Restriction map for
pFN21ASDA0383
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BglI BglII
BlnI BsaAI BsaBI BseAI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
KasI KspI MamI MluI MroI
NarI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI SacI SacII
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1277 GGTACC G^GTACC
AclI 1569 AACGTT AA^CGTT
AcyI 3556 GGCGTC G[GA]^CG[CT]C
AflII 783 CTTAAG C^TTAAG
AflIII 3109 ACATGT A^C[GA][CT]GT
AocI 3172 CCTGAGG CC^T[ACGT]AGG
Asp718I 1277 GGTACC G^GTACC
AspHI 4146 GAGCAC G[AT]GC[AT]^C
AvaI 4477 CCCGAG C^[CT]CG[GA]G
BamHI 1081 GGATCC G^GATCC
BcgI 3683 GCAAGGAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 783 CTTAAG C^TTAAG
BlpI 2146 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2146 GCTGAGC GC^T[ACGT]AGC
BsaHI 3556 GGCGTC G[GA]^CG[CT]C
BsaWI 2923 ACCGGA [AT]^CCGG[AT]
BsiEI 1030 CGACCG CG[GA][CT]^CG
BsiHKAI 4146 GAGCAC G[AT]GC[AT]^C
BsiWI 3954 CGTACG C^GTACG
BsoBI 4477 CCCGAG C^[CT]CG[GA]G
BspHI 981 TCATGA T^CATGA
BspLU11I 3109 ACATGT A^CATGT
BsrBI 2149 GAGCGG CCGCTC(-3/-3)
BstEII 1109 GGTCACC G^GT[ACGT]ACC
BstPI 1109 GGTCACC G^GT[ACGT]ACC
BstXI 4457 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3172 CCTGAGG CC^T[ACGT]AGG
CelII 2146 GCTGAGC GC^T[ACGT]AGC
CpoI 504 CGGACCG CG^G[AT]CCG
DraI 2929 TTTAAA TTT^AAA
DraIII 3137 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3550 GACTGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3172 CCTGAGG CC^T[ACGT]AGG
EcoNI 1650 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1109 GGTCACC G^GT[ACGT]ACC
EcoRI 1378 GAATTC G^AATTC
EcoT22I 1091 ATGCAT ATGCA^T
Hin1I 3556 GGCGTC G[GA]^CG[CT]C
HpaI 4590 GTTAAC GTT^AAC
KpnI 1277 GGTACC GGTAC^C
MfeI 341 CAATTG C^AATTG
MunI 341 CAATTG C^AATTG
NaeI 2276 GCCGGC GCC^GGC
NgoMIV 2276 GCCGGC G^CCGGC
NheI 2167 GCTAGC G^CTAGC
NsiI 1091 ATGCAT ATGCA^T
PshAI 3859 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1569 AACGTT AA^CGTT
RcaI 981 TCATGA T^CATGA
RsrII 504 CGGACCG CG^G[AT]CCG
SalI 3559 GTCGAC G^TCGAC
SphI 4322 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3559 GTCGAC GT^[AC][GT]AC
4111 GTAGAC
AccII 472 CGCG CG^CG
2884 CGCG
Asp700I 1089 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2434 GAAAATATTC
BalI 2866 TGGCCA TGG^CCA
3945 TGGCCA
BanII 2596 GAGCCC G[GA]GC[CT]^C
3586 GAGCCC
BciVI 1645 GGATAC GTATCC(6/5)
558 GTATCC
BcnI 2268 CCCGG CC^[GC]GG
5275 CCCGG
BsaI 2563 GAGACC GGTCTC(1/5)
1787 GGTCTC
Bsp1407I 2942 TGTACA T^GTACA
5264 TGTACA
BsrFI 1274 GCCGGT [GA]^CCGG[CT]
2276 GCCGGC
BsrGI 2942 TGTACA T^GTACA
5264 TGTACA
BstUI 472 CGCG CG^CG
2884 CGCG
BstYI 1081 GGATCC [GA]^GATC[CT]
3264 AGATCC
Cfr10I 1274 GCCGGT [GA]^CCGG[CT]
2276 GCCGGC
MflI 1081 GGATCC [GA]^GATC[CT]
3264 AGATCC
MluNI 2866 TGGCCA TGG^CCA
3945 TGGCCA
MscI 2866 TGGCCA TGG^CCA
3945 TGGCCA
MvnI 472 CGCG CG^CG
2884 CGCG
NciI 2268 CCCGG CC^[GC]GG
5275 CCCGG
NdeI 886 CATATG CA^TATG
5219 CATATG
PflMI 117 CCATGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4413 CCAGCAGCTGG
SexAI 1318 ACCTGGT A^CC[AT]GGT
3138 ACCAGGT
SspBI 2942 TGTACA T^GTACA
5264 TGTACA
Van91I 117 CCATGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4413 CCAGCAGCTGG
XbaI 283 TCTAGA T^CTAGA
1765 TCTAGA
XhoII 1081 GGATCC [GA]^GATC[CT]
3264 AGATCC
XmnI 1089 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2434 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanI BbsI
BbvI BclI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI