(Circular) (Six-base) MAPSORT of: pFN21ASDA0558.seq Check: 1061 from: 1 to: 8164 pFN21ASDA0558 8164 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3457 3457 Size: 8164 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2135 3252 3327 5974 Size: 53 83 186 936 599 1117 75 2647 Cuts at: 5974 278 Size: 2468 Fragments arranged by size: 2647 2468 1117 936 599 186 83 75 53 AccI GT'mk_AC Cuts at: 1937 2265 5507 1937 Size: 328 3242 4594 Fragments arranged by size: 4594 3242 328 Acc65I G'GTAC_C Cuts at: 5485 5485 Size: 8164 AclI AA'CG_TT Cuts at: 1563 3845 6291 6664 1563 Size: 2282 2446 373 3063 Fragments arranged by size: 3063 2446 2282 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2444 2963 4062 4729 6227 7611 7879 1632 Size: 812 519 1099 667 1498 1384 268 1917 Fragments arranged by size: 1917 1498 1384 1099 812 667 519 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7967 AflIII A'CryG_T Cuts at: 1203 3483 3693 4048 4254 7063 8040 1203 Size: 2280 210 355 206 2809 977 1327 Fragments arranged by size: 2809 2280 1327 977 355 210 206 AgeI A'CCGG_T Cuts at: 6995 6995 Size: 8164 AhdI GACnn_n'nnGTC Cuts at: 2983 6893 2983 Size: 3910 4254 AleI CACnn'nnGTG Cuts at: 1166 2758 3902 4481 1166 Size: 1592 1144 579 4849 Fragments arranged by size: 4849 1592 1144 579 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8164 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2708 2740 2708 Size: 32 8132 AlwNI CAG_nnn'CTG Cuts at: 2422 3994 4219 4629 5140 5907 7479 2422 Size: 1572 225 410 511 767 1572 3107 Fragments arranged by size: 3107 1572 1572 767 511 410 225 ApaI G_GGCC'C Cuts at: 2142 2245 2142 Size: 103 8061 ApaLI G'TGCA_C Cuts at: 6221 7377 6221 Size: 1156 7008 ApoI r'AATT_y Cuts at: 1493 5473 5699 5851 7960 1493 Size: 3980 226 152 2109 1697 Fragments arranged by size: 3980 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6718 160 Size: 872 5686 1606 Fragments arranged by size: 5686 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8164 AvaI C'yCGr_G Cuts at: 1870 1943 3186 4302 4869 5018 5489 1870 Size: 73 1243 1116 567 149 471 4545 Fragments arranged by size: 4545 1243 1116 567 471 149 73 AvrII C'CTAG_G Cuts at: 4495 4495 Size: 8164 BamHI G'GATC_C Cuts at: 5494 5861 5494 Size: 367 7797 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2048 2117 2124 2195 Size: 325 217 627 242 19 69 7 71 Cuts at: 2195 3122 3904 5485 6940 618 Size: 927 782 1581 1455 1842 Fragments arranged by size: 1842 1581 1455 927 782 627 325 242 217 71 69 19 7 BanII G_rGCy'C Cuts at: 1391 2142 2245 2697 2824 4134 4353 4491 5018 Size: 751 103 452 127 1310 219 138 527 Cuts at: 5018 5483 1391 Size: 465 4072 Fragments arranged by size: 4072 1310 751 527 465 452 219 138 127 103 BbeI G_GCGC'C Cuts at: 2052 2128 2199 2052 Size: 76 71 8017 Fragments arranged by size: 8017 76 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2499 2761 2960 4300 4393 928 Size: 971 600 262 199 1340 93 4699 Fragments arranged by size: 4699 1340 971 600 262 199 93 BbvCI CC'TCA_GC Cuts at: 4326 4839 4326 Size: 513 7651 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6355 6389 1903 Size: 34 4418 34 3678 Fragments arranged by size: 4418 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6056 7266 6056 Size: 1210 6954 BclI T'GATC_A Cuts at: 1548 3207 1548 Size: 1659 6505 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5416 6775 136 Size: 107 122 71 631 605 3744 1359 1525 Fragments arranged by size: 3744 1525 1359 631 605 122 107 71 BglII A'GATC_T Cuts at: 8159 8159 Size: 8164 BlpI GC'TnA_GC Cuts at: 2526 4490 5581 2526 Size: 1964 1091 5109 Fragments arranged by size: 5109 1964 1091 Bme1580I G_kGCm'C Cuts at: 1790 2142 2245 3125 4837 4887 6225 7381 1790 Size: 352 103 880 1712 50 1338 1156 2573 Fragments arranged by size: 2573 1712 1338 1156 880 352 103 50 BmgBI CAC'GTC Cuts at: 1318 3607 1318 Size: 2289 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2248 3509 3515 Size: 416 53 83 399 24 831 1261 6 Cuts at: 3515 5050 5895 6853 442 Size: 1535 845 958 1753 Fragments arranged by size: 1753 1535 1261 958 845 831 416 399 83 53 24 6 BmtI G_CTAG'C Cuts at: 1056 4577 1056 Size: 3521 4643 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2273 2735 4134 4460 4466 5101 6824 1239 Size: 1034 462 1399 326 6 635 1723 2579 Fragments arranged by size: 2579 1723 1399 1034 635 462 326 6 Bpu10I CC'TnA_GC Cuts at: 4326 4347 4839 4326 Size: 21 492 7651 Fragments arranged by size: 7651 492 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3698 5412 6289 7154 7452 3698 Size: 1714 877 865 298 4410 Fragments arranged by size: 4410 1714 877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2851 5263 5383 6827 882 Size: 617 1352 2412 120 1444 2219 Fragments arranged by size: 2412 2219 1444 1352 617 120 BsaAI yAC'GTr Cuts at: 493 1204 3694 4257 5461 5955 493 Size: 711 2490 563 1204 494 2702 Fragments arranged by size: 2702 2490 1204 711 563 494 BsaBI GATnn'nnATC Cuts at: 8158 8158 Size: 8164 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2125 2132 2196 Size: 53 83 186 936 516 76 7 64 Cuts at: 2196 2248 2292 2511 3249 3324 4530 5370 5971 Size: 52 44 219 738 75 1206 840 601 Cuts at: 5971 6353 6988 275 Size: 382 635 1451 Fragments arranged by size: 1451 1206 936 840 738 635 601 516 382 219 186 83 76 75 64 53 52 44 7 BsaWI w'CCGG_w Cuts at: 2325 3276 5280 6597 6995 7269 7416 7827 2325 Size: 951 2004 1317 398 274 147 411 2662 Fragments arranged by size: 2662 2004 1317 951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2657 2687 2708 2738 4772 Size: 30 165 30 1254 30 21 30 2034 Cuts at: 4772 4802 5379 5409 1178 Size: 30 577 30 3933 Fragments arranged by size: 3933 2034 1254 577 165 30 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5387 1186 Size: 4201 3963 BseYI C'CCAG_C Cuts at: 2065 2217 4025 5233 5365 7367 7863 2065 Size: 152 1808 1208 132 2002 496 2366 Fragments arranged by size: 2366 2002 1808 1208 496 152 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 3080 3122 3263 3491 3686 4166 Size: 573 699 645 42 141 228 195 480 Cuts at: 4166 4848 5206 5285 1163 Size: 682 358 79 4042 Fragments arranged by size: 4042 699 682 645 573 480 358 228 195 141 79 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5420 5614 6375 7403 1540 Size: 275 186 3419 194 761 1028 2301 Fragments arranged by size: 3419 2301 1028 761 275 194 186 BsiHKAI G_wGCw'C Cuts at: 2697 3920 3945 4134 4491 6225 6310 7381 2697 Size: 1223 25 189 357 1734 85 1071 3480 Fragments arranged by size: 3480 1734 1223 1071 357 189 85 25 BsmI GAATG_Cn' Cuts at: 4093 5675 5768 4093 Size: 1582 93 6489 Fragments arranged by size: 6489 1582 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5015 5216 5015 Size: 201 7963 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2245 2697 2824 3125 3920 3945 Size: 399 352 103 452 127 301 795 25 Cuts at: 3945 4134 4353 4491 4837 4887 5018 5483 6225 Size: 189 219 138 346 50 131 465 742 Cuts at: 6225 6310 7381 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 795 742 465 452 399 352 346 301 219 189 138 131 127 103 85 50 25 BspEI T'CCGG_A Cuts at: 7827 7827 Size: 8164 BspHI T'CATG_A Cuts at: 3210 5043 6053 7783 3210 Size: 1833 1010 1730 3591 Fragments arranged by size: 3591 1833 1730 1010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2051 2755 3457 3988 4728 5519 844 Size: 1207 704 702 531 740 791 3489 Fragments arranged by size: 3489 1207 791 740 704 702 531 BsrBI CCG'CTC Cuts at: 1394 1823 2931 6051 1394 Size: 429 1108 3120 3507 Fragments arranged by size: 3507 3120 1108 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4056 6659 6833 65 Size: 1183 2808 2603 174 1396 Fragments arranged by size: 2808 2603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2032 3140 5205 6808 6995 1700 Size: 110 222 1108 2065 1603 187 2869 Fragments arranged by size: 2869 2065 1603 1108 222 187 110 BsrGI T'GTAC_A Cuts at: 96 2644 96 Size: 2548 5616 BssHII G'CGCG_C Cuts at: 1925 2078 1925 Size: 153 8011 BssSI C'ACGA_G Cuts at: 6224 7236 6224 Size: 1012 7152 BstAPI GCAn_nnn'nTGC Cuts at: 794 2230 4832 5193 5392 794 Size: 1436 2602 361 199 3566 Fragments arranged by size: 3566 2602 1436 361 199 BstBI TT'CG_AA Cuts at: 2007 6968 2007 Size: 4961 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8164 BstXI CCAn_nnnn'nTGG Cuts at: 2896 3445 2896 Size: 549 7615 BstYI r'GATC_y Cuts at: 1972 2649 3375 3795 3990 4246 5494 5861 6246 Size: 677 726 420 195 256 1248 367 385 Cuts at: 6246 6263 7715 7801 7813 8159 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1248 726 677 420 385 367 346 256 195 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2582 2582 Size: 8164 BtgI C'CryG_G Cuts at: 513 1065 2022 2813 3303 3438 4390 4998 5341 Size: 552 957 791 490 135 952 608 343 Cuts at: 5341 513 Size: 3336 Fragments arranged by size: 3336 957 952 791 608 552 490 343 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7425 Fragments arranged by size: 7425 683 56 BtsI GCAGTG_nn' Cuts at: 2753 2929 3344 3479 4169 4527 5227 5258 5680 Size: 176 415 135 690 358 700 31 422 Cuts at: 5680 6474 6494 2753 Size: 794 20 4423 Fragments arranged by size: 4423 794 700 690 422 415 358 176 135 31 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8164 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8129 DraI TTT'AAA Cuts at: 5468 5468 Size: 8164 DraIII CAC_nnn'GTG Cuts at: 2814 3058 2814 Size: 244 7920 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7171 809 Size: 507 5855 1802 Fragments arranged by size: 5855 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2805 3114 3269 Size: 54 1425 50 275 18 975 309 155 Cuts at: 3269 4269 5169 5203 5338 5417 5611 6500 8129 Size: 1000 900 34 135 79 194 889 1629 Cuts at: 8129 8 Size: 43 Fragments arranged by size: 1629 1425 1000 975 900 889 309 275 194 155 135 79 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 5417 5611 1537 Size: 275 3605 194 4090 Fragments arranged by size: 4090 3605 275 194 EarI CTCTTCn'nnn_ Cuts at: 1353 2420 3786 6094 1353 Size: 1067 1366 2308 3423 Fragments arranged by size: 3423 2308 1366 1067 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6751 7125 7271 387 Size: 1064 138 5162 374 146 1280 Fragments arranged by size: 5162 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2695 4132 4489 2695 Size: 1437 357 6370 Fragments arranged by size: 6370 1437 357 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2444 2735 2963 4062 4134 4460 Size: 393 641 171 291 228 1099 72 326 Cuts at: 4460 4466 4729 5101 6227 6824 7611 7879 1239 Size: 6 263 372 1126 597 787 268 1524 Fragments arranged by size: 1524 1126 1099 787 641 597 393 372 326 291 268 263 228 171 72 6 EcoNI CCTnn'n_nnAGG Cuts at: 2336 3059 3548 2336 Size: 723 489 6952 Fragments arranged by size: 6952 723 489 EcoO109I rG'GnC_Cy Cuts at: 2139 2419 2602 2710 2877 3525 4081 5346 2139 Size: 280 183 108 167 648 556 1265 4957 Fragments arranged by size: 4957 1265 648 556 280 183 167 108 EcoRI G'AATT_C Cuts at: 5473 5851 5473 Size: 378 7786 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3160 3160 Size: 8164 FspI TGC'GCA Cuts at: 3074 6670 3074 Size: 3596 4568 HaeII r_GCGC'y Cuts at: 1678 2052 2128 2199 2781 7311 1678 Size: 374 76 71 582 4530 2531 Fragments arranged by size: 4530 2531 582 374 76 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2969 3001 6805 6837 6879 Size: 32 135 32 1397 32 3804 32 42 Cuts at: 6879 6911 1373 Size: 32 2626 Fragments arranged by size: 3804 2626 1397 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2266 2626 4651 5508 5754 669 Size: 988 281 328 360 2025 857 246 3079 Fragments arranged by size: 3079 2025 988 857 360 328 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8164 HpaI GTT'AAC Cuts at: 4651 5754 4651 Size: 1103 7061 KasI G'GCGC_C Cuts at: 2048 2124 2195 2048 Size: 76 71 8017 Fragments arranged by size: 8017 76 71 KpnI G_GTAC'C Cuts at: 5489 5489 Size: 8164 MfeI C'AATT_G Cuts at: 3265 5763 3265 Size: 2498 5666 MluI A'CGCG_T Cuts at: 8040 8040 Size: 8164 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3201 3782 7278 7462 1305 Size: 1896 581 3496 184 2007 Fragments arranged by size: 3496 2007 1896 581 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3116 3271 10 Size: 54 1425 343 1284 155 4903 Fragments arranged by size: 4903 1425 1284 343 155 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2758 2906 3318 3605 3902 4395 Size: 648 861 731 148 412 287 297 493 Cuts at: 4395 4419 4481 6122 6481 6640 518 Size: 24 62 1641 359 159 2042 Fragments arranged by size: 2042 1641 861 731 648 493 412 359 297 287 159 148 62 24 MspA1I CmG'CkG Cuts at: 1606 2152 2179 2469 3166 3334 3580 4219 4325 Size: 546 27 290 697 168 246 639 106 Cuts at: 4325 4438 4481 4980 5000 5037 5182 5237 5343 Size: 113 43 499 20 37 145 55 106 Cuts at: 5343 5580 6257 7405 7650 1606 Size: 237 677 1148 245 2120 Fragments arranged by size: 2120 1148 697 677 639 546 499 290 246 245 237 168 145 113 106 106 55 43 37 27 20 NaeI GCC'GGC Cuts at: 2034 3142 5207 2034 Size: 1108 2065 4991 Fragments arranged by size: 4991 2065 1108 NarI GG'CG_CC Cuts at: 2049 2125 2196 2049 Size: 76 71 8017 Fragments arranged by size: 8017 76 71 NcoI C'CATG_G Cuts at: 513 1065 2022 3438 513 Size: 552 957 1416 5239 Fragments arranged by size: 5239 1416 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8164 NgoMIV G'CCGG_C Cuts at: 2032 3140 5205 2032 Size: 1108 2065 4991 Fragments arranged by size: 4991 2065 1108 NheI G'CTAG_C Cuts at: 1052 4573 1052 Size: 3521 4643 NotI GC'GGCC_GC Cuts at: 5611 5611 Size: 8164 NspI r_CATG'y Cuts at: 1132 1229 5522 7067 1132 Size: 97 4293 1545 2229 Fragments arranged by size: 4293 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3560 1348 Size: 2212 5952 PciI A'CATG_T Cuts at: 7063 7063 Size: 8164 PflMI CCAn_nnn'nTGG Cuts at: 1108 2814 1108 Size: 1706 6458 PfoI T'CCnGG_A Cuts at: 3735 3759 3735 Size: 24 8140 PmeI GTTT'AAAC Cuts at: 5468 5468 Size: 8164 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2708 2740 6233 6265 7746 7778 2708 Size: 32 3493 32 1481 32 3094 Fragments arranged by size: 3493 3094 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2602 3525 4081 2602 Size: 923 556 6685 Fragments arranged by size: 6685 923 556 PshAI GACnn'nnGTC Cuts at: 1613 2960 1613 Size: 1347 6817 PsiI TTA'TAA Cuts at: 5734 5734 Size: 8164 PspOMI G'GGCC_C Cuts at: 2138 2241 2138 Size: 103 8061 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3998 4030 3998 Size: 32 8132 PstI C_TGCA'G Cuts at: 830 2446 3171 3339 3999 5217 5516 830 Size: 1616 725 168 660 1218 299 3478 Fragments arranged by size: 3478 1616 1218 725 660 299 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8164 PvuII CAG'CTG Cuts at: 3166 3580 4219 4325 4481 4980 5037 3166 Size: 414 639 106 156 499 57 6293 Fragments arranged by size: 6293 639 499 414 156 106 57 SacI G_AGCT'C Cuts at: 2697 4134 4491 2697 Size: 1437 357 6370 Fragments arranged by size: 6370 1437 357 SacII CC_GC'GG Cuts at: 5001 5344 5001 Size: 343 7821 SalI G'TCGA_C Cuts at: 1936 2264 5506 1936 Size: 328 3242 4594 Fragments arranged by size: 4594 3242 328 SanDI GG'GwC_CC Cuts at: 3525 3525 Size: 8164 SbfI CC_TGCA'GG Cuts at: 3999 5516 3999 Size: 1517 6647 ScaI AGT'ACT Cuts at: 4073 5060 6412 8124 4073 Size: 987 1352 1712 4113 Fragments arranged by size: 4113 1712 1352 987 SexAI A'CCwGG_T Cuts at: 3054 4728 3054 Size: 1674 6490 SfcI C'TryA_G Cuts at: 826 1045 2206 2442 3167 3335 3925 3952 3995 Size: 219 1161 236 725 168 590 27 43 Cuts at: 3995 5213 5512 6647 7328 7519 826 Size: 1218 299 1135 681 191 1471 Fragments arranged by size: 1471 1218 1161 1135 725 681 590 299 236 219 191 168 43 27 SfoI GGC'GCC Cuts at: 2050 2126 2197 2050 Size: 76 71 8017 Fragments arranged by size: 8017 76 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8164 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8164 SmaI CCC'GGG Cuts at: 1872 4871 5491 1872 Size: 2999 620 4545 Fragments arranged by size: 4545 2999 620 SmlI C'TyrA_G Cuts at: 820 1017 1943 3713 5427 6268 7169 7431 820 Size: 197 926 1770 1714 841 901 262 1553 Fragments arranged by size: 1770 1714 1553 926 901 841 262 197 SnaBI TAC'GTA Cuts at: 493 5461 5955 493 Size: 4968 494 2702 Fragments arranged by size: 4968 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8164 SphI G_CATG'C Cuts at: 1132 5522 1132 Size: 4390 3774 SspI AAT'ATT Cuts at: 5 52 6088 5 Size: 47 6036 2081 Fragments arranged by size: 6036 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8164 StyI C'CwwG_G Cuts at: 513 1065 2022 2514 3117 3438 4495 5603 513 Size: 552 957 492 603 321 1057 1108 3074 Fragments arranged by size: 3074 1108 1057 957 603 552 492 321 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2902 3892 4847 6191 6208 6361 6546 Size: 1255 1322 990 955 1344 17 153 185 Cuts at: 6546 6991 325 Size: 445 1498 Fragments arranged by size: 1498 1344 1322 1255 990 955 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2502 2644 3261 Size: 274 80 33 51 1443 525 142 617 Cuts at: 3261 4071 5058 6410 8122 96 Size: 810 987 1352 1712 138 Fragments arranged by size: 1712 1443 1352 987 810 617 525 274 142 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3334 3366 4683 4715 8124 8156 3334 Size: 32 1317 32 3409 32 3342 Fragments arranged by size: 3409 3342 1317 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2897 1316 Size: 1581 6583 XbaI T'CTAG_A Cuts at: 3418 5500 3418 Size: 2082 6082 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4768 5198 4768 Size: 430 7734 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8164 XmaI C'CCGG_G Cuts at: 1870 4869 5489 1870 Size: 2999 620 4545 Fragments arranged by size: 4545 2999 620 XmnI GAAnn'nnTTC Cuts at: 2333 2723 3086 6293 2333 Size: 390 363 3207 4204 Fragments arranged by size: 4204 3207 390 363 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2133 3250 3325 5972 Size: 53 83 186 936 599 1117 75 2647 Cuts at: 5972 276 Size: 2468 Fragments arranged by size: 2647 2468 1117 936 599 186 83 75 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SapI SfiI SrfI SwaI