(Circular) (Six-base) MAPSORT of: pFN21AB8804.seq Check: 9901 from: 1 to: 5755 pFN21AB8804 5755 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2334 3818 5202 5470 1632 Size: 702 1484 1384 268 1917 Fragments arranged by size: 1917 1484 1384 702 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AlwNI CAG_nnn'CTG Cuts at: 2392 3498 5070 2392 Size: 1106 1572 3077 Fragments arranged by size: 3077 1572 1106 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 2117 2248 3064 3290 3442 5551 1493 Size: 624 131 816 226 152 2109 1697 Fragments arranged by size: 2109 1697 816 624 226 152 131 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 3080 1870 Size: 73 1137 4545 Fragments arranged by size: 4545 1137 73 AvrII C'CTAG_G Cuts at: 2578 2578 Size: 5755 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3076 4531 618 Size: 325 217 627 1289 1455 1842 Fragments arranged by size: 1842 1455 1289 627 325 217 BanII G_rGCy'C Cuts at: 1391 3074 1391 Size: 1683 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2986 928 Size: 971 1087 3697 Fragments arranged by size: 3697 1087 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2833 2867 3946 3980 1903 Size: 34 896 34 1079 34 3678 Fragments arranged by size: 3678 1079 896 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 2817 1548 Size: 1269 4486 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2616 5750 2616 Size: 3134 2621 BlpI GC'TnA_GC Cuts at: 2507 3172 2507 Size: 665 5090 Bme1580I G_kGCm'C Cuts at: 1790 3816 4972 1790 Size: 2026 1156 2573 Fragments arranged by size: 2573 2026 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2995 4415 1239 Size: 1756 1420 2579 Fragments arranged by size: 2579 1756 1420 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3880 4745 5043 3880 Size: 865 298 4592 Fragments arranged by size: 4592 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 2096 5749 2096 Size: 3653 2102 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3562 3944 4579 275 Size: 53 83 186 936 2029 382 635 1451 Fragments arranged by size: 2029 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5530 Fragments arranged by size: 5530 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3014 1186 Size: 1828 3927 BseYI C'CCAG_C Cuts at: 2438 2517 4958 5454 2438 Size: 79 2441 496 2739 Fragments arranged by size: 2739 2441 496 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3816 3901 4972 3816 Size: 85 1071 4599 Fragments arranged by size: 4599 1071 85 BsmI GAATG_Cn' Cuts at: 2388 2556 3266 3359 2388 Size: 168 710 93 4784 Fragments arranged by size: 4784 710 168 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3074 3816 3901 4972 1391 Size: 399 1284 742 85 1071 2174 Fragments arranged by size: 2174 1284 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 2685 3644 5374 2685 Size: 959 1730 3066 Fragments arranged by size: 3066 1730 959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3110 844 Size: 2266 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4399 4586 1700 Size: 110 2589 187 2869 Fragments arranged by size: 2869 2589 187 110 BsrGI T'GTAC_A Cuts at: 96 2238 2358 96 Size: 2142 120 3493 Fragments arranged by size: 3493 2142 120 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 2201 794 Size: 1407 4348 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2191 2011 Size: 180 5575 BstYI r'GATC_y Cuts at: 1972 2616 3085 3452 3837 3854 5306 5392 5404 Size: 644 469 367 385 17 1452 86 12 Cuts at: 5404 5750 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 644 469 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2184 513 Size: 552 939 180 4084 Fragments arranged by size: 4084 939 552 180 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2836 2860 487 Size: 683 56 1610 24 3382 Fragments arranged by size: 3382 1610 683 56 24 BtsI GCAGTG_nn' Cuts at: 3271 4065 4085 3271 Size: 794 20 4941 Fragments arranged by size: 4941 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2955 3202 4091 Size: 54 1425 50 275 18 1125 247 889 Cuts at: 4091 5720 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1125 889 275 247 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3685 1353 Size: 2332 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 2995 3818 4415 5202 5470 1239 Size: 393 702 661 823 597 787 268 1524 Fragments arranged by size: 1524 823 787 702 661 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2970 3023 2970 Size: 53 5702 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 4902 1678 Size: 3224 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4396 4428 4470 4502 1373 Size: 32 135 32 2824 32 42 32 2626 Fragments arranged by size: 2824 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2028 3099 3345 669 Size: 988 281 90 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 90 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4869 5053 1305 Size: 3564 184 2007 Fragments arranged by size: 3564 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2957 10 Size: 54 1425 343 1125 2808 Fragments arranged by size: 2808 1425 1125 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2690 3713 4072 4231 518 Size: 648 1023 501 1023 359 159 2042 Fragments arranged by size: 2042 1023 1023 648 501 359 159 MspA1I CmG'CkG Cuts at: 1606 2521 2826 2892 3171 3848 4996 5241 1606 Size: 915 305 66 279 677 1148 245 2120 Fragments arranged by size: 2120 1148 915 677 305 279 245 66 NcoI C'CATG_G Cuts at: 513 1065 2004 2184 513 Size: 552 939 180 4084 Fragments arranged by size: 4084 939 552 180 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 3113 4658 1132 Size: 97 1884 1545 2229 Fragments arranged by size: 2229 1884 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5755 PfoI T'CCnGG_A Cuts at: 2972 2972 Size: 5755 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2970 3023 2970 Size: 53 5702 PshAI GACnn'nnGTC Cuts at: 1613 2076 1613 Size: 463 5292 PsiI TTA'TAA Cuts at: 2772 3325 2772 Size: 553 5202 PstI C_TGCA'G Cuts at: 830 3107 830 Size: 2277 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 PvuII CAG'CTG Cuts at: 2521 2826 2892 2521 Size: 305 66 5384 Fragments arranged by size: 5384 305 66 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 3043 4003 5715 3043 Size: 960 1712 3083 Fragments arranged by size: 3083 1712 960 SfcI C'TryA_G Cuts at: 826 1045 2074 2256 3027 3103 4238 4919 5110 Size: 219 1029 182 771 76 1135 681 191 Cuts at: 5110 826 Size: 1471 Fragments arranged by size: 1471 1135 1029 771 681 219 191 182 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3859 4760 5022 820 Size: 197 926 1916 901 262 1553 Fragments arranged by size: 1916 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2004 2184 2578 3194 513 Size: 552 939 180 394 616 3074 Fragments arranged by size: 3074 939 616 552 394 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2304 3782 3799 3952 4137 4582 325 Size: 1255 724 1478 17 153 185 445 1498 Fragments arranged by size: 1498 1478 1255 724 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2238 2358 3041 Size: 274 80 33 51 1443 261 120 683 Cuts at: 3041 4001 5713 96 Size: 960 1712 138 Fragments arranged by size: 1712 1443 960 683 274 261 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5715 5747 5715 Size: 32 5723 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI