Restriction map for
pFN21ASDA0237
(Linear) (Six-base) MAPSORT of: KIAA0237.seq Check: 5513 from: 1 to: 924
KIAA0237.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 702 924
Size: 702 222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 924
Size: 236 688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 724 924
Size: 724 200
AlwNI CAG_nnn'CTG
Cuts at: 0 22 453 675 924
Size: 22 431 222 249
Fragments arranged by size:
431 249 222 22
ApaLI G'TGCA_C
Cuts at: 0 478 924
Size: 478 446
AvaI C'yCGr_G
Cuts at: 0 533 924
Size: 533 391
BamHI G'GATC_C
Cuts at: 0 85 862 924
Size: 85 777 62
Fragments arranged by size:
777 85 62
BanI G'GyrC_C
Cuts at: 0 148 875 924
Size: 148 727 49
Fragments arranged by size:
727 148 49
BanII G_rGCy'C
Cuts at: 0 61 924
Size: 61 863
BbeI G_GCGC'C
Cuts at: 0 879 924
Size: 879 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 634 652 881 924
Size: 634 18 229 43
Fragments arranged by size:
634 229 43 18
BbvCI CC'TCA_GC
Cuts at: 0 796 924
Size: 796 128
BglI GCCn_nnn'nGGC
Cuts at: 0 616 924
Size: 616 308
Bme1580I G_kGCm'C
Cuts at: 0 482 924
Size: 482 442
BmrI ACTGGGnnnn_n'
Cuts at: 0 879 924
Size: 879 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 45 184 532 619 916 924
Size: 24 21 139 348 87 297 8
Fragments arranged by size:
348 297 139 87 24 21 8
Bpu10I CC'TnA_GC
Cuts at: 0 796 924
Size: 796 128
BsaHI Gr'CG_yC
Cuts at: 0 876 924
Size: 876 48
BsaWI w'CCGG_w
Cuts at: 0 497 924
Size: 497 427
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 828 924
Size: 828 96
BseYI C'CCAG_C
Cuts at: 0 89 109 420 570 924
Size: 89 20 311 150 354
Fragments arranged by size:
354 311 150 89 20
BsiHKAI G_wGCw'C
Cuts at: 0 61 196 259 334 482 924
Size: 61 135 63 75 148 442
Fragments arranged by size:
442 148 135 75 63 61
Bsp1286I G_dGCh'C
Cuts at: 0 61 196 259 334 482 924
Size: 61 135 63 75 148 442
Fragments arranged by size:
442 148 135 75 63 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 602 702 924
Size: 602 100 222
Fragments arranged by size:
602 222 100
BsrDI GCAATG_nn'
Cuts at: 0 482 924
Size: 482 442
BsrFI r'CCGG_y
Cuts at: 0 811 924
Size: 811 113
BstAPI GCAn_nnn'nTGC
Cuts at: 0 675 924
Size: 675 249
BstEII G'GTnAC_C
Cuts at: 0 807 924
Size: 807 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 159 924
Size: 48 111 765
Fragments arranged by size:
765 111 48
BstYI r'GATC_y
Cuts at: 0 85 862 924
Size: 85 777 62
Fragments arranged by size:
777 85 62
BtgI C'CryG_G
Cuts at: 0 467 803 924
Size: 467 336 121
Fragments arranged by size:
467 336 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 255 924
Size: 255 669
EaeI y'GGCC_r
Cuts at: 0 161 504 617 735 924
Size: 161 343 113 118 189
Fragments arranged by size:
343 189 161 118 113
EarI CTCTTCn'nnn_
Cuts at: 0 832 924
Size: 832 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 150 238 924
Size: 150 88 686
Fragments arranged by size:
686 150 88
EcoICRI GAG'CTC
Cuts at: 0 59 924
Size: 59 865
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 45 184 236 532 619 916 924
Size: 24 21 139 52 296 87 297 8
Fragments arranged by size:
297 296 139 87 52 24 21 8
EcoO109I rG'GnC_Cy
Cuts at: 0 19 35 537 608 691 924
Size: 19 16 502 71 83 233
Fragments arranged by size:
502 233 83 71 19 16
FspI TGC'GCA
Cuts at: 0 238 924
Size: 238 686
HaeII r_GCGC'y
Cuts at: 0 803 879 924
Size: 803 76 45
Fragments arranged by size:
803 76 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 516 548 639 671 924
Size: 516 32 91 32 253
Fragments arranged by size:
516 253 91 32 32
KasI G'GCGC_C
Cuts at: 0 875 924
Size: 875 49
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 924
Size: 374 550
MscI TGG'CCA
Cuts at: 0 163 506 619 924
Size: 163 343 113 305
Fragments arranged by size:
343 305 163 113
MslI CAynn'nnrTG
Cuts at: 0 274 763 924
Size: 274 489 161
Fragments arranged by size:
489 274 161
MspA1I CmG'CkG
Cuts at: 0 510 805 924
Size: 510 295 119
Fragments arranged by size:
510 295 119
NarI GG'CG_CC
Cuts at: 0 876 924
Size: 876 48
NcoI C'CATG_G
Cuts at: 0 467 924
Size: 467 457
PasI CC'CwG_GG
Cuts at: 0 696 924
Size: 696 228
PfoI T'CCnGG_A
Cuts at: 0 40 410 924
Size: 40 370 514
Fragments arranged by size:
514 370 40
PpuMI rG'GwC_Cy
Cuts at: 0 19 691 924
Size: 19 672 233
Fragments arranged by size:
672 233 19
PstI C_TGCA'G
Cuts at: 0 713 924
Size: 713 211
PvuII CAG'CTG
Cuts at: 0 510 924
Size: 510 414
SacI G_AGCT'C
Cuts at: 0 61 924
Size: 61 863
SacII CC_GC'GG
Cuts at: 0 806 924
Size: 806 118
SanDI GG'GwC_CC
Cuts at: 0 691 924
Size: 691 233
SfcI C'TryA_G
Cuts at: 0 709 924
Size: 709 215
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 616 924
Size: 616 308
SfoI GGC'GCC
Cuts at: 0 877 924
Size: 877 47
StyI C'CwwG_G
Cuts at: 0 467 924
Size: 467 457
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 136 480 924
Size: 136 344 444
Fragments arranged by size:
444 344 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 924
Size: 736 32 156
Fragments arranged by size:
736 156 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 924
Size: 840 84
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaLI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BglI Bme1580I BmrI BpmI
Bpu10I BsaHI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI
PasI PfoI PpuMI PstI PvuII SacI SacII SanDI
SfcI SfiI SfoI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SapI
SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI