(Circular) (Six-base) MAPSORT of: pFN21ASDA0608.seq Check: 2365 from: 1 to: 7036 pFN21ASDA0608 7036 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2877 2877 Size: 7036 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4846 278 Size: 53 83 186 936 3310 2468 Fragments arranged by size: 3310 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4379 1937 Size: 2442 4594 Acc65I G'GTAC_C Cuts at: 4357 4357 Size: 7036 AclI AA'CG_TT Cuts at: 1563 5163 5536 1563 Size: 3600 373 3063 Fragments arranged by size: 3600 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 3056 5099 6483 6751 1632 Size: 566 858 2043 1384 268 1917 Fragments arranged by size: 2043 1917 1384 858 566 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6839 AflIII A'CryG_T Cuts at: 1203 2829 5935 6912 1203 Size: 1626 3106 977 1327 Fragments arranged by size: 3106 1626 1327 977 AgeI A'CCGG_T Cuts at: 5867 5867 Size: 7036 AhdI GACnn_n'nnGTC Cuts at: 5765 5765 Size: 7036 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7036 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2934 3757 1184 Size: 1750 823 4463 Fragments arranged by size: 4463 1750 823 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4193 4225 4193 Size: 32 7004 AlwNI CAG_nnn'CTG Cuts at: 2682 4779 6351 2682 Size: 2097 1572 3367 Fragments arranged by size: 3367 2097 1572 ApaLI G'TGCA_C Cuts at: 5093 6249 5093 Size: 1156 5880 ApoI r'AATT_y Cuts at: 1493 3160 3167 4085 4345 4571 4723 6832 1493 Size: 1667 7 918 260 226 152 2109 1697 Fragments arranged by size: 2109 1697 1667 918 260 226 152 7 AscI GG'CGCG_CC Cuts at: 2742 2742 Size: 7036 AseI AT'TA_AT Cuts at: 160 1032 3641 5590 160 Size: 872 2609 1949 1606 Fragments arranged by size: 2609 1949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7036 AvaI C'yCGr_G Cuts at: 1870 1943 2053 2341 2416 2639 2659 2732 2934 Size: 73 110 288 75 223 20 73 202 Cuts at: 2934 4361 1870 Size: 1427 4545 Fragments arranged by size: 4545 1427 288 223 202 110 75 73 73 20 BamHI G'GATC_C Cuts at: 4366 4733 4366 Size: 367 6669 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 2291 2720 2890 4357 Size: 325 217 627 293 211 429 170 1467 Cuts at: 4357 5812 618 Size: 1455 1842 Fragments arranged by size: 1842 1467 1455 627 429 325 293 217 211 170 BanII G_rGCy'C Cuts at: 1391 2259 2342 2348 2640 2941 4355 1391 Size: 868 83 6 292 301 1414 4072 Fragments arranged by size: 4072 1414 868 301 292 83 6 BbeI G_GCGC'C Cuts at: 2295 2724 2295 Size: 429 6607 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3138 928 Size: 971 1239 4826 Fragments arranged by size: 4826 1239 971 BbvCI CC'TCA_GC Cuts at: 3282 3282 Size: 7036 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5227 5261 1903 Size: 34 3290 34 3678 Fragments arranged by size: 3678 3290 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4928 6138 4928 Size: 1210 5826 BclI T'GATC_A Cuts at: 1548 1548 Size: 7036 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2203 2289 2337 Size: 107 122 71 631 605 531 86 48 Cuts at: 2337 2511 2759 5647 136 Size: 174 248 2888 1525 Fragments arranged by size: 2888 1525 631 605 531 248 174 122 107 86 71 48 BglII A'GATC_T Cuts at: 7031 7031 Size: 7036 BlpI GC'TnA_GC Cuts at: 4453 4453 Size: 7036 Bme1580I G_kGCm'C Cuts at: 1790 2895 5097 6253 1790 Size: 1105 2202 1156 2573 Fragments arranged by size: 2573 2202 1156 1105 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7036 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4767 5725 442 Size: 416 53 83 399 24 3350 958 1753 Fragments arranged by size: 3350 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7036 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 2546 5696 1239 Size: 1028 279 3150 2579 Fragments arranged by size: 3150 2579 1028 279 Bpu10I CC'TnA_GC Cuts at: 3282 3282 Size: 7036 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2609 3221 3529 5161 6026 6324 2609 Size: 612 308 1632 865 298 3321 Fragments arranged by size: 3321 1632 865 612 308 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5699 882 Size: 617 4200 2219 Fragments arranged by size: 4200 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4333 4827 493 Size: 711 3129 494 2702 Fragments arranged by size: 3129 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7030 7030 Size: 7036 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 2195 2292 2325 Size: 53 83 186 936 615 47 97 33 Cuts at: 2325 2628 2721 4843 5225 5860 275 Size: 303 93 2122 382 635 1451 Fragments arranged by size: 2122 1451 936 635 615 382 303 186 97 93 83 53 47 33 BsaWI w'CCGG_w Cuts at: 2032 5469 5867 6141 6288 6699 2032 Size: 3437 398 274 147 411 2369 Fragments arranged by size: 3437 2369 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3112 3142 1178 Size: 30 165 30 1709 30 5072 Fragments arranged by size: 5072 1709 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2198 2201 2204 2750 1186 Size: 1000 12 3 3 546 5472 Fragments arranged by size: 5472 1000 546 12 3 3 BseYI C'CCAG_C Cuts at: 2335 6239 6735 2335 Size: 3904 496 2636 Fragments arranged by size: 3904 2636 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6463 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2235 2321 2485 2515 2763 4486 Size: 275 186 234 86 164 30 248 1723 Cuts at: 4486 5247 6275 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1723 1028 761 275 248 234 186 164 86 30 BsiHKAI G_wGCw'C Cuts at: 3028 5097 5182 6253 3028 Size: 2069 85 1071 3811 Fragments arranged by size: 3811 2069 1071 85 BsmI GAATG_Cn' Cuts at: 4547 4640 4547 Size: 93 6943 BsmBI CGTCTCn'nnnn_ Cuts at: 2186 2186 Size: 7036 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 2342 2348 2640 2895 2941 3028 Size: 399 469 83 6 292 255 46 87 Cuts at: 3028 4355 5097 5182 6253 1391 Size: 1327 742 85 1071 2174 Fragments arranged by size: 2174 1327 1071 742 469 399 292 255 87 85 83 46 6 BspEI T'CCGG_A Cuts at: 2032 6699 2032 Size: 4667 2369 BspHI T'CATG_A Cuts at: 3330 3706 4925 6655 3330 Size: 376 1219 1730 3711 Fragments arranged by size: 3711 1730 1219 376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2877 4391 844 Size: 2033 1514 3489 Fragments arranged by size: 3489 2033 1514 BsrBI CCG'CTC Cuts at: 1394 1823 2272 4923 1394 Size: 429 449 2651 3507 Fragments arranged by size: 3507 2651 449 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3378 3783 5531 5705 65 Size: 1183 2130 405 1748 174 1396 Fragments arranged by size: 2130 1748 1396 1183 405 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2288 2477 2887 5680 5867 1700 Size: 110 478 189 410 2793 187 2869 Fragments arranged by size: 2869 2793 478 410 189 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7036 BssHII G'CGCG_C Cuts at: 1925 2382 2471 2473 2742 1925 Size: 457 89 2 269 6219 Fragments arranged by size: 6219 457 269 89 2 BssSI C'ACGA_G Cuts at: 5096 6108 5096 Size: 1012 6024 BstAPI GCAn_nnn'nTGC Cuts at: 794 3457 794 Size: 2663 4373 BstBI TT'CG_AA Cuts at: 2007 5840 2007 Size: 3833 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7036 BstXI CCAn_nnnn'nTGG Cuts at: 3235 3235 Size: 7036 BstYI r'GATC_y Cuts at: 1972 4005 4366 4733 5118 5135 6587 6673 6685 Size: 2033 361 367 385 17 1452 86 12 Cuts at: 6685 7031 1972 Size: 346 1977 Fragments arranged by size: 2033 1977 1452 385 367 361 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2097 2127 2356 2387 2622 2845 Size: 552 957 75 30 229 31 235 223 Cuts at: 2845 2904 3472 513 Size: 59 568 4077 Fragments arranged by size: 4077 957 568 552 235 229 223 75 59 31 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6297 Fragments arranged by size: 6297 683 56 BtsI GCAGTG_nn' Cuts at: 3186 4552 5346 5366 3186 Size: 1366 794 20 4856 Fragments arranged by size: 4856 1366 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7036 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7001 DraI TTT'AAA Cuts at: 4340 4340 Size: 7036 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6043 809 Size: 507 4727 1802 Fragments arranged by size: 4727 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2281 2377 Size: 54 1425 50 275 18 402 49 96 Cuts at: 2377 2482 2512 2619 2760 4483 5372 7001 8 Size: 105 30 107 141 1723 889 1629 43 Fragments arranged by size: 1723 1629 1425 889 402 275 141 107 105 96 54 50 49 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2232 2482 2512 2760 4483 1537 Size: 275 420 250 30 248 1723 4090 Fragments arranged by size: 4090 1723 420 275 250 248 30 EarI CTCTTCn'nnn_ Cuts at: 1353 2448 3424 3807 4966 1353 Size: 1095 976 383 1159 3423 Fragments arranged by size: 3423 1159 1095 976 383 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2157 5623 5997 6143 387 Size: 1064 138 568 3466 374 146 1280 Fragments arranged by size: 3466 1280 1064 568 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2267 2546 3056 5099 5696 6483 Size: 393 566 69 279 510 2043 597 787 Cuts at: 6483 6751 1239 Size: 268 1524 Fragments arranged by size: 2043 1524 787 597 566 510 393 279 268 69 EcoO109I rG'GnC_Cy Cuts at: 2531 2593 2729 2915 3123 2531 Size: 62 136 186 208 6444 Fragments arranged by size: 6444 208 186 136 62 EcoRI G'AATT_C Cuts at: 4345 4723 4345 Size: 378 6658 EcoRV GAT'ATC Cuts at: 4192 4192 Size: 7036 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3956 3956 Size: 7036 FspI TGC'GCA Cuts at: 5542 5542 Size: 7036 HaeII r_GCGC'y Cuts at: 1678 2157 2295 2724 2859 6183 1678 Size: 479 138 429 135 3324 2531 Fragments arranged by size: 3324 2531 479 429 138 135 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2185 2217 2564 2596 2833 Size: 32 135 32 613 32 347 32 237 Cuts at: 2833 2865 3197 3229 4095 4127 4181 4213 5677 Size: 32 332 32 866 32 54 32 1464 Cuts at: 5677 5709 5751 5783 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1464 866 613 347 332 237 135 54 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4380 4626 669 Size: 988 281 2442 246 3079 Fragments arranged by size: 3079 2442 988 281 246 HindIII A'AGCT_T Cuts at: 748 3243 748 Size: 2495 4541 HpaI GTT'AAC Cuts at: 4626 4626 Size: 7036 KasI G'GCGC_C Cuts at: 2291 2720 2291 Size: 429 6607 KpnI G_GTAC'C Cuts at: 4361 4361 Size: 7036 MfeI C'AATT_G Cuts at: 3403 4635 3403 Size: 1232 5804 MluI A'CGCG_T Cuts at: 6912 6912 Size: 7036 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3014 3630 6150 6334 1305 Size: 1709 616 2520 184 2007 Fragments arranged by size: 2520 2007 1709 616 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2283 10 Size: 54 1425 343 451 4763 Fragments arranged by size: 4763 1425 451 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3739 4232 4994 5353 5512 518 Size: 648 2573 493 762 359 159 2042 Fragments arranged by size: 2573 2042 762 648 493 359 159 MspA1I CmG'CkG Cuts at: 1606 2245 2279 2358 2389 2599 2624 2682 2710 Size: 639 34 79 31 210 25 58 28 Cuts at: 2710 2847 2906 4452 5129 6277 6522 1606 Size: 137 59 1546 677 1148 245 2120 Fragments arranged by size: 2120 1546 1148 677 639 245 210 137 79 59 58 34 31 28 25 NaeI GCC'GGC Cuts at: 2290 2479 2290 Size: 189 6847 NarI GG'CG_CC Cuts at: 2292 2721 2292 Size: 429 6607 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5527 Fragments arranged by size: 5527 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7036 NgoMIV G'CCGG_C Cuts at: 2288 2477 2288 Size: 189 6847 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7036 NotI GC'GGCC_GC Cuts at: 4483 4483 Size: 7036 NruI TCG'CGA Cuts at: 2445 2445 Size: 7036 NspI r_CATG'y Cuts at: 1132 1229 3744 4394 5939 1132 Size: 97 2515 650 1545 2229 Fragments arranged by size: 2515 2229 1545 650 97 PacI TTA_AT'TAA Cuts at: 3645 3645 Size: 7036 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7036 PciI A'CATG_T Cuts at: 5935 5935 Size: 7036 PflMI CCAn_nnn'nTGG Cuts at: 1108 4254 1108 Size: 3146 3890 PmeI GTTT'AAAC Cuts at: 4340 4340 Size: 7036 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5105 5137 6618 6650 5105 Size: 32 1481 32 5491 Fragments arranged by size: 5491 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2593 2729 2915 3123 2593 Size: 136 186 208 6506 Fragments arranged by size: 6506 208 186 136 PshAI GACnn'nnGTC Cuts at: 1613 4262 1613 Size: 2649 4387 PsiI TTA'TAA Cuts at: 4606 4606 Size: 7036 PstI C_TGCA'G Cuts at: 830 2687 4143 4388 830 Size: 1857 1456 245 3478 Fragments arranged by size: 3478 1857 1456 245 PvuI CG_AT'CG Cuts at: 2001 2321 2001 Size: 320 6716 PvuII CAG'CTG Cuts at: 2245 2682 2245 Size: 437 6599 SacII CC_GC'GG Cuts at: 2359 2390 2625 2848 2907 2359 Size: 31 235 223 59 6488 Fragments arranged by size: 6488 235 223 59 31 SalI G'TCGA_C Cuts at: 1936 4378 1936 Size: 2442 4594 SanDI GG'GwC_CC Cuts at: 2729 2729 Size: 7036 SbfI CC_TGCA'GG Cuts at: 4388 4388 Size: 7036 ScaI AGT'ACT Cuts at: 3795 5284 6996 3795 Size: 1489 1712 3835 Fragments arranged by size: 3835 1712 1489 SfcI C'TryA_G Cuts at: 826 1045 2683 3495 3745 4139 4384 5519 6200 Size: 219 1638 812 250 394 245 1135 681 Cuts at: 6200 6391 826 Size: 191 1471 Fragments arranged by size: 1638 1471 1135 812 681 394 250 245 219 191 SfoI GGC'GCC Cuts at: 2293 2722 2293 Size: 429 6607 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7036 SgrAI Cr'CCGG_yG Cuts at: 1700 2477 2887 1700 Size: 777 410 5849 Fragments arranged by size: 5849 777 410 SmaI CCC'GGG Cuts at: 1872 2936 4363 1872 Size: 1064 1427 4545 Fragments arranged by size: 4545 1427 1064 SmlI C'TyrA_G Cuts at: 820 1017 1943 2588 3200 3544 5140 6041 6303 Size: 197 926 645 612 344 1596 901 262 Cuts at: 6303 820 Size: 1553 Fragments arranged by size: 1596 1553 926 901 645 612 344 262 197 SnaBI TAC'GTA Cuts at: 493 4333 4827 493 Size: 3840 494 2702 Fragments arranged by size: 3840 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7036 SphI G_CATG'C Cuts at: 1132 4394 1132 Size: 3262 3774 SrfI GCCC'GGGC Cuts at: 2936 2936 Size: 7036 SspI AAT'ATT Cuts at: 5 52 3361 3905 4960 5 Size: 47 3309 544 1055 2081 Fragments arranged by size: 3309 2081 1055 544 47 StuI AGG'CCT Cuts at: 1517 3751 1517 Size: 2234 4802 StyI C'CwwG_G Cuts at: 513 1065 2022 3769 4475 513 Size: 552 957 1747 706 3074 Fragments arranged by size: 3074 1747 957 706 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5063 5080 5233 5418 5863 325 Size: 1255 3483 17 153 185 445 1498 Fragments arranged by size: 3483 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3422 3793 5282 Size: 274 80 33 51 1443 1445 371 1489 Cuts at: 5282 6994 96 Size: 1712 138 Fragments arranged by size: 1712 1489 1445 1443 371 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6996 7028 6996 Size: 32 7004 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7036 XbaI T'CTAG_A Cuts at: 4372 4372 Size: 7036 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3026 3026 Size: 7036 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7036 XmaI C'CCGG_G Cuts at: 1870 2934 4361 1870 Size: 1064 1427 4545 Fragments arranged by size: 4545 1427 1064 XmnI GAAnn'nnTTC Cuts at: 3569 4131 5165 3569 Size: 562 1034 5440 Fragments arranged by size: 5440 1034 562 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4844 276 Size: 53 83 186 936 3310 2468 Fragments arranged by size: 3310 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NsiI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SwaI