(Circular) (Six-base) MAPSORT of: pFN21ASDA0237.seq Check: 6951 from: 1 to: 5653 pFN21ASDA0237 5653 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2725 2725 Size: 5653 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 1937 Size: 1059 4594 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2259 3716 5100 5368 1632 Size: 627 1457 1384 268 1917 Fragments arranged by size: 1917 1457 1384 627 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2747 1184 Size: 1563 4090 AlwNI CAG_nnn'CTG Cuts at: 2045 2476 2698 3396 4968 2045 Size: 431 222 698 1572 2730 Fragments arranged by size: 2730 1572 698 431 222 ApaLI G'TGCA_C Cuts at: 2501 3710 4866 2501 Size: 1209 1156 3288 Fragments arranged by size: 3288 1209 1156 ApoI r'AATT_y Cuts at: 1493 2962 3188 3340 5449 1493 Size: 1469 226 152 2109 1697 Fragments arranged by size: 2109 1697 1469 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2556 2978 1870 Size: 73 613 422 4545 Fragments arranged by size: 4545 613 422 73 BamHI G'GATC_C Cuts at: 2108 2885 2983 3350 2108 Size: 777 98 367 4411 Fragments arranged by size: 4411 777 367 98 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2171 2898 2974 4429 618 Size: 325 217 627 384 727 76 1455 1842 Fragments arranged by size: 1842 1455 727 627 384 325 217 76 BanII G_rGCy'C Cuts at: 1391 2084 2972 1391 Size: 693 888 4072 Fragments arranged by size: 4072 888 693 BbeI G_GCGC'C Cuts at: 2902 2902 Size: 5653 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2657 2675 2904 928 Size: 971 758 18 229 3677 Fragments arranged by size: 3677 971 758 229 18 BbvCI CC'TCA_GC Cuts at: 2819 2819 Size: 5653 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2639 4264 136 Size: 107 122 71 631 605 967 1625 1525 Fragments arranged by size: 1625 1525 967 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2505 3714 4870 1790 Size: 715 1209 1156 2573 Fragments arranged by size: 2573 1209 1156 715 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2902 3384 4342 Size: 416 53 83 399 24 1485 482 958 Cuts at: 4342 442 Size: 1753 Fragments arranged by size: 1753 1485 958 482 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2047 2068 2207 2555 2642 2939 4313 1239 Size: 808 21 139 348 87 297 1374 2579 Fragments arranged by size: 2579 1374 808 348 297 139 87 21 Bpu10I CC'TnA_GC Cuts at: 2819 2819 Size: 5653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2950 3444 493 Size: 711 1746 494 2702 Fragments arranged by size: 2702 1746 711 494 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2899 3460 3842 4477 Size: 53 83 186 936 1366 561 382 635 Cuts at: 4477 275 Size: 1451 Fragments arranged by size: 1451 1366 936 635 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2520 4086 4484 4758 4905 5316 2520 Size: 1566 398 274 147 411 2857 Fragments arranged by size: 2857 1566 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5428 Fragments arranged by size: 5428 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2851 1186 Size: 1665 3988 BseYI C'CCAG_C Cuts at: 2112 2132 2443 2593 4856 5352 2112 Size: 20 311 150 2263 496 2413 Fragments arranged by size: 2413 2263 496 311 150 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5080 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2084 2219 2282 2357 2505 3714 3799 4870 2084 Size: 135 63 75 148 1209 85 1071 2867 Fragments arranged by size: 2867 1209 1071 148 135 85 75 63 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2219 2282 2357 2505 2972 3714 Size: 399 294 135 63 75 148 467 742 Cuts at: 3714 3799 4870 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 467 399 294 148 135 85 75 63 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2625 2725 3008 844 Size: 1781 100 283 3489 Fragments arranged by size: 3489 1781 283 100 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2505 4148 4322 65 Size: 1183 1257 1643 174 1396 Fragments arranged by size: 1643 1396 1257 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2834 4297 4484 1700 Size: 110 1024 1463 187 2869 Fragments arranged by size: 2869 1463 1024 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 2698 794 Size: 1904 3749 BstBI TT'CG_AA Cuts at: 2007 4457 2007 Size: 2450 3203 BstEII G'GTnAC_C Cuts at: 1184 2830 1184 Size: 1646 4007 BstXI CCAn_nnnn'nTGG Cuts at: 2071 2182 2071 Size: 111 5542 BstYI r'GATC_y Cuts at: 1972 2108 2885 2983 3350 3735 3752 5204 5290 Size: 136 777 98 367 385 17 1452 86 Cuts at: 5290 5302 5648 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 777 385 367 346 136 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2490 2826 513 Size: 552 1425 336 3340 Fragments arranged by size: 3340 1425 552 336 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2278 487 Size: 683 56 1052 3862 Fragments arranged by size: 3862 1052 683 56 BtsI GCAGTG_nn' Cuts at: 3169 3963 3983 3169 Size: 794 20 4839 Fragments arranged by size: 4839 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2527 2640 Size: 54 1425 50 275 18 354 343 113 Cuts at: 2640 2758 3100 3989 5618 8 Size: 118 342 889 1629 43 Fragments arranged by size: 1629 1425 889 354 343 342 275 118 113 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2855 3583 1353 Size: 1502 728 3423 Fragments arranged by size: 3423 1502 728 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2173 2261 4240 4614 4760 387 Size: 1064 138 584 88 1979 374 146 1280 Fragments arranged by size: 1979 1280 1064 584 374 146 138 88 EcoICRI GAG'CTC Cuts at: 2082 2082 Size: 5653 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2068 2207 2259 2555 2642 2939 Size: 393 415 21 139 52 296 87 297 Cuts at: 2939 3716 4313 5100 5368 1239 Size: 777 597 787 268 1524 Fragments arranged by size: 1524 787 777 597 415 393 297 296 268 139 87 52 21 EcoO109I rG'GnC_Cy Cuts at: 2042 2058 2560 2631 2714 2042 Size: 16 502 71 83 4981 Fragments arranged by size: 4981 502 83 71 16 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FspI TGC'GCA Cuts at: 2261 4159 2261 Size: 1898 3755 HaeII r_GCGC'y Cuts at: 1678 2826 2902 4800 1678 Size: 1148 76 1898 2531 Fragments arranged by size: 2531 1898 1148 76 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2539 2571 2662 2694 4294 Size: 32 135 32 967 32 91 32 1600 Cuts at: 4294 4326 4368 4400 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1600 967 135 91 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KasI G'GCGC_C Cuts at: 2898 2898 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2397 4767 4951 1305 Size: 1092 2370 184 2007 Fragments arranged by size: 2370 2007 1092 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 2529 2642 10 Size: 54 1425 343 354 343 113 3021 Fragments arranged by size: 3021 1425 354 343 343 113 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 2786 3611 3970 4129 518 Size: 648 1131 489 825 359 159 2042 Fragments arranged by size: 2042 1131 825 648 489 359 159 MspA1I CmG'CkG Cuts at: 1606 2533 2828 3069 3746 4894 5139 1606 Size: 927 295 241 677 1148 245 2120 Fragments arranged by size: 2120 1148 927 677 295 245 241 NarI GG'CG_CC Cuts at: 2899 2899 Size: 5653 NcoI C'CATG_G Cuts at: 513 1065 2490 513 Size: 552 1425 3676 Fragments arranged by size: 3676 1425 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 2719 1348 Size: 1371 4282 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PfoI T'CCnGG_A Cuts at: 2063 2433 2063 Size: 370 5283 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2042 2714 2042 Size: 672 4981 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PstI C_TGCA'G Cuts at: 830 2736 3005 830 Size: 1906 269 3478 Fragments arranged by size: 3478 1906 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2533 2533 Size: 5653 SacI G_AGCT'C Cuts at: 2084 2084 Size: 5653 SacII CC_GC'GG Cuts at: 2829 2829 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SanDI GG'GwC_CC Cuts at: 2714 2714 Size: 5653 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2732 3001 4136 4817 5008 826 Size: 219 1687 269 1135 681 191 1471 Fragments arranged by size: 1687 1471 1135 681 269 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2639 2639 Size: 5653 SfoI GGC'GCC Cuts at: 2900 2900 Size: 5653 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3757 4658 4920 820 Size: 197 926 1814 901 262 1553 Fragments arranged by size: 1814 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2950 3444 493 Size: 2457 494 2702 Fragments arranged by size: 2702 2457 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2490 3092 513 Size: 552 1425 602 3074 Fragments arranged by size: 3074 1425 602 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 2503 3680 3697 3850 4035 4480 Size: 1255 579 344 1177 17 153 185 445 Cuts at: 4480 325 Size: 1498 Fragments arranged by size: 1498 1255 1177 579 445 344 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3899 5611 96 Size: 274 80 33 51 1443 1922 1712 138 Fragments arranged by size: 1922 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2759 2791 5613 5645 2759 Size: 32 2822 32 2767 Fragments arranged by size: 2822 2767 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2863 2863 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SapI SexAI SrfI SwaI