Restriction map for pFN21ASDA0222
 (Circular) (Six-base) MAPSORT of: pFN21ASDA0222.seq  Check: 6859  from: 1  to: 8218



pFN21ASDA0222  8218 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2135    2135 
   Size:       8218
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    6028     278 
   Size:         53      83     186     936    4492    2468

  Fragments arranged by size: 

                4492    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4238    5561    1937 
   Size:       2301    1323    4594

  Fragments arranged by size: 

                4594    2301    1323
 
Acc65I G'GTAC_C

Cuts at:   5477    5539    5477 
   Size:         62    8156
 
AclI AA'CG_TT

Cuts at:   1563    6345    6718    1563 
   Size:       4782     373    3063

  Fragments arranged by size: 

                4782    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2273    2776    2831    4780    5439    6281    7665    7933 
   Size:        641     503      55    1949     659     842    1384     268

Cuts at:   7933    1632 
   Size:       1917

  Fragments arranged by size: 

                1949    1917    1384     842     659     641     503     268
                  55
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    8021
 
AflIII A'CryG_T

Cuts at:   1203    3625    7117    8094    1203 
   Size:       2422    3492     977    1327

  Fragments arranged by size: 

                3492    2422    1327     977
 
AgeI A'CCGG_T

Cuts at:   4768    7049    4768 
   Size:       2281    5937
 
AhdI GACnn_n'nnGTC

Cuts at:   6947    6947 
   Size:       8218
 
AleI CACnn'nnGTG

Cuts at:   1166    4538    1166 
   Size:       3372    4846
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2417    2492    4556    1184 
   Size:       1233      75    2064    4846

  Fragments arranged by size: 

                4846    2064    1233      75
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2873    2905    2873 
   Size:         32    8186
 
AlwNI CAG_nnn'CTG

Cuts at:   3181    3968    5050    5961    7533    3181 
   Size:        787    1082     911    1572    3866

  Fragments arranged by size: 

                3866    1572    1082     911     787
 
ApaI G_GGCC'C

Cuts at:   2709    3150    3450    3685    4475    4734    5368    2709 
   Size:        441     300     235     790     259     634    5559

  Fragments arranged by size: 

                5559     790     634     441     300     259     235
 
ApaLI G'TGCA_C

Cuts at:   2600    6275    7431    2600 
   Size:       3675    1156    3387

  Fragments arranged by size: 

                3675    3387    1156
 
ApoI r'AATT_y

Cuts at:   1493    4952    5527    5753    5905    8014    1493 
   Size:       3459     575     226     152    2109    1697

  Fragments arranged by size: 

                3459    2109    1697     575     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    6772     160 
   Size:        872    5740    1606

  Fragments arranged by size: 

                5740    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       8218
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2521    2744    2756    3763    4337    4780    4833 
   Size:         73     578     223      12    1007     574     443      53

Cuts at:   4833    5409    5543    1870 
   Size:        576     134    4545

  Fragments arranged by size: 

                4545    1007     578     576     574     443     223     134
                  73      53      12
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   5461    5494    5461 
   Size:         33    8185
 
BamHI G'GATC_C

Cuts at:   5548    5915    5548 
   Size:        367    7851
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2320    2429    3812    4072    4368 
   Size:        325     217     627     533     109    1383     260     296

Cuts at:   4368    4540    4657    4771    5125    5477    5539    6994     618 
   Size:        172     117     114     354     352      62    1455    1842

  Fragments arranged by size: 

                1842    1455    1383     627     533     354     352     325
                 296     260     217     172     117     114     109      62
            
 
BanII G_rGCy'C

Cuts at:   1391    2165    2709    2756    2840    2886    3150    3450    3685 
   Size:        774     544      47      84      46     264     300     235

Cuts at:   3685    3770    3885    4475    4734    5028    5368    5537    1391 
   Size:         85     115     590     259     294     340     169    4072

  Fragments arranged by size: 

                4072     774     590     544     340     300     294     264
                 259     235     169     115      85      84      47      46
            
 
BbeI G_GCGC'C

Cuts at:   2324    2433    3816    4661    5129    2324 
   Size:        109    1383     845     468    5413

  Fragments arranged by size: 

                5413    1383     845     468     109
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3801     928 
   Size:        971    1902    5345

  Fragments arranged by size: 

                5345    1902     971
 
BbvCI CC'TCA_GC

Cuts at:   2054    5051    2054 
   Size:       2997    5221
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3770    3804    6409    6443    1903 
   Size:         34    1833      34    2605      34    3678

  Fragments arranged by size: 

                3678    2605    1833      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   6110    7320    6110 
   Size:       1210    7008
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       8218
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2420    2456    3311 
   Size:        107     122      71     631     605     748      36     855

Cuts at:   3311    3785    5102    6829     136 
   Size:        474    1317    1727    1525

  Fragments arranged by size: 

                1727    1525    1317     855     748     631     605     474
                 122     107      71      36
 
BglII A'GATC_T

Cuts at:   3588    8213    3588 
   Size:       4625    3593
 
BlpI GC'TnA_GC

Cuts at:   2669    2750    3246    4962    5635    2669 
   Size:         81     496    1716     673    5252

  Fragments arranged by size: 

                5252    1716     673     496      81
 
Bme1580I G_kGCm'C

Cuts at:   1790    2604    2709    3150    3218    3450    3685    4077    4475 
   Size:        814     105     441      68     232     235     392     398

Cuts at:   4475    4734    4933    5368    5380    6279    7435    1790 
   Size:        259     199     435      12     899    1156    2573

  Fragments arranged by size: 

                2573    1156     899     814     441     435     398     392
                 259     235     232     199     105      68      12
 
BmgBI CAC'GTC

Cuts at:   1318    3361    1318 
   Size:       2043    6175
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    5949    6907     442 
   Size:        416      53      83     399      24    4532     958    1753

  Fragments arranged by size: 

                4532    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    4608    4761    1056 
   Size:       3552     153    4513

  Fragments arranged by size: 

                4513    3552     153
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3166    3727    4288    4777    5047    5303    3166 
   Size:        561     561     489     270     256    6081

  Fragments arranged by size: 

                6081     561     561     489     270     256
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2609    3158    3871    6878    1239 
   Size:       1370     549     713    3007    2579

  Fragments arranged by size: 

                3007    2579    1370     713     549
 
Bpu10I CC'TnA_GC

Cuts at:   2054    3014    5051    2054 
   Size:        960    2037    5221

  Fragments arranged by size: 

                5221    2037     960
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2879    3308    6343    7208    7506    2879 
   Size:        429    3035     865     298    3591

  Fragments arranged by size: 

                3591    3035     865     429     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3888    5454    6881     882 
   Size:        617    2389    1566    1427    2219

  Fragments arranged by size: 

                2389    2219    1566    1427     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2599    5515    6009     493 
   Size:        711    1395    2916     494    2702

  Fragments arranged by size: 

                2916    2702    1395     711     494
 
BsaBI GATnn'nnATC

Cuts at:   2261    8212    2261 
   Size:       5951    2267
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2321    2358    2430    3813 
   Size:         53      83     186     936     788      37      72    1383

Cuts at:   3813    4658    5126    5442    6025    6407    7042     275 
   Size:        845     468     316     583     382     635    1451

  Fragments arranged by size: 

                1451    1383     936     845     788     635     583     468
                 382     316     186      83      72      53      37
 
BsaWI w'CCGG_w

Cuts at:   2265    4634    4768    5289    6651    7049    7323    7470    7881 
   Size:       2369     134     521    1362     398     274     147     411

Cuts at:   7881    2265 
   Size:       2602

  Fragments arranged by size: 

                2602    2369    1362     521     411     398     274     147
                 134
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2753    2783    2822    2852    2873 
   Size:         30     165      30    1350      30      39      30      21

Cuts at:   2873    2903    3701    3731    5301    5331    1178 
   Size:         30     798      30    1570      30    4065

  Fragments arranged by size: 

                4065    1570    1350     798     165      39      30      30
                  30      30      30      30      30      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2672    3008    3146    3821    4264    4995    4998    1186 
   Size:       1486     336     138     675     443     731       3    4406

  Fragments arranged by size: 

                4406    1486     731     675     443     336     138       3
            
 
BseYI C'CCAG_C

Cuts at:   2066    2326    3720    3725    4253    4891    5106    5120    7421 
   Size:        260    1394       5     528     638     215      14    2301

Cuts at:   7421    7917    2066 
   Size:        496    2367

  Fragments arranged by size: 

                2367    2301    1394     638     528     496     260     215
                  14       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2579    2647    2656    3602    3810    1163 
   Size:        573     843      68       9     946     208    5571

  Fragments arranged by size: 

                5571     946     843     573     208      68       9
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2086    2869    3129    3204    3315    3516 
   Size:        275     186      85     783     260      75     111     201

Cuts at:   3516    3879    5668    6429    7457    1540 
   Size:        363    1789     761    1028    2301

  Fragments arranged by size: 

                2301    1789    1028     783     761     363     275     260
                 201     186     111      85      75
 
BsiHKAI G_wGCw'C

Cuts at:   2165    2552    2604    3885    4963    5277    6279    6364    7435 
   Size:        387      52    1281    1078     314    1002      85    1071

Cuts at:   7435    2165 
   Size:       2948

  Fragments arranged by size: 

                2948    1281    1078    1071    1002     387     314      85
                  52
 
BsmI GAATG_Cn'

Cuts at:   4505    5729    5822    4505 
   Size:       1224      93    6901

  Fragments arranged by size: 

                6901    1224      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   4259    4259 
   Size:       8218
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2165    2552    2604    2709    2756    2840    2886 
   Size:        399     375     387      52     105      47      84      46

Cuts at:   2886    3150    3218    3450    3685    3770    3885    4077    4475 
   Size:        264      68     232     235      85     115     192     398

Cuts at:   4475    4734    4933    4963    5028    5277    5368    5380    5537 
   Size:        259     199      30      65     249      91      12     157

Cuts at:   5537    6279    6364    7435    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     398     387     375     264
                 259     249     235     232     199     192     157     115
                 105      91      85      85      84      68      65      52
                  47      46      30      12
 
BspEI T'CCGG_A

Cuts at:   2265    4634    5289    7881    2265 
   Size:       2369     655    2592    2602

  Fragments arranged by size: 

                2602    2592    2369     655
 
BspHI T'CATG_A

Cuts at:   6107    7837    6107 
   Size:       1730    6488
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2126    2135    3832    5573     844 
   Size:       1282       9    1697    1741    3489

  Fragments arranged by size: 

                3489    1741    1697    1282       9
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3468    3510    4118    4461    6105    1394 
   Size:        429    1645      42     608     343    1644    3507

  Fragments arranged by size: 

                3507    1645    1644     608     429     343      42
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    6713    6887      65 
   Size:       1183    5465     174    1396

  Fragments arranged by size: 

                5465    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2081    2397    3008    3203    3310    3491    3548 
   Size:        110     271     316     611     195     107     181      57

Cuts at:   3548    3938    4768    6862    7049    1700 
   Size:        390     830    2094     187    2869

  Fragments arranged by size: 

                2869    2094     830     611     390     316     271     195
                 187     181     110     107      57
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       8218
 
BssHII G'CGCG_C

Cuts at:   1925    2338    3118    3460    3518    3639    3641    4842    1925 
   Size:        413     780     342      58     121       2    1201    5301

  Fragments arranged by size: 

                5301    1201     780     413     342     121      58       2
            
 
BssSI C'ACGA_G

Cuts at:   2093    2300    3211    6278    7290    2093 
   Size:        207     911    3067    1012    3021

  Fragments arranged by size: 

                3067    3021    1012     911     207
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2179    2634    4072    4500    5328     794 
   Size:       1385     455    1438     428     828    3684

  Fragments arranged by size: 

                3684    1438    1385     828     455     428
 
BstBI TT'CG_AA

Cuts at:   2007    7022    2007 
   Size:       5015    3203
 
BstEII G'GTnAC_C

Cuts at:   1184    4133    1184 
   Size:       2949    5269
 
BstYI r'GATC_y

Cuts at:   1972    3588    5548    5915    6300    6317    7769    7855    7867 
   Size:       1616    1960     367     385      17    1452      86      12

Cuts at:   7867    8213    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1960    1616    1452     385     367     346      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3188    4968    5360    3188 
   Size:       1780     392    6046

  Fragments arranged by size: 

                6046    1780     392
 
BtgI C'CryG_G

Cuts at:    513    1065    2022    2848    3452    4389    4400     513 
   Size:        552     957     826     604     937      11    4331

  Fragments arranged by size: 

                4331     957     937     826     604     552      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2950    3617    4813     487 
   Size:        683      56    1724     667    1196    3892

  Fragments arranged by size: 

                3892    1724    1196     683     667      56
 
BtsI GCAGTG_nn'

Cuts at:   5734    6528    6548    5734 
   Size:        794      20    7404

  Fragments arranged by size: 

                7404     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       8218
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    8183
 
DraI TTT'AAA

Cuts at:   5522    5522 
   Size:       8218
 
DraIII CAC_nnn'GTG

Cuts at:   2098    3701    5386    2098 
   Size:       1603    1685    4930

  Fragments arranged by size: 

                4930    1685    1603
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    7225     809 
   Size:        507    5909    1802

  Fragments arranged by size: 

                5909    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2083    2782    2866 
   Size:         54    1425      50     275      18     253     699      84

Cuts at:   2866    3126    3201    3285    3303    3312    3351    3493    3513 
   Size:        260      75      84      18       9      39     142      20

Cuts at:   3513    3550    3777    4699    4719    4737    4854    5061    5163 
   Size:         37     227     922      20      18     117     207     102

Cuts at:   5163    5303    5353    5665    6554    8183       8 
   Size:        140      50     312     889    1629      43

  Fragments arranged by size: 

                1629    1425     922     889     699     312     275     260
                 253     227     207     142     140     117     102      84
                  84      75      54      50      50      43      39      37
                  20      20      18      18      18       9
 
EagI C'GGCC_G

Cuts at:   1537    1812    2083    2866    3126    3201    3312    3513    5665 
   Size:        275     271     783     260      75     111     201    2152

Cuts at:   5665    1537 
   Size:       4090

  Fragments arranged by size: 

                4090    2152     783     275     271     260     201     111
                  75
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3858    4579    6148    1353 
   Size:       2505     721    1569    3423

  Fragments arranged by size: 

                3423    2505    1569     721
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3155    3730    6805    7179    7325     387 
   Size:       1064     138    1566     575    3075     374     146    1280

  Fragments arranged by size: 

                3075    1566    1280    1064     575     374     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   2163    3883    2163 
   Size:       1720    6498
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2273    2609    2776    2831    3158    3871    4780 
   Size:        393     641     336     167      55     327     713     909

Cuts at:   4780    5439    6281    6878    7665    7933    1239 
   Size:        659     842     597     787     268    1524

  Fragments arranged by size: 

                1524     909     842     787     713     659     641     597
                 393     336     327     268     167      55
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3023    3828    5240    3023 
   Size:        805    1412    6001

  Fragments arranged by size: 

                6001    1412     805
 
EcoO109I rG'GnC_Cy

Cuts at:   2063    2350    2412    2437    2448    2584    2705    3146    3147 
   Size:        287      62      25      11     136     121     441       1

Cuts at:   3147    3333    3446    3447    3478    4342    4472    4640    4730 
   Size:        186     113       1      31     864     130     168      90

Cuts at:   4730    4731    4885    5364    2063 
   Size:          1     154     479    4917

  Fragments arranged by size: 

                4917     864     479     441     287     186     168     154
                 136     130     121     113      90      62      31      25
                  11       1       1       1
 
EcoRI G'AATT_C

Cuts at:   5527    5905    5527 
   Size:        378    7840
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3184    4590    3184 
   Size:       1406    6812
 
FseI GG_CCGG'CC

Cuts at:   2085    2085 
   Size:       8218
 
FspI TGC'GCA

Cuts at:   2183    6724    2183 
   Size:       4541    3677
 
HaeII r_GCGC'y

Cuts at:   1678    2324    2385    2433    3350    3816    4199    4661    4809 
   Size:        646      61      48     917     466     383     462     148

Cuts at:   4809    5004    5129    5185    7365    1678 
   Size:        195     125      56    2180    2531

  Fragments arranged by size: 

                2531    2180     917     646     466     462     383     195
                 148     125      61      56      48
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3494    3526    3701    3733    3908 
   Size:         32     135      32    1922      32     175      32     175

Cuts at:   3908    3940    5238    5270    5301    5333    5479    5511    6859 
   Size:         32    1298      32      31      32     146      32    1348

Cuts at:   6859    6891    6933    6965    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1922    1348    1298     175     175     146     135
                  42      32      32      32      32      32      32      32
                  32      32      32      31
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3262    4239    5562    5808     669 
   Size:        988     281    1324     977    1323     246    3079

  Fragments arranged by size: 

                3079    1324    1323     988     977     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       8218
 
HpaI GTT'AAC

Cuts at:   5808    5808 
   Size:       8218
 
KasI G'GCGC_C

Cuts at:   2320    2429    3812    4657    5125    2320 
   Size:        109    1383     845     468    5413

  Fragments arranged by size: 

                5413    1383     845     468     109
 
KpnI G_GTAC'C

Cuts at:   5481    5543    5481 
   Size:         62    8156
 
MfeI C'AATT_G

Cuts at:   5817    5817 
   Size:       8218
 
MluI A'CGCG_T

Cuts at:   3625    8094    3625 
   Size:       4469    3749
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5250    7332    7516    1305 
   Size:       3945    2082     184    2007

  Fragments arranged by size: 

                3945    2082    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2784    3287    4739    5063      10 
   Size:         54    1425     343     952     503    1452     324    3165

  Fragments arranged by size: 

                3165    1452    1425     952     503     343     324      54
            
 
MslI CAynn'nnrTG

Cuts at:    518    1166    4538    6176    6535    6694     518 
   Size:        648    3372    1638     359     159    2042

  Fragments arranged by size: 

                3372    2042    1638     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2326    2466    2540    2715    3283    3454    3687    3752 
   Size:        720     140      74     175     568     171     233      65

Cuts at:   3752    3849    4380    4895    5259    5634    6311    7459    7704 
   Size:         97     531     515     364     375     677    1148     245

Cuts at:   7704    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     720     677     568     531     515     375
                 364     245     233     175     171     140      97      74
                  65
 
NaeI GCC'GGC

Cuts at:   2083    3010    3205    3312    3493    2083 
   Size:        927     195     107     181    6808

  Fragments arranged by size: 

                6808     927     195     181     107
 
NarI GG'CG_CC

Cuts at:   2321    2430    3813    4658    5126    2321 
   Size:        109    1383     845     468    5413

  Fragments arranged by size: 

                5413    1383     845     468     109
 
NcoI C'CATG_G

Cuts at:    513    1065    2022     513 
   Size:        552     957    6709

  Fragments arranged by size: 

                6709     957     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       8218
 
NgoMIV G'CCGG_C

Cuts at:   2081    3008    3203    3310    3491    2081 
   Size:        927     195     107     181    6808

  Fragments arranged by size: 

                6808     927     195     181     107
 
NheI G'CTAG_C

Cuts at:   1052    4604    4757    1052 
   Size:       3552     153    4513

  Fragments arranged by size: 

                4513    3552     153
 
NotI GC'GGCC_GC

Cuts at:   5665    5665 
   Size:       8218
 
NspI r_CATG'y

Cuts at:   1132    1229    2182    2517    4612    5374    5576    7121    1132 
   Size:         97     953     335    2095     762     202    1545    2229

  Fragments arranged by size: 

                2229    2095    1545     953     762     335     202      97
            
 
PasI CC'CwG_GG

Cuts at:   1348    4582    5264    1348 
   Size:       3234     682    4302

  Fragments arranged by size: 

                4302    3234     682
 
PciI A'CATG_T

Cuts at:   7117    7117 
   Size:       8218
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2098    1108 
   Size:        990    7228
 
PfoI T'CCnGG_A

Cuts at:   2988    4454    2988 
   Size:       1466    6752
 
PmeI GTTT'AAAC

Cuts at:   5522    5522 
   Size:       8218
 
PmlI CAC'GTG

Cuts at:   2599    2599 
   Size:       8218
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2399    2431    2435    2467    2873    2905    6287    6319    7800 
   Size:         32       4      32     406      32    3382      32    1481

Cuts at:   7800    7832    2399 
   Size:         32    2785

  Fragments arranged by size: 

                3382    2785    1481     406      32      32      32      32
                  32       4
 
PpuMI rG'GwC_Cy

Cuts at:   2437    2584    3478    4342    4640    4885    2437 
   Size:        147     894     864     298     245    5770

  Fragments arranged by size: 

                5770     894     864     298     245     147
 
PshAI GACnn'nnGTC

Cuts at:   1613    3900    1613 
   Size:       2287    5931
 
PsiI TTA'TAA

Cuts at:   5788    5788 
   Size:       8218
 
PspOMI G'GGCC_C

Cuts at:   2705    3146    3446    3681    4471    4730    5364    2705 
   Size:        441     300     235     790     259     634    5559

  Fragments arranged by size: 

                5559     790     634     441     300     259     235
 
PstI C_TGCA'G

Cuts at:    830    4144    4284    4319    5570     830 
   Size:       3314     140      35    1251    3478

  Fragments arranged by size: 

                3478    3314    1251     140      35
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       8218
 
PvuII CAG'CTG

Cuts at:   3283    4380    3283 
   Size:       1097    7121
 
SacI G_AGCT'C

Cuts at:   2165    3885    2165 
   Size:       1720    6498
 
SacII CC_GC'GG

Cuts at:   3455    3455 
   Size:       8218
 
SalI G'TCGA_C

Cuts at:   1936    4237    5560    1936 
   Size:       2301    1323    4594

  Fragments arranged by size: 

                4594    2301    1323
 
SanDI GG'GwC_CC

Cuts at:   3478    4885    3478 
   Size:       1407    6811
 
SapI GCTCTTCn'nnn_

Cuts at:   4579    4579 
   Size:       8218
 
SbfI CC_TGCA'GG

Cuts at:   4284    5570    4284 
   Size:       1286    6932
 
ScaI AGT'ACT

Cuts at:   6466    8178    6466 
   Size:       1712    6506
 
SexAI A'CCwGG_T

Cuts at:   2801    2801 
   Size:       8218
 
SfcI C'TryA_G

Cuts at:    826    1045    4140    4280    4315    5566    6701    7382    7573 
   Size:        219    3095     140      35    1251    1135     681     191

Cuts at:   7573     826 
   Size:       1471

  Fragments arranged by size: 

                3095    1471    1251    1135     681     219     191     140
                  35
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2420    2456    3311    5102    2420 
   Size:         36     855    1791    5536

  Fragments arranged by size: 

                5536    1791     855      36
 
SfoI GGC'GCC

Cuts at:   2322    2431    3814    4659    5127    2322 
   Size:        109    1383     845     468    5413

  Fragments arranged by size: 

                5413    1383     845     468     109
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       8218
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    3938    1700 
   Size:       2238    5980
 
SmaI CCC'GGG

Cuts at:   1872    2523    2758    3765    4782    4835    5545    1872 
   Size:        651     235    1007    1017      53     710    4545

  Fragments arranged by size: 

                4545    1017    1007     710     651     235      53
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2894    3323    6322    7223    7485     820 
   Size:        197     926     951     429    2999     901     262    1553

  Fragments arranged by size: 

                2999    1553     951     926     901     429     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    5515    6009     493 
   Size:       5022     494    2702

  Fragments arranged by size: 

                5022    2702     494
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       8218
 
SphI G_CATG'C

Cuts at:   1132    2517    4612    5576    1132 
   Size:       1385    2095     964    3774

  Fragments arranged by size: 

                3774    2095    1385     964
 
SrfI GCCC'GGGC

Cuts at:   4835    4835 
   Size:       8218
 
SspI AAT'ATT

Cuts at:      5      52    6142       5 
   Size:         47    6090    2081

  Fragments arranged by size: 

                6090    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2723    2789    3275    1517 
   Size:       1206      66     486    6460

  Fragments arranged by size: 

                6460    1206     486      66
 
StyI C'CwwG_G

Cuts at:    513    1065    2022    2532    3948    5657     513 
   Size:        552     957     510    1416    1709    3074

  Fragments arranged by size: 

                3074    1709    1416     957     552     510
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2318    3893    6245    6262    6415    6600    7045 
   Size:       1255     738    1575    2352      17     153     185     445

Cuts at:   7045     325 
   Size:       1498

  Fragments arranged by size: 

                2352    1575    1498    1255     738     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    6464    8176      96 
   Size:        274      80      33      51    1443    4487    1712     138

  Fragments arranged by size: 

                4487    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2219    2251    2753    2785    2822    2854    3225    3257    8178 
   Size:         32     502      32      37      32     371      32    4921

Cuts at:   8178    8210    2219 
   Size:         32    2227

  Fragments arranged by size: 

                4921    2227     502     371      37      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    3257    1316 
   Size:       1941    6277
 
XbaI T'CTAG_A

Cuts at:   4004    5554    4004 
   Size:       1550    6668
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2099    2953    2980    4709    2099 
   Size:        854      27    1729    5608

  Fragments arranged by size: 

                5608    1729     854      27
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       8218
 
XmaI C'CCGG_G

Cuts at:   1870    2521    2756    3763    4780    4833    5543    1870 
   Size:        651     235    1007    1017      53     710    4545

  Fragments arranged by size: 

                4545    1017    1007     710     651     235      53
 
XmnI GAAnn'nnTTC

Cuts at:   6347    6347 
   Size:       8218
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    6026     276 
   Size:         53      83     186     936    4492    2468

  Fragments arranged by size: 

                4492    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PstI     PvuI    PvuII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AscI    AvrII    BfrBI    BsiWI    BstXI  BstZ17I    EcoRV
    FspAI     NruI     NsiI     PacI    PspXI     PsrI    RsrII     SwaI
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