Restriction map for
pFN21ASDA0680
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BanI BanII BbeI BbrPI BcnI
BglI BglII BlnI BsaAI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI BspDI BspEI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
KpnI KspI MaeII MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 1874 GTCGAC GT^[AC][GT]AC
AciI 1767 CCGC CCGC(-3/-1)
BamHI 1715 GGATCC G^GATCC
BcgI 700 GCATCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1428 GGATAC GTATCC(6/5)
BclI 255 TGATCA T^GATCA
BmyI 298 GGGCAC G[AGT]GC[ACT]^C
BsaBI 306 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1092 CGTCTC CGTCTC(1/5)
Bsp1286I 298 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 1349 TGTACA T^GTACA
BspHI 1863 TCATGA T^CATGA
BsrFI 447 ACCGGC [GA]^CCGG[CT]
BsrGI 1349 TGTACA T^GTACA
BstAPI 451 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 447 ACCGGC [GA]^CCGG[CT]
DraI 1840 TTTAAA TTT^AAA
DraII 608 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 1084 GACCTGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 965 CCATGG C^C[GA][CT]GG
EcoO109I 608 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 195 GATATC GAT^ATC
FbaI 255 TGATCA T^GATCA
MamI 306 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 965 CCATGG C^CATGG
PshAI 1105 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1863 TCATGA T^CATGA
SalI 1874 GTCGAC G^TCGAC
SapI 1129 GAAGAGC GCTCTTC(1/4)
SfaNI 700 GCATC GCATC(5/9)
SspBI 1349 TGTACA T^GTACA
Tsp45I 1744 GTCAC ^GT[GC]AC
XcmI 1208 CCAAAGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 1350 GTAC GT^AC
1499 GTAC
AflII 241 CTTAAG C^TTAAG
1170 CTTAAG
AvaI 329 CCCGAG C^[CT]CG[GA]G
650 CCCGAG
AvaII 785 GGACC G^G[AT]CC
969 GGTCC
BbvCI 1234 CCTCAGC CCTCAGC(-5/-2)
934 GCTGAGG
BfrI 241 CTTAAG C^TTAAG
1170 CTTAAG
BlpI 1560 GCTGAGC GC^T[ACGT]AGC
1674 GCTCAGC
Bpu1102I 1560 GCTGAGC GC^T[ACGT]AGC
1674 GCTCAGC
BsaI 889 GAGACC GGTCTC(1/5)
1683 GAGACC
BsoBI 329 CCCGAG C^[CT]CG[GA]G
650 CCCGAG
BstYI 490 AGATCC [GA]^GATC[CT]
1715 GGATCC
CelII 1560 GCTGAGC GC^T[ACGT]AGC
1674 GCTCAGC
EcoNI 523 CCTCCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1784 CCTGGGCCAGG
HapII 448 CCGG C^CGG
1422 CCGG
HindIII 29 AAGCTT A^AGCTT
743 AAGCTT
HpaII 448 CCGG C^CGG
1422 CCGG
MaeIII 271 GTAAC ^GT[ACGT]AC
1744 GTCAC
MflI 490 AGATCC [GA]^GATC[CT]
1715 GGATCC
MslI 553 CACAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1848 CACAGAAATG
MspI 448 CCGG C^CGG
1422 CCGG
PstI 884 CTGCAG CTGCA^G
1802 CTGCAG
RsaI 1350 GTAC GT^AC
1499 GTAC
XhoII 490 AGATCC [GA]^GATC[CT]
1715 GGATCC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AluI AlwI AlwNI ApoI
BbsI BbvI BfaI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HpaI
HphI ItaI Ksp632I MaeI MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I