Restriction map for
pFN21ASDA0544
(Linear) (Six-base) MAPSORT of: KIAA0544.seq Check: 5852 from: 1 to: 1656
KIAA0544.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1605 1656
Size: 1605 51
AccI GT'mk_AC
Cuts at: 0 443 1656
Size: 443 1213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 1005 1656
Size: 167 838 651
Fragments arranged by size:
838 651 167
AhdI GACnn_n'nnGTC
Cuts at: 0 1266 1656
Size: 1266 390
AlwNI CAG_nnn'CTG
Cuts at: 0 1044 1656
Size: 1044 612
ApaI G_GGCC'C
Cuts at: 0 966 1628 1656
Size: 966 662 28
Fragments arranged by size:
966 662 28
ApoI r'AATT_y
Cuts at: 0 127 1656
Size: 127 1529
AvaI C'yCGr_G
Cuts at: 0 341 1174 1532 1573 1656
Size: 341 833 358 41 83
Fragments arranged by size:
833 358 341 83 41
BanII G_rGCy'C
Cuts at: 0 7 239 460 966 1005 1163 1628 1656
Size: 7 232 221 506 39 158 465 28
Fragments arranged by size:
506 465 232 221 158 39 28 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 250 1367 1656
Size: 250 1117 289
Fragments arranged by size:
1117 289 250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 395 1656
Size: 361 34 1261
Fragments arranged by size:
1261 361 34
BglI GCCn_nnn'nGGC
Cuts at: 0 388 1102 1328 1656
Size: 388 714 226 328
Fragments arranged by size:
714 388 328 226
BglII A'GATC_T
Cuts at: 0 755 1656
Size: 755 901
Bme1580I G_kGCm'C
Cuts at: 0 966 1628 1656
Size: 966 662 28
Fragments arranged by size:
966 662 28
BmgBI CAC'GTC
Cuts at: 0 1419 1656
Size: 1419 237
BmrI ACTGGGnnnn_n'
Cuts at: 0 1447 1656
Size: 1447 209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1182 1425 1656
Size: 1182 243 231
Fragments arranged by size:
1182 243 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 1066 1656
Size: 379 687 590
Fragments arranged by size:
687 590 379
BsaI GGTCTCn'nnnn_
Cuts at: 0 469 781 1656
Size: 469 312 875
Fragments arranged by size:
875 469 312
BsaAI yAC'GTr
Cuts at: 0 654 1656
Size: 654 1002
BsaHI Gr'CG_yC
Cuts at: 0 374 1424 1656
Size: 374 1050 232
Fragments arranged by size:
1050 374 232
BsaWI w'CCGG_w
Cuts at: 0 85 1656
Size: 85 1571
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 614 644 1081 1111 1656
Size: 614 30 437 30 545
Fragments arranged by size:
614 545 437 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 134 433 1414 1656
Size: 134 299 981 242
Fragments arranged by size:
981 299 242 134
BsiHKAI G_wGCw'C
Cuts at: 0 350 659 1061 1580 1656
Size: 350 309 402 519 76
Fragments arranged by size:
519 402 350 309 76
Bsp1286I G_dGCh'C
Cuts at: 0 7 239 350 460 659 966 1005 1061
Size: 7 232 111 110 199 307 39 56
Cuts at: 1061 1163 1580 1628 1656
Size: 102 417 48 28
Fragments arranged by size:
417 307 232 199 111 110 102 56
48 39 28 7
BspEI T'CCGG_A
Cuts at: 0 85 1656
Size: 85 1571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 860 1605 1619 1656
Size: 860 745 14 37
Fragments arranged by size:
860 745 37 14
BsrFI r'CCGG_y
Cuts at: 0 947 1656
Size: 947 709
BssSI C'ACGA_G
Cuts at: 0 232 1656
Size: 232 1424
BstEII G'GTnAC_C
Cuts at: 0 151 1656
Size: 151 1505
BstYI r'GATC_y
Cuts at: 0 174 755 974 1656
Size: 174 581 219 682
Fragments arranged by size:
682 581 219 174
BstZ17I GTA'TAC
Cuts at: 0 444 1656
Size: 444 1212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 9 1656
Size: 9 1647
DraI TTT'AAA
Cuts at: 0 710 1074 1656
Size: 710 364 582
Fragments arranged by size:
710 582 364
DraIII CAC_nnn'GTG
Cuts at: 0 1441 1656
Size: 1441 215
EaeI y'GGCC_r
Cuts at: 0 389 642 1103 1208 1584 1656
Size: 389 253 461 105 376 72
Fragments arranged by size:
461 389 376 253 105 72
EarI CTCTTCn'nnn_
Cuts at: 0 1385 1490 1656
Size: 1385 105 166
Fragments arranged by size:
1385 166 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 379 1005 1066 1656
Size: 167 212 626 61 590
Fragments arranged by size:
626 590 212 167 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 748 1270 1656
Size: 748 522 386
Fragments arranged by size:
748 522 386
EcoO109I rG'GnC_Cy
Cuts at: 0 963 1347 1529 1624 1656
Size: 963 384 182 95 32
Fragments arranged by size:
963 384 182 95 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 552 1656
Size: 552 1104
HaeII r_GCGC'y
Cuts at: 0 1183 1656
Size: 1183 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1252 1284 1495 1498 1527 1530
Size: 1045 32 175 32 211 3 29 3
Cuts at: 1530 1621 1653 1656
Size: 91 32 3
Fragments arranged by size:
1045 211 175 91 32 32 32 29
3 3 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 1656
Size: 872 784
MscI TGG'CCA
Cuts at: 0 391 644 1105 1656
Size: 391 253 461 551
Fragments arranged by size:
551 461 391 253
MspA1I CmG'CkG
Cuts at: 0 1280 1656
Size: 1280 376
NaeI GCC'GGC
Cuts at: 0 949 1656
Size: 949 707
NgoMIV G'CCGG_C
Cuts at: 0 947 1656
Size: 947 709
PasI CC'CwG_GG
Cuts at: 0 1620 1656
Size: 1620 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 609 1044 1308 1441 1656
Size: 609 435 264 133 215
Fragments arranged by size:
609 435 264 215 133
PfoI T'CCnGG_A
Cuts at: 0 1307 1656
Size: 1307 349
PmlI CAC'GTG
Cuts at: 0 654 1656
Size: 654 1002
PpuMI rG'GwC_Cy
Cuts at: 0 1529 1656
Size: 1529 127
PspOMI G'GGCC_C
Cuts at: 0 962 1624 1656
Size: 962 662 32
Fragments arranged by size:
962 662 32
PspXI vC'TCGA_Gb
Cuts at: 0 1174 1656
Size: 1174 482
PstI C_TGCA'G
Cuts at: 0 752 974 1562 1656
Size: 752 222 588 94
Fragments arranged by size:
752 588 222 94
PvuII CAG'CTG
Cuts at: 0 1280 1656
Size: 1280 376
SanDI GG'GwC_CC
Cuts at: 0 1529 1656
Size: 1529 127
SbfI CC_TGCA'GG
Cuts at: 0 752 1562 1656
Size: 752 810 94
Fragments arranged by size:
810 752 94
SfcI C'TryA_G
Cuts at: 0 351 466 748 970 1558 1656
Size: 351 115 282 222 588 98
Fragments arranged by size:
588 351 282 222 115 98
SmaI CCC'GGG
Cuts at: 0 343 1656
Size: 343 1313
SmlI C'TyrA_G
Cuts at: 0 1174 1656
Size: 1174 482
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1429 1656
Size: 1429 227
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 614 646 1081 1113 1656
Size: 614 32 435 32 543
Fragments arranged by size:
614 543 435 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 733 1141 1656
Size: 733 408 515
Fragments arranged by size:
733 515 408
XhoI C'TCGA_G
Cuts at: 0 1174 1656
Size: 1174 482
XmaI C'CCGG_G
Cuts at: 0 341 1656
Size: 341 1315
Enzymes that do cut:
AarI AccI AcuI AhdI AlwNI ApaI ApoI AvaI
BanII BbsI BcgI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BsiHKAI Bsp1286I BspEI BspMI BsrFI BssSI BstEII BstYI
BstZ17I BtgZI DraI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I MmeI MscI MspA1I NaeI
NgoMIV PasI PflMI PfoI PmlI PpuMI PspOMI PspXI
PstI PvuII SanDI SbfI SfcI SmaI SmlI TaqII
TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BciVI BclI BfrBI BlpI
BmtI Bpu10I BpuEI BsaBI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BstAPI
BstBI BstXI Bsu36I BtgI BtsI ClaI CspCI DrdI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PshAI PsiI PsrI PvuI
RsrII SacI SacII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI XbaI XcmI XmnI ZraI