Restriction map for pFN21AB8804
 (Circular) (Six-base) MAPSORT of: pFN21AB8804.seq  Check: 9901  from: 1  to: 5755



pFN21AB8804  5755 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:50  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3565     278 
   Size:         53      83     186     936    2029    2468

  Fragments arranged by size: 

                2468    2029     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3098    1937 
   Size:       1161    4594
 
Acc65I G'GTAC_C

Cuts at:   3076    3076 
   Size:       5755
 
AclI AA'CG_TT

Cuts at:   1563    3882    4255    1563 
   Size:       2319     373    3063

  Fragments arranged by size: 

                3063    2319     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2334    3818    5202    5470    1632 
   Size:        702    1484    1384     268    1917

  Fragments arranged by size: 

                1917    1484    1384     702     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5558
 
AflIII A'CryG_T

Cuts at:   1203    4654    5631    1203 
   Size:       3451     977    1327

  Fragments arranged by size: 

                3451    1327     977
 
AgeI A'CCGG_T

Cuts at:   4586    4586 
   Size:       5755
 
AhdI GACnn_n'nnGTC

Cuts at:   4484    4484 
   Size:       5755
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5755
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5755
 
AlwNI CAG_nnn'CTG

Cuts at:   2392    3498    5070    2392 
   Size:       1106    1572    3077

  Fragments arranged by size: 

                3077    1572    1106
 
ApaLI G'TGCA_C

Cuts at:   3812    4968    3812 
   Size:       1156    4599
 
ApoI r'AATT_y

Cuts at:   1493    2117    2248    3064    3290    3442    5551    1493 
   Size:        624     131     816     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     816     624     226     152     131
 
AseI AT'TA_AT

Cuts at:    160    1032    4309     160 
   Size:        872    3277    1606

  Fragments arranged by size: 

                3277    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5755
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3080    1870 
   Size:         73    1137    4545

  Fragments arranged by size: 

                4545    1137      73
 
AvrII C'CTAG_G

Cuts at:   2578    2578 
   Size:       5755
 
BamHI G'GATC_C

Cuts at:   3085    3452    3085 
   Size:        367    5388
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3076    4531     618 
   Size:        325     217     627    1289    1455    1842

  Fragments arranged by size: 

                1842    1455    1289     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3074    1391 
   Size:       1683    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2986     928 
   Size:        971    1087    3697

  Fragments arranged by size: 

                3697    1087     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2833    2867    3946    3980    1903 
   Size:         34     896      34    1079      34    3678

  Fragments arranged by size: 

                3678    1079     896      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3647    4857    3647 
   Size:       1210    4545
 
BclI T'GATC_A

Cuts at:   1548    2817    1548 
   Size:       1269    4486
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4366     136 
   Size:        107     122      71     631     605    2694    1525

  Fragments arranged by size: 

                2694    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2616    5750    2616 
   Size:       3134    2621
 
BlpI GC'TnA_GC

Cuts at:   2507    3172    2507 
   Size:        665    5090
 
Bme1580I G_kGCm'C

Cuts at:   1790    3816    4972    1790 
   Size:       2026    1156    2573

  Fragments arranged by size: 

                2573    2026    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5755
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3486    4444     442 
   Size:        416      53      83     399      24    2069     958    1753

  Fragments arranged by size: 

                2069    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5755
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2995    4415    1239 
   Size:       1756    1420    2579

  Fragments arranged by size: 

                2579    1756    1420
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3880    4745    5043    3880 
   Size:        865     298    4592

  Fragments arranged by size: 

                4592     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4418     882 
   Size:        617    2919    2219

  Fragments arranged by size: 

                2919    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3546     493 
   Size:        711    2342    2702

  Fragments arranged by size: 

                2702    2342     711
 
BsaBI GATnn'nnATC

Cuts at:   2096    5749    2096 
   Size:       3653    2102
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3562    3944    4579     275 
   Size:         53      83     186     936    2029     382     635    1451

  Fragments arranged by size: 

                2029    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4188    4586    4860    5007    5418    4188 
   Size:        398     274     147     411    4525

  Fragments arranged by size: 

                4525     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5530

  Fragments arranged by size: 

                5530     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3014    1186 
   Size:       1828    3927
 
BseYI C'CCAG_C

Cuts at:   2438    2517    4958    5454    2438 
   Size:         79    2441     496    2739

  Fragments arranged by size: 

                2739    2441     496      79
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5182
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3205    3966    4994    1540 
   Size:        275     186    1204     761    1028    2301

  Fragments arranged by size: 

                2301    1204    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3816    3901    4972    3816 
   Size:         85    1071    4599

  Fragments arranged by size: 

                4599    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2388    2556    3266    3359    2388 
   Size:        168     710      93    4784

  Fragments arranged by size: 

                4784     710     168      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3074    3816    3901    4972    1391 
   Size:        399    1284     742      85    1071    2174

  Fragments arranged by size: 

                2174    1284    1071     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5418    5418 
   Size:       5755
 
BspHI T'CATG_A

Cuts at:   2685    3644    5374    2685 
   Size:        959    1730    3066

  Fragments arranged by size: 

                3066    1730     959
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3110     844 
   Size:       2266    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3642    1394 
   Size:        429    1819    3507

  Fragments arranged by size: 

                3507    1819     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4250    4424      65 
   Size:       1183    3002     174    1396

  Fragments arranged by size: 

                3002    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4399    4586    1700 
   Size:        110    2589     187    2869

  Fragments arranged by size: 

                2869    2589     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2238    2358      96 
   Size:       2142     120    3493

  Fragments arranged by size: 

                3493    2142     120
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5755
 
BssSI C'ACGA_G

Cuts at:   3815    4827    3815 
   Size:       1012    4743
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2201     794 
   Size:       1407    4348
 
BstBI TT'CG_AA

Cuts at:   4559    4559 
   Size:       5755
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5755
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2011    2191    2011 
   Size:        180    5575
 
BstYI r'GATC_y

Cuts at:   1972    2616    3085    3452    3837    3854    5306    5392    5404 
   Size:        644     469     367     385      17    1452      86      12

Cuts at:   5404    5750    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     644     469     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2184     513 
   Size:        552     939     180    4084

  Fragments arranged by size: 

                4084     939     552     180
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2836    2860     487 
   Size:        683      56    1610      24    3382

  Fragments arranged by size: 

                3382    1610     683      56      24
 
BtsI GCAGTG_nn'

Cuts at:   3271    4065    4085    3271 
   Size:        794      20    4941

  Fragments arranged by size: 

                4941     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5755
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5720
 
DraI TTT'AAA

Cuts at:   3059    3059 
   Size:       5755
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4762     809 
   Size:        507    3446    1802

  Fragments arranged by size: 

                3446    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2955    3202    4091 
   Size:         54    1425      50     275      18    1125     247     889

Cuts at:   4091    5720       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1125     889     275     247      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3202    1537 
   Size:        275    1390    4090

  Fragments arranged by size: 

                4090    1390     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3685    1353 
   Size:       2332    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4342    4716    4862     387 
   Size:       1064     138    2753     374     146    1280

  Fragments arranged by size: 

                2753    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2334    2995    3818    4415    5202    5470    1239 
   Size:        393     702     661     823     597     787     268    1524

  Fragments arranged by size: 

                1524     823     787     702     661     597     393     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2970    3023    2970 
   Size:         53    5702
 
EcoRI G'AATT_C

Cuts at:   3064    3442    3064 
   Size:        378    5377
 
FspI TGC'GCA

Cuts at:   4261    4261 
   Size:       5755
 
HaeII r_GCGC'y

Cuts at:   1678    4902    1678 
   Size:       3224    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4396    4428    4470    4502    1373 
   Size:         32     135      32    2824      32      42      32    2626

  Fragments arranged by size: 

                2824    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2028    3099    3345     669 
   Size:        988     281      90    1071     246    3079

  Fragments arranged by size: 

                3079    1071     988     281     246      90
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5755
 
HpaI GTT'AAC

Cuts at:   3345    3345 
   Size:       5755
 
KpnI G_GTAC'C

Cuts at:   3080    3080 
   Size:       5755
 
MfeI C'AATT_G

Cuts at:   3354    3354 
   Size:       5755
 
MluI A'CGCG_T

Cuts at:   5631    5631 
   Size:       5755
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4869    5053    1305 
   Size:       3564     184    2007

  Fragments arranged by size: 

                3564    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2957      10 
   Size:         54    1425     343    1125    2808

  Fragments arranged by size: 

                2808    1425    1125     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2189    2690    3713    4072    4231     518 
   Size:        648    1023     501    1023     359     159    2042

  Fragments arranged by size: 

                2042    1023    1023     648     501     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2521    2826    2892    3171    3848    4996    5241    1606 
   Size:        915     305      66     279     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     915     677     305     279     245      66
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2184     513 
   Size:        552     939     180    4084

  Fragments arranged by size: 

                4084     939     552     180
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5755
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5755
 
NotI GC'GGCC_GC

Cuts at:   3202    3202 
   Size:       5755
 
NspI r_CATG'y

Cuts at:   1132    1229    3113    4658    1132 
   Size:         97    1884    1545    2229

  Fragments arranged by size: 

                2229    1884    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5755
 
PciI A'CATG_T

Cuts at:   4654    4654 
   Size:       5755
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5755
 
PfoI T'CCnGG_A

Cuts at:   2972    2972 
   Size:       5755
 
PmeI GTTT'AAAC

Cuts at:   3059    3059 
   Size:       5755
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3824    3856    5337    5369    3824 
   Size:         32    1481      32    4210

  Fragments arranged by size: 

                4210    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2970    3023    2970 
   Size:         53    5702
 
PshAI GACnn'nnGTC

Cuts at:   1613    2076    1613 
   Size:        463    5292
 
PsiI TTA'TAA

Cuts at:   2772    3325    2772 
   Size:        553    5202
 
PstI C_TGCA'G

Cuts at:    830    3107     830 
   Size:       2277    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5755
 
PvuII CAG'CTG

Cuts at:   2521    2826    2892    2521 
   Size:        305      66    5384

  Fragments arranged by size: 

                5384     305      66
 
SalI G'TCGA_C

Cuts at:   1936    3097    1936 
   Size:       1161    4594
 
SbfI CC_TGCA'GG

Cuts at:   3107    3107 
   Size:       5755
 
ScaI AGT'ACT

Cuts at:   3043    4003    5715    3043 
   Size:        960    1712    3083

  Fragments arranged by size: 

                3083    1712     960
 
SfcI C'TryA_G

Cuts at:    826    1045    2074    2256    3027    3103    4238    4919    5110 
   Size:        219    1029     182     771      76    1135     681     191

Cuts at:   5110     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135    1029     771     681     219     191     182
                  76
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5755
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5755
 
SmaI CCC'GGG

Cuts at:   1872    3082    1872 
   Size:       1210    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3859    4760    5022     820 
   Size:        197     926    1916     901     262    1553

  Fragments arranged by size: 

                1916    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3546     493 
   Size:       3053    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5755
 
SphI G_CATG'C

Cuts at:   1132    3113    1132 
   Size:       1981    3774
 
SspI AAT'ATT

Cuts at:      5      52    3679       5 
   Size:         47    3627    2081

  Fragments arranged by size: 

                3627    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5755
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2184    2578    3194     513 
   Size:        552     939     180     394     616    3074

  Fragments arranged by size: 

                3074     939     616     552     394     180
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2304    3782    3799    3952    4137    4582     325 
   Size:       1255     724    1478      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1478    1255     724     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2238    2358    3041 
   Size:        274      80      33      51    1443     261     120     683

Cuts at:   3041    4001    5713      96 
   Size:        960    1712     138

  Fragments arranged by size: 

                1712    1443     960     683     274     261     138     120
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5715    5747    5715 
   Size:         32    5723
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5755
 
XbaI T'CTAG_A

Cuts at:   3091    3091 
   Size:       5755
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5755
 
XmaI C'CCGG_G

Cuts at:   1870    3080    1870 
   Size:       1210    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3884    3884 
   Size:       5755
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3563     276 
   Size:         53      83     186     936    2029    2468

  Fragments arranged by size: 

                2468    2029     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PmlI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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