Restriction map for
pFN21ASDA0582
(Linear) (Six-base) MAPSORT of: KIAA0582.seq Check: 1303 from: 1 to: 2271
KIAA0582.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 1001 2271
Size: 1001 1270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 2062 2271
Size: 59 2003 209
Fragments arranged by size:
2003 209 59
AflII C'TTAA_G
Cuts at: 0 1123 2271
Size: 1123 1148
AgeI A'CCGG_T
Cuts at: 0 1793 2271
Size: 1793 478
AleI CACnn'nnGTG
Cuts at: 0 2258 2271
Size: 2258 13
AlwNI CAG_nnn'CTG
Cuts at: 0 402 489 2040 2085 2271
Size: 402 87 1551 45 186
Fragments arranged by size:
1551 402 186 87 45
ApaI G_GGCC'C
Cuts at: 0 107 1109 2271
Size: 107 1002 1162
Fragments arranged by size:
1162 1002 107
AvaI C'yCGr_G
Cuts at: 0 931 1738 2271
Size: 931 807 533
Fragments arranged by size:
931 807 533
BamHI G'GATC_C
Cuts at: 0 1498 1647 2271
Size: 1498 149 624
Fragments arranged by size:
1498 624 149
BanI G'GyrC_C
Cuts at: 0 1382 2271
Size: 1382 889
BanII G_rGCy'C
Cuts at: 0 107 152 234 269 559 1109 1209 1303
Size: 107 45 82 35 290 550 100 94
Cuts at: 1303 1552 2271
Size: 249 719
Fragments arranged by size:
719 550 290 249 107 100 94 82
45 35
BbeI G_GCGC'C
Cuts at: 0 1386 2271
Size: 1386 885
BbsI GAAGACnn'nnnn_
Cuts at: 0 47 370 1888 1979 2271
Size: 47 323 1518 91 292
Fragments arranged by size:
1518 323 292 91 47
BbvCI CC'TCA_GC
Cuts at: 0 140 496 938 2271
Size: 140 356 442 1333
Fragments arranged by size:
1333 442 356 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1429 1463 2271
Size: 1429 34 808
Fragments arranged by size:
1429 808 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 173 2271
Size: 173 2098
BglII A'GATC_T
Cuts at: 0 900 2271
Size: 900 1371
BlpI GC'TnA_GC
Cuts at: 0 553 572 2271
Size: 553 19 1699
Fragments arranged by size:
1699 553 19
Bme1580I G_kGCm'C
Cuts at: 0 107 1109 1380 1695 2271
Size: 107 1002 271 315 576
Fragments arranged by size:
1002 576 315 271 107
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 732 828 1538 2095 2271
Size: 48 684 96 710 557 176
Fragments arranged by size:
710 684 557 176 96 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1648 2271
Size: 1648 623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 604 703 1010 1122 1584 1643 2170
Size: 347 257 99 307 112 462 59 527
Cuts at: 2170 2271
Size: 101
Fragments arranged by size:
527 462 347 307 257 112 101 99
59
Bpu10I CC'TnA_GC
Cuts at: 0 140 432 496 516 938 1312 2271
Size: 140 292 64 20 422 374 959
Fragments arranged by size:
959 422 374 292 140 64 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1966 2271
Size: 1966 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 323 526 675 1749 2271
Size: 323 203 149 1074 522
Fragments arranged by size:
1074 522 323 203 149
BsaBI GATnn'nnATC
Cuts at: 0 2030 2271
Size: 2030 241
BsaHI Gr'CG_yC
Cuts at: 0 795 1383 2271
Size: 795 588 888
Fragments arranged by size:
888 795 588
BsaWI w'CCGG_w
Cuts at: 0 1793 2271
Size: 1793 478
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 850 880 2271
Size: 850 30 1391
Fragments arranged by size:
1391 850 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 663 666 1737 1859 2271
Size: 400 263 3 1071 122 412
Fragments arranged by size:
1071 412 400 263 122 3
BseYI C'CCAG_C
Cuts at: 0 1307 2271
Size: 1307 964
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1377 2196 2271
Size: 275 1102 819 75
Fragments arranged by size:
1102 819 275 75
BsiEI CG_ry'CG
Cuts at: 0 692 861 2271
Size: 692 169 1410
Fragments arranged by size:
1410 692 169
BsiHKAI G_wGCw'C
Cuts at: 0 2255 2271
Size: 2255 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 786 2271
Size: 786 1485
Bsp1286I G_dGCh'C
Cuts at: 0 107 152 234 269 559 1109 1209 1303
Size: 107 45 82 35 290 550 100 94
Cuts at: 1303 1380 1552 1695 2255 2271
Size: 77 172 143 560 16
Fragments arranged by size:
560 550 290 172 143 107 100 94
82 77 45 35 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 192 1399 2271
Size: 192 1207 872
Fragments arranged by size:
1207 872 192
BsrBI CCG'CTC
Cuts at: 0 84 1459 2271
Size: 84 1375 812
Fragments arranged by size:
1375 812 84
BsrDI GCAATG_nn'
Cuts at: 0 1879 2252 2271
Size: 1879 373 19
Fragments arranged by size:
1879 373 19
BsrFI r'CCGG_y
Cuts at: 0 687 1268 1793 2003 2271
Size: 687 581 525 210 268
Fragments arranged by size:
687 581 525 268 210
BssSI C'ACGA_G
Cuts at: 0 647 2271
Size: 647 1624
BstEII G'GTnAC_C
Cuts at: 0 625 743 1515 2271
Size: 625 118 772 756
Fragments arranged by size:
772 756 625 118
BstXI CCAn_nnnn'nTGG
Cuts at: 0 61 1642 2271
Size: 61 1581 629
Fragments arranged by size:
1581 629 61
BstYI r'GATC_y
Cuts at: 0 900 1498 1639 1647 2271
Size: 900 598 141 8 624
Fragments arranged by size:
900 624 598 141 8
Bsu36I CC'TnA_GG
Cuts at: 0 1252 1652 2271
Size: 1252 400 619
Fragments arranged by size:
1252 619 400
BtgI C'CryG_G
Cuts at: 0 317 1947 2237 2271
Size: 317 1630 290 34
Fragments arranged by size:
1630 317 290 34
BtsI GCAGTG_nn'
Cuts at: 0 1085 1135 2271
Size: 1085 50 1136
Fragments arranged by size:
1136 1085 50
DraIII CAC_nnn'GTG
Cuts at: 0 298 2271
Size: 298 1973
EaeI y'GGCC_r
Cuts at: 0 662 1798 2271
Size: 662 1136 473
Fragments arranged by size:
1136 662 473
EarI CTCTTCn'nnn_
Cuts at: 0 1354 1412 1898 2271
Size: 1354 58 486 373
Fragments arranged by size:
1354 486 373 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 655 2271
Size: 655 1616
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 347 604 703 1010 1122 1584 1643
Size: 59 288 257 99 307 112 462 59
Cuts at: 1643 2062 2170 2271
Size: 419 108 101
Fragments arranged by size:
462 419 307 288 257 112 108 101
99 59 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1341 2271
Size: 1341 930
EcoO109I rG'GnC_Cy
Cuts at: 0 103 727 1734 2271
Size: 103 624 1007 537
Fragments arranged by size:
1007 624 537 103
EcoRV GAT'ATC
Cuts at: 0 1536 2271
Size: 1536 735
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 922 1846 2271
Size: 922 924 425
Fragments arranged by size:
924 922 425
HaeII r_GCGC'y
Cuts at: 0 1386 2271
Size: 1386 885
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 137 169 448 480 850 882 1000 1032
Size: 137 32 279 32 370 32 118 32
Cuts at: 1032 1285 1317 2271
Size: 253 32 954
Fragments arranged by size:
954 370 279 253 137 118 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 370 2271
Size: 370 1901
KasI G'GCGC_C
Cuts at: 0 1382 2271
Size: 1382 889
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1769 2069 2271
Size: 1769 300 202
Fragments arranged by size:
1769 300 202
MscI TGG'CCA
Cuts at: 0 664 1800 2271
Size: 664 1136 471
Fragments arranged by size:
1136 664 471
MslI CAynn'nnrTG
Cuts at: 0 2258 2271
Size: 2258 13
MspA1I CmG'CkG
Cuts at: 0 584 973 1899 2271
Size: 584 389 926 372
Fragments arranged by size:
926 584 389 372
NarI GG'CG_CC
Cuts at: 0 1383 2271
Size: 1383 888
NcoI C'CATG_G
Cuts at: 0 317 2237 2271
Size: 317 1920 34
Fragments arranged by size:
1920 317 34
NspI r_CATG'y
Cuts at: 0 2202 2271
Size: 2202 69
PasI CC'CwG_GG
Cuts at: 0 6 100 2271
Size: 6 94 2171
Fragments arranged by size:
2171 94 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 489 1102 2271
Size: 489 613 1169
Fragments arranged by size:
1169 613 489
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 546 578 2271
Size: 546 32 1693
Fragments arranged by size:
1693 546 32
PpuMI rG'GwC_Cy
Cuts at: 0 727 2271
Size: 727 1544
PspOMI G'GGCC_C
Cuts at: 0 103 1105 2271
Size: 103 1002 1166
Fragments arranged by size:
1166 1002 103
PstI C_TGCA'G
Cuts at: 0 79 589 989 1959 1997 2271
Size: 79 510 400 970 38 274
Fragments arranged by size:
970 510 400 274 79 38
PvuII CAG'CTG
Cuts at: 0 584 973 1899 2271
Size: 584 389 926 372
Fragments arranged by size:
926 584 389 372
SapI GCTCTTCn'nnn_
Cuts at: 0 1898 2271
Size: 1898 373
SbfI CC_TGCA'GG
Cuts at: 0 989 2271
Size: 989 1282
ScaI AGT'ACT
Cuts at: 0 817 2271
Size: 817 1454
SfcI C'TryA_G
Cuts at: 0 75 585 985 1277 1955 1993 2271
Size: 75 510 400 292 678 38 278
Fragments arranged by size:
678 510 400 292 278 75 38
SfoI GGC'GCC
Cuts at: 0 1384 2271
Size: 1384 887
SmaI CCC'GGG
Cuts at: 0 933 2271
Size: 933 1338
SmlI C'TyrA_G
Cuts at: 0 1123 1981 2271
Size: 1123 858 290
Fragments arranged by size:
1123 858 290
SrfI GCCC'GGGC
Cuts at: 0 933 2271
Size: 933 1338
StyI C'CwwG_G
Cuts at: 0 317 665 1511 1553 1635 2118 2237 2271
Size: 317 348 846 42 82 483 119 34
Fragments arranged by size:
846 483 348 317 119 82 42 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2270 2271
Size: 2270 1
TatI w'GTAC_w
Cuts at: 0 815 2271
Size: 815 1456
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1315 1347 2271
Size: 1315 32 924
Fragments arranged by size:
1315 924 32
Tth111I GACn'n_nGTC
Cuts at: 0 979 2271
Size: 979 1292
XbaI T'CTAG_A
Cuts at: 0 226 2271
Size: 226 2045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1156 1509 1618 2244 2271
Size: 1156 353 109 626 27
Fragments arranged by size:
1156 626 353 109 27
XmaI C'CCGG_G
Cuts at: 0 931 2271
Size: 931 1340
Enzymes that do cut:
AccI AcuI AflII AgeI AleI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I
NarI NcoI NspI PasI PflMI PpiI PpuMI PspOMI
PstI PvuII SapI SbfI ScaI SfcI SfoI SmaI
SmlI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BclI BfrBI BglI BmgBI BmtI BsaAI BsiWI BsmI
BspEI BspHI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EcoICRI EcoRI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI XhoI
XmnI ZraI