Restriction map for
pFN21ASDA0332
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AclI AcyI AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AvaI AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BglI BlnI
BpmI BsaHI BsgI BsiEI BsiWI
BsmBI BsoBI BspHI BspLU11I BspMI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV FseI HaeII Hin1I
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MluNI MscI
MunI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tsp45I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 2822 GTAGAC GT^[AC][GT]AC
AccII 738 CGCG CG^CG
AccIII 1135 TCCGGA T^CCGGA
AflII 1600 CTTAAG C^TTAAG
Alw44I 2114 GTGCAC G^TGCAC
ApaLI 2114 GTGCAC G^TGCAC
AseI 841 ATTAAT AT^TAAT
AsnI 841 ATTAAT AT^TAAT
Asp700I 1474 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1152 TGCGCA TGC^GCA
BanI 2086 GGTGCC G^G[CT][GA]CC
BclI 2003 TGATCA T^GATCA
BfrI 1600 CTTAAG C^TTAAG
BlpI 274 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 274 GCTAAGC GC^T[ACGT]AGC
BsaAI 1316 TACGTG [CT]AC^GT[GA]
BsaI 3056 GGTCTC GGTCTC(1/5)
BsaWI 1135 TCCGGA [AT]^CCGG[AT]
BseAI 1135 TCCGGA T^CCGGA
BsmFI 2179 GTCCC GGGAC(10/14)
BsmI 247 GCATTC GAATGC(1/-1)
Bsp1407I 1805 TGTACA T^GTACA
BspDI 2161 ATCGAT AT^CGAT
BspEI 1135 TCCGGA T^CCGGA
BsrBI 1165 GAGCGG CCGCTC(-3/-3)
BsrDI 1675 GCAATG GCAATG(2/0)
BsrGI 1805 TGTACA T^GTACA
BstUI 738 CGCG CG^CG
CelII 274 GCTAAGC GC^T[ACGT]AGC
ClaI 2161 ATCGAT AT^CGAT
EcoRI 2138 GAATTC G^AATTC
EcoT22I 95 ATGCAT ATGCA^T
FauI 1638 GCGGG CCCGC(4/6)
FbaI 2003 TGATCA T^GATCA
FspI 1152 TGCGCA TGC^GCA
HapII 1136 CCGG C^CGG
HpaII 1136 CCGG C^CGG
MroI 1135 TCCGGA T^CCGGA
MspA1I 335 CAGCTG C[AC]G^C[GT]G
MspI 1136 CCGG C^CGG
MvnI 738 CGCG CG^CG
NdeI 88 CATATG CA^TATG
NsiI 95 ATGCAT ATGCA^T
PshBI 841 ATTAAT AT^TAAT
PstI 1441 CTGCAG CTGCA^G
PvuII 335 CAGCTG CAG^CTG
ScaI 2495 AGTACT AGT^ACT
SspBI 1805 TGTACA T^GTACA
XbaI 2998 TCTAGA T^CTAGA
XmnI 1474 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AlwNI 335 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
707 CAGATTCTG
AspI 1230 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2475 GACTGAGTC
BsaBI 2197 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2951 GATCACCATC
BsaJI 831 CCTTGG C^C[ACGT][ACGT]GG
1651 CCAAGG
BsiYI 1129 CCTCCATCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2091 CCCCATCGAGG
BslI 1129 CCTCCATCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2091 CCCCATCGAGG
Cac8I 1239 GCAAGC GC[ACGT]^[ACGT]GC
1281 GCTCGC
CfoI 739 GCGC GCG^C
1153 GCGC
DraI 1002 TTTAAA TTT^AAA
2260 TTTAAA
EcoT14I 831 CCTTGG C^C[AT][AT]GG
1651 CCAAGG
HgaI 736 GACGC GACGC(5/10)
723 GCGTC
HhaI 739 GCGC GCG^C
1153 GCGC
HinP1I 739 GCGC G^CGC
1153 GCGC
HindIII 1426 AAGCTT A^AGCTT
1565 AAGCTT
HphI 2842 TCACC GGTGA(8/7)
2953 TCACC
MaeII 651 ACGT A^CGT
1317 ACGT
MaeIII 1194 GTTAC ^GT[ACGT]AC
2320 GTTAC
MamI 2197 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2951 GATCACCATC
MslI 88 CATATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2273 CATCAAAATG
PflFI 1230 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2475 GACTGAGTC
SacI 2542 GAGCTC GAGCT^C
2889 GAGCTC
SapI 1549 GAAGAGC GCTCTTC(1/4)
2101 GAAGAGC
SmlI 216 CTCAAG C^T[CT][GA]AG
1600 CTTAAG
SspI 2017 AATATT AAT^ATT
2038 AATATT
StyI 831 CCTTGG C^C[AT][AT]GG
1651 CCAAGG
TspRI 903 CAGTG CA[GC]TG[ACGT][ACGT]^
2817 CAGTG
Tth111I 1230 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2475 GACTGAGTC
XcmI 1244 CCATAGTCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1778 CCAGATTATATTTGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BglII BmyI BpuAI
BseRI BsiHKAI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TthHB8I
XhoII