Restriction map for
pFN21ASDA0652
(Linear) (Six-base) MAPSORT of: KIAA0652.seq Check: 7095 from: 1 to: 1551
KIAA0652.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 589 1551
Size: 589 962
Acc65I G'GTAC_C
Cuts at: 0 927 958 1551
Size: 927 31 593
Fragments arranged by size:
927 593 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1487 1551
Size: 394 1093 64
Fragments arranged by size:
1093 394 64
AflII C'TTAA_G
Cuts at: 0 256 1551
Size: 256 1295
AleI CACnn'nnGTG
Cuts at: 0 535 1551
Size: 535 1016
AlwNI CAG_nnn'CTG
Cuts at: 0 1255 1551
Size: 1255 296
ApoI r'AATT_y
Cuts at: 0 52 1200 1551
Size: 52 1148 351
Fragments arranged by size:
1148 351 52
BanI G'GyrC_C
Cuts at: 0 529 541 927 958 1551
Size: 529 12 386 31 593
Fragments arranged by size:
593 529 386 31 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 743 1475 1551
Size: 743 732 76
Fragments arranged by size:
743 732 76
BbvCI CC'TCA_GC
Cuts at: 0 1421 1551
Size: 1421 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1510 1544 1551
Size: 1510 34 7
Fragments arranged by size:
1510 34 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 1035 1209 1551
Size: 1035 174 342
Fragments arranged by size:
1035 342 174
BclI T'GATC_A
Cuts at: 0 636 1551
Size: 636 915
BlpI GC'TnA_GC
Cuts at: 0 1426 1551
Size: 1426 125
Bme1580I G_kGCm'C
Cuts at: 0 532 544 1551
Size: 532 12 1007
Fragments arranged by size:
1007 532 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 734 1014 1223 1231 1252 1278 1551
Size: 405 329 280 209 8 21 26 273
Fragments arranged by size:
405 329 280 273 209 26 21 8
Bpu10I CC'TnA_GC
Cuts at: 0 489 1421 1551
Size: 489 932 130
Fragments arranged by size:
932 489 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 728 777 1551
Size: 49 679 49 774
Fragments arranged by size:
774 679 49 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 1093 1551
Size: 1093 458
BsaBI GATnn'nnATC
Cuts at: 0 1220 1551
Size: 1220 331
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1245 1275 1551
Size: 1245 30 276
Fragments arranged by size:
1245 276 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 657 1253 1551
Size: 657 596 298
Fragments arranged by size:
657 596 298
BseYI C'CCAG_C
Cuts at: 0 661 785 1551
Size: 661 124 766
Fragments arranged by size:
766 661 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 975 1264 1551
Size: 975 289 287
Fragments arranged by size:
975 289 287
BsiHKAI G_wGCw'C
Cuts at: 0 1455 1551
Size: 1455 96
BsmI GAATG_Cn'
Cuts at: 0 580 1551
Size: 580 971
Bsp1286I G_dGCh'C
Cuts at: 0 532 544 1455 1551
Size: 532 12 911 96
Fragments arranged by size:
911 532 96 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 997 1551
Size: 997 554
BsrDI GCAATG_nn'
Cuts at: 0 364 1551
Size: 364 1187
BsrGI T'GTAC_A
Cuts at: 0 350 1551
Size: 350 1201
BstEII G'GTnAC_C
Cuts at: 0 1152 1551
Size: 1152 399
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 285 1203 1551
Size: 243 42 918 348
Fragments arranged by size:
918 348 243 42
BtgI C'CryG_G
Cuts at: 0 278 954 1460 1551
Size: 278 676 506 91
Fragments arranged by size:
676 506 278 91
BtsI GCAGTG_nn'
Cuts at: 0 1071 1314 1551
Size: 1071 243 237
Fragments arranged by size:
1071 243 237
DraI TTT'AAA
Cuts at: 0 1347 1551
Size: 1347 204
DraIII CAC_nnn'GTG
Cuts at: 0 645 1551
Size: 645 906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 405 734 1014 1223 1231 1252 1278
Size: 394 11 329 280 209 8 21 26
Cuts at: 1278 1487 1551
Size: 209 64
Fragments arranged by size:
394 329 280 209 209 64 26 21
11 8
EcoO109I rG'GnC_Cy
Cuts at: 0 33 608 1391 1551
Size: 33 575 783 160
Fragments arranged by size:
783 575 160 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 99 131 1243 1245 1275 1277 1383 1415
Size: 99 32 1112 2 30 2 106 32
Cuts at: 1415 1462 1494 1551
Size: 47 32 57
Fragments arranged by size:
1112 106 99 57 47 32 32 32
30 2 2
HincII GTy'rAC
Cuts at: 0 1134 1551
Size: 1134 417
KpnI G_GTAC'C
Cuts at: 0 931 962 1551
Size: 931 31 589
Fragments arranged by size:
931 589 31
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 813 1551
Size: 157 656 738
Fragments arranged by size:
738 656 157
MslI CAynn'nnrTG
Cuts at: 0 241 440 535 1286 1551
Size: 241 199 95 751 265
Fragments arranged by size:
751 265 241 199 95
MspA1I CmG'CkG
Cuts at: 0 216 480 841 900 1298 1425 1551
Size: 216 264 361 59 398 127 126
Fragments arranged by size:
398 361 264 216 127 126 59
NcoI C'CATG_G
Cuts at: 0 278 954 1460 1551
Size: 278 676 506 91
Fragments arranged by size:
676 506 278 91
PasI CC'CwG_GG
Cuts at: 0 825 1551
Size: 825 726
PflMI CCAn_nnn'nTGG
Cuts at: 0 903 1551
Size: 903 648
PfoI T'CCnGG_A
Cuts at: 0 421 1551
Size: 421 1130
PpuMI rG'GwC_Cy
Cuts at: 0 33 608 1391 1551
Size: 33 575 783 160
Fragments arranged by size:
783 575 160 33
PstI C_TGCA'G
Cuts at: 0 225 1550 1551
Size: 225 1325 1
Fragments arranged by size:
1325 225 1
PvuII CAG'CTG
Cuts at: 0 216 480 841 900 1425 1551
Size: 216 264 361 59 525 126
Fragments arranged by size:
525 361 264 216 126 59
SexAI A'CCwGG_T
Cuts at: 0 1148 1551
Size: 1148 403
SfcI C'TryA_G
Cuts at: 0 221 411 731 1546 1551
Size: 221 190 320 815 5
Fragments arranged by size:
815 320 221 190 5
SmlI C'TyrA_G
Cuts at: 0 64 256 743 792 1551
Size: 64 192 487 49 759
Fragments arranged by size:
759 487 192 64 49
StyI C'CwwG_G
Cuts at: 0 278 954 1460 1551
Size: 278 676 506 91
Fragments arranged by size:
676 506 278 91
TatI w'GTAC_w
Cuts at: 0 350 1551
Size: 350 1201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 280 312 906 938 1551
Size: 280 32 594 32 613
Fragments arranged by size:
613 594 280 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 94 952 1551
Size: 94 858 599
Fragments arranged by size:
858 599 94
Enzymes that do cut:
AccI Acc65I AcuI AflII AleI AlwNI ApoI BanI
BbsI BbvCI BcgI BciVI BclI BlpI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstEII BstXI
BtgI BtsI DraI DraIII Eco57MI EcoO109I Hin4I HincII
KpnI MmeI MslI MspA1I NcoI PasI PflMI PfoI
PpuMI PstI PvuII SexAI SfcI SmlI StyI TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BfrBI BglI BglII BmgBI
BmrI BmtI BplI BsaAI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XhoI XmaI
XmnI ZraI