ApoI r'AATT_y Cuts at: 1493 2291 2563 2597 2846 3960 4081 4379 4412 Size: 798 272 34 249 1114 121 298 33 Cuts at: 4412 5009 5187 5270 5413 5698 5924 6076 8185 Size: 597 178 83 143 285 226 152 2109 Cuts at: 8185 1493 Size: 1697 Fragments arranged by size: 2109 1697 1114 798 597 298 285 272 249 226 178 152 143 121 83 34 33 AseI AT'TA_AT Cuts at: 160 1032 4056 6943 160 Size: 872 3024 2887 1606 Fragments arranged by size: 3024 2887 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8389 AvaI C'yCGr_G Cuts at: 1870 1943 4621 5334 5714 1870 Size: 73 2678 713 380 4545 Fragments arranged by size: 4545 2678 713 380 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2877 2910 2877 Size: 33 8356 BamHI G'GATC_C Cuts at: 5719 6086 5719 Size: 367 8022 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4483 4631 5710 7165 618 Size: 325 217 627 2696 148 1079 1455 1842 Fragments arranged by size: 2696 1842 1455 1079 627 325 217 148 BanII G_rGCy'C Cuts at: 1391 3788 3916 4227 4710 5708 1391 Size: 2397 128 311 483 998 4072 Fragments arranged by size: 4072 2397 998 483 311 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 3758 3959 5548 928 Size: 971 159 1700 201 1589 3769 Fragments arranged by size: 3769 1700 1589 971 201 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6580 6614 1903 Size: 34 4643 34 3678 Fragments arranged by size: 4643 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6281 7491 6281 Size: 1210 7179 BclI T'GATC_A Cuts at: 1548 4653 1548 Size: 3105 5284 BfrBI ATG'CAT Cuts at: 4213 4213 Size: 8389 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7000 136 Size: 107 122 71 631 605 5328 1525 Fragments arranged by size: 5328 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8384 8384 Size: 8389 BlpI GC'TnA_GC Cuts at: 3069 5806 3069 Size: 2737 5652 Bme1580I G_kGCm'C Cuts at: 1790 4634 4710 5034 6450 7606 1790 Size: 2844 76 324 1416 1156 2573 Fragments arranged by size: 2844 2573 1416 1156 324 76 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8389 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4994 5051 6120 Size: 416 53 83 399 24 3577 57 1069 Cuts at: 6120 7078 442 Size: 958 1753 Fragments arranged by size: 3577 1753 1069 958 416 399 83 57 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8389 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2910 4716 4856 7049 1239 Size: 1671 1806 140 2193 2579 Fragments arranged by size: 2579 2193 1806 1671 140 Bpu10I CC'TnA_GC Cuts at: 3306 4806 3306 Size: 1500 6889 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2214 6514 7379 7677 2214 Size: 4300 865 298 2926 Fragments arranged by size: 4300 2926 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7052 882 Size: 617 5553 2219 Fragments arranged by size: 5553 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5686 6180 493 Size: 711 4482 494 2702 Fragments arranged by size: 4482 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3813 5306 8383 3813 Size: 1493 3077 3819 Fragments arranged by size: 3819 3077 1493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2682 6196 6578 7213 Size: 53 83 186 936 1149 3514 382 635 Cuts at: 7213 275 Size: 1451 Fragments arranged by size: 3514 1451 1149 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6822 7220 7494 7641 8052 6822 Size: 398 274 147 411 7159 Fragments arranged by size: 7159 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3469 3499 4953 4983 1178 Size: 30 165 30 2066 30 1454 30 4584 Fragments arranged by size: 4584 2066 1454 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2531 3575 3635 3638 3641 3644 3686 3695 Size: 1345 1044 60 3 3 3 42 9 Cuts at: 3695 4400 4734 1186 Size: 705 334 4841 Fragments arranged by size: 4841 1345 1044 705 334 60 42 9 3 3 3 BseYI C'CCAG_C Cuts at: 7592 8088 7592 Size: 496 7893 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4479 5217 1163 Size: 573 2743 738 4335 Fragments arranged by size: 4335 2743 738 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5839 6600 7628 1540 Size: 275 186 3838 761 1028 2301 Fragments arranged by size: 3838 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3788 4597 6450 6535 7606 3788 Size: 809 1853 85 1071 4571 Fragments arranged by size: 4571 1853 1071 809 85 BsmI GAATG_Cn' Cuts at: 4119 4438 5900 5993 4119 Size: 319 1462 93 6515 Fragments arranged by size: 6515 1462 319 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3788 3916 4227 4597 4634 4710 5034 Size: 399 1998 128 311 370 37 76 324 Cuts at: 5034 5708 6450 6535 7606 1391 Size: 674 742 85 1071 2174 Fragments arranged by size: 2174 1998 1071 742 674 399 370 324 311 128 85 76 37 BspEI T'CCGG_A Cuts at: 8052 8052 Size: 8389 BspHI T'CATG_A Cuts at: 6278 8008 6278 Size: 1730 6659 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3995 5360 5744 844 Size: 3151 1365 384 3489 Fragments arranged by size: 3489 3151 1365 384 BsrBI CCG'CTC Cuts at: 1394 1823 6276 1394 Size: 429 4453 3507 Fragments arranged by size: 4453 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4185 6884 7058 65 Size: 1183 2937 2699 174 1396 Fragments arranged by size: 2937 2699 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5583 7033 7220 1700 Size: 110 3773 1450 187 2869 Fragments arranged by size: 3773 2869 1450 187 110 BsrGI T'GTAC_A Cuts at: 96 3988 96 Size: 3892 4497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8389 BssSI C'ACGA_G Cuts at: 6449 7461 6449 Size: 1012 7377 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8389 BstBI TT'CG_AA Cuts at: 2007 7193 2007 Size: 5186 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8389 BstYI r'GATC_y Cuts at: 1972 4459 5338 5719 6086 6471 6488 7940 8026 Size: 2487 879 381 367 385 17 1452 86 Cuts at: 8026 8038 8384 1972 Size: 12 346 1977 Fragments arranged by size: 2487 1977 1452 879 385 381 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 6880 Fragments arranged by size: 6880 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7650 Fragments arranged by size: 7650 683 56 BtsI GCAGTG_nn' Cuts at: 2268 3706 5905 6699 6719 2268 Size: 1438 2199 794 20 3938 Fragments arranged by size: 3938 2199 1438 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8389 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8354 DraI TTT'AAA Cuts at: 4350 5693 4350 Size: 1343 7046 DraIII CAC_nnn'GTG Cuts at: 4580 4580 Size: 8389 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4237 7396 809 Size: 507 2921 3159 1802 Fragments arranged by size: 3159 2921 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3549 5836 6725 Size: 54 1425 50 275 18 1719 2287 889 Cuts at: 6725 8354 8 Size: 1629 43 Fragments arranged by size: 2287 1719 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5836 1537 Size: 275 4024 4090 Fragments arranged by size: 4090 4024 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2280 2787 2904 3092 3099 3150 3341 3468 Size: 927 507 117 188 7 51 191 127 Cuts at: 3468 3612 3633 3663 3672 4578 5627 6319 1353 Size: 144 21 30 9 906 1049 692 3423 Fragments arranged by size: 3423 1049 927 906 692 507 191 188 144 127 117 51 30 21 9 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6976 7350 7496 387 Size: 1064 138 5387 374 146 1280 Fragments arranged by size: 5387 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3786 3786 Size: 8389 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2910 2928 3636 4716 4856 5070 5681 Size: 393 1278 18 708 1080 140 214 611 Cuts at: 5681 6452 7049 7836 8104 1239 Size: 771 597 787 268 1524 Fragments arranged by size: 1524 1278 1080 787 771 708 611 597 393 268 214 140 18 EcoO109I rG'GnC_Cy Cuts at: 2648 3026 4706 2648 Size: 378 1680 6331 Fragments arranged by size: 6331 1680 378 EcoRI G'AATT_C Cuts at: 2291 5698 6076 2291 Size: 3407 378 4604 Fragments arranged by size: 4604 3407 378 EcoRV GAT'ATC Cuts at: 2194 2194 Size: 8389 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 3257 3305 2194 Size: 1063 48 7278 Fragments arranged by size: 7278 1063 48 FspI TGC'GCA Cuts at: 6895 6895 Size: 8389 HaeII r_GCGC'y Cuts at: 1678 7536 1678 Size: 5858 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 2669 2701 3469 Size: 32 135 32 800 32 265 32 768 Cuts at: 3469 3501 4668 4700 4953 4985 7030 7062 7104 Size: 32 1167 32 253 32 2045 32 42 Cuts at: 7104 7136 1373 Size: 32 2626 Fragments arranged by size: 2626 2045 1167 800 768 265 253 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4545 5422 5733 5979 669 Size: 988 281 2607 877 311 246 3079 Fragments arranged by size: 3079 2607 988 877 311 281 246 HindIII A'AGCT_T Cuts at: 748 3071 748 Size: 2323 6066 HpaI GTT'AAC Cuts at: 4545 5979 4545 Size: 1434 6955 KpnI G_GTAC'C Cuts at: 4487 5714 4487 Size: 1227 7162 MfeI C'AATT_G Cuts at: 4233 5988 4233 Size: 1755 6634 MluI A'CGCG_T Cuts at: 8265 8265 Size: 8389 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5076 7503 7687 1305 Size: 3771 2427 184 2007 Fragments arranged by size: 3771 2427 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3551 10 Size: 54 1425 343 1719 4848 Fragments arranged by size: 4848 1719 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3984 4472 4503 5395 6347 6706 6865 Size: 648 2818 488 31 892 952 359 159 Cuts at: 6865 518 Size: 2042 Fragments arranged by size: 2818 2042 952 892 648 488 359 159 31 MspA1I CmG'CkG Cuts at: 1606 2933 5805 6482 7630 7875 1606 Size: 1327 2872 677 1148 245 2120 Fragments arranged by size: 2872 2120 1327 1148 677 245 NaeI GCC'GGC Cuts at: 5585 5585 Size: 8389 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6880 Fragments arranged by size: 6880 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8389 NgoMIV G'CCGG_C Cuts at: 5583 5583 Size: 8389 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8389 NotI GC'GGCC_GC Cuts at: 5836 5836 Size: 8389 NsiI A_TGCA'T Cuts at: 4215 4215 Size: 8389 NspI r_CATG'y Cuts at: 1132 1229 3941 3989 5747 7292 1132 Size: 97 2712 48 1758 1545 2229 Fragments arranged by size: 2712 2229 1758 1545 97 48 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8389 PciI A'CATG_T Cuts at: 3985 7288 3985 Size: 3303 5086 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8389 PfoI T'CCnGG_A Cuts at: 4108 4693 4108 Size: 585 7804 PmeI GTTT'AAAC Cuts at: 5693 5693 Size: 8389 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6458 6490 7971 8003 6458 Size: 32 1481 32 6844 Fragments arranged by size: 6844 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2648 3026 2648 Size: 378 8011 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8389 PsiI TTA'TAA Cuts at: 4875 5959 4875 Size: 1084 7305 PspOMI G'GGCC_C Cuts at: 4706 4706 Size: 8389 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3444 3476 3444 Size: 32 8357 PstI C_TGCA'G Cuts at: 830 2095 2938 5741 830 Size: 1265 843 2803 3478 Fragments arranged by size: 3478 2803 1265 843 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8389 PvuII CAG'CTG Cuts at: 2933 2933 Size: 8389 SacI G_AGCT'C Cuts at: 3788 3788 Size: 8389 SalI G'TCGA_C Cuts at: 1936 5731 1936 Size: 3795 4594 SapI GCTCTTCn'nnn_ Cuts at: 4578 4578 Size: 8389 SbfI CC_TGCA'GG Cuts at: 5741 5741 Size: 8389 ScaI AGT'ACT Cuts at: 6637 8349 6637 Size: 1712 6677 SfcI C'TryA_G Cuts at: 826 1045 2091 2934 3438 4638 5556 5737 6872 Size: 219 1046 843 504 1200 918 181 1135 Cuts at: 6872 7553 7744 826 Size: 681 191 1471 Fragments arranged by size: 1471 1200 1135 1046 918 843 681 504 219 191 181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8389 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8389 SmaI CCC'GGG Cuts at: 1872 4623 5716 1872 Size: 2751 1093 4545 Fragments arranged by size: 4545 2751 1093 SmlI C'TyrA_G Cuts at: 820 1017 1943 2229 5334 6493 7394 7656 820 Size: 197 926 286 3105 1159 901 262 1553 Fragments arranged by size: 3105 1553 1159 926 901 286 262 197 SnaBI TAC'GTA Cuts at: 493 5686 6180 493 Size: 5193 494 2702 Fragments arranged by size: 5193 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8389 SphI G_CATG'C Cuts at: 1132 5747 1132 Size: 4615 3774 SspI AAT'ATT Cuts at: 5 52 6313 5 Size: 47 6261 2081 Fragments arranged by size: 6261 2081 47 StuI AGG'CCT Cuts at: 1517 4731 1517 Size: 3214 5175 StyI C'CwwG_G Cuts at: 513 1065 2022 2061 2085 5828 513 Size: 552 957 39 24 3743 3074 Fragments arranged by size: 3743 3074 957 552 39 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5407 5542 6416 6433 6586 6771 7216 Size: 1255 3827 135 874 17 153 185 445 Cuts at: 7216 325 Size: 1498 Fragments arranged by size: 3827 1498 1255 874 445 185 153 135 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 3988 5319 Size: 274 80 33 51 1443 444 1567 1331 Cuts at: 5319 6635 8347 96 Size: 1316 1712 138 Fragments arranged by size: 1712 1567 1443 1331 1316 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4024 4056 8349 8381 4024 Size: 32 4293 32 4032 Fragments arranged by size: 4293 4032 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8389 XbaI T'CTAG_A Cuts at: 2609 3964 5725 2609 Size: 1355 1761 5273 Fragments arranged by size: 5273 1761 1355 XhoI C'TCGA_G Cuts at: 1943 5334 1943 Size: 3391 4998 XmaI C'CCGG_G Cuts at: 1870 4621 5714 1870 Size: 2751 1093 4545 Fragments arranged by size: 4545 2751 1093 XmnI GAAnn'nnTTC Cuts at: 2563 6518 2563 Size: 3955 4434 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6197 276 Size: 53 83 186 936 4663 2468 Fragments arranged by size: 4663 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI NruI PacI PmlI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI