Restriction map for
pFN21ASDA0277
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BcgI BciVI BclI BfaI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT14I FauI FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI MaeI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspI
NspV PacI PflMI PinAI PmeI
PshAI Psp1406I PvuI RsrII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 676 CTTAAG C^TTAAG
AflIII 737 ACGTGT A^C[GA][CT]GT
AseI 1056 ATTAAT AT^TAAT
AsnI 1056 ATTAAT AT^TAAT
BanII 1711 GAGCTC G[GA]GC[CT]^C
BbrPI 736 CACGTG CAC^GTG
BbvCI 15 GCTGAGG CCTCAGC(-5/-2)
BfrI 676 CTTAAG C^TTAAG
BglI 1223 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 950 ACCGGA [AT]^CCGG[AT]
BseRI 124 GAGGAG GAGGAG(10/8)
BsgI 1240 GTGCAG GTGCAG(16/14)
BsmFI 223 GGGAC GGGAC(10/14)
Bsp1407I 470 TGTACA T^GTACA
BsrDI 1178 GCAATG GCAATG(2/0)
BsrGI 470 TGTACA T^GTACA
BssSI 1093 CACGAG CACGAG(-5/-1)
BstXI 1313 CCATTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 61 GCGC GCG^C
DraI 513 TTTAAA TTT^AAA
DraII 223 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 838 GAAGAG CTCTTC(1/4)
EcoO109I 223 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1299 GAATTC G^AATTC
EcoT22I 1451 ATGCAT ATGCA^T
HaeII 60 GGCGCT [GA]GCGC^[CT]
HhaI 61 GCGC GCG^C
HinP1I 61 GCGC G^CGC
HincII 1346 GTCGAC GT[CT]^[GA]AC
HindII 1346 GTCGAC GT[CT]^[GA]AC
Ksp632I 838 GAAGAG CTCTTC(1/4)
KspI 56 CCGCGG CCGC^GG
NdeI 1573 CATATG CA^TATG
NsiI 1451 ATGCAT ATGCA^T
PaeR7I 922 CTCGAG C^TCGAG
PmaCI 736 CACGTG CAC^GTG
PmlI 736 CACGTG CAC^GTG
PpuMI 223 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 1056 ATTAAT AT^TAAT
SacI 1711 GAGCTC GAGCT^C
SacII 56 CCGCGG CCGC^GG
SalI 1346 GTCGAC G^TCGAC
SspBI 470 TGTACA T^GTACA
SspI 629 AATATT AAT^ATT
XhoI 922 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 946 GTCTAC GT^[AC][GT]AC
1346 GTCGAC
AccII 57 CGCG CG^CG
422 CGCG
AspI 901 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1585 GACACTGTC
AvaI 922 CTCGAG C^[CT]CG[GA]G
1733 CTCGGG
AvaII 224 GGACC G^G[AT]CC
1185 GGTCC
BalI 857 TGGCCA TGG^CCA
1199 TGGCCA
BbsI 162 GTCTTC GAAGAC(2/6)
331 GTCTTC
BpmI 691 CTCCAG CTGGAG(16/14)
1168 CTCCAG
BpuAI 162 GTCTTC GAAGAC(2/6)
331 GTCTTC
BsaAI 321 CACGTA [CT]AC^GT[GA]
736 CACGTG
BsaI 295 GAGACC GGTCTC(1/5)
1324 GGTCTC
BsmI 602 GAATGC GAATGC(1/-1)
1453 GCATTC
BsoBI 922 CTCGAG C^[CT]CG[GA]G
1733 CTCGGG
BspHI 335 TCATGA T^CATGA
1521 TCATGA
BstEII 482 GGTTACC G^GT[ACGT]ACC
1627 GGTGACC
BstPI 482 GGTTACC G^GT[ACGT]ACC
1627 GGTGACC
BstUI 57 CGCG CG^CG
422 CGCG
Cfr13I 224 GGACC G^G[ACGT]CC
1185 GGTCC
DsaI 56 CCGCGG C^C[GA][CT]GG
1202 CCACGG
EaeI 857 TGGCCA [CT]^GGCC[GA]
1199 TGGCCA
EcoO65I 482 GGTTACC G^GT[ACGT]ACC
1627 GGTGACC
HaeIII 858 GGCC GG^CC
1200 GGCC
MluNI 857 TGGCCA TGG^CCA
1199 TGGCCA
MscI 857 TGGCCA TGG^CCA
1199 TGGCCA
MvnI 57 CGCG CG^CG
422 CGCG
PflFI 901 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1585 GACACTGTC
PstI 1218 CTGCAG CTGCA^G
1608 CTGCAG
PvuII 1225 CAGCTG CAG^CTG
1243 CAGCTG
RcaI 335 TCATGA T^CATGA
1521 TCATGA
Sau96I 224 GGACC G^G[ACGT]CC
1185 GGTCC
SmlI 676 CTTAAG C^T[CT][GA]AG
922 CTCGAG
Tth111I 901 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1585 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HapII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI