(Circular) (Six-base) MAPSORT of: pFN21ASDA0332.seq Check: 4191 from: 1 to: 7816 pFN21ASDA0332 7816 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5626 278 Size: 53 83 186 936 4090 2468 Fragments arranged by size: 4090 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4846 5159 1937 Size: 2909 313 4594 Fragments arranged by size: 4594 2909 313 Acc65I G'GTAC_C Cuts at: 5137 5137 Size: 7816 AclI AA'CG_TT Cuts at: 1563 5943 6316 1563 Size: 4380 373 3063 Fragments arranged by size: 4380 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2085 3744 3753 3822 3967 4275 4476 5879 Size: 453 1659 9 69 145 308 201 1403 Cuts at: 5879 7263 7531 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1659 1403 1384 453 308 268 201 145 69 9 AflII C'TTAA_G Cuts at: 820 1017 3623 820 Size: 197 2606 5013 Fragments arranged by size: 5013 2606 197 AflIII A'CryG_T Cuts at: 1203 6715 7692 1203 Size: 5512 977 1327 Fragments arranged by size: 5512 1327 977 AgeI A'CCGG_T Cuts at: 6647 6647 Size: 7816 AhdI GACnn_n'nnGTC Cuts at: 6545 6545 Size: 7816 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7816 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7816 AlwNI CAG_nnn'CTG Cuts at: 2363 2735 5559 7131 2363 Size: 372 2824 1572 3048 Fragments arranged by size: 3048 2824 1572 372 ApaLI G'TGCA_C Cuts at: 4137 5873 7029 4137 Size: 1736 1156 4924 Fragments arranged by size: 4924 1736 1156 ApoI r'AATT_y Cuts at: 1493 2661 2900 3031 3298 3872 4161 5125 5351 Size: 1168 239 131 267 574 289 964 226 Cuts at: 5351 5503 7612 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1168 964 574 289 267 239 226 152 131 AseI AT'TA_AT Cuts at: 160 1032 2865 6370 160 Size: 872 1833 3505 1606 Fragments arranged by size: 3505 1833 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7816 AvaI C'yCGr_G Cuts at: 1870 1943 5141 1870 Size: 73 3198 4545 Fragments arranged by size: 4545 3198 73 BamHI G'GATC_C Cuts at: 5146 5513 5146 Size: 367 7449 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4109 5137 6592 618 Size: 325 217 627 2322 1028 1455 1842 Fragments arranged by size: 2322 1842 1455 1028 627 325 217 BanII G_rGCy'C Cuts at: 1391 2171 4569 4916 5135 1391 Size: 780 2398 347 219 4072 Fragments arranged by size: 4072 2398 780 347 219 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2535 3257 4263 928 Size: 971 636 722 1006 4481 Fragments arranged by size: 4481 1006 971 722 636 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6007 6041 1903 Size: 34 4070 34 3678 Fragments arranged by size: 4070 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5708 6918 5708 Size: 1210 6606 BclI T'GATC_A Cuts at: 1548 4026 1548 Size: 2478 5338 BfrBI ATG'CAT Cuts at: 2120 2120 Size: 7816 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6427 136 Size: 107 122 71 631 605 4755 1525 Fragments arranged by size: 4755 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4898 5024 5066 7811 4898 Size: 126 42 2745 4903 Fragments arranged by size: 4903 2745 126 42 BlpI GC'TnA_GC Cuts at: 2298 5233 2298 Size: 2935 4881 Bme1580I G_kGCm'C Cuts at: 1790 2203 4114 4141 5877 7033 1790 Size: 413 1911 27 1736 1156 2573 Fragments arranged by size: 2573 1911 1736 1156 413 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7816 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5053 5547 6505 Size: 416 53 83 399 24 3636 494 958 Cuts at: 6505 442 Size: 1753 Fragments arranged by size: 3636 1753 958 494 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7816 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6476 1239 Size: 5237 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2224 5941 6806 7104 2224 Size: 3717 865 298 2936 Fragments arranged by size: 3717 2936 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5085 6479 882 Size: 617 3586 1394 2219 Fragments arranged by size: 3586 2219 1394 617 BsaAI yAC'GTr Cuts at: 493 1204 3341 5113 5607 493 Size: 711 2137 1772 494 2702 Fragments arranged by size: 2702 2137 1772 711 494 BsaBI GATnn'nnATC Cuts at: 4224 4978 7810 4224 Size: 754 2832 4230 Fragments arranged by size: 4230 2832 754 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5623 6005 6640 275 Size: 53 83 186 936 4090 382 635 1451 Fragments arranged by size: 4090 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3158 6249 6647 6921 7068 7479 3158 Size: 3091 398 274 147 411 3495 Fragments arranged by size: 3495 3091 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7591 Fragments arranged by size: 7591 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2214 2390 2712 1186 Size: 1028 176 322 6290 Fragments arranged by size: 6290 1028 322 176 BseYI C'CCAG_C Cuts at: 4820 7019 7515 4820 Size: 2199 496 5121 Fragments arranged by size: 5121 2199 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7243 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5266 6027 7055 1540 Size: 275 186 3265 761 1028 2301 Fragments arranged by size: 3265 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3622 4141 4569 4916 5877 5962 7033 3622 Size: 519 428 347 961 85 1071 4405 Fragments arranged by size: 4405 1071 961 519 428 347 85 BsmI GAATG_Cn' Cuts at: 2270 5327 5420 2270 Size: 3057 93 4666 Fragments arranged by size: 4666 3057 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2171 2203 3622 4114 4141 4569 4916 Size: 399 381 32 1419 492 27 428 347 Cuts at: 4916 5135 5877 5962 7033 1391 Size: 219 742 85 1071 2174 Fragments arranged by size: 2174 1419 1071 742 492 428 399 381 347 219 85 32 27 BspEI T'CCGG_A Cuts at: 3158 7479 3158 Size: 4321 3495 BspHI T'CATG_A Cuts at: 5705 7435 5705 Size: 1730 6086 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5171 844 Size: 4327 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3190 5703 1394 Size: 429 1367 2513 3507 Fragments arranged by size: 3507 2513 1367 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3705 6311 6485 65 Size: 1183 2457 2606 174 1396 Fragments arranged by size: 2606 2457 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6460 6647 1700 Size: 110 4650 187 2869 Fragments arranged by size: 4650 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3828 96 Size: 3732 4084 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7816 BssSI C'ACGA_G Cuts at: 5876 6888 5876 Size: 1012 6804 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7816 BstBI TT'CG_AA Cuts at: 2007 6620 2007 Size: 4613 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7816 BstXI CCAn_nnnn'nTGG Cuts at: 2903 3486 4302 2903 Size: 583 816 6417 Fragments arranged by size: 6417 816 583 BstYI r'GATC_y Cuts at: 1972 2045 2096 2830 3521 4898 5024 5054 5066 Size: 73 51 734 691 1377 126 30 12 Cuts at: 5066 5146 5513 5898 5915 7367 7453 7465 7811 Size: 80 367 385 17 1452 86 12 346 Cuts at: 7811 1972 Size: 1977 Fragments arranged by size: 1977 1452 1377 734 691 385 367 346 126 86 80 73 51 30 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 6307 Fragments arranged by size: 6307 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7077 Fragments arranged by size: 7077 683 56 BtsI GCAGTG_nn' Cuts at: 4846 5332 6126 6146 4846 Size: 486 794 20 6516 Fragments arranged by size: 6516 794 486 20 ClaI AT'CG_AT Cuts at: 1554 4185 1554 Size: 2631 5185 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7781 DraI TTT'AAA Cuts at: 3027 4285 5120 3027 Size: 1258 835 5723 Fragments arranged by size: 5723 1258 835 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6823 809 Size: 507 5507 1802 Fragments arranged by size: 5507 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5263 6152 7781 Size: 54 1425 50 275 18 3433 889 1629 Cuts at: 7781 8 Size: 43 Fragments arranged by size: 3433 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5263 1537 Size: 275 3451 4090 Fragments arranged by size: 4090 3451 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 2571 2645 3001 3567 3841 4119 4793 Size: 900 318 74 356 566 274 278 674 Cuts at: 4793 5746 1353 Size: 953 3423 Fragments arranged by size: 3423 953 900 674 566 356 318 278 274 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 2058 3108 3505 6403 6777 Size: 1064 138 453 16 1050 397 2898 374 Cuts at: 6777 6923 387 Size: 146 1280 Fragments arranged by size: 2898 1280 1064 1050 453 397 374 146 138 16 EcoICRI GAG'CTC Cuts at: 4567 4914 4567 Size: 347 7469 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 3744 3753 3822 3967 4275 4476 Size: 393 453 1659 9 69 145 308 201 Cuts at: 4476 5879 6476 7263 7531 1239 Size: 1403 597 787 268 1524 Fragments arranged by size: 1659 1524 1403 787 597 453 393 308 268 201 145 69 9 EcoRI G'AATT_C Cuts at: 4161 5125 5503 4161 Size: 964 378 6474 Fragments arranged by size: 6474 964 378 FspI TGC'GCA Cuts at: 3177 6322 3177 Size: 3145 4671 HaeII r_GCGC'y Cuts at: 1678 6963 1678 Size: 5285 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2475 2507 3749 3781 6457 Size: 32 135 32 903 32 1242 32 2676 Cuts at: 6457 6489 6531 6563 1373 Size: 32 42 32 2626 Fragments arranged by size: 2676 2626 1242 903 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5160 5406 669 Size: 988 281 3222 246 3079 Fragments arranged by size: 3222 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3449 3588 748 Size: 2701 139 4976 Fragments arranged by size: 4976 2701 139 HpaI GTT'AAC Cuts at: 5406 5406 Size: 7816 KpnI G_GTAC'C Cuts at: 5141 5141 Size: 7816 MfeI C'AATT_G Cuts at: 5415 5415 Size: 7816 MluI A'CGCG_T Cuts at: 7692 7692 Size: 7816 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2132 3503 4805 6930 7114 1305 Size: 827 1371 1302 2125 184 2007 Fragments arranged by size: 2125 2007 1371 1302 827 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5994 Fragments arranged by size: 5994 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2115 4300 5774 6133 6292 518 Size: 648 949 2185 1474 359 159 2042 Fragments arranged by size: 2185 2042 1474 949 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2360 5232 5909 7057 7302 1606 Size: 754 2872 677 1148 245 2120 Fragments arranged by size: 2872 2120 1148 754 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6307 Fragments arranged by size: 6307 957 552 NdeI CA'TA_TG Cuts at: 387 2112 387 Size: 1725 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7816 NotI GC'GGCC_GC Cuts at: 5263 5263 Size: 7816 NsiI A_TGCA'T Cuts at: 2122 2122 Size: 7816 NspI r_CATG'y Cuts at: 1132 1229 5174 6719 1132 Size: 97 3945 1545 2229 Fragments arranged by size: 3945 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7816 PciI A'CATG_T Cuts at: 6715 6715 Size: 7816 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7816 PmeI GTTT'AAAC Cuts at: 5120 5120 Size: 7816 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4493 4525 5885 5917 7398 7430 4493 Size: 32 1360 32 1481 32 4879 Fragments arranged by size: 4879 1481 1360 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7816 PsiI TTA'TAA Cuts at: 5386 5386 Size: 7816 PstI C_TGCA'G Cuts at: 830 3468 5168 830 Size: 2638 1700 3478 Fragments arranged by size: 3478 2638 1700 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7816 PvuII CAG'CTG Cuts at: 2360 2360 Size: 7816 SacI G_AGCT'C Cuts at: 4569 4916 4569 Size: 347 7469 SalI G'TCGA_C Cuts at: 1936 5158 1936 Size: 3222 4594 SapI GCTCTTCn'nnn_ Cuts at: 3567 4119 3567 Size: 552 7264 SbfI CC_TGCA'GG Cuts at: 5168 5168 Size: 7816 ScaI AGT'ACT Cuts at: 4520 6064 7776 4520 Size: 1544 1712 4560 Fragments arranged by size: 4560 1712 1544 SfcI C'TryA_G Cuts at: 826 1045 3439 3464 4654 5164 6299 6980 7171 Size: 219 2394 25 1190 510 1135 681 191 Cuts at: 7171 826 Size: 1471 Fragments arranged by size: 2394 1471 1190 1135 681 510 219 191 25 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7816 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7816 SmaI CCC'GGG Cuts at: 1872 5143 1872 Size: 3271 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2239 3623 5920 6821 7083 820 Size: 197 926 296 1384 2297 901 262 1553 Fragments arranged by size: 2297 1553 1384 926 901 296 262 197 SnaBI TAC'GTA Cuts at: 493 5113 5607 493 Size: 4620 494 2702 Fragments arranged by size: 4620 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7816 SphI G_CATG'C Cuts at: 1132 5174 1132 Size: 4042 3774 SspI AAT'ATT Cuts at: 5 52 4042 4063 5740 5 Size: 47 3990 21 1677 2081 Fragments arranged by size: 3990 2081 1677 47 21 StuI AGG'CCT Cuts at: 1517 1517 Size: 7816 StyI C'CwwG_G Cuts at: 513 1065 2022 2854 3674 5255 513 Size: 552 957 832 820 1581 3074 Fragments arranged by size: 3074 1581 957 832 820 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5843 5860 6013 6198 6643 325 Size: 1255 4263 17 153 185 445 1498 Fragments arranged by size: 4263 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2291 3828 4470 Size: 274 80 33 51 1443 314 1537 642 Cuts at: 4470 4518 4775 4811 6062 7774 96 Size: 48 257 36 1251 1712 138 Fragments arranged by size: 1712 1537 1443 1251 642 314 274 257 138 80 51 48 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2182 2214 4799 4831 7776 7808 2182 Size: 32 2585 32 2945 32 2190 Fragments arranged by size: 2945 2585 2190 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3256 4501 1316 Size: 1940 1245 4631 Fragments arranged by size: 4631 1940 1245 XbaI T'CTAG_A Cuts at: 5021 5152 5021 Size: 131 7685 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3274 3808 3274 Size: 534 7282 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7816 XmaI C'CCGG_G Cuts at: 1870 5141 1870 Size: 3271 4545 XmnI GAAnn'nnTTC Cuts at: 3501 5945 3501 Size: 2444 5372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5624 276 Size: 53 83 186 936 4090 2468 Fragments arranged by size: 4090 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI