Restriction map for
pFN21ASDA0277
(Linear) (Six-base) MAPSORT of: KIAA0277.seq Check: 2983 from: 1 to: 1740
KIAA0277.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 947 1347 1740
Size: 947 400 393
Fragments arranged by size:
947 400 393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 874 1740
Size: 509 365 866
Fragments arranged by size:
866 509 365
AflII C'TTAA_G
Cuts at: 0 676 1740
Size: 676 1064
AflIII A'CryG_T
Cuts at: 0 737 1740
Size: 737 1003
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 395 1255 1740
Size: 395 860 485
Fragments arranged by size:
860 485 395
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 138 170 1740
Size: 138 32 1570
Fragments arranged by size:
1570 138 32
AlwNI CAG_nnn'CTG
Cuts at: 0 363 1588 1704 1740
Size: 363 1225 116 36
Fragments arranged by size:
1225 363 116 36
ApoI r'AATT_y
Cuts at: 0 583 725 1062 1255 1299 1740
Size: 583 142 337 193 44 441
Fragments arranged by size:
583 441 337 193 142 44
AseI AT'TA_AT
Cuts at: 0 1057 1740
Size: 1057 683
AvaI C'yCGr_G
Cuts at: 0 922 1733 1740
Size: 922 811 7
Fragments arranged by size:
922 811 7
BanII G_rGCy'C
Cuts at: 0 1715 1740
Size: 1715 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 324 1740
Size: 155 169 1416
Fragments arranged by size:
1416 169 155
BbvCI CC'TCA_GC
Cuts at: 0 16 1740
Size: 16 1724
BfrBI ATG'CAT
Cuts at: 0 1453 1740
Size: 1453 287
BglI GCCn_nnn'nGGC
Cuts at: 0 1229 1740
Size: 1229 511
BmrI ACTGGGnnnn_n'
Cuts at: 0 450 1740
Size: 450 1290
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 195 426 1740
Size: 195 231 1314
Fragments arranged by size:
1314 231 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1153 1740
Size: 676 477 587
Fragments arranged by size:
676 587 477
Bpu10I CC'TnA_GC
Cuts at: 0 16 1740
Size: 16 1724
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 1330 1740
Size: 289 1041 410
Fragments arranged by size:
1041 410 289
BsaAI yAC'GTr
Cuts at: 0 323 738 1740
Size: 323 415 1002
Fragments arranged by size:
1002 415 323
BsaWI w'CCGG_w
Cuts at: 0 950 1740
Size: 950 790
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 138 168 1740
Size: 138 30 1572
Fragments arranged by size:
1572 138 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 1740
Size: 139 1601
BseYI C'CCAG_C
Cuts at: 0 1227 1740
Size: 1227 513
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1261 1740
Size: 1261 479
BsiHKAI G_wGCw'C
Cuts at: 0 248 755 1715 1740
Size: 248 507 960 25
Fragments arranged by size:
960 507 248 25
BsmI GAATG_Cn'
Cuts at: 0 608 1453 1740
Size: 608 845 287
Fragments arranged by size:
845 608 287
Bsp1286I G_dGCh'C
Cuts at: 0 248 755 1715 1740
Size: 248 507 960 25
Fragments arranged by size:
960 507 248 25
BspHI T'CATG_A
Cuts at: 0 335 1521 1740
Size: 335 1186 219
Fragments arranged by size:
1186 335 219
BsrDI GCAATG_nn'
Cuts at: 0 1185 1740
Size: 1185 555
BsrGI T'GTAC_A
Cuts at: 0 470 1740
Size: 470 1270
BssSI C'ACGA_G
Cuts at: 0 1093 1740
Size: 1093 647
BstEII G'GTnAC_C
Cuts at: 0 482 1627 1740
Size: 482 1145 113
Fragments arranged by size:
1145 482 113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1320 1740
Size: 1320 420
BstYI r'GATC_y
Cuts at: 0 800 824 843 1041 1419 1740
Size: 800 24 19 198 378 321
Fragments arranged by size:
800 378 321 198 24 19
BtgI C'CryG_G
Cuts at: 0 56 1202 1740
Size: 56 1146 538
Fragments arranged by size:
1146 538 56
BtsI GCAGTG_nn'
Cuts at: 0 1145 1275 1331 1605 1740
Size: 1145 130 56 274 135
Fragments arranged by size:
1145 274 135 130 56
DraI TTT'AAA
Cuts at: 0 515 1740
Size: 515 1225
EaeI y'GGCC_r
Cuts at: 0 857 1199 1740
Size: 857 342 541
Fragments arranged by size:
857 541 342
EarI CTCTTCn'nnn_
Cuts at: 0 833 1740
Size: 833 907
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 42 979 1740
Size: 42 937 761
Fragments arranged by size:
937 761 42
EcoICRI GAG'CTC
Cuts at: 0 1713 1740
Size: 1713 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 676 874 1153 1740
Size: 509 167 198 279 587
Fragments arranged by size:
587 509 279 198 167
EcoO109I rG'GnC_Cy
Cuts at: 0 224 1740
Size: 224 1516
EcoRI G'AATT_C
Cuts at: 0 1299 1740
Size: 1299 441
HaeII r_GCGC'y
Cuts at: 0 64 1740
Size: 64 1676
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1645 1677 1716 1740
Size: 1645 32 39 24
Fragments arranged by size:
1645 39 32 24
HincII GTy'rAC
Cuts at: 0 1348 1740
Size: 1348 392
MscI TGG'CCA
Cuts at: 0 859 1201 1740
Size: 859 342 539
Fragments arranged by size:
859 539 342
MslI CAynn'nnrTG
Cuts at: 0 758 1318 1679 1740
Size: 758 560 361 61
Fragments arranged by size:
758 560 361 61
MspA1I CmG'CkG
Cuts at: 0 58 1227 1245 1740
Size: 58 1169 18 495
Fragments arranged by size:
1169 495 58 18
NdeI CA'TA_TG
Cuts at: 0 1574 1740
Size: 1574 166
NsiI A_TGCA'T
Cuts at: 0 1455 1740
Size: 1455 285
PasI CC'CwG_GG
Cuts at: 0 1375 1740
Size: 1375 365
PfoI T'CCnGG_A
Cuts at: 0 220 278 302 1740
Size: 220 58 24 1438
Fragments arranged by size:
1438 220 58 24
PmlI CAC'GTG
Cuts at: 0 738 1740
Size: 738 1002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 138 170 1740
Size: 138 32 1570
Fragments arranged by size:
1570 138 32
PpuMI rG'GwC_Cy
Cuts at: 0 224 1740
Size: 224 1516
PspXI vC'TCGA_Gb
Cuts at: 0 922 1740
Size: 922 818
PstI C_TGCA'G
Cuts at: 0 1222 1612 1740
Size: 1222 390 128
Fragments arranged by size:
1222 390 128
PvuII CAG'CTG
Cuts at: 0 1227 1245 1740
Size: 1227 18 495
Fragments arranged by size:
1227 495 18
SacI G_AGCT'C
Cuts at: 0 1715 1740
Size: 1715 25
SacII CC_GC'GG
Cuts at: 0 59 1740
Size: 59 1681
SalI G'TCGA_C
Cuts at: 0 1346 1740
Size: 1346 394
SanDI GG'GwC_CC
Cuts at: 0 224 1740
Size: 224 1516
SfcI C'TryA_G
Cuts at: 0 620 1218 1443 1608 1740
Size: 620 598 225 165 132
Fragments arranged by size:
620 598 225 165 132
SmlI C'TyrA_G
Cuts at: 0 676 922 1740
Size: 676 246 818
Fragments arranged by size:
818 676 246
SspI AAT'ATT
Cuts at: 0 631 1740
Size: 631 1109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 86 1185 1740
Size: 86 1099 555
Fragments arranged by size:
1099 555 86
TatI w'GTAC_w
Cuts at: 0 470 534 1740
Size: 470 64 1206
Fragments arranged by size:
1206 470 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1463 1495 1740
Size: 1463 32 245
Fragments arranged by size:
1463 245 32
Tth111I GACn'n_nGTC
Cuts at: 0 904 1588 1740
Size: 904 684 152
Fragments arranged by size:
904 684 152
XhoI C'TCGA_G
Cuts at: 0 922 1740
Size: 922 818
XmnI GAAnn'nnTTC
Cuts at: 0 1255 1303 1393 1740
Size: 1255 48 90 347
Fragments arranged by size:
1255 347 90 48
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI AloI AlwNI ApoI
AseI AvaI BanII BbsI BbvCI BfrBI BglI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI
BssSI BstEII BstXI BstYI BtgI BtsI DraI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I
HincII MscI MslI MspA1I NdeI NsiI PasI PfoI
PmlI PpiI PpuMI PspXI PstI PvuII SacI SacII
SalI SanDI SfcI SmlI SspI TaqII TatI TstI
Tth111I XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanI
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmtI BpuEI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BssHII BstAPI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EagI EcoNI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI PacI PciI PflMI PmeI PshAI
PsiI PspOMI PsrI PvuI RsrII SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI StyI SwaI XbaI XcmI XmaI
ZraI