(Circular) (Six-base) MAPSORT of: pFN21ASDA0248.seq Check: 8410 from: 1 to: 10306 pFN21ASDA0248 10306 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7465 7465 Size: 10306 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8116 278 Size: 53 83 186 936 6580 2468 Fragments arranged by size: 6580 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2580 5988 7649 1937 Size: 643 3408 1661 4594 Fragments arranged by size: 4594 3408 1661 643 Acc65I G'GTAC_C Cuts at: 7627 7627 Size: 10306 AclI AA'CG_TT Cuts at: 1563 8433 8806 1563 Size: 6870 373 3063 Fragments arranged by size: 6870 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 2417 3105 4318 4879 5792 7055 8369 Size: 633 152 688 1213 561 913 1263 1314 Cuts at: 8369 9753 10021 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1314 1263 1213 913 688 633 561 268 152 AflII C'TTAA_G Cuts at: 820 1017 6191 820 Size: 197 5174 4935 Fragments arranged by size: 5174 4935 197 AflIII A'CryG_T Cuts at: 1203 4644 9205 10182 1203 Size: 3441 4561 977 1327 Fragments arranged by size: 4561 3441 1327 977 AgeI A'CCGG_T Cuts at: 9137 9137 Size: 10306 AhdI GACnn_n'nnGTC Cuts at: 6181 7009 9035 6181 Size: 828 2026 7452 Fragments arranged by size: 7452 2026 828 AleI CACnn'nnGTG Cuts at: 1166 5577 5799 1166 Size: 4411 222 5673 Fragments arranged by size: 5673 4411 222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4781 6684 1184 Size: 3597 1903 4806 Fragments arranged by size: 4806 3597 1903 AlwNI CAG_nnn'CTG Cuts at: 2366 2864 2990 3068 3347 4183 4348 5683 6715 Size: 498 126 78 279 836 165 1335 1032 Cuts at: 6715 6913 8049 9621 2366 Size: 198 1136 1572 3051 Fragments arranged by size: 3051 1572 1335 1136 1032 836 498 279 198 165 126 78 ApaI G_GGCC'C Cuts at: 4820 5309 5911 6105 6640 4820 Size: 489 602 194 535 8486 Fragments arranged by size: 8486 602 535 489 194 ApaLI G'TGCA_C Cuts at: 3100 8363 9519 3100 Size: 5263 1156 3887 Fragments arranged by size: 5263 3887 1156 ApoI r'AATT_y Cuts at: 1493 2089 2906 4970 7615 7841 7993 10102 1493 Size: 596 817 2064 2645 226 152 2109 1697 Fragments arranged by size: 2645 2109 2064 1697 817 596 226 152 AseI AT'TA_AT Cuts at: 160 1032 8860 160 Size: 872 7828 1606 Fragments arranged by size: 7828 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10306 AvaI C'yCGr_G Cuts at: 1870 1943 3014 3162 3879 5730 6432 7334 7344 Size: 73 1071 148 717 1851 702 902 10 Cuts at: 7344 7631 1870 Size: 287 4545 Fragments arranged by size: 4545 1851 1071 902 717 702 287 148 73 10 BamHI G'GATC_C Cuts at: 2131 2395 5185 7636 8003 2131 Size: 264 2790 2451 367 4434 Fragments arranged by size: 4434 2790 2451 367 264 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2468 3875 4742 6648 6693 Size: 325 217 627 681 1407 867 1906 45 Cuts at: 6693 7627 9082 618 Size: 934 1455 1842 Fragments arranged by size: 1906 1842 1455 1407 934 867 681 627 325 217 45 BanII G_rGCy'C Cuts at: 1391 2205 3243 3636 4820 5143 5309 5424 5760 Size: 814 1038 393 1184 323 166 115 336 Cuts at: 5760 5911 5928 6105 6261 6640 7343 7406 7625 Size: 151 17 177 156 379 703 63 219 Cuts at: 7625 1391 Size: 4072 Fragments arranged by size: 4072 1184 1038 814 703 393 379 336 323 219 177 166 156 151 115 63 17 BbeI G_GCGC'C Cuts at: 6697 6697 Size: 10306 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2940 3215 5780 6892 928 Size: 971 1041 275 2565 1112 4342 Fragments arranged by size: 4342 2565 1112 1041 971 275 BbvCI CC'TCA_GC Cuts at: 2869 4931 7234 2869 Size: 2062 2303 5941 Fragments arranged by size: 5941 2303 2062 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5537 5571 8497 8531 1903 Size: 34 3600 34 2926 34 3678 Fragments arranged by size: 3678 3600 2926 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8198 9408 8198 Size: 1210 9096 BclI T'GATC_A Cuts at: 1548 5379 6129 1548 Size: 3831 750 5725 Fragments arranged by size: 5725 3831 750 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6114 8917 136 Size: 107 122 71 631 605 4442 2803 1525 Fragments arranged by size: 4442 2803 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5199 10301 5199 Size: 5102 5204 BlpI GC'TnA_GC Cuts at: 5252 7400 7434 7723 5252 Size: 2148 34 289 7835 Fragments arranged by size: 7835 2148 289 34 Bme1580I G_kGCm'C Cuts at: 1790 2336 2393 2473 2835 3104 4820 5309 5911 Size: 546 57 80 362 269 1716 489 602 Cuts at: 5911 6105 6640 6653 7548 8367 9523 1790 Size: 194 535 13 895 819 1156 2573 Fragments arranged by size: 2573 1716 1156 895 819 602 546 535 489 362 269 194 80 57 13 BmgBI CAC'GTC Cuts at: 1318 3105 5950 1318 Size: 1787 2845 5674 Fragments arranged by size: 5674 2845 1787 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2456 3052 3349 Size: 416 53 83 399 24 1039 596 297 Cuts at: 3349 5004 5712 5818 5905 8037 8995 442 Size: 1655 708 106 87 2132 958 1753 Fragments arranged by size: 2132 1753 1655 1039 958 708 596 416 399 297 106 87 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3891 1056 Size: 2835 7471 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2739 3509 7184 7552 2739 Size: 770 3675 368 5493 Fragments arranged by size: 5493 3675 770 368 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2949 3014 3608 4034 4448 4595 4658 5384 Size: 1710 65 594 426 414 147 63 726 Cuts at: 5384 5426 5844 5852 6400 6611 6982 8966 1239 Size: 42 418 8 548 211 371 1984 2579 Fragments arranged by size: 2579 1984 1710 726 594 548 426 418 414 371 211 147 65 63 42 8 Bpu10I CC'TnA_GC Cuts at: 2869 4931 5286 5504 5676 5845 7234 2869 Size: 2062 355 218 172 169 1389 5941 Fragments arranged by size: 5941 2062 1389 355 218 172 169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3356 3479 4880 7197 7369 8431 9296 9594 3356 Size: 123 1401 2317 172 1062 865 298 4068 Fragments arranged by size: 4068 2317 1401 1062 865 298 172 123 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5443 5553 6857 8969 882 Size: 617 3944 110 1304 2112 2219 Fragments arranged by size: 3944 2219 2112 1304 617 110 BsaAI yAC'GTr Cuts at: 493 1204 7603 8097 493 Size: 711 6399 494 2702 Fragments arranged by size: 6399 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2426 3515 10300 2426 Size: 1089 6785 2432 Fragments arranged by size: 6785 2432 1089 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6694 8113 8495 9130 Size: 53 83 186 936 5161 1419 382 635 Cuts at: 9130 275 Size: 1451 Fragments arranged by size: 5161 1451 1419 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2517 5655 6276 8739 9137 9411 9558 9969 2517 Size: 3138 621 2463 398 274 147 411 2854 Fragments arranged by size: 3138 2854 2463 621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3089 3119 4269 4299 4631 Size: 30 165 30 1686 30 1150 30 332 Cuts at: 4631 4661 6980 7010 1178 Size: 30 2319 30 4474 Fragments arranged by size: 4474 2319 1686 1150 332 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2836 2902 3110 4190 4592 4700 4877 5250 Size: 1650 66 208 1080 402 108 177 373 Cuts at: 5250 6590 6875 1186 Size: 1340 285 4617 Fragments arranged by size: 4617 1650 1340 1080 402 373 285 208 177 108 66 BseYI C'CCAG_C Cuts at: 3617 3825 6104 7309 7391 9509 10005 3617 Size: 208 2279 1205 82 2118 496 3918 Fragments arranged by size: 3918 2279 2118 1205 496 208 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2609 3620 6521 7101 1163 Size: 573 873 1011 2901 580 4368 Fragments arranged by size: 4368 2901 1011 873 580 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 7756 8517 9545 1540 Size: 275 186 1279 4476 761 1028 2301 Fragments arranged by size: 4476 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2105 2571 3104 3243 3492 3636 4331 5424 5677 Size: 466 533 139 249 144 695 1093 253 Cuts at: 5677 5928 6261 6736 8367 8452 9523 2105 Size: 251 333 475 1631 85 1071 2888 Fragments arranged by size: 2888 1631 1093 1071 695 533 475 466 333 253 251 249 144 139 85 BsmI GAATG_Cn' Cuts at: 3712 4433 4564 7817 7910 3712 Size: 721 131 3253 93 6108 Fragments arranged by size: 6108 3253 721 131 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2205 2336 2393 2473 2571 2835 Size: 399 315 100 131 57 80 98 264 Cuts at: 2835 3104 3243 3492 3636 4331 4820 5143 5309 Size: 269 139 249 144 695 489 323 166 Cuts at: 5309 5424 5677 5760 5911 5928 6105 6261 6640 Size: 115 253 83 151 17 177 156 379 Cuts at: 6640 6653 6736 7343 7406 7548 7625 8367 8452 Size: 13 83 607 63 142 77 742 85 Cuts at: 8452 9523 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 695 607 489 399 379 323 315 269 264 253 249 177 166 156 151 144 142 139 131 115 100 98 85 83 83 80 77 63 57 17 13 BspEI T'CCGG_A Cuts at: 2517 5655 6276 9969 2517 Size: 3138 621 3693 2854 Fragments arranged by size: 3693 3138 2854 621 BspHI T'CATG_A Cuts at: 5424 8195 9925 5424 Size: 2771 1730 5805 Fragments arranged by size: 5805 2771 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5013 7465 7661 844 Size: 4169 2452 196 3489 Fragments arranged by size: 4169 3489 2452 196 BsrBI CCG'CTC Cuts at: 1394 1823 5614 8193 1394 Size: 429 3791 2579 3507 Fragments arranged by size: 3791 3507 2579 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5366 6884 8801 8975 65 Size: 1183 4118 1518 1917 174 1396 Fragments arranged by size: 4118 1917 1518 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6402 8950 9137 1700 Size: 110 4592 2548 187 2869 Fragments arranged by size: 4592 2869 2548 187 110 BsrGI T'GTAC_A Cuts at: 96 3513 6742 96 Size: 3417 3229 3660 Fragments arranged by size: 3660 3417 3229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10306 BssSI C'ACGA_G Cuts at: 8366 9378 8366 Size: 1012 9294 BstAPI GCAn_nnn'nTGC Cuts at: 794 5900 6535 794 Size: 5106 635 4565 Fragments arranged by size: 5106 4565 635 BstBI TT'CG_AA Cuts at: 2007 9110 2007 Size: 7103 3203 BstEII G'GTnAC_C Cuts at: 1184 2607 1184 Size: 1423 8883 BstXI CCAn_nnnn'nTGG Cuts at: 4213 4573 5078 5338 6823 7204 4213 Size: 360 505 260 1485 381 7315 Fragments arranged by size: 7315 1485 505 381 360 260 BstYI r'GATC_y Cuts at: 1972 2131 2317 2395 2520 2717 4087 5185 5199 Size: 159 186 78 125 197 1370 1098 14 Cuts at: 5199 6279 7573 7636 8003 8388 8405 9857 9943 Size: 1080 1294 63 367 385 17 1452 86 Cuts at: 9943 9955 10301 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1370 1294 1098 1080 385 367 346 197 186 159 125 86 78 63 17 14 12 Bsu36I CC'TnA_GG Cuts at: 4683 4752 4683 Size: 69 10237 BtgI C'CryG_G Cuts at: 513 1065 2022 4091 5998 7203 513 Size: 552 957 2069 1907 1205 3616 Fragments arranged by size: 3616 2069 1907 1205 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3927 4497 4950 6699 487 Size: 683 56 2701 570 453 1749 4094 Fragments arranged by size: 4094 2701 1749 683 570 453 56 BtsI GCAGTG_nn' Cuts at: 2880 3004 3793 4012 4993 5625 5825 6640 6920 Size: 124 789 219 981 632 200 815 280 Cuts at: 6920 7469 7822 8616 8636 2880 Size: 549 353 794 20 4550 Fragments arranged by size: 4550 981 815 794 789 632 549 353 280 219 200 124 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10306 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10271 DraI TTT'AAA Cuts at: 2172 2620 7610 2172 Size: 448 4990 4868 Fragments arranged by size: 4990 4868 448 DraIII CAC_nnn'GTG Cuts at: 5279 5279 Size: 10306 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5984 9313 809 Size: 507 4668 3329 1802 Fragments arranged by size: 4668 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5640 6601 7276 Size: 54 1425 50 275 18 3810 961 675 Cuts at: 7276 7554 7753 8642 10271 8 Size: 278 199 889 1629 43 Fragments arranged by size: 3810 1629 1425 961 889 675 278 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7753 1537 Size: 275 5941 4090 Fragments arranged by size: 5941 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2603 2708 3248 4805 5138 5211 5451 5658 Size: 1250 105 540 1557 333 73 240 207 Cuts at: 5658 5825 7175 8236 1353 Size: 167 1350 1061 3423 Fragments arranged by size: 3423 1557 1350 1250 1061 540 333 240 207 167 105 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4358 4906 6321 6587 8893 9267 Size: 1064 138 2769 548 1415 266 2306 374 Cuts at: 9267 9413 387 Size: 146 1280 Fragments arranged by size: 2769 2306 1415 1280 1064 548 374 266 146 138 EcoICRI GAG'CTC Cuts at: 3241 3634 5422 5926 6259 3241 Size: 393 1788 504 333 7288 Fragments arranged by size: 7288 1788 504 393 333 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 2417 2949 3014 3105 3608 4034 Size: 393 633 152 532 65 91 503 426 Cuts at: 4034 4318 4448 4595 4658 4879 5384 5426 5792 Size: 284 130 147 63 221 505 42 366 Cuts at: 5792 5844 5852 6400 6611 6982 7055 8369 8966 Size: 52 8 548 211 371 73 1314 597 Cuts at: 8966 9753 10021 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1314 787 633 597 548 532 505 503 426 393 371 366 284 268 221 211 152 147 130 91 73 65 63 52 42 8 EcoNI CCTnn'n_nnAGG Cuts at: 3380 3677 5093 3380 Size: 297 1416 8593 Fragments arranged by size: 8593 1416 297 EcoO109I rG'GnC_Cy Cuts at: 3039 4816 4817 5305 5907 6101 6116 7192 7314 Size: 1777 1 488 602 194 15 1076 122 Cuts at: 7314 3039 Size: 6031 Fragments arranged by size: 6031 1777 1076 602 488 194 122 15 1 EcoRI G'AATT_C Cuts at: 2906 7615 7993 2906 Size: 4709 378 5219 Fragments arranged by size: 5219 4709 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3727 3727 Size: 10306 FspI TGC'GCA Cuts at: 5696 8812 5696 Size: 3116 7190 HaeII r_GCGC'y Cuts at: 1678 6697 9453 1678 Size: 5019 2756 2531 Fragments arranged by size: 5019 2756 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2262 2294 3089 3116 3121 Size: 32 135 32 690 32 795 27 5 Cuts at: 3121 3148 3382 3414 5210 5242 5652 5659 5684 Size: 27 234 32 1796 32 410 7 25 Cuts at: 5684 5691 5915 5944 5947 5976 6167 6199 6995 Size: 7 224 29 3 29 191 32 796 Cuts at: 6995 7027 7286 7318 8947 8979 9021 9053 1373 Size: 32 259 32 1629 32 42 32 2626 Fragments arranged by size: 2626 1796 1629 796 795 690 410 259 234 224 191 135 42 32 32 32 32 32 32 32 32 32 32 29 29 27 27 25 7 7 5 3 HincII GTy'rAC Cuts at: 669 1657 1938 2290 3728 4027 5244 6049 7597 Size: 988 281 352 1438 299 1217 805 1548 Cuts at: 7597 7650 7896 669 Size: 53 246 3079 Fragments arranged by size: 3079 1548 1438 1217 988 805 352 299 281 246 53 HindIII A'AGCT_T Cuts at: 748 3524 748 Size: 2776 7530 HpaI GTT'AAC Cuts at: 7896 7896 Size: 10306 KasI G'GCGC_C Cuts at: 6693 6693 Size: 10306 KpnI G_GTAC'C Cuts at: 7631 7631 Size: 10306 MfeI C'AATT_G Cuts at: 7905 7905 Size: 10306 MluI A'CGCG_T Cuts at: 10182 10182 Size: 10306 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3693 4293 4427 5496 6000 9420 9604 1305 Size: 2388 600 134 1069 504 3420 184 2007 Fragments arranged by size: 3420 2388 2007 1069 600 504 184 134 MscI TGG'CCA Cuts at: 10 64 1489 1832 5642 6603 7278 7556 10 Size: 54 1425 343 3810 961 675 278 2760 Fragments arranged by size: 3810 2760 1425 961 675 343 278 54 MslI CAynn'nnrTG Cuts at: 518 1166 2698 2828 3542 4211 4740 5076 5577 Size: 648 1532 130 714 669 529 336 501 Cuts at: 5577 5799 5943 6345 7202 8264 8623 8782 518 Size: 222 144 402 857 1062 359 159 2042 Fragments arranged by size: 2042 1532 1062 857 714 669 648 529 501 402 359 336 222 159 144 130 MspA1I CmG'CkG Cuts at: 1606 2593 5212 5404 5864 7064 7253 7722 8399 Size: 987 2619 192 460 1200 189 469 677 Cuts at: 8399 9547 9792 1606 Size: 1148 245 2120 Fragments arranged by size: 2619 2120 1200 1148 987 677 469 460 245 192 189 NaeI GCC'GGC Cuts at: 6404 6404 Size: 10306 NarI GG'CG_CC Cuts at: 6694 6694 Size: 10306 NcoI C'CATG_G Cuts at: 513 1065 2022 5998 7203 513 Size: 552 957 3976 1205 3616 Fragments arranged by size: 3976 3616 1205 957 552 NdeI CA'TA_TG Cuts at: 387 2739 5464 387 Size: 2352 2725 5229 Fragments arranged by size: 5229 2725 2352 NgoMIV G'CCGG_C Cuts at: 6402 6402 Size: 10306 NheI G'CTAG_C Cuts at: 1052 3887 1052 Size: 2835 7471 NotI GC'GGCC_GC Cuts at: 7753 7753 Size: 10306 NspI r_CATG'y Cuts at: 1132 1229 2818 3484 4648 6428 6988 7045 7664 Size: 97 1589 666 1164 1780 560 57 619 Cuts at: 7664 9209 1132 Size: 1545 2229 Fragments arranged by size: 2229 1780 1589 1545 1164 666 619 560 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10306 PciI A'CATG_T Cuts at: 4644 9205 4644 Size: 4561 5745 PflMI CCAn_nnn'nTGG Cuts at: 1108 3145 3989 4812 1108 Size: 2037 844 823 6602 Fragments arranged by size: 6602 2037 844 823 PfoI T'CCnGG_A Cuts at: 3030 4635 7188 3030 Size: 1605 2553 6148 Fragments arranged by size: 6148 2553 1605 PmeI GTTT'AAAC Cuts at: 7610 7610 Size: 10306 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8375 8407 9888 9920 8375 Size: 32 1481 32 8761 Fragments arranged by size: 8761 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3039 7192 3039 Size: 4153 6153 PshAI GACnn'nnGTC Cuts at: 1613 2747 1613 Size: 1134 9172 PsiI TTA'TAA Cuts at: 7876 7876 Size: 10306 PspOMI G'GGCC_C Cuts at: 4816 5305 5907 6101 6636 4816 Size: 489 602 194 535 8486 Fragments arranged by size: 8486 602 535 489 194 PstI C_TGCA'G Cuts at: 830 5130 5874 6663 6729 6844 6909 7455 7476 Size: 4300 744 789 66 115 65 546 21 Cuts at: 7476 7658 830 Size: 182 3478 Fragments arranged by size: 4300 3478 789 744 546 182 115 66 65 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10306 PvuII CAG'CTG Cuts at: 2593 5404 5864 2593 Size: 2811 460 7035 Fragments arranged by size: 7035 2811 460 SacI G_AGCT'C Cuts at: 3243 3636 5424 5928 6261 3243 Size: 393 1788 504 333 7288 Fragments arranged by size: 7288 1788 504 393 333 SalI G'TCGA_C Cuts at: 1936 7648 1936 Size: 5712 4594 SanDI GG'GwC_CC Cuts at: 3039 3039 Size: 10306 SapI GCTCTTCn'nnn_ Cuts at: 2603 3248 5138 2603 Size: 645 1890 7771 Fragments arranged by size: 7771 1890 645 SbfI CC_TGCA'GG Cuts at: 7658 7658 Size: 10306 ScaI AGT'ACT Cuts at: 8554 10266 8554 Size: 1712 8594 SexAI A'CCwGG_T Cuts at: 2755 5742 2755 Size: 2987 7319 SfcI C'TryA_G Cuts at: 826 1045 2889 2982 3348 3924 5126 5207 5414 Size: 219 1844 93 366 576 1202 81 207 Cuts at: 5414 5870 6559 6659 6725 6840 6905 7451 7472 Size: 456 689 100 66 115 65 546 21 Cuts at: 7472 7654 8789 9470 9661 826 Size: 182 1135 681 191 1471 Fragments arranged by size: 1844 1471 1202 1135 689 681 576 546 456 366 219 207 191 182 115 100 93 81 66 65 21 SfoI GGC'GCC Cuts at: 6695 6695 Size: 10306 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10306 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10306 SmaI CCC'GGG Cuts at: 1872 3164 7633 1872 Size: 1292 4469 4545 Fragments arranged by size: 4545 4469 1292 SmlI C'TyrA_G Cuts at: 820 1017 1943 3335 3494 4859 6191 7212 7384 Size: 197 926 1392 159 1365 1332 1021 172 Cuts at: 7384 8410 9311 9573 820 Size: 1026 901 262 1553 Fragments arranged by size: 1553 1392 1365 1332 1026 1021 926 901 262 197 172 159 SnaBI TAC'GTA Cuts at: 493 7603 8097 493 Size: 7110 494 2702 Fragments arranged by size: 7110 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 10306 SphI G_CATG'C Cuts at: 1132 2818 3484 7664 1132 Size: 1686 666 4180 3774 Fragments arranged by size: 4180 3774 1686 666 SspI AAT'ATT Cuts at: 5 52 2038 8230 5 Size: 47 1986 6192 2081 Fragments arranged by size: 6192 2081 1986 47 StuI AGG'CCT Cuts at: 1517 3951 4197 1517 Size: 2434 246 7626 Fragments arranged by size: 7626 2434 246 StyI C'CwwG_G Cuts at: 513 1065 2022 2928 5154 5998 7203 7428 7458 Size: 552 957 906 2226 844 1205 225 30 Cuts at: 7458 7745 513 Size: 287 3074 Fragments arranged by size: 3074 2226 1205 957 906 844 552 287 225 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2120 2141 2339 2387 2456 3839 4579 Size: 1255 540 21 198 48 69 1383 740 Cuts at: 4579 6839 8333 8350 8503 8688 9133 325 Size: 2260 1494 17 153 185 445 1498 Fragments arranged by size: 2260 1498 1494 1383 1255 740 540 445 198 185 153 69 48 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3513 6742 6981 Size: 274 80 33 51 1443 1536 3229 239 Cuts at: 6981 8552 10264 96 Size: 1571 1712 138 Fragments arranged by size: 3229 1712 1571 1536 1443 274 239 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3377 3409 4269 4301 4398 4430 6980 7012 7232 Size: 32 860 32 97 32 2550 32 220 Cuts at: 7232 7264 7362 7394 10266 10298 3377 Size: 32 98 32 2872 32 3385 Fragments arranged by size: 3385 2872 2550 860 220 98 97 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10306 XbaI T'CTAG_A Cuts at: 2947 7642 2947 Size: 4695 5611 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 5750 6326 2638 Size: 3112 576 6618 Fragments arranged by size: 6618 3112 576 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10306 XmaI C'CCGG_G Cuts at: 1870 3162 7631 1870 Size: 1292 4469 4545 Fragments arranged by size: 4545 4469 1292 XmnI GAAnn'nnTTC Cuts at: 2235 3710 6365 8435 2235 Size: 1475 2655 2070 4106 Fragments arranged by size: 4106 2655 2070 1475 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8114 276 Size: 53 83 186 936 6580 2468 Fragments arranged by size: 6580 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SrfI SwaI