Restriction map for
pFN21ASDA0253
(Linear) (Six-base) MAPSORT of: KIAA0253.seq Check: 3718 from: 1 to: 2127
KIAA0253.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 694 2127
Size: 694 1433
AccI GT'mk_AC
Cuts at: 0 1031 2127
Size: 1031 1096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 947 1439 1503 2127
Size: 702 245 492 64 624
Fragments arranged by size:
702 624 492 245 64
AflII C'TTAA_G
Cuts at: 0 1107 2127
Size: 1107 1020
AhdI GACnn_n'nnGTC
Cuts at: 0 750 1626 2127
Size: 750 876 501
Fragments arranged by size:
876 750 501
AleI CACnn'nnGTG
Cuts at: 0 1334 2127
Size: 1334 793
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 105 2127
Size: 105 2022
AlwNI CAG_nnn'CTG
Cuts at: 0 883 1144 1447 2116 2127
Size: 883 261 303 669 11
Fragments arranged by size:
883 669 303 261 11
ApaI G_GGCC'C
Cuts at: 0 1822 2127
Size: 1822 305
ApoI r'AATT_y
Cuts at: 0 492 1716 2127
Size: 492 1224 411
Fragments arranged by size:
1224 492 411
AseI AT'TA_AT
Cuts at: 0 1388 2127
Size: 1388 739
AvaI C'yCGr_G
Cuts at: 0 33 1020 1745 2102 2127
Size: 33 987 725 357 25
Fragments arranged by size:
987 725 357 33 25
BamHI G'GATC_C
Cuts at: 0 1761 2127
Size: 1761 366
BanI G'GyrC_C
Cuts at: 0 942 1336 2127
Size: 942 394 791
Fragments arranged by size:
942 791 394
BanII G_rGCy'C
Cuts at: 0 1299 1417 1822 1924 2127
Size: 1299 118 405 102 203
Fragments arranged by size:
1299 405 203 118 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 182 527 975 2127
Size: 182 345 448 1152
Fragments arranged by size:
1152 448 345 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1736 1770 2127
Size: 1736 34 357
Fragments arranged by size:
1736 357 34
BclI T'GATC_A
Cuts at: 0 2009 2127
Size: 2009 118
BfrBI ATG'CAT
Cuts at: 0 663 2127
Size: 663 1464
BglI GCCn_nnn'nGGC
Cuts at: 0 880 2127
Size: 880 1247
BlpI GC'TnA_GC
Cuts at: 0 615 1411 2127
Size: 615 796 716
Fragments arranged by size:
796 716 615
Bme1580I G_kGCm'C
Cuts at: 0 421 1822 1974 2127
Size: 421 1401 152 153
Fragments arranged by size:
1401 421 153 152
BmgBI CAC'GTC
Cuts at: 0 1486 2127
Size: 1486 641
BmrI ACTGGGnnnn_n'
Cuts at: 0 377 806 2127
Size: 377 429 1321
Fragments arranged by size:
1321 429 377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1656 2127
Size: 313 1343 471
Fragments arranged by size:
1343 471 313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2023 2127
Size: 2023 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 52 2127
Size: 52 2075
BsaAI yAC'GTr
Cuts at: 0 222 2127
Size: 222 1905
BsaBI GATnn'nnATC
Cuts at: 0 121 2127
Size: 121 2006
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 2127
Size: 250 1877
BseYI C'CCAG_C
Cuts at: 0 498 911 1092 1256 1479 2127
Size: 498 413 181 164 223 648
Fragments arranged by size:
648 498 413 223 181 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 1081 1856 2127
Size: 111 970 775 271
Fragments arranged by size:
970 775 271 111
BsiHKAI G_wGCw'C
Cuts at: 0 1299 1491 1924 2127
Size: 1299 192 433 203
Fragments arranged by size:
1299 433 203 192
BsmI GAATG_Cn'
Cuts at: 0 1827 2127
Size: 1827 300
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1831 2127
Size: 1831 296
Bsp1286I G_dGCh'C
Cuts at: 0 421 1299 1417 1491 1822 1924 1974 2127
Size: 421 878 118 74 331 102 50 153
Fragments arranged by size:
878 421 331 153 118 102 74 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 73 694 2127
Size: 73 621 1433
Fragments arranged by size:
1433 621 73
BsrDI GCAATG_nn'
Cuts at: 0 510 975 2093 2127
Size: 510 465 1118 34
Fragments arranged by size:
1118 510 465 34
BsrGI T'GTAC_A
Cuts at: 0 411 1698 2127
Size: 411 1287 429
Fragments arranged by size:
1287 429 411
BstEII G'GTnAC_C
Cuts at: 0 1551 2127
Size: 1551 576
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 1458 1479 2127
Size: 843 615 21 648
Fragments arranged by size:
843 648 615 21
BstYI r'GATC_y
Cuts at: 0 1140 1185 1761 1791 2127
Size: 1140 45 576 30 336
Fragments arranged by size:
1140 576 336 45 30
Bsu36I CC'TnA_GG
Cuts at: 0 393 1237 1612 1624 2127
Size: 393 844 375 12 503
Fragments arranged by size:
844 503 393 375 12
BtgI C'CryG_G
Cuts at: 0 1472 2127
Size: 1472 655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1647 1971 2127
Size: 1647 324 156
Fragments arranged by size:
1647 324 156
BtsI GCAGTG_nn'
Cuts at: 0 371 471 586 677 899 1373 1445 1759
Size: 371 100 115 91 222 474 72 314
Cuts at: 1759 2127
Size: 368
Fragments arranged by size:
474 371 368 314 222 115 100 91
72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1677 1712 2127
Size: 1677 35 415
Fragments arranged by size:
1677 415 35
DraIII CAC_nnn'GTG
Cuts at: 0 637 2127
Size: 637 1490
EaeI y'GGCC_r
Cuts at: 0 1193 1469 2127
Size: 1193 276 658
Fragments arranged by size:
1193 658 276
EarI CTCTTCn'nnn_
Cuts at: 0 1201 1415 1655 2127
Size: 1201 214 240 472
Fragments arranged by size:
1201 472 240 214
EcoICRI GAG'CTC
Cuts at: 0 1297 1922 2127
Size: 1297 625 205
Fragments arranged by size:
1297 625 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 702 947 1439 1503 1656 2127
Size: 313 389 245 492 64 153 471
Fragments arranged by size:
492 471 389 313 245 153 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 392 1615 2127
Size: 392 1223 512
Fragments arranged by size:
1223 512 392
EcoO109I rG'GnC_Cy
Cuts at: 0 240 1425 1455 1620 1628 1818 1819 1886
Size: 240 1185 30 165 8 190 1 67
Cuts at: 1886 2127
Size: 241
Fragments arranged by size:
1185 241 240 190 165 67 30 8
1
EcoRI G'AATT_C
Cuts at: 0 492 2127
Size: 492 1635
EcoRV GAT'ATC
Cuts at: 0 1965 2127
Size: 1965 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 1233 1265 1612 1644 2127
Size: 736 32 465 32 347 32 483
Fragments arranged by size:
736 483 465 347 32 32 32
HincII GTy'rAC
Cuts at: 0 1077 1737 2127
Size: 1077 660 390
Fragments arranged by size:
1077 660 390
HindIII A'AGCT_T
Cuts at: 0 929 2127
Size: 929 1198
MscI TGG'CCA
Cuts at: 0 1195 1471 2127
Size: 1195 276 656
Fragments arranged by size:
1195 656 276
MslI CAynn'nnrTG
Cuts at: 0 662 688 1334 1390 1832 2127
Size: 662 26 646 56 442 295
Fragments arranged by size:
662 646 442 295 56 26
MspA1I CmG'CkG
Cuts at: 0 915 1955 2127
Size: 915 1040 172
Fragments arranged by size:
1040 915 172
NsiI A_TGCA'T
Cuts at: 0 665 2127
Size: 665 1462
NspI r_CATG'y
Cuts at: 0 663 667 693 777 2127
Size: 663 4 26 84 1350
Fragments arranged by size:
1350 663 84 26 4
PasI CC'CwG_GG
Cuts at: 0 877 2127
Size: 877 1250
PflMI CCAn_nnn'nTGG
Cuts at: 0 427 2127
Size: 427 1700
PpuMI rG'GwC_Cy
Cuts at: 0 240 1425 1620 1628 2127
Size: 240 1185 195 8 499
Fragments arranged by size:
1185 499 240 195 8
PspOMI G'GGCC_C
Cuts at: 0 1818 2127
Size: 1818 309
PspXI vC'TCGA_Gb
Cuts at: 0 1020 2127
Size: 1020 1107
PstI C_TGCA'G
Cuts at: 0 478 1283 2127
Size: 478 805 844
Fragments arranged by size:
844 805 478
PvuII CAG'CTG
Cuts at: 0 915 2127
Size: 915 1212
RsrII CG'GwC_CG
Cuts at: 0 1842 2127
Size: 1842 285
SacI G_AGCT'C
Cuts at: 0 1299 1924 2127
Size: 1299 625 203
Fragments arranged by size:
1299 625 203
ScaI AGT'ACT
Cuts at: 0 1804 2127
Size: 1804 323
SexAI A'CCwGG_T
Cuts at: 0 1175 2127
Size: 1175 952
SfcI C'TryA_G
Cuts at: 0 244 474 1279 2127
Size: 244 230 805 848
Fragments arranged by size:
848 805 244 230
SmaI CCC'GGG
Cuts at: 0 35 2104 2127
Size: 35 2069 23
Fragments arranged by size:
2069 35 23
SmlI C'TyrA_G
Cuts at: 0 1020 1107 2002 2127
Size: 1020 87 895 125
Fragments arranged by size:
1020 895 125 87
SphI G_CATG'C
Cuts at: 0 667 693 777 2127
Size: 667 26 84 1350
Fragments arranged by size:
1350 667 84 26
SspI AAT'ATT
Cuts at: 0 1354 2127
Size: 1354 773
StyI C'CwwG_G
Cuts at: 0 1451 1709 2127
Size: 1451 258 418
Fragments arranged by size:
1451 418 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 925 1627 1859 2127
Size: 925 702 232 268
Fragments arranged by size:
925 702 268 232
TatI w'GTAC_w
Cuts at: 0 411 1698 1802 2127
Size: 411 1287 104 325
Fragments arranged by size:
1287 411 325 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 89 121 232 264 611 643 2127
Size: 89 32 111 32 347 32 1484
Fragments arranged by size:
1484 347 111 89 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 2127
Size: 318 1809
XhoI C'TCGA_G
Cuts at: 0 1020 2127
Size: 1020 1107
XmaI C'CCGG_G
Cuts at: 0 33 2102 2127
Size: 33 2069 25
Fragments arranged by size:
2069 33 25
Enzymes that do cut:
AarI AccI AcuI AflII AhdI AleI AlfI AlwNI
ApaI ApoI AseI AvaI BamHI BanI BanII BbsI
BcgI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaAI BsaBI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
Hin4I HincII HindIII MscI MslI MspA1I NsiI NspI
PasI PflMI PpuMI PspOMI PspXI PstI PvuII RsrII
SacI ScaI SexAI SfcI SmaI SmlI SphI SspI
StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AloI ApaLI
AscI AsiSI AvrII BaeI BbeI BbvCI BciVI BglII
BmtI BplI Bpu10I BsaHI BsaWI BsaXI BsiEI BsiWI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstZ17I ClaI DraI DrdI EagI EciI FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PsrI PvuI SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI StuI
SwaI Tth111I XbaI XmnI ZraI