Restriction map for
pFN21ASDA0355
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BbeI BbrPI BciVI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 2646 CGCG CG^CG
AcyI 1057 GGCGTC G[GA]^CG[CT]C
AflII 1096 CTTAAG C^TTAAG
AhdI 1422 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1422 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 1926 TGATCA T^GATCA
BfrI 1096 CTTAAG C^TTAAG
BglI 2603 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1057 GGCGTC G[GA]^CG[CT]C
BsmBI 1059 CGTCTC CGTCTC(1/5)
BsmI 2486 GAATGC GAATGC(1/-1)
Bsp1407I 2363 TGTACA T^GTACA
BsrDI 889 GCAATG GCAATG(2/0)
BsrGI 2363 TGTACA T^GTACA
BstUI 2646 CGCG CG^CG
EagI 1076 CGGCCG C^GGCCG
Eam1105I 1422 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1076 CGGCCG C^GGCCG
Eco52I 1076 CGGCCG C^GGCCG
FbaI 1926 TGATCA T^GATCA
HaeII 44 GGCGCT [GA]GCGC^[CT]
Hin1I 1057 GGCGTC G[GA]^CG[CT]C
HincII 1270 GTTGAC GT[CT]^[GA]AC
HindII 1270 GTTGAC GT[CT]^[GA]AC
MvnI 2646 CGCG CG^CG
NcoI 2030 CCATGG C^CATGG
NspI 366 GCATGT [GA]CATG^[CT]
PaeR7I 2736 CTCGAG C^TCGAG
PpuMI 2433 GGGACCT [GA]G^G[AT]CC[CT]
SacI 1012 GAGCTC GAGCT^C
ScaI 280 AGTACT AGT^ACT
SexAI 1190 ACCAGGT A^CC[AT]GGT
SmaI 2618 CCCGGG CCC^GGG
SspBI 2363 TGTACA T^GTACA
SspI 1747 AATATT AAT^ATT
StuI 3161 AGGCCT AGG^CCT
XhoI 2736 CTCGAG C^TCGAG
XmaCI 2618 CCCGGG C^CCGGG
XmaI 2618 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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BcgI 1213 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3072 CGACTGCAGTGC
BglII 294 AGATCT A^GATCT
1529 AGATCT
BseRI 2318 CTCCTC GAGGAG(10/8)
2875 CTCCTC
BsiEI 1076 CGGCCG CG[GA][CT]^CG
2598 CGGTCG
BspMI 1556 ACCTGC ACCTGC(4/8)
2308 GCAGGT
DraII 2433 GGGACCT [GA]G^G[ACGT]CC[CT]
2790 AGGGCCT
DraIII 119 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1356 CACATGGTG
EarI 298 CTCTTC CTCTTC(1/4)
1723 GAAGAG
EcoO109I 2433 GGGACCT [GA]G^G[ACGT]CC[CT]
2790 AGGGCCT
EcoRI 472 GAATTC G^AATTC
1824 GAATTC
EcoT14I 2030 CCATGG C^C[AT][AT]GG
3149 CCAAGG
Ksp632I 298 CTCTTC CTCTTC(1/4)
1723 GAAGAG
NaeI 898 GCCGGC GCC^GGC
2603 GCCGGC
NgoMIV 898 GCCGGC G^CCGGC
2603 GCCGGC
PflMI 567 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2915 CCAAAACGTGG
StyI 2030 CCATGG C^C[AT][AT]GG
3149 CCAAGG
TaqI 830 TCGA T^CGA
2737 TCGA
TthHB8I 830 TCGA T^CGA
2737 TCGA
Van91I 567 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2915 CCAAAACGTGG
XbaI 712 TCTAGA T^CTAGA
1330 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmnI