(Circular) (Six-base) MAPSORT of: pFN21ASDA0604.seq Check: 5637 from: 1 to: 7024 pFN21ASDA0604 7024 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2204 2309 2502 2914 3127 2204 Size: 105 193 412 213 6101 Fragments arranged by size: 6101 412 213 193 105 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4834 278 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2727 4367 1937 Size: 790 1640 4594 Fragments arranged by size: 4594 1640 790 Acc65I G'GTAC_C Cuts at: 4345 4345 Size: 7024 AclI AA'CG_TT Cuts at: 1563 5151 5524 1563 Size: 3588 373 3063 Fragments arranged by size: 3588 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2507 3606 4052 5087 6471 6739 1632 Size: 875 1099 446 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1099 1035 875 446 268 AfeI AGC'GCT Cuts at: 2549 2549 Size: 7024 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6827 AflIII A'CryG_T Cuts at: 1203 3606 5923 6900 1203 Size: 2403 2317 977 1327 Fragments arranged by size: 2403 2317 1327 977 AgeI A'CCGG_T Cuts at: 5855 5855 Size: 7024 AhdI GACnn_n'nnGTC Cuts at: 5753 5753 Size: 7024 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7024 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7024 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3156 3188 3156 Size: 32 6992 AlwNI CAG_nnn'CTG Cuts at: 2044 2301 2463 2605 2929 3031 4000 4090 4767 Size: 257 162 142 324 102 969 90 677 Cuts at: 4767 6339 2044 Size: 1572 2729 Fragments arranged by size: 2729 1572 969 677 324 257 162 142 102 90 ApaI G_GGCC'C Cuts at: 2652 2652 Size: 7024 ApaLI G'TGCA_C Cuts at: 5081 6237 5081 Size: 1156 5868 ApoI r'AATT_y Cuts at: 1493 3579 3613 4333 4559 4711 6820 1493 Size: 2086 34 720 226 152 2109 1697 Fragments arranged by size: 2109 2086 1697 720 226 152 34 AseI AT'TA_AT Cuts at: 160 1032 5578 160 Size: 872 4546 1606 Fragments arranged by size: 4546 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 AvaI C'yCGr_G Cuts at: 1870 1943 2105 2802 4349 1870 Size: 73 162 697 1547 4545 Fragments arranged by size: 4545 1547 697 162 73 AvrII C'CTAG_G Cuts at: 2263 2263 Size: 7024 BamHI G'GATC_C Cuts at: 4354 4721 4354 Size: 367 6657 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 3034 4345 5800 618 Size: 325 217 627 429 818 1311 1455 1842 Fragments arranged by size: 1842 1455 1311 818 627 429 325 217 BanII G_rGCy'C Cuts at: 1391 2369 2599 2652 2790 3150 3546 4107 4175 Size: 978 230 53 138 360 396 561 68 Cuts at: 4175 4343 1391 Size: 168 4072 Fragments arranged by size: 4072 978 561 396 360 230 168 138 68 53 BbeI G_GCGC'C Cuts at: 3038 3038 Size: 7024 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2103 2546 3476 3730 928 Size: 971 204 443 930 254 4222 Fragments arranged by size: 4222 971 930 443 254 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5215 5249 1903 Size: 34 3278 34 3678 Fragments arranged by size: 3678 3278 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4916 6126 4916 Size: 1210 5814 BclI T'GATC_A Cuts at: 1548 3408 1548 Size: 1860 5164 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2431 3301 3895 Size: 107 122 71 631 605 759 870 594 Cuts at: 3895 4278 5635 136 Size: 383 1357 1525 Fragments arranged by size: 1525 1357 870 759 631 605 594 383 122 107 71 BglII A'GATC_T Cuts at: 2994 7019 2994 Size: 4025 2999 BlpI GC'TnA_GC Cuts at: 2532 4441 2532 Size: 1909 5115 Bme1580I G_kGCm'C Cuts at: 1790 2652 2967 4232 5085 6241 1790 Size: 862 315 1265 853 1156 2573 Fragments arranged by size: 2573 1265 1156 862 853 315 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7024 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2882 4755 5713 Size: 416 53 83 399 24 1465 1873 958 Cuts at: 5713 442 Size: 1753 Fragments arranged by size: 1873 1753 1465 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7024 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2363 2503 2951 3281 3560 5684 1239 Size: 980 144 140 448 330 279 2124 2579 Fragments arranged by size: 2579 2124 980 448 330 279 144 140 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3074 3202 5149 6014 6312 3074 Size: 128 1947 865 298 3786 Fragments arranged by size: 3786 1947 865 298 128 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2102 2277 2604 3257 3666 5687 882 Size: 617 603 175 327 653 409 2021 2219 Fragments arranged by size: 2219 2021 653 617 603 409 327 175 BsaAI yAC'GTr Cuts at: 493 1204 4321 4815 493 Size: 711 3117 494 2702 Fragments arranged by size: 3117 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7018 7018 Size: 7024 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2139 3035 3330 4831 Size: 53 83 186 936 606 896 295 1501 Cuts at: 4831 5213 5848 275 Size: 382 635 1451 Fragments arranged by size: 1501 1451 936 896 635 606 382 295 186 83 53 BsaWI w'CCGG_w Cuts at: 3420 3921 5457 5855 6129 6276 6687 3420 Size: 501 1536 398 274 147 411 3757 Fragments arranged by size: 3757 1536 501 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2375 2405 3935 3965 1178 Size: 30 165 30 972 30 1530 30 4237 Fragments arranged by size: 4237 1530 972 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2600 3002 3449 4094 1186 Size: 1414 402 447 645 4116 Fragments arranged by size: 4116 1414 645 447 402 BseYI C'CCAG_C Cuts at: 2044 2590 2598 2884 3665 4000 4225 6227 6723 Size: 546 8 286 781 335 225 2002 496 Cuts at: 6723 2044 Size: 2345 Fragments arranged by size: 2345 2002 781 546 496 335 286 225 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2220 3228 1163 Size: 573 484 1008 4959 Fragments arranged by size: 4959 1008 573 484 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3991 4474 5235 6263 1540 Size: 275 186 1990 483 761 1028 2301 Fragments arranged by size: 2301 1990 1028 761 483 275 186 BsiHKAI G_wGCw'C Cuts at: 2844 3150 4154 4175 5085 5170 6241 2844 Size: 306 1004 21 910 85 1071 3627 Fragments arranged by size: 3627 1071 1004 910 306 85 21 BsmI GAATG_Cn' Cuts at: 2319 3709 4535 4628 2319 Size: 1390 826 93 4715 Fragments arranged by size: 4715 1390 826 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2369 2599 2652 2790 2844 2967 3150 Size: 399 579 230 53 138 54 123 183 Cuts at: 3150 3546 4107 4154 4175 4232 4343 5085 5170 Size: 396 561 47 21 57 111 742 85 Cuts at: 5170 6241 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 579 561 399 396 230 183 138 123 111 85 57 54 53 47 21 BspEI T'CCGG_A Cuts at: 3420 3921 6687 3420 Size: 501 2766 3757 Fragments arranged by size: 3757 2766 501 BspHI T'CATG_A Cuts at: 4913 6643 4913 Size: 1730 5294 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2204 2309 2502 2914 3127 3317 3891 4379 Size: 1360 105 193 412 213 190 574 488 Cuts at: 4379 844 Size: 3489 Fragments arranged by size: 3489 1360 574 488 412 213 193 190 105 BsrBI CCG'CTC Cuts at: 1394 1823 4911 1394 Size: 429 3088 3507 Fragments arranged by size: 3507 3088 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2767 4011 5519 5693 65 Size: 1183 1519 1244 1508 174 1396 Fragments arranged by size: 1519 1508 1396 1244 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3378 5668 5855 1700 Size: 110 1568 2290 187 2869 Fragments arranged by size: 2869 2290 1568 187 110 BsrGI T'GTAC_A Cuts at: 96 3618 96 Size: 3522 3502 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7024 BssSI C'ACGA_G Cuts at: 2905 4178 5084 6096 2905 Size: 1273 906 1012 3833 Fragments arranged by size: 3833 1273 1012 906 BstAPI GCAn_nnn'nTGC Cuts at: 794 2926 4090 794 Size: 2132 1164 3728 Fragments arranged by size: 3728 2132 1164 BstBI TT'CG_AA Cuts at: 2007 5828 2007 Size: 3821 3203 BstEII G'GTnAC_C Cuts at: 1184 4189 1184 Size: 3005 4019 BstXI CCAn_nnnn'nTGG Cuts at: 2149 3553 3640 3971 2149 Size: 1404 87 331 5202 Fragments arranged by size: 5202 1404 331 87 BstYI r'GATC_y Cuts at: 1972 2994 4354 4721 5106 5123 6575 6661 6673 Size: 1022 1360 367 385 17 1452 86 12 Cuts at: 6673 7019 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1360 1022 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2142 3042 4098 513 Size: 552 1077 900 1056 3439 Fragments arranged by size: 3439 1077 1056 900 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6285 Fragments arranged by size: 6285 683 56 BtsI GCAGTG_nn' Cuts at: 2237 2530 2844 4540 5334 5354 2237 Size: 293 314 1696 794 20 3907 Fragments arranged by size: 3907 1696 794 314 293 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7024 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6989 DraI TTT'AAA Cuts at: 4328 4328 Size: 7024 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6031 809 Size: 507 4715 1802 Fragments arranged by size: 4715 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2949 3988 4471 Size: 54 1425 50 275 18 1119 1039 483 Cuts at: 4471 5360 6989 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1119 1039 889 483 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3988 4471 1537 Size: 275 2176 483 4090 Fragments arranged by size: 4090 2176 483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3362 4127 4954 1353 Size: 2009 765 827 3423 Fragments arranged by size: 3423 2009 827 765 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5611 5985 6131 387 Size: 1064 138 4022 374 146 1280 Fragments arranged by size: 4022 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3148 4173 3148 Size: 1025 5999 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2363 2503 2507 2951 3281 3560 Size: 393 587 144 140 4 444 330 279 Cuts at: 3560 3606 4052 5087 5684 6471 6739 1239 Size: 46 446 1035 597 787 268 1524 Fragments arranged by size: 1524 1035 787 597 587 446 444 393 330 279 268 144 140 46 4 EcoNI CCTnn'n_nnAGG Cuts at: 2309 2905 2309 Size: 596 6428 EcoO109I rG'GnC_Cy Cuts at: 2127 2648 2649 2697 3351 3439 3760 3790 2127 Size: 521 1 48 654 88 321 30 5361 Fragments arranged by size: 5361 654 521 321 88 48 30 1 EcoRI G'AATT_C Cuts at: 4333 4711 4333 Size: 378 6646 FspI TGC'GCA Cuts at: 5530 5530 Size: 7024 HaeII r_GCGC'y Cuts at: 1678 2551 3038 6171 1678 Size: 873 487 3133 2531 Fragments arranged by size: 3133 2531 873 487 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3047 3079 5665 5697 5739 Size: 32 135 32 1475 32 2586 32 42 Cuts at: 5739 5771 1373 Size: 32 2626 Fragments arranged by size: 2626 2586 1475 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3834 4368 4614 669 Size: 988 281 1896 534 246 3079 Fragments arranged by size: 3079 1896 988 534 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7024 HpaI GTT'AAC Cuts at: 4614 4614 Size: 7024 KasI G'GCGC_C Cuts at: 3034 3034 Size: 7024 KpnI G_GTAC'C Cuts at: 4349 4349 Size: 7024 MfeI C'AATT_G Cuts at: 4623 4623 Size: 7024 MluI A'CGCG_T Cuts at: 6900 6900 Size: 7024 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2615 2778 2916 3342 3743 4263 6138 6322 Size: 1310 163 138 426 401 520 1875 184 Cuts at: 6322 1305 Size: 2007 Fragments arranged by size: 2007 1875 1310 520 426 401 184 163 138 MscI TGG'CCA Cuts at: 10 64 1489 1832 2951 10 Size: 54 1425 343 1119 4083 Fragments arranged by size: 4083 1425 1119 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2324 2960 3812 4982 5341 5500 Size: 648 981 177 636 852 1170 359 159 Cuts at: 5500 518 Size: 2042 Fragments arranged by size: 2042 1170 981 852 648 636 359 177 159 MspA1I CmG'CkG Cuts at: 1606 2176 2197 2947 2977 3451 3746 4303 4440 Size: 570 21 750 30 474 295 557 137 Cuts at: 4440 5117 6265 6510 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 750 677 570 557 474 295 245 137 30 21 NarI GG'CG_CC Cuts at: 3035 3035 Size: 7024 NcoI C'CATG_G Cuts at: 513 1065 2142 3042 513 Size: 552 1077 900 4495 Fragments arranged by size: 4495 1077 900 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7024 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7024 NotI GC'GGCC_GC Cuts at: 4471 4471 Size: 7024 NspI r_CATG'y Cuts at: 1132 1229 3610 3842 4382 5927 1132 Size: 97 2381 232 540 1545 2229 Fragments arranged by size: 2381 2229 1545 540 232 97 PasI CC'CwG_GG Cuts at: 1348 2652 3442 4295 1348 Size: 1304 790 853 4077 Fragments arranged by size: 4077 1304 853 790 PciI A'CATG_T Cuts at: 3606 5923 3606 Size: 2317 4707 PflMI CCAn_nnn'nTGG Cuts at: 1108 3048 4000 1108 Size: 1940 952 4132 Fragments arranged by size: 4132 1940 952 PfoI T'CCnGG_A Cuts at: 2340 3537 2340 Size: 1197 5827 PmeI GTTT'AAAC Cuts at: 4328 4328 Size: 7024 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5093 5125 6606 6638 5093 Size: 32 1481 32 5479 Fragments arranged by size: 5479 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2697 3351 2697 Size: 654 6370 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7024 PsiI TTA'TAA Cuts at: 4594 4594 Size: 7024 PspOMI G'GGCC_C Cuts at: 2648 2648 Size: 7024 PstI C_TGCA'G Cuts at: 830 2040 3027 3759 4376 830 Size: 1210 987 732 617 3478 Fragments arranged by size: 3478 1210 987 732 617 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7024 PvuII CAG'CTG Cuts at: 2176 2197 2947 3451 4303 2176 Size: 21 750 504 852 4897 Fragments arranged by size: 4897 852 750 504 21 RsrII CG'GwC_CG Cuts at: 3423 4156 3423 Size: 733 6291 SacI G_AGCT'C Cuts at: 3150 4175 3150 Size: 1025 5999 SalI G'TCGA_C Cuts at: 1936 4366 1936 Size: 2430 4594 SanDI GG'GwC_CC Cuts at: 3351 3351 Size: 7024 SapI GCTCTTCn'nnn_ Cuts at: 4127 4127 Size: 7024 SbfI CC_TGCA'GG Cuts at: 3759 4376 3759 Size: 617 6407 ScaI AGT'ACT Cuts at: 5272 6984 5272 Size: 1712 5312 SexAI A'CCwGG_T Cuts at: 3198 3963 3198 Size: 765 6259 SfcI C'TryA_G Cuts at: 826 1045 2036 2567 2701 3023 3755 4372 5507 Size: 219 991 531 134 322 732 617 1135 Cuts at: 5507 6188 6379 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 991 732 681 617 531 322 219 191 134 SfoI GGC'GCC Cuts at: 3036 3036 Size: 7024 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7024 SmaI CCC'GGG Cuts at: 1872 4351 1872 Size: 2479 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3053 3217 5128 6029 6291 820 Size: 197 926 1110 164 1911 901 262 1553 Fragments arranged by size: 1911 1553 1110 926 901 262 197 164 SnaBI TAC'GTA Cuts at: 493 4321 4815 493 Size: 3828 494 2702 Fragments arranged by size: 3828 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7024 SphI G_CATG'C Cuts at: 1132 3842 4382 1132 Size: 2710 540 3774 Fragments arranged by size: 3774 2710 540 SspI AAT'ATT Cuts at: 5 52 4948 5 Size: 47 4896 2081 Fragments arranged by size: 4896 2081 47 StuI AGG'CCT Cuts at: 1517 2315 1517 Size: 798 6226 StyI C'CwwG_G Cuts at: 513 1065 2142 2263 3042 3334 3486 3633 3786 Size: 552 1077 121 779 292 152 147 153 Cuts at: 3786 3853 4463 513 Size: 67 610 3074 Fragments arranged by size: 3074 1077 779 610 552 292 153 152 147 121 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2370 2876 3243 4144 4208 5051 5068 Size: 1255 790 506 367 901 64 843 17 Cuts at: 5068 5221 5406 5851 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 901 843 790 506 445 367 185 153 64 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2070 3618 3951 Size: 274 80 33 51 1443 93 1548 333 Cuts at: 3951 5270 6982 96 Size: 1319 1712 138 Fragments arranged by size: 1712 1548 1443 1319 333 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6984 7016 6984 Size: 32 6992 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7024 XbaI T'CTAG_A Cuts at: 4360 4360 Size: 7024 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2393 2393 Size: 7024 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7024 XmaI C'CCGG_G Cuts at: 1870 4349 1870 Size: 2479 4545 XmnI GAAnn'nnTTC Cuts at: 2450 5153 2450 Size: 2703 4321 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4832 276 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacII SfiI SrfI SwaI