(Circular) (Six-base) MAPSORT of: pFN21ASDA0617.seq Check: 6209 from: 1 to: 7033 pFN21ASDA0617 7033 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4843 278 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2439 2556 3658 4107 4311 4376 1937 Size: 502 117 1102 449 204 65 4594 Fragments arranged by size: 4594 1102 502 449 204 117 65 Acc65I G'GTAC_C Cuts at: 4354 4354 Size: 7033 AclI AA'CG_TT Cuts at: 1563 3310 5160 5533 1563 Size: 1747 1850 373 3063 Fragments arranged by size: 3063 1850 1747 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2697 3033 3086 3762 5096 6480 6748 1632 Size: 1065 336 53 676 1334 1384 268 1917 Fragments arranged by size: 1917 1384 1334 1065 676 336 268 53 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6836 AflIII A'CryG_T Cuts at: 1203 5932 6909 1203 Size: 4729 977 1327 Fragments arranged by size: 4729 1327 977 AgeI A'CCGG_T Cuts at: 5864 5864 Size: 7033 AhdI GACnn_n'nnGTC Cuts at: 5762 5762 Size: 7033 AleI CACnn'nnGTG Cuts at: 1166 3583 1166 Size: 2417 4616 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7033 AlwNI CAG_nnn'CTG Cuts at: 4055 4776 6348 4055 Size: 721 1572 4740 Fragments arranged by size: 4740 1572 721 ApaLI G'TGCA_C Cuts at: 3759 5090 6246 3759 Size: 1331 1156 4546 Fragments arranged by size: 4546 1331 1156 ApoI r'AATT_y Cuts at: 1493 2305 2666 2690 2863 3479 3867 4342 4568 Size: 812 361 24 173 616 388 475 226 Cuts at: 4568 4720 6829 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 812 616 475 388 361 226 173 152 24 AseI AT'TA_AT Cuts at: 160 1032 2453 2745 3827 5587 160 Size: 872 1421 292 1082 1760 1606 Fragments arranged by size: 1760 1606 1421 1082 872 292 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 AvaI C'yCGr_G Cuts at: 1870 1943 4358 1870 Size: 73 2415 4545 Fragments arranged by size: 4545 2415 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3492 3525 3492 Size: 33 7000 BamHI G'GATC_C Cuts at: 2073 4363 4730 2073 Size: 2290 367 4376 Fragments arranged by size: 4376 2290 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4354 5809 618 Size: 325 217 627 2567 1455 1842 Fragments arranged by size: 2567 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2193 3913 4352 1391 Size: 802 1720 439 4072 Fragments arranged by size: 4072 1720 802 439 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2849 2910 928 Size: 971 950 61 5051 Fragments arranged by size: 5051 971 950 61 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5224 5258 1903 Size: 34 3287 34 3678 Fragments arranged by size: 3678 3287 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4925 6135 4925 Size: 1210 5823 BclI T'GATC_A Cuts at: 1548 2353 4009 1548 Size: 805 1656 4572 Fragments arranged by size: 4572 1656 805 BfrBI ATG'CAT Cuts at: 2204 2204 Size: 7033 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5644 136 Size: 107 122 71 631 605 3972 1525 Fragments arranged by size: 3972 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3694 7028 3694 Size: 3334 3699 BlpI GC'TnA_GC Cuts at: 4450 4450 Size: 7033 Bme1580I G_kGCm'C Cuts at: 1790 3763 5094 6250 1790 Size: 1973 1331 1156 2573 Fragments arranged by size: 2573 1973 1331 1156 BmgBI CAC'GTC Cuts at: 1318 3423 1318 Size: 2105 4928 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4764 5722 442 Size: 416 53 83 399 24 3347 958 1753 Fragments arranged by size: 3347 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7033 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2255 2255 Size: 7033 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3151 3656 5693 1239 Size: 1912 505 2037 2579 Fragments arranged by size: 2579 2037 1912 505 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5158 6023 6321 5158 Size: 865 298 5870 Fragments arranged by size: 5870 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2609 3177 5696 882 Size: 617 1110 568 2519 2219 Fragments arranged by size: 2519 2219 1110 617 568 BsaAI yAC'GTr Cuts at: 493 1204 4330 4824 493 Size: 711 3126 494 2702 Fragments arranged by size: 3126 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2072 7027 2072 Size: 4955 2078 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4840 5222 5857 275 Size: 53 83 186 936 3307 382 635 1451 Fragments arranged by size: 3307 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5466 5864 6138 6285 6696 5466 Size: 398 274 147 411 5803 Fragments arranged by size: 5803 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2538 2568 3149 3179 1178 Size: 30 165 30 1135 30 581 30 5032 Fragments arranged by size: 5032 1135 581 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 1186 Size: 1015 6018 BseYI C'CCAG_C Cuts at: 6236 6732 6236 Size: 496 6537 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6460 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 4483 5244 6272 1540 Size: 275 186 1081 1401 761 1028 2301 Fragments arranged by size: 2301 1401 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 3546 3763 5094 5179 6250 2193 Size: 1353 217 1331 85 1071 2976 Fragments arranged by size: 2976 1353 1331 1071 217 85 BsmI GAATG_Cn' Cuts at: 4544 4637 4544 Size: 93 6940 BsmBI CGTCTCn'nnnn_ Cuts at: 3119 3119 Size: 7033 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 3546 3763 3913 4352 5094 5179 Size: 399 403 1353 217 150 439 742 85 Cuts at: 5179 6250 1391 Size: 1071 2174 Fragments arranged by size: 2174 1353 1071 742 439 403 399 217 150 85 BspEI T'CCGG_A Cuts at: 6696 6696 Size: 7033 BspHI T'CATG_A Cuts at: 4922 6652 4922 Size: 1730 5303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4388 844 Size: 3544 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2539 4920 1394 Size: 429 716 2381 3507 Fragments arranged by size: 3507 2381 716 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3273 5528 5702 65 Size: 1183 2025 2255 174 1396 Fragments arranged by size: 2255 2025 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5677 5864 1700 Size: 110 3867 187 2869 Fragments arranged by size: 3867 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2394 2413 2919 96 Size: 2298 19 506 4210 Fragments arranged by size: 4210 2298 506 19 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7033 BssSI C'ACGA_G Cuts at: 5093 6105 5093 Size: 1012 6021 BstAPI GCAn_nnn'nTGC Cuts at: 794 3796 794 Size: 3002 4031 BstBI TT'CG_AA Cuts at: 2007 5837 2007 Size: 3830 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7033 BstYI r'GATC_y Cuts at: 1972 2073 3061 3694 3737 4363 4730 5115 5132 Size: 101 988 633 43 626 367 385 17 Cuts at: 5132 6584 6670 6682 7028 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 988 633 626 385 367 346 101 86 43 17 12 BstZ17I GTA'TAC Cuts at: 4312 4312 Size: 7033 Bsu36I CC'TnA_GG Cuts at: 4296 4296 Size: 7033 BtgI C'CryG_G Cuts at: 513 1065 2094 513 Size: 552 1029 5452 Fragments arranged by size: 5452 1029 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6294 Fragments arranged by size: 6294 683 56 BtsI GCAGTG_nn' Cuts at: 2770 4549 5343 5363 2770 Size: 1779 794 20 4440 Fragments arranged by size: 4440 1779 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7033 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3634 3669 529 Size: 35 3070 35 3893 Fragments arranged by size: 3893 3070 35 35 DraI TTT'AAA Cuts at: 3121 4337 3121 Size: 1216 5817 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6040 809 Size: 507 4724 1802 Fragments arranged by size: 4724 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4480 5369 6998 Size: 54 1425 50 275 18 2650 889 1629 Cuts at: 6998 8 Size: 43 Fragments arranged by size: 2650 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4480 1537 Size: 275 2668 4090 Fragments arranged by size: 4090 2668 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3062 4963 1353 Size: 1709 1901 3423 Fragments arranged by size: 3423 1901 1709 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5620 5994 6140 387 Size: 1064 138 4031 374 146 1280 Fragments arranged by size: 4031 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2191 2191 Size: 7033 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2697 3033 3086 3151 3656 3762 5096 Size: 393 1065 336 53 65 505 106 1334 Cuts at: 5096 5693 6480 6748 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1334 1065 787 597 505 393 336 268 106 65 53 EcoNI CCTnn'n_nnAGG Cuts at: 2983 2983 Size: 7033 EcoO109I rG'GnC_Cy Cuts at: 2125 2125 Size: 7033 EcoRI G'AATT_C Cuts at: 2863 3479 4342 4720 2863 Size: 616 863 378 5176 Fragments arranged by size: 5176 863 616 378 EcoRV GAT'ATC Cuts at: 3387 3886 3387 Size: 499 6534 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2595 3630 2595 Size: 1035 5998 FspI TGC'GCA Cuts at: 5539 5539 Size: 7033 HaeII r_GCGC'y Cuts at: 1678 6180 1678 Size: 4502 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2587 2619 5674 5706 5748 Size: 32 135 32 1015 32 3055 32 42 Cuts at: 5748 5780 1373 Size: 32 2626 Fragments arranged by size: 3055 2626 1015 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3040 3659 4377 4623 669 Size: 988 281 1102 619 718 246 3079 Fragments arranged by size: 3079 1102 988 718 619 281 246 HindIII A'AGCT_T Cuts at: 748 2343 748 Size: 1595 5438 HpaI GTT'AAC Cuts at: 4623 4623 Size: 7033 KpnI G_GTAC'C Cuts at: 4358 4358 Size: 7033 MfeI C'AATT_G Cuts at: 2799 4632 2799 Size: 1833 5200 MluI A'CGCG_T Cuts at: 6909 6909 Size: 7033 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3733 4098 6147 6331 1305 Size: 2428 365 2049 184 2007 Fragments arranged by size: 2428 2049 2007 365 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5211 Fragments arranged by size: 5211 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2066 2226 2390 3470 3583 3827 4991 Size: 648 900 160 164 1080 113 244 1164 Cuts at: 4991 5350 5509 518 Size: 359 159 2042 Fragments arranged by size: 2042 1164 1080 900 648 359 244 164 160 159 113 MspA1I CmG'CkG Cuts at: 1606 3946 4449 5126 6274 6519 1606 Size: 2340 503 677 1148 245 2120 Fragments arranged by size: 2340 2120 1148 677 503 245 NcoI C'CATG_G Cuts at: 513 1065 2094 513 Size: 552 1029 5452 Fragments arranged by size: 5452 1029 552 NdeI CA'TA_TG Cuts at: 387 2880 3681 4318 387 Size: 2493 801 637 3102 Fragments arranged by size: 3102 2493 801 637 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7033 NotI GC'GGCC_GC Cuts at: 4480 4480 Size: 7033 NsiI A_TGCA'T Cuts at: 2206 2206 Size: 7033 NspI r_CATG'y Cuts at: 1132 1229 2920 3614 4391 5936 1132 Size: 97 1691 694 777 1545 2229 Fragments arranged by size: 2229 1691 1545 777 694 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7033 PciI A'CATG_T Cuts at: 5932 5932 Size: 7033 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7033 PfoI T'CCnGG_A Cuts at: 3090 3090 Size: 7033 PmeI GTTT'AAAC Cuts at: 4337 4337 Size: 7033 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5102 5134 6615 6647 5102 Size: 32 1481 32 5488 Fragments arranged by size: 5488 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2125 Size: 7033 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7033 PsiI TTA'TAA Cuts at: 3318 4603 3318 Size: 1285 5748 PstI C_TGCA'G Cuts at: 830 2665 3694 4385 830 Size: 1835 1029 691 3478 Fragments arranged by size: 3478 1835 1029 691 PvuI CG_AT'CG Cuts at: 2001 3082 2001 Size: 1081 5952 SacI G_AGCT'C Cuts at: 2193 2193 Size: 7033 SalI G'TCGA_C Cuts at: 1936 3657 4375 1936 Size: 1721 718 4594 Fragments arranged by size: 4594 1721 718 SbfI CC_TGCA'GG Cuts at: 4385 4385 Size: 7033 ScaI AGT'ACT Cuts at: 5281 6993 5281 Size: 1712 5321 SfcI C'TryA_G Cuts at: 826 1045 2194 2661 2847 3494 3690 3914 4140 Size: 219 1149 467 186 647 196 224 226 Cuts at: 4140 4381 5516 6197 6388 826 Size: 241 1135 681 191 1471 Fragments arranged by size: 1471 1149 1135 681 647 467 241 226 224 219 196 191 186 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7033 SmaI CCC'GGG Cuts at: 1872 4360 1872 Size: 2488 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5137 6038 6300 820 Size: 197 926 3194 901 262 1553 Fragments arranged by size: 3194 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4330 4824 493 Size: 3837 494 2702 Fragments arranged by size: 3837 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7033 SphI G_CATG'C Cuts at: 1132 4391 1132 Size: 3259 3774 SspI AAT'ATT Cuts at: 5 52 3255 4280 4957 5 Size: 47 3203 1025 677 2081 Fragments arranged by size: 3203 2081 1025 677 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7033 StyI C'CwwG_G Cuts at: 513 1065 2094 2908 4472 513 Size: 552 1029 814 1564 3074 Fragments arranged by size: 3074 1564 1029 814 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2475 5060 5077 5230 5415 5860 325 Size: 1255 895 2585 17 153 185 445 1498 Fragments arranged by size: 2585 1498 1255 895 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2299 2394 2413 Size: 274 80 33 51 1443 322 95 19 Cuts at: 2413 2875 2919 5279 6991 96 Size: 462 44 2360 1712 138 Fragments arranged by size: 2360 1712 1443 462 322 274 138 95 80 51 44 33 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6993 7025 6993 Size: 32 7001 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7033 XbaI T'CTAG_A Cuts at: 4160 4369 4160 Size: 209 6824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7033 XmaI C'CCGG_G Cuts at: 1870 4358 1870 Size: 2488 4545 XmnI GAAnn'nnTTC Cuts at: 2670 5162 2670 Size: 2492 4541 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4841 276 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstXI DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI