Restriction map for
pFN21ASDA0212
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AlwI AocI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BglII
BlnI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRV FspI HindIII HpaI
KpnI MfeI MflI MluI MunI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 950 GTCGAC GT^[AC][GT]AC
AccIII 1016 TCCGGA T^CCGGA
AflII 1206 CTTAAG C^TTAAG
Alw44I 1834 GTGCAC G^TGCAC
ApaLI 1834 GTGCAC G^TGCAC
BalI 983 TGGCCA TGG^CCA
BbeI 358 GGCGCC GGCGC^C
BclI 626 TGATCA T^GATCA
BfrI 1206 CTTAAG C^TTAAG
BlpI 114 GCTCAGC GC^T[ACGT]AGC
BpmI 1872 CTCCAG CTGGAG(16/14)
Bpu1102I 114 GCTCAGC GC^T[ACGT]AGC
BsaAI 304 TACGTG [CT]AC^GT[GA]
BsaBI 1759 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1016 TCCGGA T^CCGGA
BspEI 1016 TCCGGA T^CCGGA
BspHI 1851 TCATGA T^CATGA
BspMI 1335 GCAGGT ACCTGC(4/8)
BsrBI 112 CCGCTC CCGCTC(-3/-3)
CelII 114 GCTCAGC GC^T[ACGT]AGC
DpnII 627 GATC ^GATC
EcoNI 1500 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1789 GAATTC G^AATTC
EcoT22I 563 ATGCAT ATGCA^T
FbaI 626 TGATCA T^GATCA
FseI 181 GGCCGGCC GGCCGG^CC
KasI 358 GGCGCC G^GCGCC
MaeIII 746 GTGAC ^GT[ACGT]AC
MamI 1759 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 627 GATC ^GATC
MluNI 983 TGGCCA TGG^CCA
MroI 1016 TCCGGA T^CCGGA
MscI 983 TGGCCA TGG^CCA
NaeI 182 GCCGGC GCC^GGC
NarI 358 GGCGCC GG^CGCC
NdeI 1392 CATATG CA^TATG
NdeII 627 GATC ^GATC
NgoMIV 182 GCCGGC G^CCGGC
NsiI 563 ATGCAT ATGCA^T
PflMI 792 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1851 TCATGA T^CATGA
SacI 460 GAGCTC GAGCT^C
SalI 950 GTCGAC G^TCGAC
Sau3AI 627 GATC ^GATC
SfoI 358 GGCGCC GGC^GCC
SmlI 1206 CTTAAG C^T[CT][GA]AG
SspI 1667 AATATT AAT^ATT
StuI 1316 AGGCCT AGG^CCT
TaqI 951 TCGA T^CGA
Tsp45I 746 GTGAC ^GT[GC]AC
TthHB8I 951 TCGA T^CGA
Van91I 792 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 263 GGCGTC G[GA]^CG[CT]C
358 GGCGCC
AflIII 1271 ACATGT A^C[GA][CT]GT
1724 ACATGT
AvaI 168 CTCGGG C^[CT]CG[GA]G
854 CTCGGG
BciVI 570 GGATAC GTATCC(6/5)
196 GTATCC
BglI 143 GCCCCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
204 GCCGTCGTGGC
BsaHI 263 GGCGTC G[GA]^CG[CT]C
358 GGCGCC
BsaWI 1016 TCCGGA [AT]^CCGG[AT]
1631 ACCGGA
BsoBI 168 CTCGGG C^[CT]CG[GA]G
854 CTCGGG
BspLU11I 1271 ACATGT A^CATGT
1724 ACATGT
BsrFI 182 GCCGGC [GA]^CCGG[CT]
916 GCCGGT
Cfr10I 182 GCCGGC [GA]^CCGG[CT]
916 GCCGGT
EagI 315 CGGCCG C^GGCCG
483 CGGCCG
EclXI 315 CGGCCG C^GGCCG
483 CGGCCG
Eco52I 315 CGGCCG C^GGCCG
483 CGGCCG
Hin1I 263 GGCGTC G[GA]^CG[CT]C
358 GGCGCC
HincII 950 GTCGAC GT[CT]^[GA]AC
1261 GTCAAC
HindII 950 GTCGAC GT[CT]^[GA]AC
1261 GTCAAC
HphI 745 GGTGA GGTGA(8/7)
858 GGTGA
MslI 971 CATTTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1932 CATCTACATG
NcoI 51 CCATGG C^CATGG
1780 CCATGG
PpuMI 503 GGGACCC [GA]G^G[AT]CC[CT]
686 GGGTCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI Aor51HI ApaI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EarI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI Ksp632I KspI MaeI MaeII
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacII Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp509I TspRI XmnI