Restriction map for
pFN21ASDA0248
##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AspI
AvrII BbrPI BciVI BlnI BsaAI
BsiWI BsmBI BspDI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FseI HpaI KpnI KspI
MfeI MluI MunI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 4670 GGCGCC G[GA]^CG[CT]C
AflII 4168 CTTAAG C^TTAAG
AflIII 2621 ACATGT A^C[GA][CT]GT
Alw44I 1077 GTGCAC G^TGCAC
ApaLI 1077 GTGCAC G^TGCAC
AviII 3671 TGCGCA TGC^GCA
BbeI 4670 GGCGCC GGCGC^C
BcgI 3525 GCAGACTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4168 CTTAAG C^TTAAG
BglI 4085 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3176 AGATCT A^GATCT
BsaHI 4670 GGCGCC G[GA]^CG[CT]C
BsiEI 1254 CGACCG CG[GA][CT]^CG
BspHI 3401 TCATGA T^CATGA
BspLU11I 2621 ACATGT A^CATGT
BsrBI 3589 GAGCGG CCGCTC(-3/-3)
BsrFI 4379 GCCGGC [GA]^CCGG[CT]
BstEII 584 GGTTACC G^GT[ACGT]ACC
BstPI 584 GGTTACC G^GT[ACGT]ACC
Cfr10I 4379 GCCGGC [GA]^CCGG[CT]
DraIII 3251 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3955 GACTCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 584 GGTTACC G^GT[ACGT]ACC
EcoRI 883 GAATTC G^AATTC
FspI 3671 TGCGCA TGC^GCA
HaeII 4670 GGCGCC [GA]GCGC^[CT]
HgaI 4241 GCGTC GACGC(5/10)
Hin1I 4670 GGCGCC G[GA]^CG[CT]C
HindIII 1501 AAGCTT A^AGCTT
KasI 4670 GGCGCC G^GCGCC
NaeI 4379 GCCGGC GCC^GGC
NarI 4670 GGCGCC GG^CGCC
NgoMIV 4379 GCCGGC G^CCGGC
NheI 1864 GCTAGC G^CTAGC
PshAI 720 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3401 TCATGA T^CATGA
SfoI 4670 GGCGCC GGC^GCC
SmaI 1139 CCCGGG CCC^GGG
SspI 13 AATATT AAT^ATT
XbaI 924 TCTAGA T^CTAGA
XmaCI 1139 CCCGGG C^CCGGG
XmaI 1139 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 556 GTAGAC GT^[AC][GT]AC
3964 GTCTAC
AccII 1213 CGCG CG^CG
4520 CGCG
AhdI 4153 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4981 GACTTACTGTC
AocI 2659 CCTGAGG CC^T[ACGT]AGG
2728 CCTGAGG
AspEI 4153 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4981 GACTTACTGTC
BclI 3356 TGATCA T^GATCA
4106 TGATCA
BsaBI 399 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1488 GATGTACATC
Bsp1407I 1490 TGTACA T^GTACA
4719 TGTACA
BspMI 2981 ACCTGC ACCTGC(4/8)
5451 GCAGGT
BsrDI 4862 CATTGC GCAATG(2/0)
3336 GCAATG
BsrGI 1490 TGTACA T^GTACA
4719 TGTACA
BstAPI 3871 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4506 GCACACCCTGC
BstUI 1213 CGCG CG^CG
4520 CGCG
Bsu36I 2659 CCTGAGG CC^T[ACGT]AGG
2728 CCTGAGG
DraI 147 TTTAAA TTT^AAA
595 TTTAAA
Eam1105I 4153 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4981 GACTTACTGTC
Eco81I 2659 CCTGAGG CC^T[ACGT]AGG
2728 CCTGAGG
FbaI 3356 TGATCA T^GATCA
4106 TGATCA
MamI 399 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1488 GATGTACATC
MvnI 1213 CGCG CG^CG
4520 CGCG
NcoI 3975 CCATGG C^CATGG
5180 CCATGG
NdeI 715 CATATG CA^TATG
3440 CATATG
PpuMI 1015 GGGACCC [GA]G^G[AT]CC[CT]
5168 AGGACCC
SexAI 732 ACCAGGT A^CC[AT]GGT
3719 ACCAGGT
SphI 791 GCATGC GCATG^C
1457 GCATGC
SspBI 1490 TGTACA T^GTACA
4719 TGTACA
StuI 1926 AGGCCT AGG^CCT
2172 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspEI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI