Restriction map for
pFN21ASDA0248
(Linear) (Six-base) MAPSORT of: KIAA0248.seq Check: 5158 from: 1 to: 5577
KIAA0248.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5442 5577
Size: 5442 135
AccI GT'mk_AC
Cuts at: 0 557 3965 5577
Size: 557 3408 1612
Fragments arranged by size:
3408 1612 557
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 394 1082 2295 2856 3769 5032 5577
Size: 242 152 688 1213 561 913 1263 545
Fragments arranged by size:
1263 1213 913 688 561 545 242 152
AflII C'TTAA_G
Cuts at: 0 4168 5577
Size: 4168 1409
AflIII A'CryG_T
Cuts at: 0 2621 5577
Size: 2621 2956
AhdI GACnn_n'nnGTC
Cuts at: 0 4158 4986 5577
Size: 4158 828 591
Fragments arranged by size:
4158 828 591
AleI CACnn'nnGTG
Cuts at: 0 3554 3776 5577
Size: 3554 222 1801
Fragments arranged by size:
3554 1801 222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2758 4661 5577
Size: 2758 1903 916
Fragments arranged by size:
2758 1903 916
AlwNI CAG_nnn'CTG
Cuts at: 0 343 841 967 1045 1324 2160 2325 3660
Size: 343 498 126 78 279 836 165 1335
Cuts at: 3660 4692 4890 5577
Size: 1032 198 687
Fragments arranged by size:
1335 1032 836 687 498 343 279 198
165 126 78
ApaI G_GGCC'C
Cuts at: 0 2797 3286 3888 4082 4617 5577
Size: 2797 489 602 194 535 960
Fragments arranged by size:
2797 960 602 535 489 194
ApaLI G'TGCA_C
Cuts at: 0 1077 5577
Size: 1077 4500
ApoI r'AATT_y
Cuts at: 0 66 883 2947 5577
Size: 66 817 2064 2630
Fragments arranged by size:
2630 2064 817 66
AvaI C'yCGr_G
Cuts at: 0 991 1139 1856 3707 4409 5311 5321 5577
Size: 991 148 717 1851 702 902 10 256
Fragments arranged by size:
1851 991 902 717 702 256 148 10
BamHI G'GATC_C
Cuts at: 0 108 372 3162 5577
Size: 108 264 2790 2415
Fragments arranged by size:
2790 2415 264 108
BanI G'GyrC_C
Cuts at: 0 445 1852 2719 4625 4670 5577
Size: 445 1407 867 1906 45 907
Fragments arranged by size:
1906 1407 907 867 445 45
BanII G_rGCy'C
Cuts at: 0 182 1220 1613 2797 3120 3286 3401 3737
Size: 182 1038 393 1184 323 166 115 336
Cuts at: 3737 3888 3905 4082 4238 4617 5320 5383 5577
Size: 151 17 177 156 379 703 63 194
Fragments arranged by size:
1184 1038 703 393 379 336 323 194
182 177 166 156 151 115 63 17
BbeI G_GCGC'C
Cuts at: 0 4674 5577
Size: 4674 903
BbsI GAAGACnn'nnnn_
Cuts at: 0 917 1192 3757 4869 5577
Size: 917 275 2565 1112 708
Fragments arranged by size:
2565 1112 917 708 275
BbvCI CC'TCA_GC
Cuts at: 0 846 2908 5211 5577
Size: 846 2062 2303 366
Fragments arranged by size:
2303 2062 846 366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3514 3548 5577
Size: 3514 34 2029
Fragments arranged by size:
3514 2029 34
BclI T'GATC_A
Cuts at: 0 3356 4106 5577
Size: 3356 750 1471
Fragments arranged by size:
3356 1471 750
BglI GCCn_nnn'nGGC
Cuts at: 0 4091 5577
Size: 4091 1486
BglII A'GATC_T
Cuts at: 0 3176 5577
Size: 3176 2401
BlpI GC'TnA_GC
Cuts at: 0 3229 5377 5411 5577
Size: 3229 2148 34 166
Fragments arranged by size:
3229 2148 166 34
Bme1580I G_kGCm'C
Cuts at: 0 313 370 450 812 1081 2797 3286 3888
Size: 313 57 80 362 269 1716 489 602
Cuts at: 3888 4082 4617 4630 5525 5577
Size: 194 535 13 895 52
Fragments arranged by size:
1716 895 602 535 489 362 313 269
194 80 57 52 13
BmgBI CAC'GTC
Cuts at: 0 1082 3927 5577
Size: 1082 2845 1650
Fragments arranged by size:
2845 1650 1082
BmrI ACTGGGnnnn_n'
Cuts at: 0 433 1029 1326 2981 3689 3795 3882 5577
Size: 433 596 297 1655 708 106 87 1695
Fragments arranged by size:
1695 1655 708 596 433 297 106 87
BmtI G_CTAG'C
Cuts at: 0 1868 5577
Size: 1868 3709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 716 1486 5161 5529 5577
Size: 716 770 3675 368 48
Fragments arranged by size:
3675 770 716 368 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 991 1585 2011 2425 2572 2635 3361
Size: 926 65 594 426 414 147 63 726
Cuts at: 3361 3403 3821 3829 4377 4588 4959 5577
Size: 42 418 8 548 211 371 618
Fragments arranged by size:
926 726 618 594 548 426 418 414
371 211 147 65 63 42 8
Bpu10I CC'TnA_GC
Cuts at: 0 846 2908 3263 3481 3653 3822 5211 5577
Size: 846 2062 355 218 172 169 1389 366
Fragments arranged by size:
2062 1389 846 366 355 218 172 169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1456 2857 5174 5346 5577
Size: 1333 123 1401 2317 172 231
Fragments arranged by size:
2317 1401 1333 231 172 123
BsaI GGTCTCn'nnnn_
Cuts at: 0 3420 3530 4834 5577
Size: 3420 110 1304 743
Fragments arranged by size:
3420 1304 743 110
BsaBI GATnn'nnATC
Cuts at: 0 403 1492 5577
Size: 403 1089 4085
Fragments arranged by size:
4085 1089 403
BsaHI Gr'CG_yC
Cuts at: 0 4671 5577
Size: 4671 906
BsaWI w'CCGG_w
Cuts at: 0 494 3632 4253 5577
Size: 494 3138 621 1324
Fragments arranged by size:
3138 1324 621 494
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1066 1096 2246 2276 2608 2638 4957 4987
Size: 1066 30 1150 30 332 30 2319 30
Cuts at: 4987 5577
Size: 590
Fragments arranged by size:
2319 1150 1066 590 332 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 813 879 1087 2167 2569 2677 2854 3227
Size: 813 66 208 1080 402 108 177 373
Cuts at: 3227 4567 4852 5577
Size: 1340 285 725
Fragments arranged by size:
1340 1080 813 725 402 373 285 208
177 108 66
BseYI C'CCAG_C
Cuts at: 0 1594 1802 4081 5286 5368 5577
Size: 1594 208 2279 1205 82 209
Fragments arranged by size:
2279 1594 1205 209 208 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1597 4498 5078 5577
Size: 586 1011 2901 580 499
Fragments arranged by size:
2901 1011 586 580 499
BsiEI CG_ry'CG
Cuts at: 0 1257 5577
Size: 1257 4320
BsiHKAI G_wGCw'C
Cuts at: 0 82 548 1081 1220 1469 1613 2308 3401
Size: 82 466 533 139 249 144 695 1093
Cuts at: 3401 3654 3905 4238 4713 5577
Size: 253 251 333 475 864
Fragments arranged by size:
1093 864 695 533 475 466 333 253
251 249 144 139 82
BsmI GAATG_Cn'
Cuts at: 0 1689 2410 2541 5577
Size: 1689 721 131 3036
Fragments arranged by size:
3036 1689 721 131
Bsp1286I G_dGCh'C
Cuts at: 0 82 182 313 370 450 548 812 1081
Size: 82 100 131 57 80 98 264 269
Cuts at: 1081 1220 1469 1613 2308 2797 3120 3286 3401
Size: 139 249 144 695 489 323 166 115
Cuts at: 3401 3654 3737 3888 3905 4082 4238 4617 4630
Size: 253 83 151 17 177 156 379 13
Cuts at: 4630 4713 5320 5383 5525 5577
Size: 83 607 63 142 52
Fragments arranged by size:
695 607 489 379 323 269 264 253
249 177 166 156 151 144 142 139
131 115 100 98 83 83 82 80
63 57 52 17 13
BspEI T'CCGG_A
Cuts at: 0 494 3632 4253 5577
Size: 494 3138 621 1324
Fragments arranged by size:
3138 1324 621 494
BspHI T'CATG_A
Cuts at: 0 3401 5577
Size: 3401 2176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2990 5442 5577
Size: 2990 2452 135
Fragments arranged by size:
2990 2452 135
BsrBI CCG'CTC
Cuts at: 0 3591 5577
Size: 3591 1986
BsrDI GCAATG_nn'
Cuts at: 0 3343 4861 5577
Size: 3343 1518 716
Fragments arranged by size:
3343 1518 716
BsrFI r'CCGG_y
Cuts at: 0 4379 5577
Size: 4379 1198
BsrGI T'GTAC_A
Cuts at: 0 1490 4719 5577
Size: 1490 3229 858
Fragments arranged by size:
3229 1490 858
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3877 4512 5577
Size: 3877 635 1065
Fragments arranged by size:
3877 1065 635
BstEII G'GTnAC_C
Cuts at: 0 584 5577
Size: 584 4993
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2190 2550 3055 3315 4800 5181 5577
Size: 2190 360 505 260 1485 381 396
Fragments arranged by size:
2190 1485 505 396 381 360 260
BstYI r'GATC_y
Cuts at: 0 108 294 372 497 694 2064 3162 3176
Size: 108 186 78 125 197 1370 1098 14
Cuts at: 3176 4256 5550 5577
Size: 1080 1294 27
Fragments arranged by size:
1370 1294 1098 1080 197 186 125 108
78 27 14
Bsu36I CC'TnA_GG
Cuts at: 0 2660 2729 5577
Size: 2660 69 2848
Fragments arranged by size:
2848 2660 69
BtgI C'CryG_G
Cuts at: 0 2068 3975 5180 5577
Size: 2068 1907 1205 397
Fragments arranged by size:
2068 1907 1205 397
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1904 2474 2927 4676 5577
Size: 1904 570 453 1749 901
Fragments arranged by size:
1904 1749 901 570 453
BtsI GCAGTG_nn'
Cuts at: 0 857 981 1770 1989 2970 3602 3802 4617
Size: 857 124 789 219 981 632 200 815
Cuts at: 4617 4897 5446 5577
Size: 280 549 131
Fragments arranged by size:
981 857 815 789 632 549 280 219
200 131 124
DraI TTT'AAA
Cuts at: 0 149 597 5577
Size: 149 448 4980
Fragments arranged by size:
4980 448 149
DraIII CAC_nnn'GTG
Cuts at: 0 3256 5577
Size: 3256 2321
DrdI GACnn_nn'nnGTC
Cuts at: 0 3961 5577
Size: 3961 1616
EaeI y'GGCC_r
Cuts at: 0 3617 4578 5253 5531 5577
Size: 3617 961 675 278 46
Fragments arranged by size:
3617 961 675 278 46
EarI CTCTTCn'nnn_
Cuts at: 0 580 685 1225 2782 3115 3188 3428 3635
Size: 580 105 540 1557 333 73 240 207
Cuts at: 3635 3802 5152 5577
Size: 167 1350 425
Fragments arranged by size:
1557 1350 580 540 425 333 240 207
167 105 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2335 2883 4298 4564 5577
Size: 2335 548 1415 266 1013
Fragments arranged by size:
2335 1415 1013 548 266
EcoICRI GAG'CTC
Cuts at: 0 1218 1611 3399 3903 4236 5577
Size: 1218 393 1788 504 333 1341
Fragments arranged by size:
1788 1341 1218 504 393 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 394 926 991 1082 1585 2011 2295
Size: 242 152 532 65 91 503 426 284
Cuts at: 2295 2425 2572 2635 2856 3361 3403 3769 3821
Size: 130 147 63 221 505 42 366 52
Cuts at: 3821 3829 4377 4588 4959 5032 5577
Size: 8 548 211 371 73 545
Fragments arranged by size:
548 545 532 505 503 426 371 366
284 242 221 211 152 147 130 91
73 65 63 52 42 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1357 1654 3070 5577
Size: 1357 297 1416 2507
Fragments arranged by size:
2507 1416 1357 297
EcoO109I rG'GnC_Cy
Cuts at: 0 1016 2793 2794 3282 3884 4078 4093 5169
Size: 1016 1777 1 488 602 194 15 1076
Cuts at: 5169 5291 5577
Size: 122 286
Fragments arranged by size:
1777 1076 1016 602 488 286 194 122
15 1
EcoRI G'AATT_C
Cuts at: 0 883 5577
Size: 883 4694
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1704 5577
Size: 1704 3873
FspI TGC'GCA
Cuts at: 0 3673 5577
Size: 3673 1904
HaeII r_GCGC'y
Cuts at: 0 4674 5577
Size: 4674 903
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 239 271 1066 1093 1098 1125 1359 1391
Size: 239 32 795 27 5 27 234 32
Cuts at: 1391 3187 3219 3629 3636 3661 3668 3892 3921
Size: 1796 32 410 7 25 7 224 29
Cuts at: 3921 3924 3953 4144 4176 4972 5004 5263 5295
Size: 3 29 191 32 796 32 259 32
Cuts at: 5295 5577
Size: 282
Fragments arranged by size:
1796 796 795 410 282 259 239 234
224 191 32 32 32 32 32 32
29 29 27 27 25 7 7 5
3
HincII GTy'rAC
Cuts at: 0 267 1705 2004 3221 4026 5574 5577
Size: 267 1438 299 1217 805 1548 3
Fragments arranged by size:
1548 1438 1217 805 299 267 3
HindIII A'AGCT_T
Cuts at: 0 1501 5577
Size: 1501 4076
KasI G'GCGC_C
Cuts at: 0 4670 5577
Size: 4670 907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1670 2270 2404 3473 3977 5577
Size: 1670 600 134 1069 504 1600
Fragments arranged by size:
1670 1600 1069 600 504 134
MscI TGG'CCA
Cuts at: 0 3619 4580 5255 5533 5577
Size: 3619 961 675 278 44
Fragments arranged by size:
3619 961 675 278 44
MslI CAynn'nnrTG
Cuts at: 0 675 805 1519 2188 2717 3053 3554 3776
Size: 675 130 714 669 529 336 501 222
Cuts at: 3776 3920 4322 5179 5577
Size: 144 402 857 398
Fragments arranged by size:
857 714 675 669 529 501 402 398
336 222 144 130
MspA1I CmG'CkG
Cuts at: 0 570 3189 3381 3841 5041 5230 5577
Size: 570 2619 192 460 1200 189 347
Fragments arranged by size:
2619 1200 570 460 347 192 189
NaeI GCC'GGC
Cuts at: 0 4381 5577
Size: 4381 1196
NarI GG'CG_CC
Cuts at: 0 4671 5577
Size: 4671 906
NcoI C'CATG_G
Cuts at: 0 3975 5180 5577
Size: 3975 1205 397
Fragments arranged by size:
3975 1205 397
NdeI CA'TA_TG
Cuts at: 0 716 3441 5577
Size: 716 2725 2136
Fragments arranged by size:
2725 2136 716
NgoMIV G'CCGG_C
Cuts at: 0 4379 5577
Size: 4379 1198
NheI G'CTAG_C
Cuts at: 0 1864 5577
Size: 1864 3713
NspI r_CATG'y
Cuts at: 0 795 1461 2625 4405 4965 5022 5577
Size: 795 666 1164 1780 560 57 555
Fragments arranged by size:
1780 1164 795 666 560 555 57
PciI A'CATG_T
Cuts at: 0 2621 5577
Size: 2621 2956
PflMI CCAn_nnn'nTGG
Cuts at: 0 1122 1966 2789 5577
Size: 1122 844 823 2788
Fragments arranged by size:
2788 1122 844 823
PfoI T'CCnGG_A
Cuts at: 0 1007 2612 5165 5577
Size: 1007 1605 2553 412
Fragments arranged by size:
2553 1605 1007 412
PpuMI rG'GwC_Cy
Cuts at: 0 1016 5169 5577
Size: 1016 4153 408
Fragments arranged by size:
4153 1016 408
PshAI GACnn'nnGTC
Cuts at: 0 724 5577
Size: 724 4853
PspOMI G'GGCC_C
Cuts at: 0 2793 3282 3884 4078 4613 5577
Size: 2793 489 602 194 535 964
Fragments arranged by size:
2793 964 602 535 489 194
PstI C_TGCA'G
Cuts at: 0 3107 3851 4640 4706 4821 4886 5432 5453
Size: 3107 744 789 66 115 65 546 21
Cuts at: 5453 5577
Size: 124
Fragments arranged by size:
3107 789 744 546 124 115 66 65
21
PvuII CAG'CTG
Cuts at: 0 570 3381 3841 5577
Size: 570 2811 460 1736
Fragments arranged by size:
2811 1736 570 460
SacI G_AGCT'C
Cuts at: 0 1220 1613 3401 3905 4238 5577
Size: 1220 393 1788 504 333 1339
Fragments arranged by size:
1788 1339 1220 504 393 333
SanDI GG'GwC_CC
Cuts at: 0 1016 5577
Size: 1016 4561
SapI GCTCTTCn'nnn_
Cuts at: 0 580 1225 3115 5577
Size: 580 645 1890 2462
Fragments arranged by size:
2462 1890 645 580
SexAI A'CCwGG_T
Cuts at: 0 732 3719 5577
Size: 732 2987 1858
Fragments arranged by size:
2987 1858 732
SfcI C'TryA_G
Cuts at: 0 866 959 1325 1901 3103 3184 3391 3847
Size: 866 93 366 576 1202 81 207 456
Cuts at: 3847 4536 4636 4702 4817 4882 5428 5449 5577
Size: 689 100 66 115 65 546 21 128
Fragments arranged by size:
1202 866 689 576 546 456 366 207
128 115 100 93 81 66 65 21
SfoI GGC'GCC
Cuts at: 0 4672 5577
Size: 4672 905
SmaI CCC'GGG
Cuts at: 0 1141 5577
Size: 1141 4436
SmlI C'TyrA_G
Cuts at: 0 1312 1471 2836 4168 5189 5361 5577
Size: 1312 159 1365 1332 1021 172 216
Fragments arranged by size:
1365 1332 1312 1021 216 172 159
SphI G_CATG'C
Cuts at: 0 795 1461 5577
Size: 795 666 4116
Fragments arranged by size:
4116 795 666
SspI AAT'ATT
Cuts at: 0 15 5577
Size: 15 5562
StuI AGG'CCT
Cuts at: 0 1928 2174 5577
Size: 1928 246 3403
Fragments arranged by size:
3403 1928 246
StyI C'CwwG_G
Cuts at: 0 905 3131 3975 5180 5405 5435 5577
Size: 905 2226 844 1205 225 30 142
Fragments arranged by size:
2226 1205 905 844 225 142 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 118 316 364 433 1816 2556 4816
Size: 97 21 198 48 69 1383 740 2260
Cuts at: 4816 5577
Size: 761
Fragments arranged by size:
2260 1383 761 740 198 97 69 48
21
TatI w'GTAC_w
Cuts at: 0 1490 4719 4958 5577
Size: 1490 3229 239 619
Fragments arranged by size:
3229 1490 619 239
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1354 1386 2246 2278 2375 2407 4957 4989
Size: 1354 32 860 32 97 32 2550 32
Cuts at: 4989 5209 5241 5339 5371 5577
Size: 220 32 98 32 206
Fragments arranged by size:
2550 1354 860 220 206 98 97 32
32 32 32 32 32
XbaI T'CTAG_A
Cuts at: 0 924 5577
Size: 924 4653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 615 3727 4303 5577
Size: 615 3112 576 1274
Fragments arranged by size:
3112 1274 615 576
XmaI C'CCGG_G
Cuts at: 0 1139 5577
Size: 1139 4438
XmnI GAAnn'nnTTC
Cuts at: 0 212 1687 4342 5577
Size: 212 1475 2655 1235
Fragments arranged by size:
2655 1475 1235 212
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PciI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SacI SanDI SapI SexAI SfcI SfoI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AloI AscI AseI
AsiSI AvrII BaeI BciVI BfrBI BsaAI BsiWI BsmBI
BssHII BssSI BstBI BstZ17I ClaI CspCI EagI EcoRV
FseI FspAI HpaI KpnI MfeI MluI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I XhoI
ZraI