(Circular) (Six-base) MAPSORT of: pFN21ASDA0387.seq Check: 3760 from: 1 to: 7843 pFN21ASDA0387 7843 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2562 3694 2562 Size: 1132 6711 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3690 5653 278 Size: 53 83 186 936 2154 1963 2468 Fragments arranged by size: 2468 2154 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5186 1937 Size: 3249 4594 Acc65I G'GTAC_C Cuts at: 5164 5164 Size: 7843 AclI AA'CG_TT Cuts at: 1563 5970 6343 1563 Size: 4407 373 3063 Fragments arranged by size: 4407 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2472 3044 3384 4052 4328 4478 4787 5906 Size: 840 572 340 668 276 150 309 1119 Cuts at: 5906 7290 7558 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1119 840 668 572 340 309 276 268 150 AfeI AGC'GCT Cuts at: 4344 4344 Size: 7843 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7646 AflIII A'CryG_T Cuts at: 1203 2685 4149 6742 7719 1203 Size: 1482 1464 2593 977 1327 Fragments arranged by size: 2593 1482 1464 1327 977 AgeI A'CCGG_T Cuts at: 6674 6674 Size: 7843 AhdI GACnn_n'nnGTC Cuts at: 3310 6572 3310 Size: 3262 4581 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7843 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7843 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4703 4735 4703 Size: 32 7811 AlwNI CAG_nnn'CTG Cuts at: 2185 2335 4096 5586 7158 2185 Size: 150 1761 1490 1572 2870 Fragments arranged by size: 2870 1761 1572 1490 150 ApaI G_GGCC'C Cuts at: 2715 3531 4145 2715 Size: 816 614 6413 Fragments arranged by size: 6413 816 614 ApaLI G'TGCA_C Cuts at: 2901 5900 7056 2901 Size: 2999 1156 3688 Fragments arranged by size: 3688 2999 1156 ApoI r'AATT_y Cuts at: 1493 3855 5152 5378 5530 7639 1493 Size: 2362 1297 226 152 2109 1697 Fragments arranged by size: 2362 2109 1697 1297 226 152 AseI AT'TA_AT Cuts at: 160 1032 6397 160 Size: 872 5365 1606 Fragments arranged by size: 5365 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7843 AvaI C'yCGr_G Cuts at: 1870 1943 2213 2708 2864 3135 3287 3485 3659 Size: 73 270 495 156 271 152 198 174 Cuts at: 3659 4045 4446 4522 5168 1870 Size: 386 401 76 646 4545 Fragments arranged by size: 4545 646 495 401 386 271 270 198 174 156 152 76 73 BamHI G'GATC_C Cuts at: 5173 5540 5173 Size: 367 7476 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2271 2932 3706 4949 5164 Size: 325 217 627 484 661 774 1243 215 Cuts at: 5164 6619 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1243 774 661 627 484 325 217 215 BanII G_rGCy'C Cuts at: 1391 2715 2778 3334 3486 3510 3531 4145 4368 Size: 1324 63 556 152 24 21 614 223 Cuts at: 4368 5162 1391 Size: 794 4072 Fragments arranged by size: 4072 1324 794 614 556 223 152 63 24 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2985 3381 3390 3927 928 Size: 971 1086 396 9 537 4844 Fragments arranged by size: 4844 1086 971 537 396 9 BbvCI CC'TCA_GC Cuts at: 2637 3048 3575 5100 2637 Size: 411 527 1525 5380 Fragments arranged by size: 5380 1525 527 411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3703 3737 4049 4083 6034 6068 1903 Size: 34 1766 34 312 34 1951 34 3678 Fragments arranged by size: 3678 1951 1766 312 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5735 6945 5735 Size: 1210 6633 BclI T'GATC_A Cuts at: 1548 1548 Size: 7843 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3066 4358 4571 Size: 107 122 71 631 605 1394 1292 213 Cuts at: 4571 4947 6454 136 Size: 376 1507 1525 Fragments arranged by size: 1525 1507 1394 1292 631 605 376 213 122 107 71 BglII A'GATC_T Cuts at: 3454 7838 3454 Size: 4384 3459 BlpI GC'TnA_GC Cuts at: 2777 3090 5260 2777 Size: 313 2170 5360 Fragments arranged by size: 5360 2170 313 Bme1580I G_kGCm'C Cuts at: 1790 2715 2905 3531 3711 4145 4407 5904 7060 Size: 925 190 626 180 434 262 1497 1156 Cuts at: 7060 1790 Size: 2573 Fragments arranged by size: 2573 1497 1156 925 626 434 262 190 180 BmgBI CAC'GTC Cuts at: 1318 2596 2688 3730 4152 1318 Size: 1278 92 1042 422 5009 Fragments arranged by size: 5009 1278 1042 422 92 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4171 5128 5574 Size: 416 53 83 399 24 2754 957 446 Cuts at: 5574 6532 442 Size: 958 1753 Fragments arranged by size: 2754 1753 958 957 446 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4503 1056 Size: 3447 4396 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3859 4060 3859 Size: 201 7642 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2585 2747 2900 3092 3192 3348 3854 4346 Size: 1346 162 153 192 100 156 506 492 Cuts at: 4346 5045 6503 1239 Size: 699 1458 2579 Fragments arranged by size: 2579 1458 1346 699 506 492 192 162 156 153 100 Bpu10I CC'TnA_GC Cuts at: 2637 3048 3564 3575 5100 2637 Size: 411 516 11 1525 5380 Fragments arranged by size: 5380 1525 516 411 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2893 3875 4834 5053 5105 5968 6833 7131 2893 Size: 982 959 219 52 863 865 298 3605 Fragments arranged by size: 3605 982 959 865 863 298 219 52 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3318 3639 3958 4723 5038 6506 882 Size: 617 1819 321 319 765 315 1468 2219 Fragments arranged by size: 2219 1819 1468 765 617 321 319 315 BsaAI yAC'GTr Cuts at: 493 1204 2376 5140 5634 493 Size: 711 1172 2764 494 2702 Fragments arranged by size: 2764 2702 1172 711 494 BsaBI GATnn'nnATC Cuts at: 5009 7837 5009 Size: 2828 5015 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2446 2548 3687 3975 Size: 53 83 186 936 913 102 1139 288 Cuts at: 3975 4647 5650 6032 6667 275 Size: 672 1003 382 635 1451 Fragments arranged by size: 1451 1139 1003 936 913 672 635 382 288 186 102 83 53 BsaWI w'CCGG_w Cuts at: 3866 6276 6674 6948 7095 7506 3866 Size: 2410 398 274 147 411 4203 Fragments arranged by size: 4203 2410 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2494 2524 2558 2588 2639 Size: 30 165 30 1091 30 34 30 51 Cuts at: 2639 2669 2689 2719 2873 2903 4319 4349 4703 Size: 30 20 30 154 30 1416 30 354 Cuts at: 4703 4733 1178 Size: 30 4288 Fragments arranged by size: 4288 1416 1091 354 165 154 51 34 30 30 30 30 30 30 30 30 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2515 3422 3554 3894 4253 4409 4768 Size: 1045 284 907 132 340 359 156 359 Cuts at: 4768 4837 4848 5117 1186 Size: 69 11 269 3912 Fragments arranged by size: 3912 1045 907 359 359 340 284 269 156 132 69 11 BseYI C'CCAG_C Cuts at: 2464 2584 2721 2854 3460 3710 4199 7046 7542 Size: 120 137 133 606 250 489 2847 496 Cuts at: 7542 2464 Size: 2765 Fragments arranged by size: 2847 2765 606 496 489 250 137 133 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 3254 4089 4268 4956 1163 Size: 573 474 1044 835 179 688 4050 Fragments arranged by size: 4050 1044 835 688 573 474 179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5293 6054 7082 1540 Size: 275 186 3292 761 1028 2301 Fragments arranged by size: 3292 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2601 2778 2905 3007 3381 3510 3565 4728 5031 Size: 177 127 102 374 129 55 1163 303 Cuts at: 5031 5904 5989 7060 2601 Size: 873 85 1071 3384 Fragments arranged by size: 3384 1163 1071 873 374 303 177 129 127 102 85 55 BsmI GAATG_Cn' Cuts at: 2919 3475 5354 5447 2919 Size: 556 1879 93 5315 Fragments arranged by size: 5315 1879 556 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4174 4781 4174 Size: 607 7236 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2601 2715 2778 2905 3007 3334 3381 Size: 399 811 114 63 127 102 327 47 Cuts at: 3381 3486 3510 3531 3565 3711 4145 4368 4407 Size: 105 24 21 34 146 434 223 39 Cuts at: 4407 4728 5031 5162 5904 5989 7060 1391 Size: 321 303 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 811 742 434 399 327 321 303 223 146 131 127 114 105 102 85 63 47 39 34 24 21 BspEI T'CCGG_A Cuts at: 7506 7506 Size: 7843 BspHI T'CATG_A Cuts at: 5732 7462 5732 Size: 1730 6113 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2562 3039 3694 4782 5198 844 Size: 1718 477 655 1088 416 3489 Fragments arranged by size: 3489 1718 1088 655 477 416 BsrBI CCG'CTC Cuts at: 1394 1823 3155 4416 4602 4947 5730 1394 Size: 429 1332 1261 186 345 783 3507 Fragments arranged by size: 3507 1332 1261 783 429 345 186 BsrDI GCAATG_nn' Cuts at: 65 1248 2295 4921 6338 6512 65 Size: 1183 1047 2626 1417 174 1396 Fragments arranged by size: 2626 1417 1396 1183 1047 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3043 4271 4320 6487 6674 1700 Size: 110 1233 1228 49 2167 187 2869 Fragments arranged by size: 2869 2167 1233 1228 187 110 49 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7843 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7843 BssSI C'ACGA_G Cuts at: 2729 5903 6915 2729 Size: 3174 1012 3657 Fragments arranged by size: 3657 3174 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2335 794 Size: 1541 6302 BstBI TT'CG_AA Cuts at: 2007 6647 2007 Size: 4640 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7843 BstXI CCAn_nnnn'nTGG Cuts at: 2029 3524 2029 Size: 1495 6348 BstYI r'GATC_y Cuts at: 1972 3454 5173 5540 5925 5942 7394 7480 7492 Size: 1482 1719 367 385 17 1452 86 12 Cuts at: 7492 7838 1972 Size: 346 1977 Fragments arranged by size: 1977 1719 1482 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2441 2784 3906 4137 2441 Size: 343 1122 231 6147 Fragments arranged by size: 6147 1122 343 231 BtgI C'CryG_G Cuts at: 513 1065 2022 2319 2744 3124 3205 3307 4569 Size: 552 957 297 425 380 81 102 1262 Cuts at: 4569 5094 513 Size: 525 3262 Fragments arranged by size: 3262 1262 957 552 525 425 380 297 102 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3012 3922 4525 487 Size: 683 56 1786 910 603 3805 Fragments arranged by size: 3805 1786 910 683 603 56 BtsI GCAGTG_nn' Cuts at: 2045 2186 3756 4077 4661 4933 5359 6153 6173 Size: 141 1570 321 584 272 426 794 20 Cuts at: 6173 2045 Size: 3715 Fragments arranged by size: 3715 1570 794 584 426 321 272 141 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7843 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7808 DraI TTT'AAA Cuts at: 5147 5147 Size: 7843 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4835 6850 809 Size: 507 3519 2015 1802 Fragments arranged by size: 3519 2015 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2532 2755 4113 Size: 54 1425 50 275 18 702 223 1358 Cuts at: 4113 4121 4273 4670 4986 5290 6179 7808 8 Size: 8 152 397 316 304 889 1629 43 Fragments arranged by size: 1629 1425 1358 889 702 397 316 304 275 223 152 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 5290 1537 Size: 275 3478 4090 Fragments arranged by size: 4090 3478 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3399 3609 5773 1353 Size: 2046 210 2164 3423 Fragments arranged by size: 3423 2164 2046 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3197 3926 3998 4376 4478 6430 Size: 1064 138 1608 729 72 378 102 1952 Cuts at: 6430 6804 6950 387 Size: 374 146 1280 Fragments arranged by size: 1952 1608 1280 1064 729 378 374 146 138 102 72 EcoICRI GAG'CTC Cuts at: 2776 3508 2776 Size: 732 7111 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2472 2585 2747 2900 3044 3092 3192 Size: 393 840 113 162 153 144 48 100 Cuts at: 3192 3348 3384 3854 4052 4328 4346 4478 4787 Size: 156 36 470 198 276 18 132 309 Cuts at: 4787 5045 5906 6503 7290 7558 1239 Size: 258 861 597 787 268 1524 Fragments arranged by size: 1524 861 840 787 597 470 393 309 276 268 258 198 162 156 153 144 132 113 100 48 36 18 EcoNI CCTnn'n_nnAGG Cuts at: 2107 2213 2440 2576 3023 3299 3343 3701 4457 Size: 106 227 136 447 276 44 358 756 Cuts at: 4457 4849 2107 Size: 392 5101 Fragments arranged by size: 5101 756 447 392 358 276 227 136 106 44 EcoO109I rG'GnC_Cy Cuts at: 2221 2472 2569 2581 2607 2712 2734 2850 3028 Size: 251 97 12 26 105 22 116 178 Cuts at: 3028 3058 3292 3348 3528 3571 4141 4450 4854 Size: 30 234 56 180 43 570 309 404 Cuts at: 4854 5125 2221 Size: 271 4939 Fragments arranged by size: 4939 570 404 309 271 251 234 180 178 116 105 97 56 43 30 26 22 12 EcoRI G'AATT_C Cuts at: 5152 5530 5152 Size: 378 7465 EcoRV GAT'ATC Cuts at: 5011 5011 Size: 7843 FspI TGC'GCA Cuts at: 6349 6349 Size: 7843 HaeII r_GCGC'y Cuts at: 1678 2335 3771 4224 4346 6990 1678 Size: 657 1436 453 122 2644 2531 Fragments arranged by size: 2644 2531 1436 657 453 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2494 2526 2558 2590 2639 Size: 32 135 32 922 32 32 32 49 Cuts at: 2639 2671 2689 2721 3296 3328 4667 4699 4747 Size: 32 18 32 575 32 1339 32 48 Cuts at: 4747 4779 6484 6516 6558 6590 1373 Size: 32 1705 32 42 32 2626 Fragments arranged by size: 2626 1705 1339 922 575 135 49 48 42 32 32 32 32 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3817 5187 5433 669 Size: 988 281 1879 1370 246 3079 Fragments arranged by size: 3079 1879 1370 988 281 246 HindIII A'AGCT_T Cuts at: 748 2354 748 Size: 1606 6237 HpaI GTT'AAC Cuts at: 5433 5433 Size: 7843 KpnI G_GTAC'C Cuts at: 5168 5168 Size: 7843 MfeI C'AATT_G Cuts at: 3590 5442 3590 Size: 1852 5991 MluI A'CGCG_T Cuts at: 7719 7719 Size: 7843 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2569 4278 6957 7141 1305 Size: 1264 1709 2679 184 2007 Fragments arranged by size: 2679 2007 1709 1264 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 4115 4988 10 Size: 54 1425 343 702 1581 873 2865 Fragments arranged by size: 2865 1581 1425 873 702 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 4701 4926 5801 6160 6319 518 Size: 648 861 2674 225 875 359 159 2042 Fragments arranged by size: 2674 2042 875 861 648 359 225 159 MspA1I CmG'CkG Cuts at: 1606 2636 3494 3557 4558 5259 5936 7084 7329 Size: 1030 858 63 1001 701 677 1148 245 Cuts at: 7329 1606 Size: 2120 Fragments arranged by size: 2120 1148 1030 1001 858 701 677 245 63 NaeI GCC'GGC Cuts at: 3045 3045 Size: 7843 NcoI C'CATG_G Cuts at: 513 1065 2022 3124 513 Size: 552 957 1102 5232 Fragments arranged by size: 5232 1102 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7843 NgoMIV G'CCGG_C Cuts at: 3043 3043 Size: 7843 NheI G'CTAG_C Cuts at: 1052 4499 1052 Size: 3447 4396 NotI GC'GGCC_GC Cuts at: 5290 5290 Size: 7843 NspI r_CATG'y Cuts at: 1132 1229 5201 6746 1132 Size: 97 3972 1545 2229 Fragments arranged by size: 3972 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2109 3146 3160 4549 1348 Size: 761 1037 14 1389 4642 Fragments arranged by size: 4642 1389 1037 761 14 PciI A'CATG_T Cuts at: 6742 6742 Size: 7843 PflMI CCAn_nnn'nTGG Cuts at: 1108 2818 4111 1108 Size: 1710 1293 4840 Fragments arranged by size: 4840 1710 1293 PfoI T'CCnGG_A Cuts at: 2562 4860 2562 Size: 2298 5545 PmeI GTTT'AAAC Cuts at: 5147 5147 Size: 7843 PmlI CAC'GTG Cuts at: 2376 2376 Size: 7843 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4703 4735 5912 5944 7425 7457 4703 Size: 32 1177 32 1481 32 5089 Fragments arranged by size: 5089 1481 1177 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2607 2734 3028 4450 4854 2607 Size: 127 294 1422 404 5596 Fragments arranged by size: 5596 1422 404 294 127 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7843 PsiI TTA'TAA Cuts at: 5413 5413 Size: 7843 PspOMI G'GGCC_C Cuts at: 2711 3527 4141 2711 Size: 816 614 6413 Fragments arranged by size: 6413 816 614 PspXI vC'TCGA_Gb Cuts at: 2213 3135 2213 Size: 922 6921 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4520 4552 4520 Size: 32 7811 PstI C_TGCA'G Cuts at: 830 2331 3036 3303 3705 4779 5195 830 Size: 1501 705 267 402 1074 416 3478 Fragments arranged by size: 3478 1501 1074 705 416 402 267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7843 SacI G_AGCT'C Cuts at: 2778 3510 2778 Size: 732 7111 SalI G'TCGA_C Cuts at: 1936 5185 1936 Size: 3249 4594 SbfI CC_TGCA'GG Cuts at: 3303 3705 5195 3303 Size: 402 1490 5951 Fragments arranged by size: 5951 1490 402 ScaI AGT'ACT Cuts at: 6091 7803 6091 Size: 1712 6131 SexAI A'CCwGG_T Cuts at: 4713 4713 Size: 7843 SfcI C'TryA_G Cuts at: 826 1045 2327 3032 3299 3701 4775 4891 5191 Size: 219 1282 705 267 402 1074 116 300 Cuts at: 5191 6326 7007 7198 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1282 1135 1074 705 681 402 300 267 219 191 116 SfiI GGCCn_nnn'nGGCC Cuts at: 3066 3066 Size: 7843 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7843 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7843 SmaI CCC'GGG Cuts at: 1872 2710 2866 3487 4448 5170 1872 Size: 838 156 621 961 722 4545 Fragments arranged by size: 4545 961 838 722 621 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 2213 2908 3135 3890 4849 5032 Size: 197 926 270 695 227 755 959 183 Cuts at: 5032 5120 5947 6848 7110 820 Size: 88 827 901 262 1553 Fragments arranged by size: 1553 959 926 901 827 755 695 270 262 227 197 183 88 SnaBI TAC'GTA Cuts at: 493 5140 5634 493 Size: 4647 494 2702 Fragments arranged by size: 4647 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7843 SphI G_CATG'C Cuts at: 1132 5201 1132 Size: 4069 3774 SspI AAT'ATT Cuts at: 5 52 2042 5767 5 Size: 47 1990 3725 2081 Fragments arranged by size: 3725 2081 1990 47 StuI AGG'CCT Cuts at: 1517 2243 2923 1517 Size: 726 680 6437 Fragments arranged by size: 6437 726 680 StyI C'CwwG_G Cuts at: 513 1065 2022 3124 3192 5282 513 Size: 552 957 1102 68 2090 3074 Fragments arranged by size: 3074 2090 1102 957 552 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4146 4562 4845 5870 5887 6040 6225 Size: 1255 2566 416 283 1025 17 153 185 Cuts at: 6225 6670 325 Size: 445 1498 Fragments arranged by size: 2566 1498 1255 1025 445 416 283 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6089 7801 96 Size: 274 80 33 51 1443 4112 1712 138 Fragments arranged by size: 4112 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2159 2191 3735 3767 4319 4331 4351 4363 7803 Size: 32 1544 32 552 12 20 12 3440 Cuts at: 7803 7835 2159 Size: 32 2167 Fragments arranged by size: 3440 2167 1544 552 32 32 32 20 12 12 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7843 XbaI T'CTAG_A Cuts at: 5179 5179 Size: 7843 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2926 3457 3796 2926 Size: 531 339 6973 Fragments arranged by size: 6973 531 339 XhoI C'TCGA_G Cuts at: 1943 2213 3135 1943 Size: 270 922 6651 Fragments arranged by size: 6651 922 270 XmaI C'CCGG_G Cuts at: 1870 2708 2864 3485 4446 5168 1870 Size: 838 156 621 961 722 4545 Fragments arranged by size: 4545 961 838 722 621 156 XmnI GAAnn'nnTTC Cuts at: 2084 5972 2084 Size: 3888 3955 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3688 5651 276 Size: 53 83 186 936 2154 1963 2468 Fragments arranged by size: 2468 2154 1963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII FalI FseI FspAI KasI NarI NruI NsiI PacI PvuII RsrII SacII SanDI SapI SfoI SrfI SwaI