(Circular) (Six-base) MAPSORT of: pFN21ASDA0238.seq Check: 1416 from: 1 to: 6679 pFN21ASDA0238 6679 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4489 278 Size: 53 83 186 936 2953 2468 Fragments arranged by size: 2953 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4022 1937 Size: 2085 4594 Acc65I G'GTAC_C Cuts at: 2319 4000 2319 Size: 1681 4998 AclI AA'CG_TT Cuts at: 1563 2492 4806 5179 1563 Size: 929 2314 373 3063 Fragments arranged by size: 3063 2314 929 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2347 2759 3853 4742 6126 6394 1632 Size: 715 412 1094 889 1384 268 1917 Fragments arranged by size: 1917 1384 1094 889 715 412 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6482 AflIII A'CryG_T Cuts at: 1203 5578 6555 1203 Size: 4375 977 1327 Fragments arranged by size: 4375 1327 977 AgeI A'CCGG_T Cuts at: 5510 5510 Size: 6679 AhdI GACnn_n'nnGTC Cuts at: 5408 5408 Size: 6679 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6679 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2858 1184 Size: 1674 5005 AlwNI CAG_nnn'CTG Cuts at: 4422 5994 4422 Size: 1572 5107 ApaLI G'TGCA_C Cuts at: 3225 4736 5892 3225 Size: 1511 1156 4012 Fragments arranged by size: 4012 1511 1156 ApoI r'AATT_y Cuts at: 1493 2894 3276 3556 3988 4214 4366 6475 1493 Size: 1401 382 280 432 226 152 2109 1697 Fragments arranged by size: 2109 1697 1401 432 382 280 226 152 AseI AT'TA_AT Cuts at: 160 1032 5233 160 Size: 872 4201 1606 Fragments arranged by size: 4201 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6679 AvaI C'yCGr_G Cuts at: 1870 1943 2550 4004 1870 Size: 73 607 1454 4545 Fragments arranged by size: 4545 1454 607 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2311 2344 2311 Size: 33 6646 BamHI G'GATC_C Cuts at: 2655 3472 4009 4376 2655 Size: 817 537 367 4958 Fragments arranged by size: 4958 817 537 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2147 2319 4000 5455 618 Size: 325 217 627 360 172 1681 1455 1842 Fragments arranged by size: 1842 1681 1455 627 360 325 217 172 BanII G_rGCy'C Cuts at: 1391 2190 2234 2734 3490 3606 3998 1391 Size: 799 44 500 756 116 392 4072 Fragments arranged by size: 4072 799 756 500 392 116 44 BbeI G_GCGC'C Cuts at: 2151 2151 Size: 6679 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2100 3019 3960 928 Size: 971 201 919 941 3647 Fragments arranged by size: 3647 971 941 919 201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2362 2396 4870 4904 1903 Size: 34 425 34 2474 34 3678 Fragments arranged by size: 3678 2474 425 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2658 2998 3965 4571 5781 2658 Size: 340 967 606 1210 3556 Fragments arranged by size: 3556 1210 967 606 340 BclI T'GATC_A Cuts at: 1548 3513 1548 Size: 1965 4714 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2718 5290 136 Size: 107 122 71 631 605 1046 2572 1525 Fragments arranged by size: 2572 1525 1046 631 605 122 107 71 BglII A'GATC_T Cuts at: 6674 6674 Size: 6679 BlpI GC'TnA_GC Cuts at: 4096 4096 Size: 6679 Bme1580I G_kGCm'C Cuts at: 1790 2385 3229 3244 4740 5896 1790 Size: 595 844 15 1496 1156 2573 Fragments arranged by size: 2573 1496 1156 844 595 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6679 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2839 3333 4410 Size: 416 53 83 399 24 1422 494 1077 Cuts at: 4410 5368 442 Size: 958 1753 Fragments arranged by size: 1753 1422 1077 958 494 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6679 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3431 3431 Size: 6679 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2140 3732 5339 1239 Size: 901 1592 1607 2579 Fragments arranged by size: 2579 1607 1592 901 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4804 5669 5967 4804 Size: 865 298 5516 Fragments arranged by size: 5516 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2237 5342 882 Size: 617 738 3105 2219 Fragments arranged by size: 3105 2219 738 617 BsaAI yAC'GTr Cuts at: 493 1204 3976 4470 493 Size: 711 2772 494 2702 Fragments arranged by size: 2772 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6673 6673 Size: 6679 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 4486 4868 5503 Size: 53 83 186 936 615 2338 382 635 Cuts at: 5503 275 Size: 1451 Fragments arranged by size: 2338 1451 936 635 615 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2128 3469 3765 5112 5510 5784 5931 6342 2128 Size: 1341 296 1347 398 274 147 411 2465 Fragments arranged by size: 2465 1347 1341 411 398 296 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6454 Fragments arranged by size: 6454 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2705 1186 Size: 1519 5160 BseYI C'CCAG_C Cuts at: 2110 2274 3481 5882 6378 2110 Size: 164 1207 2401 496 2411 Fragments arranged by size: 2411 2401 1207 496 164 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3481 1163 Size: 573 1745 4361 Fragments arranged by size: 4361 1745 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2376 3156 4129 4890 5918 1540 Size: 275 186 375 780 973 761 1028 2301 Fragments arranged by size: 2301 1028 973 780 761 375 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2234 3229 4740 4825 5896 2190 Size: 44 995 1511 85 1071 2973 Fragments arranged by size: 2973 1511 1071 995 85 44 BsmI GAATG_Cn' Cuts at: 4190 4283 4190 Size: 93 6586 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2234 2385 2734 3229 3244 3490 Size: 399 400 44 151 349 495 15 246 Cuts at: 3490 3606 3998 4740 4825 5896 1391 Size: 116 392 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 495 400 399 392 349 246 151 116 85 44 15 BspEI T'CCGG_A Cuts at: 3469 3765 6342 3469 Size: 296 2577 3806 Fragments arranged by size: 3806 2577 296 BspHI T'CATG_A Cuts at: 4568 6298 4568 Size: 1730 4949 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2493 3546 4034 844 Size: 1649 1053 488 3489 Fragments arranged by size: 3489 1649 1053 488 BsrBI CCG'CTC Cuts at: 1394 1823 4566 1394 Size: 429 2743 3507 Fragments arranged by size: 3507 2743 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2217 2835 5174 5348 65 Size: 1183 969 618 2339 174 1396 Fragments arranged by size: 2339 1396 1183 969 618 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3155 5323 5510 1700 Size: 110 1345 2168 187 2869 Fragments arranged by size: 2869 2168 1345 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6679 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6679 BssSI C'ACGA_G Cuts at: 4739 5751 4739 Size: 1012 5667 BstAPI GCAn_nnn'nTGC Cuts at: 794 3085 794 Size: 2291 4388 BstBI TT'CG_AA Cuts at: 2007 5483 2007 Size: 3476 3203 BstEII G'GTnAC_C Cuts at: 1184 2505 1184 Size: 1321 5358 BstXI CCAn_nnnn'nTGG Cuts at: 2059 2410 2444 3129 2059 Size: 351 34 685 5609 Fragments arranged by size: 5609 685 351 34 BstYI r'GATC_y Cuts at: 1972 2655 2664 3472 4009 4376 4761 4778 6230 Size: 683 9 808 537 367 385 17 1452 Cuts at: 6230 6316 6328 6674 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 808 683 537 385 367 346 86 17 12 9 Bsu36I CC'TnA_GG Cuts at: 3920 3920 Size: 6679 BtgI C'CryG_G Cuts at: 513 1065 2769 3101 3180 3966 513 Size: 552 1704 332 79 786 3226 Fragments arranged by size: 3226 1704 786 552 332 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5940 Fragments arranged by size: 5940 683 56 BtsI GCAGTG_nn' Cuts at: 2385 4195 4989 5009 2385 Size: 1810 794 20 4055 Fragments arranged by size: 4055 1810 794 20 ClaI AT'CG_AT Cuts at: 1554 3660 1554 Size: 2106 4573 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3879 3914 529 Size: 35 3315 35 3294 Fragments arranged by size: 3315 3294 35 35 DraI TTT'AAA Cuts at: 3555 3983 3555 Size: 428 6251 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5686 809 Size: 507 4370 1802 Fragments arranged by size: 4370 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2976 3153 4126 Size: 54 1425 50 275 18 1146 177 973 Cuts at: 4126 5015 6644 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1146 973 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3153 4126 1537 Size: 275 1341 973 4090 Fragments arranged by size: 4090 1341 973 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2130 3920 4609 1353 Size: 777 1790 689 3423 Fragments arranged by size: 3423 1790 777 689 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5266 5640 5786 387 Size: 1064 138 3677 374 146 1280 Fragments arranged by size: 3677 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2188 2232 2188 Size: 44 6635 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2347 2759 3732 3853 4742 5339 Size: 393 508 207 412 973 121 889 597 Cuts at: 5339 6126 6394 1239 Size: 787 268 1524 Fragments arranged by size: 1524 973 889 787 597 508 412 393 268 207 121 EcoNI CCTnn'n_nnAGG Cuts at: 2516 2516 Size: 6679 EcoRI G'AATT_C Cuts at: 3988 4366 3988 Size: 378 6301 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3070 3104 3070 Size: 34 6645 FspI TGC'GCA Cuts at: 5185 5185 Size: 6679 HaeII r_GCGC'y Cuts at: 1678 2151 3453 5826 1678 Size: 473 1302 2373 2531 Fragments arranged by size: 2531 2373 1302 473 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3576 3608 3941 3973 5320 Size: 32 135 32 2004 32 333 32 1347 Cuts at: 5320 5352 5394 5426 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2004 1347 333 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4023 4269 669 Size: 988 281 2085 246 3079 Fragments arranged by size: 3079 2085 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6679 HpaI GTT'AAC Cuts at: 4269 4269 Size: 6679 KasI G'GCGC_C Cuts at: 2147 2147 Size: 6679 KpnI G_GTAC'C Cuts at: 2323 4004 2323 Size: 1681 4998 MfeI C'AATT_G Cuts at: 4278 4278 Size: 6679 MluI A'CGCG_T Cuts at: 6555 6555 Size: 6679 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5793 5977 1305 Size: 4488 184 2007 Fragments arranged by size: 4488 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2978 10 Size: 54 1425 343 1146 3711 Fragments arranged by size: 3711 1425 1146 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 2681 2840 3127 3167 4637 4996 Size: 648 1242 273 159 287 40 1470 359 Cuts at: 4996 5155 518 Size: 159 2042 Fragments arranged by size: 2042 1470 1242 648 359 287 273 159 159 40 MspA1I CmG'CkG Cuts at: 1606 2160 2367 2807 2950 3521 4095 4772 5920 Size: 554 207 440 143 571 574 677 1148 Cuts at: 5920 6165 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 574 571 554 440 245 207 143 NarI GG'CG_CC Cuts at: 2148 2148 Size: 6679 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6127 NdeI CA'TA_TG Cuts at: 387 387 Size: 6679 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6679 NotI GC'GGCC_GC Cuts at: 4126 4126 Size: 6679 NspI r_CATG'y Cuts at: 1132 1229 2361 3172 4037 5582 1132 Size: 97 1132 811 865 1545 2229 Fragments arranged by size: 2229 1545 1132 865 811 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6679 PciI A'CATG_T Cuts at: 5578 5578 Size: 6679 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6679 PfoI T'CCnGG_A Cuts at: 3563 3723 3563 Size: 160 6519 PmeI GTTT'AAAC Cuts at: 3983 3983 Size: 6679 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4748 4780 6261 6293 4748 Size: 32 1481 32 5134 Fragments arranged by size: 5134 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3023 1613 Size: 1410 5269 PsiI TTA'TAA Cuts at: 2913 3851 4249 2913 Size: 938 398 5343 Fragments arranged by size: 5343 938 398 PspXI vC'TCGA_Gb Cuts at: 2550 2550 Size: 6679 PstI C_TGCA'G Cuts at: 830 3543 4031 830 Size: 2713 488 3478 Fragments arranged by size: 3478 2713 488 PvuI CG_AT'CG Cuts at: 2001 2376 2001 Size: 375 6304 PvuII CAG'CTG Cuts at: 2950 3521 2950 Size: 571 6108 SacI G_AGCT'C Cuts at: 2190 2234 2190 Size: 44 6635 SalI G'TCGA_C Cuts at: 1936 4021 1936 Size: 2085 4594 SapI GCTCTTCn'nnn_ Cuts at: 3920 3920 Size: 6679 SbfI CC_TGCA'GG Cuts at: 4031 4031 Size: 6679 ScaI AGT'ACT Cuts at: 4927 6639 4927 Size: 1712 4967 SfcI C'TryA_G Cuts at: 826 1045 2342 2483 3539 4027 5162 5843 6034 Size: 219 1297 141 1056 488 1135 681 191 Cuts at: 6034 826 Size: 1471 Fragments arranged by size: 1471 1297 1135 1056 681 488 219 191 141 SfiI GGCCn_nnn'nGGCC Cuts at: 2718 2718 Size: 6679 SfoI GGC'GCC Cuts at: 2149 2149 Size: 6679 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6679 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6679 SmaI CCC'GGG Cuts at: 1872 4006 1872 Size: 2134 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2550 4783 5684 5946 820 Size: 197 926 607 2233 901 262 1553 Fragments arranged by size: 2233 1553 926 901 607 262 197 SnaBI TAC'GTA Cuts at: 493 3976 4470 493 Size: 3483 494 2702 Fragments arranged by size: 3483 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6679 SphI G_CATG'C Cuts at: 1132 3172 4037 1132 Size: 2040 865 3774 Fragments arranged by size: 3774 2040 865 SspI AAT'ATT Cuts at: 5 52 2853 4603 5 Size: 47 2801 1750 2081 Fragments arranged by size: 2801 2081 1750 47 StuI AGG'CCT Cuts at: 1517 3291 3918 1517 Size: 1774 627 4278 Fragments arranged by size: 4278 1774 627 StyI C'CwwG_G Cuts at: 513 1065 4118 513 Size: 552 3053 3074 Fragments arranged by size: 3074 3053 552 SwaI ATTT'AAAT Cuts at: 3555 3555 Size: 6679 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2124 2738 2782 4706 4723 4876 5061 Size: 1255 544 614 44 1924 17 153 185 Cuts at: 5061 5506 325 Size: 445 1498 Fragments arranged by size: 1924 1498 1255 614 544 445 185 153 44 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2742 4925 6637 Size: 274 80 33 51 1443 765 2183 1712 Cuts at: 6637 96 Size: 138 Fragments arranged by size: 2183 1712 1443 765 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3267 3299 3766 3798 6639 6671 3267 Size: 32 467 32 2841 32 3275 Fragments arranged by size: 3275 2841 467 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6679 XbaI T'CTAG_A Cuts at: 4015 4015 Size: 6679 XhoI C'TCGA_G Cuts at: 1943 2550 1943 Size: 607 6072 XmaI C'CCGG_G Cuts at: 1870 4004 1870 Size: 2134 4545 XmnI GAAnn'nnTTC Cuts at: 2307 4808 2307 Size: 2501 4178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4487 276 Size: 53 83 186 936 2953 2468 Fragments arranged by size: 2953 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SrfI XcmI