Restriction map for
pFN21ASDA0250
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspI
AvaI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DraIII EaeI EagI EclXI Eco47III
Eco52I EcoRV FbaI FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI NarI NciI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 2124 GTCTAC GT^[AC][GT]AC
AccII 15 CGCG CG^CG
AhdI 1121 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2272 CCTCAGG CC^T[ACGT]AGG
AseI 1473 ATTAAT AT^TAAT
AsnI 1473 ATTAAT AT^TAAT
Asp700I 1191 GAATAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1121 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2629 GAGCTC G[AT]GC[AT]^C
BamHI 2577 GGATCC G^GATCC
BglII 2503 AGATCT A^GATCT
BsaWI 2665 ACCGGA [AT]^CCGG[AT]
BsiHKAI 2629 GAGCTC G[AT]GC[AT]^C
BsmI 2591 GAATGC GAATGC(1/-1)
Bsp1407I 328 TGTACA T^GTACA
BspMI 2139 GCAGGT ACCTGC(4/8)
BsrFI 2187 GCCGGC [GA]^CCGG[CT]
BsrGI 328 TGTACA T^GTACA
BssHII 14 GCGCGC G^CGCGC
BssSI 1658 CACGAG CACGAG(-5/-1)
BstUI 15 CGCG CG^CG
Bsu36I 2272 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2187 GCCGGC [GA]^CCGG[CT]
Eam1105I 1121 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2272 CCTCAGG CC^T[ACGT]AGG
EcoRI 2464 GAATTC G^AATTC
EcoT22I 3184 ATGCAT ATGCA^T
HaeII 409 AGCGCC [GA]GCGC^[CT]
HgaI 399 GACGC GACGC(5/10)
HindIII 860 AAGCTT A^AGCTT
MaeII 3052 ACGT A^CGT
MfeI 373 CAATTG C^AATTG
MunI 373 CAATTG C^AATTG
MvnI 15 CGCG CG^CG
NaeI 2187 GCCGGC GCC^GGC
NdeI 1865 CATATG CA^TATG
NgoMIV 2187 GCCGGC G^CCGGC
NsiI 3184 ATGCAT ATGCA^T
NspI 528 GCATGC [GA]CATG^[CT]
PflMI 1100 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1473 ATTAAT AT^TAAT
SacI 2629 GAGCTC GAGCT^C
SexAI 1979 ACCAGGT A^CC[AT]GGT
SphI 528 GCATGC GCATG^C
SspBI 328 TGTACA T^GTACA
StuI 752 AGGCCT AGG^CCT
Van91I 1100 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2394 TCTAGA T^CTAGA
XmnI 1191 GAATAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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BanI 3097 GGCACC G^G[CT][GA]CC
3253 GGCACC
BbsI 1483 GAAGAC GAAGAC(2/6)
366 GTCTTC
BlpI 535 GCTCAGC GC^T[ACGT]AGC
2107 GCTCAGC
Bpu1102I 535 GCTCAGC GC^T[ACGT]AGC
2107 GCTCAGC
BpuAI 1483 GAAGAC GAAGAC(2/6)
366 GTCTTC
BstAPI 99 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1019 GCATCCTGTGC
CelII 535 GCTCAGC GC^T[ACGT]AGC
2107 GCTCAGC
DrdI 1731 GACTCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2811 GACTCCGTGGTC
EcoNI 24 CCTCCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
518 CCTACTATAGG
FauI 1878 CCCGC CCCGC(4/6)
2205 CCCGC
SmlI 1221 CTCAAG C^T[CT][GA]AG
1778 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanII BbvI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoO65I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII