(Circular) (Six-base) MAPSORT of: pFN21ASDA0422.seq Check: 6934 from: 1 to: 8074 pFN21ASDA0422 8074 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2117 2383 3021 4597 5135 2117 Size: 266 638 1576 538 5056 Fragments arranged by size: 5056 1576 638 538 266 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5884 278 Size: 53 83 186 936 4348 2468 Fragments arranged by size: 4348 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2760 3378 4616 5417 1937 Size: 823 618 1238 801 4594 Fragments arranged by size: 4594 1238 823 801 618 Acc65I G'GTAC_C Cuts at: 5395 5395 Size: 8074 AclI AA'CG_TT Cuts at: 1563 6201 6574 1563 Size: 4638 373 3063 Fragments arranged by size: 4638 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 6137 7521 7789 1632 Size: 4505 1384 268 1917 Fragments arranged by size: 4505 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7877 AflIII A'CryG_T Cuts at: 1203 3639 4302 6973 7950 1203 Size: 2436 663 2671 977 1327 Fragments arranged by size: 2671 2436 1327 977 663 AgeI A'CCGG_T Cuts at: 6905 6905 Size: 8074 AhdI GACnn_n'nnGTC Cuts at: 6803 6803 Size: 8074 AleI CACnn'nnGTG Cuts at: 1166 2513 3360 1166 Size: 1347 847 5880 Fragments arranged by size: 5880 1347 847 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2907 3220 4154 1184 Size: 1723 313 934 5104 Fragments arranged by size: 5104 1723 934 313 AlwNI CAG_nnn'CTG Cuts at: 3220 3592 5817 7389 3220 Size: 372 2225 1572 3905 Fragments arranged by size: 3905 2225 1572 372 ApaI G_GGCC'C Cuts at: 2201 5135 5360 2201 Size: 2934 225 4915 Fragments arranged by size: 4915 2934 225 ApaLI G'TGCA_C Cuts at: 3205 3461 6131 7287 3205 Size: 256 2670 1156 3992 Fragments arranged by size: 3992 2670 1156 256 ApoI r'AATT_y Cuts at: 1493 5383 5609 5761 7870 1493 Size: 3890 226 152 2109 1697 Fragments arranged by size: 3890 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6628 160 Size: 872 5596 1606 Fragments arranged by size: 5596 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8074 AvaI C'yCGr_G Cuts at: 1870 1943 2121 3339 3573 3738 4749 5399 1870 Size: 73 178 1218 234 165 1011 650 4545 Fragments arranged by size: 4545 1218 1011 650 234 178 165 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2292 2325 3572 3605 2292 Size: 33 1247 33 6761 Fragments arranged by size: 6761 1247 33 33 BamHI G'GATC_C Cuts at: 4018 5404 5771 4018 Size: 1386 367 6321 Fragments arranged by size: 6321 1386 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2109 2208 2341 2353 2379 Size: 325 217 627 322 99 133 12 26 Cuts at: 2379 2742 2891 3683 3860 5395 6850 618 Size: 363 149 792 177 1535 1455 1842 Fragments arranged by size: 1842 1535 1455 792 627 363 325 322 217 177 149 133 99 26 12 BanII G_rGCy'C Cuts at: 1391 2201 2604 3243 4404 5135 5161 5360 5393 Size: 810 403 639 1161 731 26 199 33 Cuts at: 5393 1391 Size: 4072 Fragments arranged by size: 4072 1161 810 731 639 403 199 33 26 BbeI G_GCGC'C Cuts at: 2113 2345 2383 3687 2113 Size: 232 38 1304 6500 Fragments arranged by size: 6500 1304 232 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3096 3117 4051 4342 928 Size: 971 1197 21 934 291 4660 Fragments arranged by size: 4660 1197 971 934 291 21 BbvCI CC'TCA_GC Cuts at: 2334 2550 2807 2334 Size: 216 257 7601 Fragments arranged by size: 7601 257 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3008 3042 3719 3753 6265 6299 1903 Size: 34 1071 34 677 34 2512 34 3678 Fragments arranged by size: 3678 2512 1071 677 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2293 5966 7176 2293 Size: 3673 1210 3191 Fragments arranged by size: 3673 3191 1210 BclI T'GATC_A Cuts at: 1548 3934 1548 Size: 2386 5688 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2564 3555 3627 Size: 107 122 71 631 605 892 991 72 Cuts at: 3627 3719 3828 6685 136 Size: 92 109 2857 1525 Fragments arranged by size: 2857 1525 991 892 631 605 122 109 107 92 72 71 BglII A'GATC_T Cuts at: 3129 3294 8069 3129 Size: 165 4775 3134 Fragments arranged by size: 4775 3134 165 BlpI GC'TnA_GC Cuts at: 4602 5491 4602 Size: 889 7185 Bme1580I G_kGCm'C Cuts at: 1790 2201 2213 2358 2747 3209 3465 3863 4051 Size: 411 12 145 389 462 256 398 188 Cuts at: 4051 4243 5135 5360 6135 7291 1790 Size: 192 892 225 775 1156 2573 Fragments arranged by size: 2573 1156 892 775 462 411 398 389 256 225 192 188 145 12 BmgBI CAC'GTC Cuts at: 1318 4729 1318 Size: 3411 4663 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3196 5129 5805 Size: 416 53 83 399 24 1779 1933 676 Cuts at: 5805 6763 442 Size: 958 1753 Fragments arranged by size: 1933 1779 1753 958 676 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8074 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3412 3412 Size: 8074 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 4868 5312 6734 1239 Size: 1211 2418 444 1422 2579 Fragments arranged by size: 2579 2418 1422 1211 444 Bpu10I CC'TnA_GC Cuts at: 2334 2550 2807 3905 2334 Size: 216 257 1098 6503 Fragments arranged by size: 6503 1098 257 216 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3632 4013 6199 7064 7362 3632 Size: 381 2186 865 298 4344 Fragments arranged by size: 4344 2186 865 381 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2975 4493 6737 882 Size: 617 1476 1518 2244 2219 Fragments arranged by size: 2244 2219 1518 1476 617 BsaAI yAC'GTr Cuts at: 493 1204 2662 3407 5371 5865 493 Size: 711 1458 745 1964 494 2702 Fragments arranged by size: 2702 1964 1458 745 711 494 BsaBI GATnn'nnATC Cuts at: 3293 8068 3293 Size: 4775 3299 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2110 2226 2342 2380 Size: 53 83 186 936 577 116 116 38 Cuts at: 2380 3471 3684 4473 5881 6263 6898 275 Size: 1091 213 789 1408 382 635 1451 Fragments arranged by size: 1451 1408 1091 936 789 635 577 382 213 186 116 116 83 53 38 BsaWI w'CCGG_w Cuts at: 2235 4695 6507 6905 7179 7326 7737 2235 Size: 2460 1812 398 274 147 411 2572 Fragments arranged by size: 2572 2460 1812 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2423 2453 4023 4053 1178 Size: 30 165 30 1020 30 1570 30 5199 Fragments arranged by size: 5199 1570 1020 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2768 3719 4682 4691 4940 1186 Size: 1582 951 963 9 249 4320 Fragments arranged by size: 4320 1582 963 951 249 9 BseYI C'CCAG_C Cuts at: 2180 2266 2668 3063 3771 4426 4435 5360 7277 Size: 86 402 395 708 655 9 925 1917 Cuts at: 7277 7773 2180 Size: 496 2481 Fragments arranged by size: 2481 1917 925 708 655 496 402 395 86 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2122 2411 2708 2899 2920 4340 1163 Size: 573 386 289 297 191 21 1420 4897 Fragments arranged by size: 4897 1420 573 386 297 289 191 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5236 5524 6285 7313 1540 Size: 275 186 3235 288 761 1028 2301 Fragments arranged by size: 3235 2301 1028 761 288 275 186 BsiHKAI G_wGCw'C Cuts at: 3209 3243 3465 5091 6135 6220 7291 3209 Size: 34 222 1626 1044 85 1071 3992 Fragments arranged by size: 3992 1626 1071 1044 222 85 34 BsmI GAATG_Cn' Cuts at: 5585 5678 5585 Size: 93 7981 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2213 2358 2604 2747 3209 3243 Size: 399 411 12 145 246 143 462 34 Cuts at: 3243 3465 3863 4051 4243 4404 5091 5135 5161 Size: 222 398 188 192 161 687 44 26 Cuts at: 5161 5360 5393 6135 6220 7291 1391 Size: 199 33 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 687 462 411 399 398 246 222 199 192 188 161 145 143 85 44 34 33 26 12 BspEI T'CCGG_A Cuts at: 2235 7737 2235 Size: 5502 2572 BspHI T'CATG_A Cuts at: 3225 5963 7693 3225 Size: 2738 1730 3606 Fragments arranged by size: 3606 2738 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2117 2383 2680 3021 4597 5135 5429 844 Size: 1273 266 297 341 1576 538 294 3489 Fragments arranged by size: 3489 1576 1273 538 341 297 294 266 BsrBI CCG'CTC Cuts at: 1394 1823 3040 4233 4930 5022 5961 1394 Size: 429 1217 1193 697 92 939 3507 Fragments arranged by size: 3507 1217 1193 939 697 429 92 BsrDI GCAATG_nn' Cuts at: 65 1248 2095 4293 4768 4815 6569 6743 65 Size: 1183 847 2198 475 47 1754 174 1396 Fragments arranged by size: 2198 1754 1396 1183 847 475 174 47 BsrFI r'CCGG_y Cuts at: 1700 1810 2619 3960 6718 6905 1700 Size: 110 809 1341 2758 187 2869 Fragments arranged by size: 2869 2758 1341 809 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8074 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8074 BssSI C'ACGA_G Cuts at: 6134 7146 6134 Size: 1012 7062 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8074 BstBI TT'CG_AA Cuts at: 2007 6878 2007 Size: 4871 3203 BstEII G'GTnAC_C Cuts at: 1184 5245 1184 Size: 4061 4013 BstXI CCAn_nnnn'nTGG Cuts at: 2650 3400 3535 3751 4729 2650 Size: 750 135 216 978 5995 Fragments arranged by size: 5995 978 750 216 135 BstYI r'GATC_y Cuts at: 1972 3129 3294 3988 4018 4119 5404 5771 6156 Size: 1157 165 694 30 101 1285 367 385 Cuts at: 6156 6173 7625 7711 7723 8069 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1285 1157 694 385 367 346 165 101 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 3284 3882 4197 4984 3284 Size: 598 315 787 6374 Fragments arranged by size: 6374 787 598 315 BtgI C'CryG_G Cuts at: 513 1065 2113 2860 2986 3075 3622 3750 4477 Size: 552 1048 747 126 89 547 128 727 Cuts at: 4477 5250 513 Size: 773 3337 Fragments arranged by size: 3337 1048 773 747 727 552 547 128 126 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2749 4882 487 Size: 683 56 1523 2133 3679 Fragments arranged by size: 3679 2133 1523 683 56 BtsI GCAGTG_nn' Cuts at: 2286 2691 3207 3277 3355 3463 3581 5031 5590 Size: 405 516 70 78 108 118 1450 559 Cuts at: 5590 6384 6404 2286 Size: 794 20 3956 Fragments arranged by size: 3956 1450 794 559 516 405 118 108 78 70 20 ClaI AT'CG_AT Cuts at: 1554 3918 1554 Size: 2364 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4429 4464 529 Size: 35 3865 35 4139 Fragments arranged by size: 4139 3865 35 35 DraI TTT'AAA Cuts at: 5378 5378 Size: 8074 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7081 809 Size: 507 5765 1802 Fragments arranged by size: 5765 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2421 3525 3556 Size: 54 1425 50 275 18 591 1104 31 Cuts at: 3556 3720 4338 4380 5521 6410 8039 8 Size: 164 618 42 1141 889 1629 43 Fragments arranged by size: 1629 1425 1141 1104 889 618 591 275 164 54 50 43 42 31 18 EagI C'GGCC_G Cuts at: 1537 1812 5521 1537 Size: 275 3709 4090 Fragments arranged by size: 4090 3709 275 EarI CTCTTCn'nnn_ Cuts at: 1353 6004 1353 Size: 4651 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2621 3809 6661 7035 7181 387 Size: 1064 138 1032 1188 2852 374 146 1280 Fragments arranged by size: 2852 1280 1188 1064 1032 374 146 138 EcoICRI GAG'CTC Cuts at: 3241 3241 Size: 8074 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 4868 5312 6137 6734 7521 7789 Size: 393 818 2418 444 825 597 787 268 Cuts at: 7789 1239 Size: 1524 Fragments arranged by size: 2418 1524 825 818 787 597 444 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 5261 5261 Size: 8074 EcoO109I rG'GnC_Cy Cuts at: 2198 4201 4430 5227 5356 5357 2198 Size: 2003 229 797 129 1 4915 Fragments arranged by size: 4915 2003 797 229 129 1 EcoRI G'AATT_C Cuts at: 5383 5761 5383 Size: 378 7696 FspI TGC'GCA Cuts at: 3213 6580 3213 Size: 3367 4707 HaeII r_GCGC'y Cuts at: 1678 2113 2345 2383 2437 2703 2968 3687 4589 Size: 435 232 38 54 266 265 719 902 Cuts at: 4589 4759 7221 1678 Size: 170 2462 2531 Fragments arranged by size: 2531 2462 902 719 435 266 265 232 170 54 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3491 3523 3973 4005 5292 Size: 32 135 32 1919 32 450 32 1287 Cuts at: 5292 5324 6715 6747 6789 6821 1373 Size: 32 1391 32 42 32 2626 Fragments arranged by size: 2626 1919 1391 1287 450 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4617 5418 5664 669 Size: 988 281 2679 801 246 3079 Fragments arranged by size: 3079 2679 988 801 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8074 HpaI GTT'AAC Cuts at: 5664 5664 Size: 8074 KasI G'GCGC_C Cuts at: 2109 2341 2379 3683 2109 Size: 232 38 1304 6500 Fragments arranged by size: 6500 1304 232 38 KpnI G_GTAC'C Cuts at: 5399 5399 Size: 8074 MfeI C'AATT_G Cuts at: 5673 5673 Size: 8074 MluI A'CGCG_T Cuts at: 3639 7950 3639 Size: 4311 3763 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7188 7372 1305 Size: 5883 184 2007 Fragments arranged by size: 5883 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3527 3722 4340 4382 10 Size: 54 1425 343 1695 195 618 42 3702 Fragments arranged by size: 3702 1695 1425 618 343 195 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2513 2609 2648 2789 3080 3150 3202 Size: 648 1347 96 39 141 291 70 52 Cuts at: 3202 3360 3437 4109 4973 5082 6032 6391 6550 Size: 158 77 672 864 109 950 359 159 Cuts at: 6550 518 Size: 2042 Fragments arranged by size: 2042 1347 950 864 672 648 359 291 159 158 141 109 96 77 70 52 39 MspA1I CmG'CkG Cuts at: 1606 2321 2446 2513 2811 2988 3453 3733 3940 Size: 715 125 67 298 177 465 280 207 Cuts at: 3940 4526 4942 5290 5490 6167 7315 7560 1606 Size: 586 416 348 200 677 1148 245 2120 Fragments arranged by size: 2120 1148 715 677 586 465 416 348 298 280 245 207 200 177 125 67 NarI GG'CG_CC Cuts at: 2110 2342 2380 3684 2110 Size: 232 38 1304 6500 Fragments arranged by size: 6500 1304 232 38 NcoI C'CATG_G Cuts at: 513 1065 3075 3750 4477 513 Size: 552 2010 675 727 4110 Fragments arranged by size: 4110 2010 727 675 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8074 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8074 NotI GC'GGCC_GC Cuts at: 5521 5521 Size: 8074 NspI r_CATG'y Cuts at: 1132 1229 2794 3436 4306 4324 4601 5432 6977 Size: 97 1565 642 870 18 277 831 1545 Cuts at: 6977 1132 Size: 2229 Fragments arranged by size: 2229 1565 1545 870 831 642 277 97 18 PasI CC'CwG_GG Cuts at: 1348 2287 1348 Size: 939 7135 PciI A'CATG_T Cuts at: 4302 6973 4302 Size: 2671 5403 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 3623 4483 4744 1108 Size: 920 1595 860 261 4438 Fragments arranged by size: 4438 1595 920 860 261 PfoI T'CCnGG_A Cuts at: 2163 3794 4007 2163 Size: 1631 213 6230 Fragments arranged by size: 6230 1631 213 PmeI GTTT'AAAC Cuts at: 5378 5378 Size: 8074 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4916 4948 6143 6175 7656 7688 4916 Size: 32 1195 32 1481 32 5302 Fragments arranged by size: 5302 1481 1195 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4430 5227 4430 Size: 797 7277 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8074 PsiI TTA'TAA Cuts at: 5644 5644 Size: 8074 PspOMI G'GGCC_C Cuts at: 2197 5131 5356 2197 Size: 2934 225 4915 Fragments arranged by size: 4915 2934 225 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3587 3619 3587 Size: 32 8042 PstI C_TGCA'G Cuts at: 830 3018 3588 3732 4531 5426 830 Size: 2188 570 144 799 895 3478 Fragments arranged by size: 3478 2188 895 799 570 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8074 PvuII CAG'CTG Cuts at: 3940 4526 4942 5290 3940 Size: 586 416 348 6724 Fragments arranged by size: 6724 586 416 348 SacI G_AGCT'C Cuts at: 3243 3243 Size: 8074 SacII CC_GC'GG Cuts at: 2989 2989 Size: 8074 SalI G'TCGA_C Cuts at: 1936 4615 5416 1936 Size: 2679 801 4594 Fragments arranged by size: 4594 2679 801 SanDI GG'GwC_CC Cuts at: 4430 5227 4430 Size: 797 7277 SbfI CC_TGCA'GG Cuts at: 5426 5426 Size: 8074 ScaI AGT'ACT Cuts at: 4004 6322 8034 4004 Size: 2318 1712 4044 Fragments arranged by size: 4044 2318 1712 SexAI A'CCwGG_T Cuts at: 5262 5262 Size: 8074 SfcI C'TryA_G Cuts at: 826 1045 3014 3584 3728 4527 4646 4682 5422 Size: 219 1969 570 144 799 119 36 740 Cuts at: 5422 6557 7238 7429 826 Size: 1135 681 191 1471 Fragments arranged by size: 1969 1471 1135 799 740 681 570 219 191 144 119 36 SfiI GGCCn_nnn'nGGCC Cuts at: 3555 3719 3555 Size: 164 7910 SfoI GGC'GCC Cuts at: 2111 2343 2381 3685 2111 Size: 232 38 1304 6500 Fragments arranged by size: 6500 1304 232 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8074 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8074 SmaI CCC'GGG Cuts at: 1872 3341 4751 5401 1872 Size: 1469 1410 650 4545 Fragments arranged by size: 4545 1469 1410 650 SmlI C'TyrA_G Cuts at: 820 1017 1943 3647 3992 6178 7079 7341 820 Size: 197 926 1704 345 2186 901 262 1553 Fragments arranged by size: 2186 1704 1553 926 901 345 262 197 SnaBI TAC'GTA Cuts at: 493 5371 5865 493 Size: 4878 494 2702 Fragments arranged by size: 4878 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8074 SphI G_CATG'C Cuts at: 1132 2794 4601 5432 1132 Size: 1662 1807 831 3774 Fragments arranged by size: 3774 1807 1662 831 SrfI GCCC'GGGC Cuts at: 3341 3341 Size: 8074 SspI AAT'ATT Cuts at: 5 52 5998 5 Size: 47 5946 2081 Fragments arranged by size: 5946 2081 47 StuI AGG'CCT Cuts at: 1517 2972 3815 1517 Size: 1455 843 5776 Fragments arranged by size: 5776 1455 843 StyI C'CwwG_G Cuts at: 513 1065 3075 3475 3750 3801 3810 4477 4722 Size: 552 2010 400 275 51 9 667 245 Cuts at: 4722 5277 5513 513 Size: 555 236 3074 Fragments arranged by size: 3074 2010 667 555 552 400 275 245 236 51 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2194 2638 3878 5250 6101 6118 6271 Size: 1255 614 444 1240 1372 851 17 153 Cuts at: 6271 6456 6901 325 Size: 185 445 1498 Fragments arranged by size: 1498 1372 1255 1240 851 614 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4002 4173 4305 Size: 274 80 33 51 1443 2025 171 132 Cuts at: 4305 6320 8032 96 Size: 2015 1712 138 Fragments arranged by size: 2025 2015 1712 1443 274 171 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2630 2662 2929 2961 8034 8066 2630 Size: 32 267 32 5073 32 2638 Fragments arranged by size: 5073 2638 267 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3152 1316 Size: 1836 6238 XbaI T'CTAG_A Cuts at: 5410 5410 Size: 8074 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5059 5114 5059 Size: 55 8019 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8074 XmaI C'CCGG_G Cuts at: 1870 3339 4749 5399 1870 Size: 1469 1410 650 4545 Fragments arranged by size: 4545 1469 1410 650 XmnI GAAnn'nnTTC Cuts at: 2411 6203 2411 Size: 3792 4282 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5882 276 Size: 53 83 186 936 4348 2468 Fragments arranged by size: 4348 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SapI SwaI