Restriction map for
pFN21ASDA0221
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# NO CUT ENZYMES ( 85 enzymes) #
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AccIII AcsI AflII AgeI AhdI
AocI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbvCI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BseAI BsiEI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FspI HindIII
HpaI KspI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
PvuI RsrII SacI SacII ScaI
SfuI SnaBI SpeI SspBI SspI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 1533 GACGTC GACGT^C
Acc65I 1814 GGTACC G^GTACC
AclI 1317 AACGTT AA^CGTT
AflIII 1879 ACATGT A^C[GA][CT]GT
Alw44I 1232 GTGCAC G^TGCAC
ApaLI 1232 GTGCAC G^TGCAC
Asp718I 1814 GGTACC G^GTACC
BalI 1907 TGGCCA TGG^CCA
BbrPI 2956 CACGTG CAC^GTG
BclI 2936 TGATCA T^GATCA
BsaBI 2400 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1333 GAGACC GGTCTC(1/5)
BsaWI 2138 ACCGGA [AT]^CCGG[AT]
BsiWI 14 CGTACG C^GTACG
BspHI 2819 TCATGA T^CATGA
BspLU11I 1879 ACATGT A^CATGT
BssSI 1375 CACGAG CACGAG(-5/-1)
BstXI 2574 CCAAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI 2936 TGATCA T^GATCA
FseI 2890 GGCCGGCC GGCCGG^CC
KpnI 1814 GGTACC GGTAC^C
MamI 2400 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1907 TGGCCA TGG^CCA
MscI 1907 TGGCCA TGG^CCA
PmaCI 2956 CACGTG CAC^GTG
PmlI 2956 CACGTG CAC^GTG
Psp1406I 1317 AACGTT AA^CGTT
RcaI 2819 TCATGA T^CATGA
SalI 1281 GTCGAC G^TCGAC
SapI 2082 GCTCTTC GCTCTTC(1/4)
SbfI 2124 CCTGCAGG CCTGCA^GG
SfiI 148 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1890 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1742 GCATGC GCATG^C
Sse8387I 2124 CCTGCAGG CCTGCA^GG
TfiI 1022 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 1281 GTCGAC GT^[AC][GT]AC
1549 GTCTAC
Aor51HI 1415 AGCGCT AGC^GCT
2429 AGCGCT
BfaI 122 CTAG C^TAG
2951 CTAG
BstEII 501 GGTGACC G^GT[ACGT]ACC
1894 GGTGACC
BstPI 501 GGTGACC G^GT[ACGT]ACC
1894 GGTGACC
BstYI 731 AGATCC [GA]^GATC[CT]
1631 AGATCC
DraI 2589 TTTAAA TTT^AAA
3292 TTTAAA
DraIII 475 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1375 CACGAGGTG
Eco47III 1415 AGCGCT AGC^GCT
2429 AGCGCT
EcoO65I 501 GGTGACC G^GT[ACGT]ACC
1894 GGTGACC
HincII 1281 GTCGAC GT[CT]^[GA]AC
2791 GTCAAC
HindII 1281 GTCGAC GT[CT]^[GA]AC
2791 GTCAAC
MaeI 122 CTAG C^TAG
2951 CTAG
MflI 731 AGATCC [GA]^GATC[CT]
1631 AGATCC
NaeI 2891 GCCGGC GCC^GGC
2944 GCCGGC
NgoMIV 2891 GCCGGC G^CCGGC
2944 GCCGGC
SexAI 2438 ACCTGGT A^CC[AT]GGT
2990 ACCTGGT
SmlI 655 CTCAAG C^T[CT][GA]AG
974 CTCAAG
Tsp509I 1077 AATT ^AATT
1189 AATT
XhoII 731 AGATCC [GA]^GATC[CT]
1631 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI Asp700I AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI XmnI