Restriction map for pFN21ASDA0237
 (Circular) (Six-base) MAPSORT of: pFN21ASDA0237.seq  Check: 6951  from: 1  to: 5653



pFN21ASDA0237  5653 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2725    2725 
   Size:       5653
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3463     278 
   Size:         53      83     186     936    1927    2468

  Fragments arranged by size: 

                2468    1927     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2996    1937 
   Size:       1059    4594
 
Acc65I G'GTAC_C

Cuts at:   2974    2974 
   Size:       5653
 
AclI AA'CG_TT

Cuts at:   1563    3780    4153    1563 
   Size:       2217     373    3063

  Fragments arranged by size: 

                3063    2217     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2259    3716    5100    5368    1632 
   Size:        627    1457    1384     268    1917

  Fragments arranged by size: 

                1917    1457    1384     627     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5456
 
AflIII A'CryG_T

Cuts at:   1203    4552    5529    1203 
   Size:       3349     977    1327

  Fragments arranged by size: 

                3349    1327     977
 
AgeI A'CCGG_T

Cuts at:   4484    4484 
   Size:       5653
 
AhdI GACnn_n'nnGTC

Cuts at:   4382    4382 
   Size:       5653
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5653
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2747    1184 
   Size:       1563    4090
 
AlwNI CAG_nnn'CTG

Cuts at:   2045    2476    2698    3396    4968    2045 
   Size:        431     222     698    1572    2730

  Fragments arranged by size: 

                2730    1572     698     431     222
 
ApaLI G'TGCA_C

Cuts at:   2501    3710    4866    2501 
   Size:       1209    1156    3288

  Fragments arranged by size: 

                3288    1209    1156
 
ApoI r'AATT_y

Cuts at:   1493    2962    3188    3340    5449    1493 
   Size:       1469     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1469     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4207     160 
   Size:        872    3175    1606

  Fragments arranged by size: 

                3175    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5653
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2556    2978    1870 
   Size:         73     613     422    4545

  Fragments arranged by size: 

                4545     613     422      73
 
BamHI G'GATC_C

Cuts at:   2108    2885    2983    3350    2108 
   Size:        777      98     367    4411

  Fragments arranged by size: 

                4411     777     367      98
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2171    2898    2974    4429     618 
   Size:        325     217     627     384     727      76    1455    1842

  Fragments arranged by size: 

                1842    1455     727     627     384     325     217      76
            
 
BanII G_rGCy'C

Cuts at:   1391    2084    2972    1391 
   Size:        693     888    4072

  Fragments arranged by size: 

                4072     888     693
 
BbeI G_GCGC'C

Cuts at:   2902    2902 
   Size:       5653
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2657    2675    2904     928 
   Size:        971     758      18     229    3677

  Fragments arranged by size: 

                3677     971     758     229      18
 
BbvCI CC'TCA_GC

Cuts at:   2819    2819 
   Size:       5653
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3844    3878    1903 
   Size:         34    1907      34    3678

  Fragments arranged by size: 

                3678    1907      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3545    4755    3545 
   Size:       1210    4443
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5653
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2639    4264     136 
   Size:        107     122      71     631     605     967    1625    1525

  Fragments arranged by size: 

                1625    1525     967     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5648    5648 
   Size:       5653
 
BlpI GC'TnA_GC

Cuts at:   3070    3070 
   Size:       5653
 
Bme1580I G_kGCm'C

Cuts at:   1790    2505    3714    4870    1790 
   Size:        715    1209    1156    2573

  Fragments arranged by size: 

                2573    1209    1156     715
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5653
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2902    3384    4342 
   Size:        416      53      83     399      24    1485     482     958

Cuts at:   4342     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1485     958     482     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5653
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2047    2068    2207    2555    2642    2939    4313    1239 
   Size:        808      21     139     348      87     297    1374    2579

  Fragments arranged by size: 

                2579    1374     808     348     297     139      87      21
            
 
Bpu10I CC'TnA_GC

Cuts at:   2819    2819 
   Size:       5653
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3778    4643    4941    3778 
   Size:        865     298    4490

  Fragments arranged by size: 

                4490     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4316     882 
   Size:        617    2817    2219

  Fragments arranged by size: 

                2817    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2950    3444     493 
   Size:        711    1746     494    2702

  Fragments arranged by size: 

                2702    1746     711     494
 
BsaBI GATnn'nnATC

Cuts at:   5647    5647 
   Size:       5653
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2899    3460    3842    4477 
   Size:         53      83     186     936    1366     561     382     635

Cuts at:   4477     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1366     936     635     561     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2520    4086    4484    4758    4905    5316    2520 
   Size:       1566     398     274     147     411    2857

  Fragments arranged by size: 

                2857    1566     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5428

  Fragments arranged by size: 

                5428     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2851    1186 
   Size:       1665    3988
 
BseYI C'CCAG_C

Cuts at:   2112    2132    2443    2593    4856    5352    2112 
   Size:         20     311     150    2263     496    2413

  Fragments arranged by size: 

                2413    2263     496     311     150      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5080
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3103    3864    4892    1540 
   Size:        275     186    1102     761    1028    2301

  Fragments arranged by size: 

                2301    1102    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2084    2219    2282    2357    2505    3714    3799    4870    2084 
   Size:        135      63      75     148    1209      85    1071    2867

  Fragments arranged by size: 

                2867    1209    1071     148     135      85      75      63
            
 
BsmI GAATG_Cn'

Cuts at:   3164    3257    3164 
   Size:         93    5560
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2084    2219    2282    2357    2505    2972    3714 
   Size:        399     294     135      63      75     148     467     742

Cuts at:   3714    3799    4870    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     467     399     294     148     135
                  85      75      63
 
BspEI T'CCGG_A

Cuts at:   5316    5316 
   Size:       5653
 
BspHI T'CATG_A

Cuts at:   3542    5272    3542 
   Size:       1730    3923
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2625    2725    3008     844 
   Size:       1781     100     283    3489

  Fragments arranged by size: 

                3489    1781     283     100
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3540    1394 
   Size:        429    1717    3507

  Fragments arranged by size: 

                3507    1717     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2505    4148    4322      65 
   Size:       1183    1257    1643     174    1396

  Fragments arranged by size: 

                1643    1396    1257    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2834    4297    4484    1700 
   Size:        110    1024    1463     187    2869

  Fragments arranged by size: 

                2869    1463    1024     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5653
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5653
 
BssSI C'ACGA_G

Cuts at:   3713    4725    3713 
   Size:       1012    4641
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2698     794 
   Size:       1904    3749
 
BstBI TT'CG_AA

Cuts at:   2007    4457    2007 
   Size:       2450    3203
 
BstEII G'GTnAC_C

Cuts at:   1184    2830    1184 
   Size:       1646    4007
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2071    2182    2071 
   Size:        111    5542
 
BstYI r'GATC_y

Cuts at:   1972    2108    2885    2983    3350    3735    3752    5204    5290 
   Size:        136     777      98     367     385      17    1452      86

Cuts at:   5290    5302    5648    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     777     385     367     346     136      98
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2490    2826     513 
   Size:        552    1425     336    3340

  Fragments arranged by size: 

                3340    1425     552     336
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2278     487 
   Size:        683      56    1052    3862

  Fragments arranged by size: 

                3862    1052     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3169    3963    3983    3169 
   Size:        794      20    4839

  Fragments arranged by size: 

                4839     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5653
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5618
 
DraI TTT'AAA

Cuts at:   2957    2957 
   Size:       5653
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4660     809 
   Size:        507    3344    1802

  Fragments arranged by size: 

                3344    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2184    2527    2640 
   Size:         54    1425      50     275      18     354     343     113

Cuts at:   2640    2758    3100    3989    5618       8 
   Size:        118     342     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     354     343     342     275     118
                 113      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3100    1537 
   Size:        275    1288    4090

  Fragments arranged by size: 

                4090    1288     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2855    3583    1353 
   Size:       1502     728    3423

  Fragments arranged by size: 

                3423    1502     728
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2173    2261    4240    4614    4760     387 
   Size:       1064     138     584      88    1979     374     146    1280

  Fragments arranged by size: 

                1979    1280    1064     584     374     146     138      88
            
 
EcoICRI GAG'CTC

Cuts at:   2082    2082 
   Size:       5653
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2047    2068    2207    2259    2555    2642    2939 
   Size:        393     415      21     139      52     296      87     297

Cuts at:   2939    3716    4313    5100    5368    1239 
   Size:        777     597     787     268    1524

  Fragments arranged by size: 

                1524     787     777     597     415     393     297     296
                 268     139      87      52      21
 
EcoO109I rG'GnC_Cy

Cuts at:   2042    2058    2560    2631    2714    2042 
   Size:         16     502      71      83    4981

  Fragments arranged by size: 

                4981     502      83      71      16
 
EcoRI G'AATT_C

Cuts at:   2962    3340    2962 
   Size:        378    5275
 
FspI TGC'GCA

Cuts at:   2261    4159    2261 
   Size:       1898    3755
 
HaeII r_GCGC'y

Cuts at:   1678    2826    2902    4800    1678 
   Size:       1148      76    1898    2531

  Fragments arranged by size: 

                2531    1898    1148      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2539    2571    2662    2694    4294 
   Size:         32     135      32     967      32      91      32    1600

Cuts at:   4294    4326    4368    4400    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1600     967     135      91      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2997    3243     669 
   Size:        988     281    1059     246    3079

  Fragments arranged by size: 

                3079    1059     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5653
 
HpaI GTT'AAC

Cuts at:   3243    3243 
   Size:       5653
 
KasI G'GCGC_C

Cuts at:   2898    2898 
   Size:       5653
 
KpnI G_GTAC'C

Cuts at:   2978    2978 
   Size:       5653
 
MfeI C'AATT_G

Cuts at:   3252    3252 
   Size:       5653
 
MluI A'CGCG_T

Cuts at:   5529    5529 
   Size:       5653
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2397    4767    4951    1305 
   Size:       1092    2370     184    2007

  Fragments arranged by size: 

                2370    2007    1092     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2186    2529    2642      10 
   Size:         54    1425     343     354     343     113    3021

  Fragments arranged by size: 

                3021    1425     354     343     343     113      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2297    2786    3611    3970    4129     518 
   Size:        648    1131     489     825     359     159    2042

  Fragments arranged by size: 

                2042    1131     825     648     489     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2533    2828    3069    3746    4894    5139    1606 
   Size:        927     295     241     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     927     677     295     245     241
 
NarI GG'CG_CC

Cuts at:   2899    2899 
   Size:       5653
 
NcoI C'CATG_G

Cuts at:    513    1065    2490     513 
   Size:        552    1425    3676

  Fragments arranged by size: 

                3676    1425     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5653
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5653
 
NotI GC'GGCC_GC

Cuts at:   3100    3100 
   Size:       5653
 
NspI r_CATG'y

Cuts at:   1132    1229    3011    4556    1132 
   Size:         97    1782    1545    2229

  Fragments arranged by size: 

                2229    1782    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2719    1348 
   Size:       1371    4282
 
PciI A'CATG_T

Cuts at:   4552    4552 
   Size:       5653
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5653
 
PfoI T'CCnGG_A

Cuts at:   2063    2433    2063 
   Size:        370    5283
 
PmeI GTTT'AAAC

Cuts at:   2957    2957 
   Size:       5653
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3722    3754    5235    5267    3722 
   Size:         32    1481      32    4108

  Fragments arranged by size: 

                4108    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2042    2714    2042 
   Size:        672    4981
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5653
 
PsiI TTA'TAA

Cuts at:   3223    3223 
   Size:       5653
 
PstI C_TGCA'G

Cuts at:    830    2736    3005     830 
   Size:       1906     269    3478

  Fragments arranged by size: 

                3478    1906     269
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5653
 
PvuII CAG'CTG

Cuts at:   2533    2533 
   Size:       5653
 
SacI G_AGCT'C

Cuts at:   2084    2084 
   Size:       5653
 
SacII CC_GC'GG

Cuts at:   2829    2829 
   Size:       5653
 
SalI G'TCGA_C

Cuts at:   1936    2995    1936 
   Size:       1059    4594
 
SanDI GG'GwC_CC

Cuts at:   2714    2714 
   Size:       5653
 
SbfI CC_TGCA'GG

Cuts at:   3005    3005 
   Size:       5653
 
ScaI AGT'ACT

Cuts at:   3901    5613    3901 
   Size:       1712    3941
 
SfcI C'TryA_G

Cuts at:    826    1045    2732    3001    4136    4817    5008     826 
   Size:        219    1687     269    1135     681     191    1471

  Fragments arranged by size: 

                1687    1471    1135     681     269     219     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2639    2639 
   Size:       5653
 
SfoI GGC'GCC

Cuts at:   2900    2900 
   Size:       5653
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5653
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5653
 
SmaI CCC'GGG

Cuts at:   1872    2980    1872 
   Size:       1108    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3757    4658    4920     820 
   Size:        197     926    1814     901     262    1553

  Fragments arranged by size: 

                1814    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2950    3444     493 
   Size:       2457     494    2702

  Fragments arranged by size: 

                2702    2457     494
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5653
 
SphI G_CATG'C

Cuts at:   1132    3011    1132 
   Size:       1879    3774
 
SspI AAT'ATT

Cuts at:      5      52    3577       5 
   Size:         47    3525    2081

  Fragments arranged by size: 

                3525    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5653
 
StyI C'CwwG_G

Cuts at:    513    1065    2490    3092     513 
   Size:        552    1425     602    3074

  Fragments arranged by size: 

                3074    1425     602     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2159    2503    3680    3697    3850    4035    4480 
   Size:       1255     579     344    1177      17     153     185     445

Cuts at:   4480     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1177     579     445     344     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3899    5611      96 
   Size:        274      80      33      51    1443    1922    1712     138

  Fragments arranged by size: 

                1922    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2759    2791    5613    5645    2759 
   Size:         32    2822      32    2767

  Fragments arranged by size: 

                2822    2767      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5653
 
XbaI T'CTAG_A

Cuts at:   2989    2989 
   Size:       5653
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2863    2863 
   Size:       5653
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5653
 
XmaI C'CCGG_G

Cuts at:   1870    2978    1870 
   Size:       1108    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3782    3782 
   Size:       5653
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3461     276 
   Size:         53      83     186     936    1927    2468

  Fragments arranged by size: 

                2468    1927     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII     SacI
    SacII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BfrBI     BplI
    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII    EcoNI    EcoRV     FalI
     FseI    FspAI     NaeI   NgoMIV     NruI     NsiI     PacI     PmlI
   PspOMI    PspXI     PsrI    RsrII     SapI    SexAI     SrfI     SwaI
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