(Circular) (Six-base) MAPSORT of: pFN21ASDA0537.seq Check: 1355 from: 1 to: 6712 pFN21ASDA0537 6712 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4522 278 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3969 4055 1937 Size: 2032 86 4594 Fragments arranged by size: 4594 2032 86 Acc65I G'GTAC_C Cuts at: 3903 4033 3903 Size: 130 6582 AclI AA'CG_TT Cuts at: 1563 2654 4839 5212 1563 Size: 1091 2185 373 3063 Fragments arranged by size: 3063 2185 1091 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3146 3245 3917 4775 6159 6427 1632 Size: 1514 99 672 858 1384 268 1917 Fragments arranged by size: 1917 1514 1384 858 672 268 99 AfeI AGC'GCT Cuts at: 3981 3981 Size: 6712 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6515 AflIII A'CryG_T Cuts at: 1203 5611 6588 1203 Size: 4408 977 1327 Fragments arranged by size: 4408 1327 977 AgeI A'CCGG_T Cuts at: 5543 5543 Size: 6712 AhdI GACnn_n'nnGTC Cuts at: 2992 5441 2992 Size: 2449 4263 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6712 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6712 AlwNI CAG_nnn'CTG Cuts at: 3179 3683 3895 4455 6027 3179 Size: 504 212 560 1572 3864 Fragments arranged by size: 3864 1572 560 504 212 ApaI G_GGCC'C Cuts at: 2737 2737 Size: 6712 ApaLI G'TGCA_C Cuts at: 2519 4769 5925 2519 Size: 2250 1156 3306 Fragments arranged by size: 3306 2250 1156 ApoI r'AATT_y Cuts at: 1493 4021 4247 4399 6508 1493 Size: 2528 226 152 2109 1697 Fragments arranged by size: 2528 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5266 160 Size: 872 4234 1606 Fragments arranged by size: 4234 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 AvaI C'yCGr_G Cuts at: 1870 1943 2091 2859 4037 1870 Size: 73 148 768 1178 4545 Fragments arranged by size: 4545 1178 768 148 73 BamHI G'GATC_C Cuts at: 3219 4042 4409 3219 Size: 823 367 5522 Fragments arranged by size: 5522 823 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 2213 3024 3370 3623 Size: 325 217 627 290 136 811 346 253 Cuts at: 3623 3903 4033 5488 618 Size: 280 130 1455 1842 Fragments arranged by size: 1842 1455 811 627 346 325 290 280 253 217 136 130 BanII G_rGCy'C Cuts at: 1391 2087 2130 2737 3870 4031 1391 Size: 696 43 607 1133 161 4072 Fragments arranged by size: 4072 1133 696 607 161 43 BbeI G_GCGC'C Cuts at: 2081 2081 Size: 6712 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3474 928 Size: 971 1575 4166 Fragments arranged by size: 4166 1575 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3776 3810 4903 4937 1903 Size: 34 1839 34 1093 34 3678 Fragments arranged by size: 3678 1839 1093 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4604 5814 4604 Size: 1210 5502 BclI T'GATC_A Cuts at: 1548 2409 1548 Size: 861 5851 BfrBI ATG'CAT Cuts at: 2678 2678 Size: 6712 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5323 136 Size: 107 122 71 631 605 3651 1525 Fragments arranged by size: 3651 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3987 6707 3987 Size: 2720 3992 BlpI GC'TnA_GC Cuts at: 4129 4129 Size: 6712 Bme1580I G_kGCm'C Cuts at: 1790 2523 2737 3626 4773 5929 1790 Size: 733 214 889 1147 1156 2573 Fragments arranged by size: 2573 1156 1147 889 733 214 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6712 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2953 3883 4443 Size: 416 53 83 399 24 1536 930 560 Cuts at: 4443 5401 442 Size: 958 1753 Fragments arranged by size: 1753 1536 958 930 560 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6712 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3268 3721 3268 Size: 453 6259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 3830 3854 4004 5372 1239 Size: 905 1686 24 150 1368 2579 Fragments arranged by size: 2579 1686 1368 905 150 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4837 5702 6000 4837 Size: 865 298 5549 Fragments arranged by size: 5549 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2192 2477 2695 5375 882 Size: 617 693 285 218 2680 2219 Fragments arranged by size: 2680 2219 693 617 285 218 BsaAI yAC'GTr Cuts at: 493 1204 4009 4503 493 Size: 711 2805 494 2702 Fragments arranged by size: 2805 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2336 2408 6706 2336 Size: 72 4298 2342 Fragments arranged by size: 4298 2342 72 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 2467 4519 4901 Size: 53 83 186 936 545 389 2052 382 Cuts at: 4901 5536 275 Size: 635 1451 Fragments arranged by size: 2052 1451 936 635 545 389 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3906 5145 5543 5817 5964 6375 3906 Size: 1239 398 274 147 411 4243 Fragments arranged by size: 4243 1239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3557 3587 1178 Size: 30 165 30 2154 30 4303 Fragments arranged by size: 4303 2154 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3335 3929 1186 Size: 2149 594 3969 Fragments arranged by size: 3969 2149 594 BseYI C'CCAG_C Cuts at: 2071 2730 3435 3533 3547 3634 5915 6411 2071 Size: 659 705 98 14 87 2281 496 2372 Fragments arranged by size: 2372 2281 705 659 496 98 87 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3349 1163 Size: 573 1613 4526 Fragments arranged by size: 4526 1613 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2899 3122 4162 4923 5951 1540 Size: 275 186 898 223 1040 761 1028 2301 Fragments arranged by size: 2301 1040 1028 898 761 275 223 186 BsiHKAI G_wGCw'C Cuts at: 2523 3345 4773 4858 5929 2523 Size: 822 1428 85 1071 3306 Fragments arranged by size: 3306 1428 1071 822 85 BsmI GAATG_Cn' Cuts at: 4223 4316 4223 Size: 93 6619 BsmBI CGTCTCn'nnnn_ Cuts at: 2515 3832 2515 Size: 1317 5395 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2130 2523 2737 3345 3626 3870 Size: 399 297 43 393 214 608 281 244 Cuts at: 3870 4031 4773 4858 5929 1391 Size: 161 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 608 399 393 297 281 244 214 161 85 43 BspEI T'CCGG_A Cuts at: 6375 6375 Size: 6712 BspHI T'CATG_A Cuts at: 4601 6331 4601 Size: 1730 4982 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4067 844 Size: 3223 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2461 3112 4599 1394 Size: 429 638 651 1487 3507 Fragments arranged by size: 3507 1487 651 638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2919 5207 5381 65 Size: 1183 1671 2288 174 1396 Fragments arranged by size: 2288 1671 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3876 3912 5356 5543 1700 Size: 110 2066 36 1444 187 2869 Fragments arranged by size: 2869 2066 1444 187 110 36 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6712 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6712 BssSI C'ACGA_G Cuts at: 4772 5784 4772 Size: 1012 5700 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6712 BstBI TT'CG_AA Cuts at: 2007 5516 2007 Size: 3509 3203 BstEII G'GTnAC_C Cuts at: 1184 3556 1184 Size: 2372 4340 BstYI r'GATC_y Cuts at: 1972 3219 3849 3987 4042 4409 4794 4811 6263 Size: 1247 630 138 55 367 385 17 1452 Cuts at: 6263 6349 6361 6707 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1247 630 385 367 346 138 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3627 513 Size: 552 957 1605 3598 Fragments arranged by size: 3598 1605 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3233 3756 487 Size: 683 56 2007 523 3443 Fragments arranged by size: 3443 2007 683 523 56 BtsI GCAGTG_nn' Cuts at: 3180 3481 4228 5022 5042 3180 Size: 301 747 794 20 4850 Fragments arranged by size: 4850 794 747 301 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6677 DraI TTT'AAA Cuts at: 4016 4016 Size: 6712 DraIII CAC_nnn'GTG Cuts at: 2146 2146 Size: 6712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5719 809 Size: 507 4403 1802 Fragments arranged by size: 4403 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2254 3078 3399 Size: 54 1425 50 275 18 424 824 321 Cuts at: 3399 4159 5048 6677 8 Size: 760 889 1629 43 Fragments arranged by size: 1629 1425 889 824 760 424 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4159 1537 Size: 275 2347 4090 Fragments arranged by size: 4090 2347 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3466 4642 1353 Size: 2113 1176 3423 Fragments arranged by size: 3423 2113 1176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5299 5673 5819 387 Size: 1064 138 3710 374 146 1280 Fragments arranged by size: 3710 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 3146 3245 3830 3854 3917 4004 Size: 393 512 1002 99 585 24 63 87 Cuts at: 4004 4775 5372 6159 6427 1239 Size: 771 597 787 268 1524 Fragments arranged by size: 1524 1002 787 771 597 585 512 393 268 99 87 63 24 EcoNI CCTnn'n_nnAGG Cuts at: 2709 3580 2709 Size: 871 5841 EcoO109I rG'GnC_Cy Cuts at: 2734 3073 3700 2734 Size: 339 627 5746 Fragments arranged by size: 5746 627 339 EcoRI G'AATT_C Cuts at: 4021 4399 4021 Size: 378 6334 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 3610 2188 Size: 1422 5290 FspI TGC'GCA Cuts at: 5218 5218 Size: 6712 HaeII r_GCGC'y Cuts at: 1678 2081 3983 5859 1678 Size: 403 1902 1876 2531 Fragments arranged by size: 2531 1902 1876 403 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2327 2359 2978 3010 5353 Size: 32 135 32 755 32 619 32 2343 Cuts at: 5353 5385 5427 5459 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2343 755 619 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4056 4302 669 Size: 988 281 2118 246 3079 Fragments arranged by size: 3079 2118 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6712 HpaI GTT'AAC Cuts at: 4302 4302 Size: 6712 KasI G'GCGC_C Cuts at: 2077 2077 Size: 6712 KpnI G_GTAC'C Cuts at: 3907 4037 3907 Size: 130 6582 MfeI C'AATT_G Cuts at: 4311 4311 Size: 6712 MluI A'CGCG_T Cuts at: 6588 6588 Size: 6712 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2646 3474 5826 6010 1305 Size: 1341 828 2352 184 2007 Fragments arranged by size: 2352 2007 1341 828 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3080 10 Size: 54 1425 343 1248 3642 Fragments arranged by size: 3642 1425 1248 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3947 4670 5029 5188 518 Size: 648 2781 723 359 159 2042 Fragments arranged by size: 2781 2042 723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2730 2823 3118 3620 3822 4128 4805 5953 Size: 1124 93 295 502 202 306 677 1148 Cuts at: 5953 6198 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1124 677 502 306 295 245 202 93 NarI GG'CG_CC Cuts at: 2078 2078 Size: 6712 NcoI C'CATG_G Cuts at: 513 1065 2022 3627 513 Size: 552 957 1605 3598 Fragments arranged by size: 3598 1605 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6712 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6712 NotI GC'GGCC_GC Cuts at: 4159 4159 Size: 6712 NsiI A_TGCA'T Cuts at: 2680 2680 Size: 6712 NspI r_CATG'y Cuts at: 1132 1229 4070 5615 1132 Size: 97 2841 1545 2229 Fragments arranged by size: 2841 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2203 2737 1348 Size: 855 534 5323 Fragments arranged by size: 5323 855 534 PciI A'CATG_T Cuts at: 5611 5611 Size: 6712 PflMI CCAn_nnn'nTGG Cuts at: 1108 2931 1108 Size: 1823 4889 PfoI T'CCnGG_A Cuts at: 3707 3852 3707 Size: 145 6567 PmeI GTTT'AAAC Cuts at: 4016 4016 Size: 6712 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4781 4813 6294 6326 4781 Size: 32 1481 32 5167 Fragments arranged by size: 5167 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3965 1613 Size: 2352 4360 PsiI TTA'TAA Cuts at: 4282 4282 Size: 6712 PspOMI G'GGCC_C Cuts at: 2733 2733 Size: 6712 PspXI vC'TCGA_Gb Cuts at: 2859 2859 Size: 6712 PstI C_TGCA'G Cuts at: 830 2119 2196 3048 3835 3995 4064 830 Size: 1289 77 852 787 160 69 3478 Fragments arranged by size: 3478 1289 852 787 160 77 69 PvuI CG_AT'CG Cuts at: 2001 2899 2001 Size: 898 5814 PvuII CAG'CTG Cuts at: 2730 3822 2730 Size: 1092 5620 SalI G'TCGA_C Cuts at: 1936 4054 1936 Size: 2118 4594 SbfI CC_TGCA'GG Cuts at: 4064 4064 Size: 6712 ScaI AGT'ACT Cuts at: 4960 6672 4960 Size: 1712 5000 SfcI C'TryA_G Cuts at: 826 1045 2115 2192 3044 3715 3831 3991 4060 Size: 219 1070 77 852 671 116 160 69 Cuts at: 4060 5195 5876 6067 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1070 852 681 671 219 191 160 116 77 69 SfoI GGC'GCC Cuts at: 2079 2079 Size: 6712 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6712 SmaI CCC'GGG Cuts at: 1872 4039 1872 Size: 2167 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2859 4816 5717 5979 820 Size: 197 926 916 1957 901 262 1553 Fragments arranged by size: 1957 1553 926 916 901 262 197 SnaBI TAC'GTA Cuts at: 493 4009 4503 493 Size: 3516 494 2702 Fragments arranged by size: 3516 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6712 SphI G_CATG'C Cuts at: 1132 4070 1132 Size: 2938 3774 SspI AAT'ATT Cuts at: 5 52 2596 4636 5 Size: 47 2544 2040 2081 Fragments arranged by size: 2544 2081 2040 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6712 StyI C'CwwG_G Cuts at: 513 1065 2022 3213 3627 4151 513 Size: 552 957 1191 414 524 3074 Fragments arranged by size: 3074 1191 957 552 524 414 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2729 2926 4739 4756 4909 5094 5539 Size: 1255 1149 197 1813 17 153 185 445 Cuts at: 5539 325 Size: 1498 Fragments arranged by size: 1813 1498 1255 1149 445 197 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4958 6670 96 Size: 274 80 33 51 1443 2981 1712 138 Fragments arranged by size: 2981 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3017 3049 3557 3589 6672 6704 3017 Size: 32 508 32 3083 32 3025 Fragments arranged by size: 3083 3025 508 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6712 XbaI T'CTAG_A Cuts at: 4048 4048 Size: 6712 XhoI C'TCGA_G Cuts at: 1943 2859 1943 Size: 916 5796 XmaI C'CCGG_G Cuts at: 1870 4037 1870 Size: 2167 4545 XmnI GAAnn'nnTTC Cuts at: 4841 4841 Size: 6712 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4520 276 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI