(Circular) (Six-base) MAPSORT of: pFN21ASDA0221.seq Check: 3270 from: 1 to: 8116 pFN21ASDA0221 8116 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4725 4725 Size: 8116 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3560 5926 278 Size: 53 83 186 936 2024 2366 2468 Fragments arranged by size: 2468 2366 2024 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 3573 5459 1937 Size: 1368 268 1886 4594 Fragments arranged by size: 4594 1886 1368 268 Acc65I G'GTAC_C Cuts at: 3837 5437 3837 Size: 1600 6516 AclI AA'CG_TT Cuts at: 1563 3341 6243 6616 1563 Size: 1778 2902 373 3063 Fragments arranged by size: 3063 2902 1778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3002 4418 6179 7563 7831 1632 Size: 1370 1416 1761 1384 268 1917 Fragments arranged by size: 1917 1761 1416 1384 1370 268 AfeI AGC'GCT Cuts at: 3440 4454 3440 Size: 1014 7102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7919 AflIII A'CryG_T Cuts at: 1203 3902 7015 7992 1203 Size: 2699 3113 977 1327 Fragments arranged by size: 3113 2699 1327 977 AgeI A'CCGG_T Cuts at: 6947 6947 Size: 8116 AhdI GACnn_n'nnGTC Cuts at: 6845 6845 Size: 8116 AleI CACnn'nnGTG Cuts at: 1166 3905 1166 Size: 2739 5377 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2676 1184 Size: 1492 6624 AlwNI CAG_nnn'CTG Cuts at: 2397 2716 3784 3793 5859 7431 2397 Size: 319 1068 9 2066 1572 3082 Fragments arranged by size: 3082 2066 1572 1068 319 9 ApaI G_GGCC'C Cuts at: 2046 2262 2547 2946 3533 4058 2046 Size: 216 285 399 587 525 6104 Fragments arranged by size: 6104 587 525 399 285 216 ApaLI G'TGCA_C Cuts at: 3255 6173 7329 3255 Size: 2918 1156 4042 Fragments arranged by size: 4042 2918 1156 ApoI r'AATT_y Cuts at: 1493 5425 5651 5803 7912 1493 Size: 3932 226 152 2109 1697 Fragments arranged by size: 3932 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6670 160 Size: 872 5638 1606 Fragments arranged by size: 5638 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8116 AvaI C'yCGr_G Cuts at: 1870 1943 2182 2206 3922 4010 4333 4828 4866 Size: 73 239 24 1716 88 323 495 38 Cuts at: 4866 5441 1870 Size: 575 4545 Fragments arranged by size: 4545 1716 575 495 323 239 88 73 38 24 BamHI G'GATC_C Cuts at: 5446 5813 5446 Size: 367 7749 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2096 3837 3911 4337 4406 Size: 325 217 627 309 1741 74 426 69 Cuts at: 4406 5081 5210 5437 6892 618 Size: 675 129 227 1455 1842 Fragments arranged by size: 1842 1741 1455 675 627 426 325 309 227 217 129 74 69 BanII G_rGCy'C Cuts at: 1391 2046 2114 2228 2262 2547 2946 3533 4058 Size: 655 68 114 34 285 399 587 525 Cuts at: 4058 4214 4837 5435 1391 Size: 156 623 598 4072 Fragments arranged by size: 4072 655 623 598 587 525 399 285 156 114 68 34 BbeI G_GCGC'C Cuts at: 2100 3915 4410 5214 2100 Size: 1815 495 804 5002 Fragments arranged by size: 5002 1815 804 495 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2358 2587 3500 3554 928 Size: 971 459 229 913 54 5490 Fragments arranged by size: 5490 971 913 459 229 54 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6307 6341 1903 Size: 34 4370 34 3678 Fragments arranged by size: 4370 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6008 7218 6008 Size: 1210 6906 BclI T'GATC_A Cuts at: 1548 4959 1548 Size: 3411 4705 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2178 2211 3537 Size: 107 122 71 631 605 506 33 1326 Cuts at: 3537 4053 4871 5265 6727 136 Size: 516 818 394 1462 1525 Fragments arranged by size: 1525 1462 1326 818 631 605 516 506 394 122 107 71 33 BglII A'GATC_T Cuts at: 8111 8111 Size: 8116 BlpI GC'TnA_GC Cuts at: 5533 5533 Size: 8116 Bme1580I G_kGCm'C Cuts at: 1790 2046 2262 2547 2785 2946 3259 3533 4058 Size: 256 216 285 238 161 313 274 525 Cuts at: 4058 4340 6177 7333 1790 Size: 282 1837 1156 2573 Fragments arranged by size: 2573 1837 1156 525 313 285 282 274 256 238 216 161 BmgBI CAC'GTC Cuts at: 1318 2465 3193 5332 1318 Size: 1147 728 2139 4102 Fragments arranged by size: 4102 2139 1147 728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2701 3816 4052 Size: 416 53 83 399 24 1284 1115 236 Cuts at: 4052 5398 5847 6805 442 Size: 1346 449 958 1753 Fragments arranged by size: 1753 1346 1284 1115 958 449 416 399 236 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8116 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2585 2837 2873 2882 2978 4202 6776 1239 Size: 1346 252 36 9 96 1224 2574 2579 Fragments arranged by size: 2579 2574 1346 1224 252 96 36 9 Bpu10I CC'TnA_GC Cuts at: 3771 3771 Size: 8116 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2663 2982 6241 7106 7404 2663 Size: 319 3259 865 298 3375 Fragments arranged by size: 3375 3259 865 319 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3350 6779 882 Size: 617 1851 3429 2219 Fragments arranged by size: 3429 2219 1851 617 BsaAI yAC'GTr Cuts at: 493 1204 4303 4981 5355 5413 5907 493 Size: 711 3099 678 374 58 494 2702 Fragments arranged by size: 3099 2702 711 678 494 374 58 BsaBI GATnn'nnATC Cuts at: 4427 8110 4427 Size: 3683 4433 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 2159 2916 3557 Size: 53 83 186 936 564 62 757 641 Cuts at: 3557 3912 4407 4536 4627 4662 5113 5189 5211 Size: 355 495 129 91 35 451 76 22 Cuts at: 5211 5923 6305 6940 275 Size: 712 382 635 1451 Fragments arranged by size: 1451 936 757 712 641 635 564 495 451 382 355 186 129 91 83 76 62 53 35 22 BsaWI w'CCGG_w Cuts at: 4161 6549 6947 7221 7368 7779 4161 Size: 2388 398 274 147 411 4498 Fragments arranged by size: 4498 2388 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2810 2840 3803 3833 4196 Size: 30 165 30 1407 30 963 30 363 Cuts at: 4196 4226 1178 Size: 30 5068 Fragments arranged by size: 5068 1407 963 363 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2591 2834 2897 4334 1186 Size: 907 498 243 63 1437 4968 Fragments arranged by size: 4968 1437 907 498 243 63 BseYI C'CCAG_C Cuts at: 2045 4051 4126 5144 5274 7319 7815 2045 Size: 2006 75 1018 130 2045 496 2346 Fragments arranged by size: 2346 2045 2006 1018 496 130 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2516 2668 3722 3883 4158 4478 4487 Size: 573 780 152 1054 161 275 320 9 Cuts at: 4487 1163 Size: 4792 Fragments arranged by size: 4792 1054 780 573 320 275 161 152 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5566 6327 7355 1540 Size: 275 186 3565 761 1028 2301 Fragments arranged by size: 3565 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3259 3619 4596 6177 6262 7333 3259 Size: 360 977 1581 85 1071 4042 Fragments arranged by size: 4042 1581 1071 977 360 85 BsiWI C'GTAC_G Cuts at: 2037 2037 Size: 8116 BsmI GAATG_Cn' Cuts at: 5627 5720 5627 Size: 93 8023 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2114 2228 2262 2547 2785 2946 Size: 399 256 68 114 34 285 238 161 Cuts at: 2946 3259 3533 3619 4058 4214 4340 4596 4837 Size: 313 274 86 439 156 126 256 241 Cuts at: 4837 5435 6177 6262 7333 1391 Size: 598 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 598 439 399 313 285 274 256 256 241 238 161 156 126 114 86 85 68 34 BspEI T'CCGG_A Cuts at: 7779 7779 Size: 8116 BspHI T'CATG_A Cuts at: 4842 6005 7735 4842 Size: 1163 1730 5223 Fragments arranged by size: 5223 1730 1163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2242 4141 4725 4779 5471 844 Size: 1398 1899 584 54 692 3489 Fragments arranged by size: 3489 1899 1398 692 584 54 BsrBI CCG'CTC Cuts at: 1394 1823 3948 4705 4762 5368 6003 1394 Size: 429 2125 757 57 606 635 3507 Fragments arranged by size: 3507 2125 757 635 606 429 57 BsrDI GCAATG_nn' Cuts at: 65 1248 6611 6785 65 Size: 1183 5363 174 1396 Fragments arranged by size: 5363 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3601 3914 4914 4967 6760 6947 1700 Size: 110 1791 313 1000 53 1793 187 2869 Fragments arranged by size: 2869 1793 1791 1000 313 187 110 53 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8116 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8116 BssSI C'ACGA_G Cuts at: 3398 6176 7188 3398 Size: 2778 1012 4326 Fragments arranged by size: 4326 2778 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3784 4474 4744 794 Size: 2990 690 270 4166 Fragments arranged by size: 4166 2990 690 270 BstBI TT'CG_AA Cuts at: 2007 6920 2007 Size: 4913 3203 BstEII G'GTnAC_C Cuts at: 1184 2524 3917 1184 Size: 1340 1393 5383 Fragments arranged by size: 5383 1393 1340 BstXI CCAn_nnnn'nTGG Cuts at: 2029 4604 2029 Size: 2575 5541 BstYI r'GATC_y Cuts at: 1972 2754 3654 5446 5813 6198 6215 7667 7753 Size: 782 900 1792 367 385 17 1452 86 Cuts at: 7753 7765 8111 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1792 1452 900 782 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3348 3633 4002 4802 513 Size: 552 2283 285 369 800 3827 Fragments arranged by size: 3827 2283 800 552 369 285 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3234 3613 487 Size: 683 56 2008 379 4990 Fragments arranged by size: 4990 2008 683 379 56 BtsI GCAGTG_nn' Cuts at: 2711 4042 4230 5632 6426 6446 2711 Size: 1331 188 1402 794 20 4381 Fragments arranged by size: 4381 1402 1331 794 188 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8116 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2446 2481 529 Size: 35 1882 35 6164 Fragments arranged by size: 6164 1882 35 35 DraI TTT'AAA Cuts at: 4614 5317 5420 4614 Size: 703 103 7310 Fragments arranged by size: 7310 703 103 DraIII CAC_nnn'GTG Cuts at: 2503 3403 2503 Size: 900 7216 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3364 3556 5398 7123 809 Size: 507 2048 192 1842 1725 1802 Fragments arranged by size: 2048 1842 1802 1725 507 192 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2616 3187 3561 Size: 54 1425 50 275 18 786 571 374 Cuts at: 3561 3930 4080 5563 6452 8081 8 Size: 369 150 1483 889 1629 43 Fragments arranged by size: 1629 1483 1425 889 786 571 374 369 275 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5563 1537 Size: 275 3751 4090 Fragments arranged by size: 4090 3751 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 3035 4112 6046 1353 Size: 792 890 1077 1934 3423 Fragments arranged by size: 3423 1934 1077 890 792 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4133 6703 7077 7223 387 Size: 1064 138 2544 2570 374 146 1280 Fragments arranged by size: 2570 2544 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2585 2837 2873 2882 2978 3002 4202 Size: 393 953 252 36 9 96 24 1200 Cuts at: 4202 4418 6179 6776 7563 7831 1239 Size: 216 1761 597 787 268 1524 Fragments arranged by size: 1761 1524 1200 953 787 597 393 268 252 216 96 36 24 9 EcoNI CCTnn'n_nnAGG Cuts at: 2534 3316 3524 5070 5219 2534 Size: 782 208 1546 149 5431 Fragments arranged by size: 5431 1546 782 208 149 EcoO109I rG'GnC_Cy Cuts at: 2371 2544 2857 2943 3022 3160 3202 3454 3529 Size: 173 313 86 79 138 42 252 75 Cuts at: 3529 2371 Size: 6958 Fragments arranged by size: 6958 313 252 173 138 86 79 75 42 EcoRI G'AATT_C Cuts at: 5425 5803 5425 Size: 378 7738 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3179 5143 3179 Size: 1964 6152 FseI GG_CCGG'CC Cuts at: 4918 4918 Size: 8116 FspI TGC'GCA Cuts at: 6622 6622 Size: 8116 HaeII r_GCGC'y Cuts at: 1678 2100 3442 3915 4117 4185 4410 4456 5214 Size: 422 1342 473 202 68 225 46 758 Cuts at: 5214 5339 7263 1678 Size: 125 1924 2531 Fragments arranged by size: 2531 1924 1342 758 473 422 225 202 125 68 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3344 3376 3803 3835 5174 Size: 32 135 32 1772 32 427 32 1339 Cuts at: 5174 5206 5303 5335 6757 6789 6831 6863 1373 Size: 32 97 32 1422 32 42 32 2626 Fragments arranged by size: 2626 1772 1422 1339 427 135 97 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 4816 5460 5706 669 Size: 988 281 1368 1510 644 246 3079 Fragments arranged by size: 3079 1510 1368 988 644 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8116 HpaI GTT'AAC Cuts at: 5706 5706 Size: 8116 KasI G'GCGC_C Cuts at: 2096 3911 4406 5210 2096 Size: 1815 495 804 5002 Fragments arranged by size: 5002 1815 804 495 KpnI G_GTAC'C Cuts at: 3841 5441 3841 Size: 1600 6516 MfeI C'AATT_G Cuts at: 5715 5715 Size: 8116 MluI A'CGCG_T Cuts at: 7992 7992 Size: 8116 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4218 7230 7414 1305 Size: 2913 3012 184 2007 Fragments arranged by size: 3012 2913 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3932 10 Size: 54 1425 343 2100 4194 Fragments arranged by size: 4194 2100 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 3632 3905 4670 4853 5234 6074 Size: 648 861 1605 273 765 183 381 840 Cuts at: 6074 6433 6592 518 Size: 359 159 2042 Fragments arranged by size: 2042 1605 861 840 765 648 381 359 273 183 159 MspA1I CmG'CkG Cuts at: 1606 2716 2730 2776 3430 3793 4024 4051 4238 Size: 1110 14 46 654 363 231 27 187 Cuts at: 4238 4485 5532 6209 7357 7602 1606 Size: 247 1047 677 1148 245 2120 Fragments arranged by size: 2120 1148 1110 1047 677 654 363 247 245 231 187 46 27 14 NaeI GCC'GGC Cuts at: 4916 4969 4916 Size: 53 8063 NarI GG'CG_CC Cuts at: 2097 3912 4407 5211 2097 Size: 1815 495 804 5002 Fragments arranged by size: 5002 1815 804 495 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7564 NdeI CA'TA_TG Cuts at: 387 387 Size: 8116 NgoMIV G'CCGG_C Cuts at: 4914 4967 4914 Size: 53 8063 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8116 NotI GC'GGCC_GC Cuts at: 5563 5563 Size: 8116 NspI r_CATG'y Cuts at: 1132 1229 2897 3118 3769 3906 4477 4930 5474 Size: 97 1668 221 651 137 571 453 544 Cuts at: 5474 7019 1132 Size: 1545 2229 Fragments arranged by size: 2229 1668 1545 651 571 544 453 221 137 97 PasI CC'CwG_GG Cuts at: 1348 2548 4309 1348 Size: 1200 1761 5155 Fragments arranged by size: 5155 1761 1200 PciI A'CATG_T Cuts at: 3902 7015 3902 Size: 3113 5003 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 3403 4516 5039 5185 1108 Size: 1212 1083 1113 523 146 4039 Fragments arranged by size: 4039 1212 1113 1083 523 146 PfoI T'CCnGG_A Cuts at: 2067 2562 2721 5137 2067 Size: 495 159 2416 5046 Fragments arranged by size: 5046 2416 495 159 PmeI GTTT'AAAC Cuts at: 5420 5420 Size: 8116 PmlI CAC'GTG Cuts at: 4981 4981 Size: 8116 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2140 6185 6217 7698 7730 2108 Size: 32 4045 32 1481 32 2494 Fragments arranged by size: 4045 2494 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2371 2857 3022 3160 3202 2371 Size: 486 165 138 42 7285 Fragments arranged by size: 7285 486 165 138 42 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8116 PsiI TTA'TAA Cuts at: 5686 5686 Size: 8116 PspOMI G'GGCC_C Cuts at: 2042 2258 2543 2942 3529 4054 2042 Size: 216 285 399 587 525 6104 Fragments arranged by size: 6104 587 525 399 285 216 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4463 4495 4463 Size: 32 8084 PstI C_TGCA'G Cuts at: 830 2277 2393 3780 3789 4152 4237 5074 5468 Size: 1447 116 1387 9 363 85 837 394 Cuts at: 5468 830 Size: 3478 Fragments arranged by size: 3478 1447 1387 837 394 363 116 85 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8116 PvuII CAG'CTG Cuts at: 2776 3430 3793 4024 4051 2776 Size: 654 363 231 27 6841 Fragments arranged by size: 6841 654 363 231 27 SalI G'TCGA_C Cuts at: 1936 3304 5458 1936 Size: 1368 2154 4594 Fragments arranged by size: 4594 2154 1368 SapI GCTCTTCn'nnn_ Cuts at: 4112 4112 Size: 8116 SbfI CC_TGCA'GG Cuts at: 4152 5468 4152 Size: 1316 6800 ScaI AGT'ACT Cuts at: 6364 8076 6364 Size: 1712 6404 SexAI A'CCwGG_T Cuts at: 4461 5013 4461 Size: 552 7564 SfcI C'TryA_G Cuts at: 826 1045 2273 2389 3776 3785 4148 4233 5070 Size: 219 1228 116 1387 9 363 85 837 Cuts at: 5070 5464 6599 7280 7471 826 Size: 394 1135 681 191 1471 Fragments arranged by size: 1471 1387 1228 1135 837 681 394 363 219 191 116 85 9 SfiI GGCCn_nnn'nGGCC Cuts at: 2178 2178 Size: 8116 SfoI GGC'GCC Cuts at: 2098 3913 4408 5212 2098 Size: 1815 495 804 5002 Fragments arranged by size: 5002 1815 804 495 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8116 SgrAI Cr'CCGG_yG Cuts at: 1700 3914 1700 Size: 2214 5902 SmaI CCC'GGG Cuts at: 1872 2184 2208 4830 4868 5443 1872 Size: 312 24 2622 38 575 4545 Fragments arranged by size: 4545 2622 575 312 38 24 SmlI C'TyrA_G Cuts at: 820 1017 1943 2678 2997 6220 7121 7383 820 Size: 197 926 735 319 3223 901 262 1553 Fragments arranged by size: 3223 1553 926 901 735 319 262 197 SnaBI TAC'GTA Cuts at: 493 5413 5907 493 Size: 4920 494 2702 Fragments arranged by size: 4920 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8116 SphI G_CATG'C Cuts at: 1132 3769 5474 1132 Size: 2637 1705 3774 Fragments arranged by size: 3774 2637 1705 SrfI GCCC'GGGC Cuts at: 2184 2208 2184 Size: 24 8092 SspI AAT'ATT Cuts at: 5 52 6040 5 Size: 47 5988 2081 Fragments arranged by size: 5988 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8116 StyI C'CwwG_G Cuts at: 513 1065 4083 4597 5302 5555 513 Size: 552 3018 514 705 253 3074 Fragments arranged by size: 3074 3018 705 552 514 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3242 3986 4373 5041 6143 6160 6313 Size: 1255 1662 744 387 668 1102 17 153 Cuts at: 6313 6498 6943 325 Size: 185 445 1498 Fragments arranged by size: 1662 1498 1255 1102 744 668 445 387 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4470 4929 6362 Size: 274 80 33 51 1443 2493 459 1433 Cuts at: 6362 8074 96 Size: 1712 138 Fragments arranged by size: 2493 1712 1443 1433 459 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2810 2842 3281 3313 8076 8108 2810 Size: 32 439 32 4763 32 2818 Fragments arranged by size: 4763 2818 439 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8116 XbaI T'CTAG_A Cuts at: 5452 5452 Size: 8116 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2317 4949 5182 2317 Size: 2632 233 5251 Fragments arranged by size: 5251 2632 233 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8116 XmaI C'CCGG_G Cuts at: 1870 2182 2206 4828 4866 5441 1870 Size: 312 24 2622 38 575 4545 Fragments arranged by size: 4545 2622 575 312 38 24 XmnI GAAnn'nnTTC Cuts at: 2450 2473 2936 4559 6245 2450 Size: 23 463 1623 1686 4321 Fragments arranged by size: 4321 1686 1623 463 23 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3558 5924 276 Size: 53 83 186 936 2024 2366 2468 Fragments arranged by size: 2468 2366 2024 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FspAI NruI NsiI PacI PspXI RsrII SacI SacII SanDI SwaI