Restriction map for
pFN21ASDA0741
(Linear) (Six-base) MAPSORT of: KIAA0741.seq Check: 5885 from: 1 to: 3660
KIAA0741.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1972 3660
Size: 1972 1688
Acc65I G'GTAC_C
Cuts at: 0 2460 3660
Size: 2460 1200
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1613 3047 3658 3660
Size: 905 708 1434 611 2
Fragments arranged by size:
1434 905 708 611 2
AflIII A'CryG_T
Cuts at: 0 1962 3660
Size: 1962 1698
AleI CACnn'nnGTG
Cuts at: 0 2449 3372 3660
Size: 2449 923 288
Fragments arranged by size:
2449 923 288
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1550 1582 3660
Size: 1550 32 2078
Fragments arranged by size:
2078 1550 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1771 3660
Size: 1771 1889
ApaI G_GGCC'C
Cuts at: 0 2687 3660
Size: 2687 973
ApoI r'AATT_y
Cuts at: 0 268 540 574 823 1937 2058 2356 2389
Size: 268 272 34 249 1114 121 298 33
Cuts at: 2389 2986 3164 3247 3390 3660
Size: 597 178 83 143 270
Fragments arranged by size:
1114 597 298 272 270 268 249 178
143 121 83 34 33
AseI AT'TA_AT
Cuts at: 0 2033 3660
Size: 2033 1627
AvaI C'yCGr_G
Cuts at: 0 2598 3311 3660
Size: 2598 713 349
Fragments arranged by size:
2598 713 349
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 854 887 3660
Size: 854 33 2773
Fragments arranged by size:
2773 854 33
BanI G'GyrC_C
Cuts at: 0 2460 2608 3660
Size: 2460 148 1052
Fragments arranged by size:
2460 1052 148
BanII G_rGCy'C
Cuts at: 0 1765 1893 2204 2687 3660
Size: 1765 128 311 483 973
Fragments arranged by size:
1765 973 483 311 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 35 1735 1936 3525 3660
Size: 35 1700 201 1589 135
Fragments arranged by size:
1700 1589 201 135 35
BclI T'GATC_A
Cuts at: 0 2630 3660
Size: 2630 1030
BfrBI ATG'CAT
Cuts at: 0 2190 3660
Size: 2190 1470
BlpI GC'TnA_GC
Cuts at: 0 1046 3660
Size: 1046 2614
Bme1580I G_kGCm'C
Cuts at: 0 2611 2687 3011 3660
Size: 2611 76 324 649
Fragments arranged by size:
2611 649 324 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 2971 3028 3660
Size: 2971 57 632
Fragments arranged by size:
2971 632 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 2693 2833 3660
Size: 887 1806 140 827
Fragments arranged by size:
1806 887 827 140
Bpu10I CC'TnA_GC
Cuts at: 0 1283 2783 3660
Size: 1283 1500 877
Fragments arranged by size:
1500 1283 877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 3660
Size: 191 3469
BsaBI GATnn'nnATC
Cuts at: 0 1790 3283 3660
Size: 1790 1493 377
Fragments arranged by size:
1790 1493 377
BsaHI Gr'CG_yC
Cuts at: 0 659 3660
Size: 659 3001
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1446 1476 2930 2960 3660
Size: 1446 30 1454 30 700
Fragments arranged by size:
1454 1446 700 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 1552 1612 1615 1618 1621 1663 1672
Size: 508 1044 60 3 3 3 42 9
Cuts at: 1672 2377 2711 3660
Size: 705 334 949
Fragments arranged by size:
1044 949 705 508 334 60 42 9
3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2456 3194 3660
Size: 2456 738 466
Fragments arranged by size:
2456 738 466
BsiHKAI G_wGCw'C
Cuts at: 0 1765 2574 3660
Size: 1765 809 1086
Fragments arranged by size:
1765 1086 809
BsmI GAATG_Cn'
Cuts at: 0 2096 2415 3660
Size: 2096 319 1245
Fragments arranged by size:
2096 1245 319
Bsp1286I G_dGCh'C
Cuts at: 0 1765 1893 2204 2574 2611 2687 3011 3660
Size: 1765 128 311 370 37 76 324 649
Fragments arranged by size:
1765 649 370 324 311 128 76 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1972 3337 3660
Size: 1972 1365 323
Fragments arranged by size:
1972 1365 323
BsrDI GCAATG_nn'
Cuts at: 0 2162 3660
Size: 2162 1498
BsrFI r'CCGG_y
Cuts at: 0 3560 3660
Size: 3560 100
BsrGI T'GTAC_A
Cuts at: 0 1965 3660
Size: 1965 1695
BstYI r'GATC_y
Cuts at: 0 2436 3315 3660
Size: 2436 879 345
Fragments arranged by size:
2436 879 345
BtsI GCAGTG_nn'
Cuts at: 0 245 1683 3660
Size: 245 1438 1977
Fragments arranged by size:
1977 1438 245
DraI TTT'AAA
Cuts at: 0 2327 3660
Size: 2327 1333
DraIII CAC_nnn'GTG
Cuts at: 0 2557 3660
Size: 2557 1103
DrdI GACnn_nn'nnGTC
Cuts at: 0 2214 3660
Size: 2214 1446
EaeI y'GGCC_r
Cuts at: 0 1526 3660
Size: 1526 2134
EarI CTCTTCn'nnn_
Cuts at: 0 257 764 881 1069 1076 1127 1318 1445
Size: 257 507 117 188 7 51 191 127
Cuts at: 1445 1589 1610 1640 1649 2555 3604 3660
Size: 144 21 30 9 906 1049 56
Fragments arranged by size:
1049 906 507 257 191 188 144 127
117 56 51 30 21 9 7
EcoICRI GAG'CTC
Cuts at: 0 1763 3660
Size: 1763 1897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 905 1613 2693 2833 3047 3658 3660
Size: 887 18 708 1080 140 214 611 2
Fragments arranged by size:
1080 887 708 611 214 140 18 2
EcoO109I rG'GnC_Cy
Cuts at: 0 625 1003 2683 3660
Size: 625 378 1680 977
Fragments arranged by size:
1680 977 625 378
EcoRI G'AATT_C
Cuts at: 0 268 3660
Size: 268 3392
EcoRV GAT'ATC
Cuts at: 0 171 3660
Size: 171 3489
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 171 1234 1282 3660
Size: 171 1063 48 2378
Fragments arranged by size:
2378 1063 171 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 646 678 1446 1478 2645 2677
Size: 349 32 265 32 768 32 1167 32
Cuts at: 2677 2930 2962 3660
Size: 253 32 698
Fragments arranged by size:
1167 768 698 349 265 253 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2522 3399 3660
Size: 2522 877 261
Fragments arranged by size:
2522 877 261
HindIII A'AGCT_T
Cuts at: 0 1048 3660
Size: 1048 2612
HpaI GTT'AAC
Cuts at: 0 2522 3660
Size: 2522 1138
KpnI G_GTAC'C
Cuts at: 0 2464 3660
Size: 2464 1196
MfeI C'AATT_G
Cuts at: 0 2210 3660
Size: 2210 1450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3053 3660
Size: 3053 607
MscI TGG'CCA
Cuts at: 0 1528 3660
Size: 1528 2132
MslI CAynn'nnrTG
Cuts at: 0 1961 2449 2480 3372 3660
Size: 1961 488 31 892 288
Fragments arranged by size:
1961 892 488 288 31
MspA1I CmG'CkG
Cuts at: 0 910 3660
Size: 910 2750
NaeI GCC'GGC
Cuts at: 0 3562 3660
Size: 3562 98
NgoMIV G'CCGG_C
Cuts at: 0 3560 3660
Size: 3560 100
NsiI A_TGCA'T
Cuts at: 0 2192 3660
Size: 2192 1468
NspI r_CATG'y
Cuts at: 0 1918 1966 3660
Size: 1918 48 1694
Fragments arranged by size:
1918 1694 48
PciI A'CATG_T
Cuts at: 0 1962 3660
Size: 1962 1698
PfoI T'CCnGG_A
Cuts at: 0 2085 2670 3660
Size: 2085 585 990
Fragments arranged by size:
2085 990 585
PpuMI rG'GwC_Cy
Cuts at: 0 625 1003 3660
Size: 625 378 2657
Fragments arranged by size:
2657 625 378
PsiI TTA'TAA
Cuts at: 0 2852 3660
Size: 2852 808
PspOMI G'GGCC_C
Cuts at: 0 2683 3660
Size: 2683 977
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1421 1453 3660
Size: 1421 32 2207
Fragments arranged by size:
2207 1421 32
PstI C_TGCA'G
Cuts at: 0 72 915 3660
Size: 72 843 2745
Fragments arranged by size:
2745 843 72
PvuII CAG'CTG
Cuts at: 0 910 3660
Size: 910 2750
SacI G_AGCT'C
Cuts at: 0 1765 3660
Size: 1765 1895
SapI GCTCTTCn'nnn_
Cuts at: 0 2555 3660
Size: 2555 1105
SfcI C'TryA_G
Cuts at: 0 68 911 1415 2615 3533 3660
Size: 68 843 504 1200 918 127
Fragments arranged by size:
1200 918 843 504 127 68
SmaI CCC'GGG
Cuts at: 0 2600 3660
Size: 2600 1060
SmlI C'TyrA_G
Cuts at: 0 206 3311 3660
Size: 206 3105 349
Fragments arranged by size:
3105 349 206
StuI AGG'CCT
Cuts at: 0 2708 3660
Size: 2708 952
StyI C'CwwG_G
Cuts at: 0 38 62 3660
Size: 38 24 3598
Fragments arranged by size:
3598 38 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3384 3519 3660
Size: 3384 135 141
Fragments arranged by size:
3384 141 135
TatI w'GTAC_w
Cuts at: 0 398 1965 3296 3660
Size: 398 1567 1331 364
Fragments arranged by size:
1567 1331 398 364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2001 2033 3660
Size: 2001 32 1627
Fragments arranged by size:
2001 1627 32
XbaI T'CTAG_A
Cuts at: 0 586 1941 3660
Size: 586 1355 1719
Fragments arranged by size:
1719 1355 586
XhoI C'TCGA_G
Cuts at: 0 3311 3660
Size: 3311 349
XmaI C'CCGG_G
Cuts at: 0 2598 3660
Size: 2598 1062
XmnI GAAnn'nnTTC
Cuts at: 0 540 3660
Size: 540 3120
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AloI AlwNI ApaI
ApoI AseI AvaI BaeI BanI BanII BbsI BclI
BfrBI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI
BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstYI BtsI DraI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI
MslI MspA1I NaeI NgoMIV NsiI NspI PciI PfoI
PpuMI PsiI PspOMI PsrI PstI PvuII SacI SapI
SfcI SmaI SmlI StuI StyI TaqII TatI TstI
XbaI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlfI
ApaLI AscI AsiSI AvrII BamHI BbeI BbvCI BcgI
BciVI BglI BglII BmgBI BmtI BplI BsaI BsaAI
BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI EagI EciI EcoNI FseI
FspI FspAI HaeII KasI MluI NarI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PmeI PmlI
PpiI PshAI PspXI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XcmI ZraI