(Circular) (Six-base) MAPSORT of: pFN21ASDA0222.seq Check: 6859 from: 1 to: 8218 pFN21ASDA0222 8218 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2135 2135 Size: 8218 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6028 278 Size: 53 83 186 936 4492 2468 Fragments arranged by size: 4492 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4238 5561 1937 Size: 2301 1323 4594 Fragments arranged by size: 4594 2301 1323 Acc65I G'GTAC_C Cuts at: 5477 5539 5477 Size: 62 8156 AclI AA'CG_TT Cuts at: 1563 6345 6718 1563 Size: 4782 373 3063 Fragments arranged by size: 4782 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2776 2831 4780 5439 6281 7665 7933 Size: 641 503 55 1949 659 842 1384 268 Cuts at: 7933 1632 Size: 1917 Fragments arranged by size: 1949 1917 1384 842 659 641 503 268 55 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8021 AflIII A'CryG_T Cuts at: 1203 3625 7117 8094 1203 Size: 2422 3492 977 1327 Fragments arranged by size: 3492 2422 1327 977 AgeI A'CCGG_T Cuts at: 4768 7049 4768 Size: 2281 5937 AhdI GACnn_n'nnGTC Cuts at: 6947 6947 Size: 8218 AleI CACnn'nnGTG Cuts at: 1166 4538 1166 Size: 3372 4846 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2417 2492 4556 1184 Size: 1233 75 2064 4846 Fragments arranged by size: 4846 2064 1233 75 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2873 2905 2873 Size: 32 8186 AlwNI CAG_nnn'CTG Cuts at: 3181 3968 5050 5961 7533 3181 Size: 787 1082 911 1572 3866 Fragments arranged by size: 3866 1572 1082 911 787 ApaI G_GGCC'C Cuts at: 2709 3150 3450 3685 4475 4734 5368 2709 Size: 441 300 235 790 259 634 5559 Fragments arranged by size: 5559 790 634 441 300 259 235 ApaLI G'TGCA_C Cuts at: 2600 6275 7431 2600 Size: 3675 1156 3387 Fragments arranged by size: 3675 3387 1156 ApoI r'AATT_y Cuts at: 1493 4952 5527 5753 5905 8014 1493 Size: 3459 575 226 152 2109 1697 Fragments arranged by size: 3459 2109 1697 575 226 152 AseI AT'TA_AT Cuts at: 160 1032 6772 160 Size: 872 5740 1606 Fragments arranged by size: 5740 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8218 AvaI C'yCGr_G Cuts at: 1870 1943 2521 2744 2756 3763 4337 4780 4833 Size: 73 578 223 12 1007 574 443 53 Cuts at: 4833 5409 5543 1870 Size: 576 134 4545 Fragments arranged by size: 4545 1007 578 576 574 443 223 134 73 53 12 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5461 5494 5461 Size: 33 8185 BamHI G'GATC_C Cuts at: 5548 5915 5548 Size: 367 7851 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2320 2429 3812 4072 4368 Size: 325 217 627 533 109 1383 260 296 Cuts at: 4368 4540 4657 4771 5125 5477 5539 6994 618 Size: 172 117 114 354 352 62 1455 1842 Fragments arranged by size: 1842 1455 1383 627 533 354 352 325 296 260 217 172 117 114 109 62 BanII G_rGCy'C Cuts at: 1391 2165 2709 2756 2840 2886 3150 3450 3685 Size: 774 544 47 84 46 264 300 235 Cuts at: 3685 3770 3885 4475 4734 5028 5368 5537 1391 Size: 85 115 590 259 294 340 169 4072 Fragments arranged by size: 4072 774 590 544 340 300 294 264 259 235 169 115 85 84 47 46 BbeI G_GCGC'C Cuts at: 2324 2433 3816 4661 5129 2324 Size: 109 1383 845 468 5413 Fragments arranged by size: 5413 1383 845 468 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3801 928 Size: 971 1902 5345 Fragments arranged by size: 5345 1902 971 BbvCI CC'TCA_GC Cuts at: 2054 5051 2054 Size: 2997 5221 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3770 3804 6409 6443 1903 Size: 34 1833 34 2605 34 3678 Fragments arranged by size: 3678 2605 1833 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6110 7320 6110 Size: 1210 7008 BclI T'GATC_A Cuts at: 1548 1548 Size: 8218 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2420 2456 3311 Size: 107 122 71 631 605 748 36 855 Cuts at: 3311 3785 5102 6829 136 Size: 474 1317 1727 1525 Fragments arranged by size: 1727 1525 1317 855 748 631 605 474 122 107 71 36 BglII A'GATC_T Cuts at: 3588 8213 3588 Size: 4625 3593 BlpI GC'TnA_GC Cuts at: 2669 2750 3246 4962 5635 2669 Size: 81 496 1716 673 5252 Fragments arranged by size: 5252 1716 673 496 81 Bme1580I G_kGCm'C Cuts at: 1790 2604 2709 3150 3218 3450 3685 4077 4475 Size: 814 105 441 68 232 235 392 398 Cuts at: 4475 4734 4933 5368 5380 6279 7435 1790 Size: 259 199 435 12 899 1156 2573 Fragments arranged by size: 2573 1156 899 814 441 435 398 392 259 235 232 199 105 68 12 BmgBI CAC'GTC Cuts at: 1318 3361 1318 Size: 2043 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5949 6907 442 Size: 416 53 83 399 24 4532 958 1753 Fragments arranged by size: 4532 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4608 4761 1056 Size: 3552 153 4513 Fragments arranged by size: 4513 3552 153 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3166 3727 4288 4777 5047 5303 3166 Size: 561 561 489 270 256 6081 Fragments arranged by size: 6081 561 561 489 270 256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2609 3158 3871 6878 1239 Size: 1370 549 713 3007 2579 Fragments arranged by size: 3007 2579 1370 713 549 Bpu10I CC'TnA_GC Cuts at: 2054 3014 5051 2054 Size: 960 2037 5221 Fragments arranged by size: 5221 2037 960 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2879 3308 6343 7208 7506 2879 Size: 429 3035 865 298 3591 Fragments arranged by size: 3591 3035 865 429 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3888 5454 6881 882 Size: 617 2389 1566 1427 2219 Fragments arranged by size: 2389 2219 1566 1427 617 BsaAI yAC'GTr Cuts at: 493 1204 2599 5515 6009 493 Size: 711 1395 2916 494 2702 Fragments arranged by size: 2916 2702 1395 711 494 BsaBI GATnn'nnATC Cuts at: 2261 8212 2261 Size: 5951 2267 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2321 2358 2430 3813 Size: 53 83 186 936 788 37 72 1383 Cuts at: 3813 4658 5126 5442 6025 6407 7042 275 Size: 845 468 316 583 382 635 1451 Fragments arranged by size: 1451 1383 936 845 788 635 583 468 382 316 186 83 72 53 37 BsaWI w'CCGG_w Cuts at: 2265 4634 4768 5289 6651 7049 7323 7470 7881 Size: 2369 134 521 1362 398 274 147 411 Cuts at: 7881 2265 Size: 2602 Fragments arranged by size: 2602 2369 1362 521 411 398 274 147 134 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2753 2783 2822 2852 2873 Size: 30 165 30 1350 30 39 30 21 Cuts at: 2873 2903 3701 3731 5301 5331 1178 Size: 30 798 30 1570 30 4065 Fragments arranged by size: 4065 1570 1350 798 165 39 30 30 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2672 3008 3146 3821 4264 4995 4998 1186 Size: 1486 336 138 675 443 731 3 4406 Fragments arranged by size: 4406 1486 731 675 443 336 138 3 BseYI C'CCAG_C Cuts at: 2066 2326 3720 3725 4253 4891 5106 5120 7421 Size: 260 1394 5 528 638 215 14 2301 Cuts at: 7421 7917 2066 Size: 496 2367 Fragments arranged by size: 2367 2301 1394 638 528 496 260 215 14 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2579 2647 2656 3602 3810 1163 Size: 573 843 68 9 946 208 5571 Fragments arranged by size: 5571 946 843 573 208 68 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2086 2869 3129 3204 3315 3516 Size: 275 186 85 783 260 75 111 201 Cuts at: 3516 3879 5668 6429 7457 1540 Size: 363 1789 761 1028 2301 Fragments arranged by size: 2301 1789 1028 783 761 363 275 260 201 186 111 85 75 BsiHKAI G_wGCw'C Cuts at: 2165 2552 2604 3885 4963 5277 6279 6364 7435 Size: 387 52 1281 1078 314 1002 85 1071 Cuts at: 7435 2165 Size: 2948 Fragments arranged by size: 2948 1281 1078 1071 1002 387 314 85 52 BsmI GAATG_Cn' Cuts at: 4505 5729 5822 4505 Size: 1224 93 6901 Fragments arranged by size: 6901 1224 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4259 4259 Size: 8218 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2552 2604 2709 2756 2840 2886 Size: 399 375 387 52 105 47 84 46 Cuts at: 2886 3150 3218 3450 3685 3770 3885 4077 4475 Size: 264 68 232 235 85 115 192 398 Cuts at: 4475 4734 4933 4963 5028 5277 5368 5380 5537 Size: 259 199 30 65 249 91 12 157 Cuts at: 5537 6279 6364 7435 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 398 387 375 264 259 249 235 232 199 192 157 115 105 91 85 85 84 68 65 52 47 46 30 12 BspEI T'CCGG_A Cuts at: 2265 4634 5289 7881 2265 Size: 2369 655 2592 2602 Fragments arranged by size: 2602 2592 2369 655 BspHI T'CATG_A Cuts at: 6107 7837 6107 Size: 1730 6488 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2126 2135 3832 5573 844 Size: 1282 9 1697 1741 3489 Fragments arranged by size: 3489 1741 1697 1282 9 BsrBI CCG'CTC Cuts at: 1394 1823 3468 3510 4118 4461 6105 1394 Size: 429 1645 42 608 343 1644 3507 Fragments arranged by size: 3507 1645 1644 608 429 343 42 BsrDI GCAATG_nn' Cuts at: 65 1248 6713 6887 65 Size: 1183 5465 174 1396 Fragments arranged by size: 5465 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2081 2397 3008 3203 3310 3491 3548 Size: 110 271 316 611 195 107 181 57 Cuts at: 3548 3938 4768 6862 7049 1700 Size: 390 830 2094 187 2869 Fragments arranged by size: 2869 2094 830 611 390 316 271 195 187 181 110 107 57 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8218 BssHII G'CGCG_C Cuts at: 1925 2338 3118 3460 3518 3639 3641 4842 1925 Size: 413 780 342 58 121 2 1201 5301 Fragments arranged by size: 5301 1201 780 413 342 121 58 2 BssSI C'ACGA_G Cuts at: 2093 2300 3211 6278 7290 2093 Size: 207 911 3067 1012 3021 Fragments arranged by size: 3067 3021 1012 911 207 BstAPI GCAn_nnn'nTGC Cuts at: 794 2179 2634 4072 4500 5328 794 Size: 1385 455 1438 428 828 3684 Fragments arranged by size: 3684 1438 1385 828 455 428 BstBI TT'CG_AA Cuts at: 2007 7022 2007 Size: 5015 3203 BstEII G'GTnAC_C Cuts at: 1184 4133 1184 Size: 2949 5269 BstYI r'GATC_y Cuts at: 1972 3588 5548 5915 6300 6317 7769 7855 7867 Size: 1616 1960 367 385 17 1452 86 12 Cuts at: 7867 8213 1972 Size: 346 1977 Fragments arranged by size: 1977 1960 1616 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3188 4968 5360 3188 Size: 1780 392 6046 Fragments arranged by size: 6046 1780 392 BtgI C'CryG_G Cuts at: 513 1065 2022 2848 3452 4389 4400 513 Size: 552 957 826 604 937 11 4331 Fragments arranged by size: 4331 957 937 826 604 552 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2950 3617 4813 487 Size: 683 56 1724 667 1196 3892 Fragments arranged by size: 3892 1724 1196 683 667 56 BtsI GCAGTG_nn' Cuts at: 5734 6528 6548 5734 Size: 794 20 7404 Fragments arranged by size: 7404 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8218 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8183 DraI TTT'AAA Cuts at: 5522 5522 Size: 8218 DraIII CAC_nnn'GTG Cuts at: 2098 3701 5386 2098 Size: 1603 1685 4930 Fragments arranged by size: 4930 1685 1603 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7225 809 Size: 507 5909 1802 Fragments arranged by size: 5909 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2782 2866 Size: 54 1425 50 275 18 253 699 84 Cuts at: 2866 3126 3201 3285 3303 3312 3351 3493 3513 Size: 260 75 84 18 9 39 142 20 Cuts at: 3513 3550 3777 4699 4719 4737 4854 5061 5163 Size: 37 227 922 20 18 117 207 102 Cuts at: 5163 5303 5353 5665 6554 8183 8 Size: 140 50 312 889 1629 43 Fragments arranged by size: 1629 1425 922 889 699 312 275 260 253 227 207 142 140 117 102 84 84 75 54 50 50 43 39 37 20 20 18 18 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2083 2866 3126 3201 3312 3513 5665 Size: 275 271 783 260 75 111 201 2152 Cuts at: 5665 1537 Size: 4090 Fragments arranged by size: 4090 2152 783 275 271 260 201 111 75 EarI CTCTTCn'nnn_ Cuts at: 1353 3858 4579 6148 1353 Size: 2505 721 1569 3423 Fragments arranged by size: 3423 2505 1569 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3155 3730 6805 7179 7325 387 Size: 1064 138 1566 575 3075 374 146 1280 Fragments arranged by size: 3075 1566 1280 1064 575 374 146 138 EcoICRI GAG'CTC Cuts at: 2163 3883 2163 Size: 1720 6498 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2609 2776 2831 3158 3871 4780 Size: 393 641 336 167 55 327 713 909 Cuts at: 4780 5439 6281 6878 7665 7933 1239 Size: 659 842 597 787 268 1524 Fragments arranged by size: 1524 909 842 787 713 659 641 597 393 336 327 268 167 55 EcoNI CCTnn'n_nnAGG Cuts at: 3023 3828 5240 3023 Size: 805 1412 6001 Fragments arranged by size: 6001 1412 805 EcoO109I rG'GnC_Cy Cuts at: 2063 2350 2412 2437 2448 2584 2705 3146 3147 Size: 287 62 25 11 136 121 441 1 Cuts at: 3147 3333 3446 3447 3478 4342 4472 4640 4730 Size: 186 113 1 31 864 130 168 90 Cuts at: 4730 4731 4885 5364 2063 Size: 1 154 479 4917 Fragments arranged by size: 4917 864 479 441 287 186 168 154 136 130 121 113 90 62 31 25 11 1 1 1 EcoRI G'AATT_C Cuts at: 5527 5905 5527 Size: 378 7840 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3184 4590 3184 Size: 1406 6812 FseI GG_CCGG'CC Cuts at: 2085 2085 Size: 8218 FspI TGC'GCA Cuts at: 2183 6724 2183 Size: 4541 3677 HaeII r_GCGC'y Cuts at: 1678 2324 2385 2433 3350 3816 4199 4661 4809 Size: 646 61 48 917 466 383 462 148 Cuts at: 4809 5004 5129 5185 7365 1678 Size: 195 125 56 2180 2531 Fragments arranged by size: 2531 2180 917 646 466 462 383 195 148 125 61 56 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3494 3526 3701 3733 3908 Size: 32 135 32 1922 32 175 32 175 Cuts at: 3908 3940 5238 5270 5301 5333 5479 5511 6859 Size: 32 1298 32 31 32 146 32 1348 Cuts at: 6859 6891 6933 6965 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1922 1348 1298 175 175 146 135 42 32 32 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 3262 4239 5562 5808 669 Size: 988 281 1324 977 1323 246 3079 Fragments arranged by size: 3079 1324 1323 988 977 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8218 HpaI GTT'AAC Cuts at: 5808 5808 Size: 8218 KasI G'GCGC_C Cuts at: 2320 2429 3812 4657 5125 2320 Size: 109 1383 845 468 5413 Fragments arranged by size: 5413 1383 845 468 109 KpnI G_GTAC'C Cuts at: 5481 5543 5481 Size: 62 8156 MfeI C'AATT_G Cuts at: 5817 5817 Size: 8218 MluI A'CGCG_T Cuts at: 3625 8094 3625 Size: 4469 3749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5250 7332 7516 1305 Size: 3945 2082 184 2007 Fragments arranged by size: 3945 2082 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2784 3287 4739 5063 10 Size: 54 1425 343 952 503 1452 324 3165 Fragments arranged by size: 3165 1452 1425 952 503 343 324 54 MslI CAynn'nnrTG Cuts at: 518 1166 4538 6176 6535 6694 518 Size: 648 3372 1638 359 159 2042 Fragments arranged by size: 3372 2042 1638 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2326 2466 2540 2715 3283 3454 3687 3752 Size: 720 140 74 175 568 171 233 65 Cuts at: 3752 3849 4380 4895 5259 5634 6311 7459 7704 Size: 97 531 515 364 375 677 1148 245 Cuts at: 7704 1606 Size: 2120 Fragments arranged by size: 2120 1148 720 677 568 531 515 375 364 245 233 175 171 140 97 74 65 NaeI GCC'GGC Cuts at: 2083 3010 3205 3312 3493 2083 Size: 927 195 107 181 6808 Fragments arranged by size: 6808 927 195 181 107 NarI GG'CG_CC Cuts at: 2321 2430 3813 4658 5126 2321 Size: 109 1383 845 468 5413 Fragments arranged by size: 5413 1383 845 468 109 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6709 Fragments arranged by size: 6709 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8218 NgoMIV G'CCGG_C Cuts at: 2081 3008 3203 3310 3491 2081 Size: 927 195 107 181 6808 Fragments arranged by size: 6808 927 195 181 107 NheI G'CTAG_C Cuts at: 1052 4604 4757 1052 Size: 3552 153 4513 Fragments arranged by size: 4513 3552 153 NotI GC'GGCC_GC Cuts at: 5665 5665 Size: 8218 NspI r_CATG'y Cuts at: 1132 1229 2182 2517 4612 5374 5576 7121 1132 Size: 97 953 335 2095 762 202 1545 2229 Fragments arranged by size: 2229 2095 1545 953 762 335 202 97 PasI CC'CwG_GG Cuts at: 1348 4582 5264 1348 Size: 3234 682 4302 Fragments arranged by size: 4302 3234 682 PciI A'CATG_T Cuts at: 7117 7117 Size: 8218 PflMI CCAn_nnn'nTGG Cuts at: 1108 2098 1108 Size: 990 7228 PfoI T'CCnGG_A Cuts at: 2988 4454 2988 Size: 1466 6752 PmeI GTTT'AAAC Cuts at: 5522 5522 Size: 8218 PmlI CAC'GTG Cuts at: 2599 2599 Size: 8218 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2399 2431 2435 2467 2873 2905 6287 6319 7800 Size: 32 4 32 406 32 3382 32 1481 Cuts at: 7800 7832 2399 Size: 32 2785 Fragments arranged by size: 3382 2785 1481 406 32 32 32 32 32 4 PpuMI rG'GwC_Cy Cuts at: 2437 2584 3478 4342 4640 4885 2437 Size: 147 894 864 298 245 5770 Fragments arranged by size: 5770 894 864 298 245 147 PshAI GACnn'nnGTC Cuts at: 1613 3900 1613 Size: 2287 5931 PsiI TTA'TAA Cuts at: 5788 5788 Size: 8218 PspOMI G'GGCC_C Cuts at: 2705 3146 3446 3681 4471 4730 5364 2705 Size: 441 300 235 790 259 634 5559 Fragments arranged by size: 5559 790 634 441 300 259 235 PstI C_TGCA'G Cuts at: 830 4144 4284 4319 5570 830 Size: 3314 140 35 1251 3478 Fragments arranged by size: 3478 3314 1251 140 35 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8218 PvuII CAG'CTG Cuts at: 3283 4380 3283 Size: 1097 7121 SacI G_AGCT'C Cuts at: 2165 3885 2165 Size: 1720 6498 SacII CC_GC'GG Cuts at: 3455 3455 Size: 8218 SalI G'TCGA_C Cuts at: 1936 4237 5560 1936 Size: 2301 1323 4594 Fragments arranged by size: 4594 2301 1323 SanDI GG'GwC_CC Cuts at: 3478 4885 3478 Size: 1407 6811 SapI GCTCTTCn'nnn_ Cuts at: 4579 4579 Size: 8218 SbfI CC_TGCA'GG Cuts at: 4284 5570 4284 Size: 1286 6932 ScaI AGT'ACT Cuts at: 6466 8178 6466 Size: 1712 6506 SexAI A'CCwGG_T Cuts at: 2801 2801 Size: 8218 SfcI C'TryA_G Cuts at: 826 1045 4140 4280 4315 5566 6701 7382 7573 Size: 219 3095 140 35 1251 1135 681 191 Cuts at: 7573 826 Size: 1471 Fragments arranged by size: 3095 1471 1251 1135 681 219 191 140 35 SfiI GGCCn_nnn'nGGCC Cuts at: 2420 2456 3311 5102 2420 Size: 36 855 1791 5536 Fragments arranged by size: 5536 1791 855 36 SfoI GGC'GCC Cuts at: 2322 2431 3814 4659 5127 2322 Size: 109 1383 845 468 5413 Fragments arranged by size: 5413 1383 845 468 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8218 SgrAI Cr'CCGG_yG Cuts at: 1700 3938 1700 Size: 2238 5980 SmaI CCC'GGG Cuts at: 1872 2523 2758 3765 4782 4835 5545 1872 Size: 651 235 1007 1017 53 710 4545 Fragments arranged by size: 4545 1017 1007 710 651 235 53 SmlI C'TyrA_G Cuts at: 820 1017 1943 2894 3323 6322 7223 7485 820 Size: 197 926 951 429 2999 901 262 1553 Fragments arranged by size: 2999 1553 951 926 901 429 262 197 SnaBI TAC'GTA Cuts at: 493 5515 6009 493 Size: 5022 494 2702 Fragments arranged by size: 5022 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8218 SphI G_CATG'C Cuts at: 1132 2517 4612 5576 1132 Size: 1385 2095 964 3774 Fragments arranged by size: 3774 2095 1385 964 SrfI GCCC'GGGC Cuts at: 4835 4835 Size: 8218 SspI AAT'ATT Cuts at: 5 52 6142 5 Size: 47 6090 2081 Fragments arranged by size: 6090 2081 47 StuI AGG'CCT Cuts at: 1517 2723 2789 3275 1517 Size: 1206 66 486 6460 Fragments arranged by size: 6460 1206 486 66 StyI C'CwwG_G Cuts at: 513 1065 2022 2532 3948 5657 513 Size: 552 957 510 1416 1709 3074 Fragments arranged by size: 3074 1709 1416 957 552 510 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2318 3893 6245 6262 6415 6600 7045 Size: 1255 738 1575 2352 17 153 185 445 Cuts at: 7045 325 Size: 1498 Fragments arranged by size: 2352 1575 1498 1255 738 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6464 8176 96 Size: 274 80 33 51 1443 4487 1712 138 Fragments arranged by size: 4487 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2219 2251 2753 2785 2822 2854 3225 3257 8178 Size: 32 502 32 37 32 371 32 4921 Cuts at: 8178 8210 2219 Size: 32 2227 Fragments arranged by size: 4921 2227 502 371 37 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3257 1316 Size: 1941 6277 XbaI T'CTAG_A Cuts at: 4004 5554 4004 Size: 1550 6668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2099 2953 2980 4709 2099 Size: 854 27 1729 5608 Fragments arranged by size: 5608 1729 854 27 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8218 XmaI C'CCGG_G Cuts at: 1870 2521 2756 3763 4780 4833 5543 1870 Size: 651 235 1007 1017 53 710 4545 Fragments arranged by size: 4545 1017 1007 710 651 235 53 XmnI GAAnn'nnTTC Cuts at: 6347 6347 Size: 8218 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6026 276 Size: 53 83 186 936 4492 2468 Fragments arranged by size: 4492 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BsiWI BstXI BstZ17I EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII SwaI