(Circular) (Six-base) MAPSORT of: pFN21ASDA0410.seq Check: 3714 from: 1 to: 6184 pFN21ASDA0410 6184 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3527 1937 Size: 1590 4594 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2329 2768 2912 3043 3077 3215 4247 5631 Size: 697 439 144 131 34 138 1032 1384 Cuts at: 5631 5899 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1032 697 439 268 144 138 131 34 AflII C'TTAA_G Cuts at: 820 1017 2864 820 Size: 197 1847 4140 Fragments arranged by size: 4140 1847 197 AflIII A'CryG_T Cuts at: 1203 5083 6060 1203 Size: 3880 977 1327 Fragments arranged by size: 3880 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 2069 1166 Size: 903 5281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3465 1184 Size: 2281 3903 AlwNI CAG_nnn'CTG Cuts at: 2375 2902 3047 3927 5499 2375 Size: 527 145 880 1572 3060 Fragments arranged by size: 3060 1572 880 527 145 ApaLI G'TGCA_C Cuts at: 4241 5397 4241 Size: 1156 5028 ApoI r'AATT_y Cuts at: 1493 2801 3195 3493 3719 3871 5980 1493 Size: 1308 394 298 226 152 2109 1697 Fragments arranged by size: 2109 1697 1308 394 298 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 3509 1870 Size: 73 1566 4545 Fragments arranged by size: 4545 1566 73 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3505 4960 618 Size: 325 217 627 1718 1455 1842 Fragments arranged by size: 1842 1718 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2063 2148 2223 3503 1391 Size: 672 85 75 1280 4072 Fragments arranged by size: 4072 1280 672 85 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2841 3017 928 Size: 971 942 176 4095 Fragments arranged by size: 4095 971 942 176 BbvCI CC'TCA_GC Cuts at: 2410 2410 Size: 6184 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4375 4409 1903 Size: 34 2438 34 3678 Fragments arranged by size: 3678 2438 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4076 5286 4076 Size: 1210 4974 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4795 136 Size: 107 122 71 631 605 3123 1525 Fragments arranged by size: 3123 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6179 6179 Size: 6184 BlpI GC'TnA_GC Cuts at: 3601 3601 Size: 6184 Bme1580I G_kGCm'C Cuts at: 1790 4245 5401 1790 Size: 2455 1156 2573 Fragments arranged by size: 2573 2455 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2253 3915 4873 Size: 416 53 83 399 24 836 1662 958 Cuts at: 4873 442 Size: 1753 Fragments arranged by size: 1753 1662 958 836 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2418 1056 Size: 1362 4822 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2952 2952 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2163 2436 2780 4844 1239 Size: 924 273 344 2064 2579 Fragments arranged by size: 2579 2064 924 344 273 Bpu10I CC'TnA_GC Cuts at: 2410 2545 2970 2410 Size: 135 425 5624 Fragments arranged by size: 5624 425 135 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3126 3153 4309 5174 5472 3126 Size: 27 1156 865 298 3838 Fragments arranged by size: 3838 1156 865 298 27 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4847 882 Size: 617 3348 2219 Fragments arranged by size: 3348 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3481 3975 493 Size: 711 2277 494 2702 Fragments arranged by size: 2702 2277 711 494 BsaBI GATnn'nnATC Cuts at: 2757 6178 2757 Size: 3421 2763 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3991 4373 5008 275 Size: 53 83 186 936 2458 382 635 1451 Fragments arranged by size: 2458 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4617 5015 5289 5436 5847 4617 Size: 398 274 147 411 4954 Fragments arranged by size: 4954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2161 2179 2191 2209 2434 Size: 30 165 30 758 18 12 18 225 Cuts at: 2434 2464 3291 3321 1178 Size: 30 827 30 4041 Fragments arranged by size: 4041 827 758 225 165 30 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2683 1186 Size: 1497 4687 BseYI C'CCAG_C Cuts at: 5387 5883 5387 Size: 496 5688 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5611 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4245 4330 5401 4245 Size: 85 1071 5028 Fragments arranged by size: 5028 1071 85 BsmI GAATG_Cn' Cuts at: 2614 2989 3467 3695 3788 2614 Size: 375 478 228 93 5010 Fragments arranged by size: 5010 478 375 228 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2148 2223 3503 4245 4330 5401 Size: 399 273 85 75 1280 742 85 1071 Cuts at: 5401 1391 Size: 2174 Fragments arranged by size: 2174 1280 1071 742 399 273 85 85 75 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 3241 4073 5803 3241 Size: 832 1730 3622 Fragments arranged by size: 3622 1730 832 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2245 2374 3539 844 Size: 1401 129 1165 3489 Fragments arranged by size: 3489 1401 1165 129 BsrBI CCG'CTC Cuts at: 1394 1823 4071 1394 Size: 429 2248 3507 Fragments arranged by size: 3507 2248 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2665 2865 4679 4853 65 Size: 1183 1417 200 1814 174 1396 Fragments arranged by size: 1814 1417 1396 1183 200 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 4828 5015 1700 Size: 110 268 2750 187 2869 Fragments arranged by size: 2869 2750 268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 4244 5256 4244 Size: 1012 5172 BstAPI GCAn_nnn'nTGC Cuts at: 794 2375 3047 3413 794 Size: 1581 672 366 3565 Fragments arranged by size: 3565 1581 672 366 BstBI TT'CG_AA Cuts at: 2007 4988 2007 Size: 2981 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstXI CCAn_nnnn'nTGG Cuts at: 2071 2071 Size: 6184 BstYI r'GATC_y Cuts at: 1972 2228 3514 3881 4266 4283 5735 5821 5833 Size: 256 1286 367 385 17 1452 86 12 Cuts at: 5833 6179 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1286 385 367 346 256 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2070 3417 513 Size: 552 1005 1347 3280 Fragments arranged by size: 3280 1347 1005 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5445 Fragments arranged by size: 5445 683 56 BtsI GCAGTG_nn' Cuts at: 3700 4494 4514 3700 Size: 794 20 5370 Fragments arranged by size: 5370 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2368 2403 529 Size: 35 1804 35 4310 Fragments arranged by size: 4310 1804 35 35 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2431 5191 809 Size: 507 1115 2760 1802 Fragments arranged by size: 2760 1802 1115 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 3631 4520 Size: 54 1425 50 275 18 243 1558 889 Cuts at: 4520 6149 8 Size: 1629 43 Fragments arranged by size: 1629 1558 1425 889 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4114 1353 Size: 2761 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4771 5145 5291 387 Size: 1064 138 3182 374 146 1280 Fragments arranged by size: 3182 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2163 2329 2436 2768 2780 2912 3043 Size: 393 531 166 107 332 12 132 131 Cuts at: 3043 3077 3215 4247 4844 5631 5899 1239 Size: 34 138 1032 597 787 268 1524 Fragments arranged by size: 1524 1032 787 597 531 393 332 268 166 138 132 131 107 34 12 EcoNI CCTnn'n_nnAGG Cuts at: 3453 3453 Size: 6184 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2879 2879 Size: 6184 FspI TGC'GCA Cuts at: 4690 4690 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 5331 1678 Size: 3653 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2434 2466 4825 4857 4899 Size: 32 135 32 862 32 2359 32 42 Cuts at: 4899 4931 1373 Size: 32 2626 Fragments arranged by size: 2626 2359 862 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2622 3528 3774 669 Size: 988 281 684 906 246 3079 Fragments arranged by size: 3079 988 906 684 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5298 5482 1305 Size: 3993 184 2007 Fragments arranged by size: 3993 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4362 Fragments arranged by size: 4362 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2069 3036 3246 4142 4501 4660 518 Size: 648 903 967 210 896 359 159 2042 Fragments arranged by size: 2042 967 903 896 648 359 210 159 MspA1I CmG'CkG Cuts at: 1606 3600 4277 5425 5670 1606 Size: 1994 677 1148 245 2120 Fragments arranged by size: 2120 1994 1148 677 245 NaeI GCC'GGC Cuts at: 2080 2080 Size: 6184 NcoI C'CATG_G Cuts at: 513 1065 3417 513 Size: 552 2352 3280 Fragments arranged by size: 3280 2352 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NgoMIV G'CCGG_C Cuts at: 2078 2078 Size: 6184 NheI G'CTAG_C Cuts at: 1052 2414 1052 Size: 1362 4822 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 3542 5087 1132 Size: 97 2313 1545 2229 Fragments arranged by size: 2313 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6184 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 3345 1108 Size: 2237 3947 PfoI T'CCnGG_A Cuts at: 2048 2048 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PstI C_TGCA'G Cuts at: 830 2332 2371 2656 3536 830 Size: 1502 39 285 880 3478 Fragments arranged by size: 3478 1502 880 285 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 2170 4432 6144 2170 Size: 2262 1712 2210 Fragments arranged by size: 2262 2210 1712 SfcI C'TryA_G Cuts at: 826 1045 2292 2328 2367 2634 2652 3280 3453 Size: 219 1247 36 39 267 18 628 173 Cuts at: 3453 3532 4667 5348 5539 826 Size: 79 1135 681 191 1471 Fragments arranged by size: 1471 1247 1135 681 628 267 219 191 173 79 39 36 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 2078 1700 Size: 378 5806 SmaI CCC'GGG Cuts at: 1872 3511 1872 Size: 1639 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2864 3141 3168 4288 5189 5451 Size: 197 926 921 277 27 1120 901 262 Cuts at: 5451 820 Size: 1553 Fragments arranged by size: 1553 1120 926 921 901 277 262 197 27 SnaBI TAC'GTA Cuts at: 493 3481 3975 493 Size: 2988 494 2702 Fragments arranged by size: 2988 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 4108 5 Size: 47 4056 2081 Fragments arranged by size: 4056 2081 47 StuI AGG'CCT Cuts at: 1517 2526 2667 1517 Size: 1009 141 5034 Fragments arranged by size: 5034 1009 141 StyI C'CwwG_G Cuts at: 513 1065 2668 3274 3417 3623 513 Size: 552 1603 606 143 206 3074 Fragments arranged by size: 3074 1603 606 552 206 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2477 3373 4211 4228 4381 4566 5011 Size: 1255 897 896 838 17 153 185 445 Cuts at: 5011 325 Size: 1498 Fragments arranged by size: 1498 1255 897 896 838 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2168 2576 3099 Size: 274 80 33 51 1443 191 408 523 Cuts at: 3099 4430 6142 96 Size: 1331 1712 138 Fragments arranged by size: 1712 1443 1331 523 408 274 191 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6144 6176 6144 Size: 32 6152 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6184 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 3509 1870 Size: 1639 4545 XmnI GAAnn'nnTTC Cuts at: 2170 2848 3290 3467 4313 2170 Size: 678 442 177 846 4041 Fragments arranged by size: 4041 846 678 442 177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI