(Circular) (Six-base) MAPSORT of: pFN21ASDA0652.seq Check: 957 from: 1 to: 6280 pFN21ASDA0652 6280 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4090 278 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2612 3623 1937 Size: 675 1011 4594 Fragments arranged by size: 4594 1011 675 Acc65I G'GTAC_C Cuts at: 2950 2981 3601 2950 Size: 31 620 5629 Fragments arranged by size: 5629 620 31 AclI AA'CG_TT Cuts at: 1563 4407 4780 1563 Size: 2844 373 3063 Fragments arranged by size: 3063 2844 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2417 3510 4343 5727 5995 1632 Size: 785 1093 833 1384 268 1917 Fragments arranged by size: 1917 1384 1093 833 785 268 AflII C'TTAA_G Cuts at: 820 1017 2279 820 Size: 197 1262 4821 Fragments arranged by size: 4821 1262 197 AflIII A'CryG_T Cuts at: 1203 5179 6156 1203 Size: 3976 977 1327 Fragments arranged by size: 3976 1327 977 AgeI A'CCGG_T Cuts at: 5111 5111 Size: 6280 AhdI GACnn_n'nnGTC Cuts at: 5009 5009 Size: 6280 AleI CACnn'nnGTG Cuts at: 1166 2558 1166 Size: 1392 4888 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6280 AlwNI CAG_nnn'CTG Cuts at: 3278 4023 5595 3278 Size: 745 1572 3963 Fragments arranged by size: 3963 1572 745 ApaLI G'TGCA_C Cuts at: 4337 5493 4337 Size: 1156 5124 ApoI r'AATT_y Cuts at: 1493 2075 3223 3589 3815 3967 6076 1493 Size: 582 1148 366 226 152 2109 1697 Fragments arranged by size: 2109 1697 1148 582 366 226 152 AseI AT'TA_AT Cuts at: 160 1032 4834 160 Size: 872 3802 1606 Fragments arranged by size: 3802 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 AvaI C'yCGr_G Cuts at: 1870 1943 3605 1870 Size: 73 1662 4545 Fragments arranged by size: 4545 1662 73 BamHI G'GATC_C Cuts at: 3610 3977 3610 Size: 367 5913 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2552 2564 2950 2981 3601 Size: 325 217 627 765 12 386 31 620 Cuts at: 3601 5056 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 765 627 620 386 325 217 31 12 BanII G_rGCy'C Cuts at: 1391 3599 1391 Size: 2208 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2766 3498 928 Size: 971 867 732 3710 Fragments arranged by size: 3710 971 867 732 BbvCI CC'TCA_GC Cuts at: 3444 3444 Size: 6280 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3533 3567 4471 4505 1903 Size: 34 1596 34 904 34 3678 Fragments arranged by size: 3678 1596 904 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3058 3232 4172 5382 3058 Size: 174 940 1210 3956 Fragments arranged by size: 3956 1210 940 174 BclI T'GATC_A Cuts at: 1548 2659 1548 Size: 1111 5169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4891 136 Size: 107 122 71 631 605 3219 1525 Fragments arranged by size: 3219 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6275 6275 Size: 6280 BlpI GC'TnA_GC Cuts at: 3449 3697 3449 Size: 248 6032 Bme1580I G_kGCm'C Cuts at: 1790 2555 2567 4341 5497 1790 Size: 765 12 1774 1156 2573 Fragments arranged by size: 2573 1774 1156 765 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6280 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4011 4969 442 Size: 416 53 83 399 24 2594 958 1753 Fragments arranged by size: 2594 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2428 2757 3037 3246 3254 3275 3301 4940 Size: 1189 329 280 209 8 21 26 1639 Cuts at: 4940 1239 Size: 2579 Fragments arranged by size: 2579 1639 1189 329 280 209 26 21 8 Bpu10I CC'TnA_GC Cuts at: 2512 3444 2512 Size: 932 5348 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2072 2751 2800 4405 5270 5568 2072 Size: 679 49 1605 865 298 2784 Fragments arranged by size: 2784 1605 865 679 298 49 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3116 4943 882 Size: 617 1617 1827 2219 Fragments arranged by size: 2219 1827 1617 617 BsaAI yAC'GTr Cuts at: 493 1204 3577 4071 493 Size: 711 2373 494 2702 Fragments arranged by size: 2702 2373 711 494 BsaBI GATnn'nnATC Cuts at: 3243 6274 3243 Size: 3031 3249 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4087 4469 5104 275 Size: 53 83 186 936 2554 382 635 1451 Fragments arranged by size: 2554 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4713 5111 5385 5532 5943 4713 Size: 398 274 147 411 5050 Fragments arranged by size: 5050 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3268 3298 1178 Size: 30 165 30 1865 30 4160 Fragments arranged by size: 4160 1865 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2680 3276 1186 Size: 1494 596 4190 Fragments arranged by size: 4190 1494 596 BseYI C'CCAG_C Cuts at: 2684 2808 5483 5979 2684 Size: 124 2675 496 2985 Fragments arranged by size: 2985 2675 496 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2998 3287 1163 Size: 573 1262 289 4156 Fragments arranged by size: 4156 1262 573 289 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3730 4491 5519 1540 Size: 275 186 1729 761 1028 2301 Fragments arranged by size: 2301 1729 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3478 4341 4426 5497 3478 Size: 863 85 1071 4261 Fragments arranged by size: 4261 1071 863 85 BsmI GAATG_Cn' Cuts at: 2603 3791 3884 2603 Size: 1188 93 4999 Fragments arranged by size: 4999 1188 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2555 2567 3478 3599 4341 4426 5497 Size: 399 765 12 911 121 742 85 1071 Cuts at: 5497 1391 Size: 2174 Fragments arranged by size: 2174 1071 911 765 742 399 121 85 12 BspEI T'CCGG_A Cuts at: 5943 5943 Size: 6280 BspHI T'CATG_A Cuts at: 4169 5899 4169 Size: 1730 4550 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3020 3635 844 Size: 2176 615 3489 Fragments arranged by size: 3489 2176 615 BsrBI CCG'CTC Cuts at: 1394 1823 4167 1394 Size: 429 2344 3507 Fragments arranged by size: 3507 2344 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2387 4775 4949 65 Size: 1183 1139 2388 174 1396 Fragments arranged by size: 2388 1396 1183 1139 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4924 5111 1700 Size: 110 3114 187 2869 Fragments arranged by size: 3114 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2373 96 Size: 2277 4003 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6280 BssSI C'ACGA_G Cuts at: 4340 5352 4340 Size: 1012 5268 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6280 BstBI TT'CG_AA Cuts at: 2007 5084 2007 Size: 3077 3203 BstEII G'GTnAC_C Cuts at: 1184 3175 1184 Size: 1991 4289 BstXI CCAn_nnnn'nTGG Cuts at: 2266 2308 3226 2266 Size: 42 918 5320 Fragments arranged by size: 5320 918 42 BstYI r'GATC_y Cuts at: 1972 3610 3977 4362 4379 5831 5917 5929 6275 Size: 1638 367 385 17 1452 86 12 346 Cuts at: 6275 1972 Size: 1977 Fragments arranged by size: 1977 1638 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2301 2977 3483 513 Size: 552 957 279 676 506 3310 Fragments arranged by size: 3310 957 676 552 506 279 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5541 Fragments arranged by size: 5541 683 56 BtsI GCAGTG_nn' Cuts at: 3094 3337 3796 4590 4610 3094 Size: 243 459 794 20 4764 Fragments arranged by size: 4764 794 459 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6280 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6245 DraI TTT'AAA Cuts at: 3370 3584 3370 Size: 214 6066 DraIII CAC_nnn'GTG Cuts at: 2668 2668 Size: 6280 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5287 809 Size: 507 3971 1802 Fragments arranged by size: 3971 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3727 4616 6245 Size: 54 1425 50 275 18 1897 889 1629 Cuts at: 6245 8 Size: 43 Fragments arranged by size: 1897 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3727 1537 Size: 275 1915 4090 Fragments arranged by size: 4090 1915 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4210 1353 Size: 2857 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4867 5241 5387 387 Size: 1064 138 3278 374 146 1280 Fragments arranged by size: 3278 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2428 2757 3037 3246 3254 3275 Size: 393 785 11 329 280 209 8 21 Cuts at: 3275 3301 3510 4343 4940 5727 5995 1239 Size: 26 209 833 597 787 268 1524 Fragments arranged by size: 1524 833 787 785 597 393 329 280 268 209 209 26 21 11 8 EcoO109I rG'GnC_Cy Cuts at: 2056 2631 3414 2056 Size: 575 783 4922 Fragments arranged by size: 4922 783 575 EcoRI G'AATT_C Cuts at: 3589 3967 3589 Size: 378 5902 FspI TGC'GCA Cuts at: 4786 4786 Size: 6280 HaeII r_GCGC'y Cuts at: 1678 5427 1678 Size: 3749 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2122 2154 3266 3268 3298 Size: 32 135 32 550 32 1112 2 30 Cuts at: 3298 3300 3406 3438 3485 3517 4921 4953 4995 Size: 2 106 32 47 32 1404 32 42 Cuts at: 4995 5027 1373 Size: 32 2626 Fragments arranged by size: 2626 1404 1112 550 135 106 47 42 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3157 3624 3870 669 Size: 988 281 1219 467 246 3079 Fragments arranged by size: 3079 1219 988 467 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6280 HpaI GTT'AAC Cuts at: 3870 3870 Size: 6280 KpnI G_GTAC'C Cuts at: 2954 2985 3605 2954 Size: 31 620 5629 Fragments arranged by size: 5629 620 31 MfeI C'AATT_G Cuts at: 3879 3879 Size: 6280 MluI A'CGCG_T Cuts at: 6156 6156 Size: 6280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2180 2836 5394 5578 1305 Size: 875 656 2558 184 2007 Fragments arranged by size: 2558 2007 875 656 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4458 Fragments arranged by size: 4458 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2264 2463 2558 3309 4238 4597 4756 Size: 648 1098 199 95 751 929 359 159 Cuts at: 4756 518 Size: 2042 Fragments arranged by size: 2042 1098 929 751 648 359 199 159 95 MspA1I CmG'CkG Cuts at: 1606 2239 2503 2864 2923 3321 3448 3696 4373 Size: 633 264 361 59 398 127 248 677 Cuts at: 4373 5521 5766 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 633 398 361 264 248 245 127 59 NcoI C'CATG_G Cuts at: 513 1065 2022 2301 2977 3483 513 Size: 552 957 279 676 506 3310 Fragments arranged by size: 3310 957 676 552 506 279 NdeI CA'TA_TG Cuts at: 387 387 Size: 6280 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6280 NotI GC'GGCC_GC Cuts at: 3727 3727 Size: 6280 NspI r_CATG'y Cuts at: 1132 1229 3638 5183 1132 Size: 97 2409 1545 2229 Fragments arranged by size: 2409 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2848 1348 Size: 1500 4780 PciI A'CATG_T Cuts at: 5179 5179 Size: 6280 PflMI CCAn_nnn'nTGG Cuts at: 1108 2926 1108 Size: 1818 4462 PfoI T'CCnGG_A Cuts at: 2444 2444 Size: 6280 PmeI GTTT'AAAC Cuts at: 3584 3584 Size: 6280 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4349 4381 5862 5894 4349 Size: 32 1481 32 4735 Fragments arranged by size: 4735 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2631 3414 2056 Size: 575 783 4922 Fragments arranged by size: 4922 783 575 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6280 PsiI TTA'TAA Cuts at: 3850 3850 Size: 6280 PstI C_TGCA'G Cuts at: 830 2248 3573 3632 830 Size: 1418 1325 59 3478 Fragments arranged by size: 3478 1418 1325 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6280 PvuII CAG'CTG Cuts at: 2239 2503 2864 2923 3448 2239 Size: 264 361 59 525 5071 Fragments arranged by size: 5071 525 361 264 59 SalI G'TCGA_C Cuts at: 1936 3622 1936 Size: 1686 4594 SbfI CC_TGCA'GG Cuts at: 3632 3632 Size: 6280 ScaI AGT'ACT Cuts at: 4528 6240 4528 Size: 1712 4568 SexAI A'CCwGG_T Cuts at: 3171 3171 Size: 6280 SfcI C'TryA_G Cuts at: 826 1045 2244 2434 2754 3569 3628 4763 5444 Size: 219 1199 190 320 815 59 1135 681 Cuts at: 5444 5635 826 Size: 191 1471 Fragments arranged by size: 1471 1199 1135 815 681 320 219 191 190 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6280 SmaI CCC'GGG Cuts at: 1872 3607 1872 Size: 1735 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2087 2279 2766 2815 4384 5285 Size: 197 926 144 192 487 49 1569 901 Cuts at: 5285 5547 820 Size: 262 1553 Fragments arranged by size: 1569 1553 926 901 487 262 197 192 144 49 SnaBI TAC'GTA Cuts at: 493 3577 4071 493 Size: 3084 494 2702 Fragments arranged by size: 3084 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6280 SphI G_CATG'C Cuts at: 1132 3638 1132 Size: 2506 3774 SspI AAT'ATT Cuts at: 5 52 4204 5 Size: 47 4152 2081 Fragments arranged by size: 4152 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6280 StyI C'CwwG_G Cuts at: 513 1065 2022 2301 2977 3483 3719 513 Size: 552 957 279 676 506 236 3074 Fragments arranged by size: 3074 957 676 552 506 279 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4307 4324 4477 4662 5107 325 Size: 1255 2727 17 153 185 445 1498 Fragments arranged by size: 2727 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2373 4526 6238 Size: 274 80 33 51 1443 396 2153 1712 Cuts at: 6238 96 Size: 138 Fragments arranged by size: 2153 1712 1443 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2303 2335 2929 2961 6240 6272 2303 Size: 32 594 32 3279 32 2311 Fragments arranged by size: 3279 2311 594 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6280 XbaI T'CTAG_A Cuts at: 3616 3616 Size: 6280 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2117 2975 2117 Size: 858 5422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6280 XmaI C'CCGG_G Cuts at: 1870 3605 1870 Size: 1735 4545 XmnI GAAnn'nnTTC Cuts at: 4409 4409 Size: 6280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4088 276 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI