Restriction map for pFN21ASDA0212
 (Linear) (Six-base) MAPSORT of: KIAA0212.seq  Check: 7985  from: 1  to: 1971



KIAA0212.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1326    1971 
   Size:       1326     645
 
AccI GT'mk_AC

Cuts at:      0     951    1971 
   Size:        951    1020
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1820    1971 
   Size:       1820     151
 
AfeI AGC'GCT

Cuts at:      0      14     106     349    1971 
   Size:         14      92     243    1622

  Fragments arranged by size: 

                1622     243      92      14
 
AflII C'TTAA_G

Cuts at:      0    1206    1971 
   Size:       1206     765
 
AflIII A'CryG_T

Cuts at:      0    1271    1724    1971 
   Size:       1271     453     247

  Fragments arranged by size: 

                1271     453     247
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     395    1954    1971 
   Size:        395    1559      17

  Fragments arranged by size: 

                1559     395      17
 
AlwNI CAG_nnn'CTG

Cuts at:      0     135    1341    1560    1971 
   Size:        135    1206     219     411

  Fragments arranged by size: 

                1206     411     219     135
 
ApaI G_GGCC'C

Cuts at:      0      86     176     494    1971 
   Size:         86      90     318    1477

  Fragments arranged by size: 

                1477     318      90      86
 
ApaLI G'TGCA_C

Cuts at:      0    1834    1971 
   Size:       1834     137
 
ApoI r'AATT_y

Cuts at:      0     591     934    1789    1971 
   Size:        591     343     855     182

  Fragments arranged by size: 

                 855     591     343     182
 
AvaI C'yCGr_G

Cuts at:      0     168     854    1971 
   Size:        168     686    1117

  Fragments arranged by size: 

                1117     686     168
 
BanI G'GyrC_C

Cuts at:      0     358    1096    1408    1971 
   Size:        358     738     312     563

  Fragments arranged by size: 

                 738     563     358     312
 
BanII G_rGCy'C

Cuts at:      0      86     145     176     247     464     494    1971 
   Size:         86      59      31      71     217      30    1477

  Fragments arranged by size: 

                1477     217      86      71      59      31      30
 
BbeI G_GCGC'C

Cuts at:      0     362    1971 
   Size:        362    1609
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      63    1166    1634    1697    1971 
   Size:         63    1103     468      63     274

  Fragments arranged by size: 

                1103     468     274      63      63
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     207     564    1971 
   Size:        207     357    1407

  Fragments arranged by size: 

                1407     357     207
 
BclI T'GATC_A

Cuts at:      0     626    1971 
   Size:        626    1345
 
BfrBI ATG'CAT

Cuts at:      0     565    1971 
   Size:        565    1406
 
BglI GCCn_nnn'nGGC

Cuts at:      0     149     210    1971 
   Size:        149      61    1761

  Fragments arranged by size: 

                1761     149      61
 
BlpI GC'TnA_GC

Cuts at:      0     115    1971 
   Size:        115    1856
 
Bme1580I G_kGCm'C

Cuts at:      0      86     176     384     494    1413    1838    1971 
   Size:         86      90     208     110     919     425     133

  Fragments arranged by size: 

                 919     425     208     133     110      90      86
 
BmgBI CAC'GTC

Cuts at:      0    1608    1971 
   Size:       1608     363
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     306    1080    1971 
   Size:        306     774     891

  Fragments arranged by size: 

                 891     774     306
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1857    1971 
   Size:       1857     114
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1238    1464    1651    1971 
   Size:       1238     226     187     320

  Fragments arranged by size: 

                1238     320     226     187
 
BsaAI yAC'GTr

Cuts at:      0     306    1971 
   Size:        306    1665
 
BsaBI GATnn'nnATC

Cuts at:      0    1763    1971 
   Size:       1763     208
 
BsaHI Gr'CG_yC

Cuts at:      0     264     359    1971 
   Size:        264      95    1612

  Fragments arranged by size: 

                1612     264      95
 
BsaWI w'CCGG_w

Cuts at:      0    1016    1631    1971 
   Size:       1016     615     340

  Fragments arranged by size: 

                1016     615     340
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1861    1891    1971 
   Size:       1861      30      80

  Fragments arranged by size: 

                1861      80      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     153     802    1489    1920    1971 
   Size:         25     128     649     687     431      51

  Fragments arranged by size: 

                 687     649     431     128      51      25
 
BseYI C'CCAG_C

Cuts at:      0      78      85     288     309    1971 
   Size:         78       7     203      21    1662

  Fragments arranged by size: 

                1662     203      78      21       7
 
BsiEI CG_ry'CG

Cuts at:      0     318     486     498    1971 
   Size:        318     168      12    1473

  Fragments arranged by size: 

                1473     318     168      12
 
BsiHKAI G_wGCw'C

Cuts at:      0      35     464    1838    1971 
   Size:         35     429    1374     133

  Fragments arranged by size: 

                1374     429     133      35
 
Bsp1286I G_dGCh'C

Cuts at:      0      35      86     145     176     247     384     464     494 
   Size:         35      51      59      31      71     137      80      30

Cuts at:    494    1413    1838    1971 
   Size:        919     425     133

  Fragments arranged by size: 

                 919     425     137     133      80      71      59      51
                  35      31      30
 
BspEI T'CCGG_A

Cuts at:      0    1016    1971 
   Size:       1016     955
 
BspHI T'CATG_A

Cuts at:      0    1851    1971 
   Size:       1851     120
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1326    1971 
   Size:       1326     645
 
BsrBI CCG'CTC

Cuts at:      0     114    1971 
   Size:        114    1857
 
BsrDI GCAATG_nn'

Cuts at:      0      10    1029    1050    1236    1971 
   Size:         10    1019      21     186     735

  Fragments arranged by size: 

                1019     735     186      21      10
 
BsrFI r'CCGG_y

Cuts at:      0     182     916    1971 
   Size:        182     734    1055

  Fragments arranged by size: 

                1055     734     182
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     296    1075    1960    1971 
   Size:        296     779     885      11

  Fragments arranged by size: 

                 885     779     296      11
 
BtgI C'CryG_G

Cuts at:      0      51     176     259     324     480     486     498    1780 
   Size:         51     125      83      65     156       6      12    1282

Cuts at:   1780    1971 
   Size:        191

  Fragments arranged by size: 

                1282     191     156     125      83      65      51      12
                   6
 
BtsI GCAGTG_nn'

Cuts at:      0     474    1001    1971 
   Size:        474     527     970

  Fragments arranged by size: 

                 970     527     474
 
EaeI y'GGCC_r

Cuts at:      0     315     483     983    1971 
   Size:        315     168     500     988

  Fragments arranged by size: 

                 988     500     315     168
 
EagI C'GGCC_G

Cuts at:      0     315     483    1971 
   Size:        315     168    1488

  Fragments arranged by size: 

                1488     315     168
 
EarI CTCTTCn'nnn_

Cuts at:      0    1206    1462    1796    1971 
   Size:       1206     256     334     175

  Fragments arranged by size: 

                1206     334     256     175
 
EcoICRI GAG'CTC

Cuts at:      0     462    1971 
   Size:        462    1509
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1820    1857    1971 
   Size:       1820      37     114

  Fragments arranged by size: 

                1820     114      37
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1504    1971 
   Size:       1504     467
 
EcoO109I rG'GnC_Cy

Cuts at:      0      82     172     504     687    1443    1971 
   Size:         82      90     332     183     756     528

  Fragments arranged by size: 

                 756     528     332     183      90      82
 
EcoRI G'AATT_C

Cuts at:      0    1789    1971 
   Size:       1789     182
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     756    1539    1809    1971 
   Size:        756     783     270     162

  Fragments arranged by size: 

                 783     756     270     162
 
FseI GG_CCGG'CC

Cuts at:      0     186    1971 
   Size:        186    1785
 
HaeII r_GCGC'y

Cuts at:      0      16     108     351     362    1971 
   Size:         16      92     243      11    1609

  Fragments arranged by size: 

                1609     243      92      16      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       2      34     834     866     933     965    1293    1325 
   Size:          2      32     800      32      67      32     328      32

Cuts at:   1325    1971 
   Size:        646

  Fragments arranged by size: 

                 800     646     328      67      32      32      32      32
                   2
 
HincII GTy'rAC

Cuts at:      0     952    1263    1971 
   Size:        952     311     708

  Fragments arranged by size: 

                 952     708     311
 
KasI G'GCGC_C

Cuts at:      0     358    1971 
   Size:        358    1613
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1056    1904    1971 
   Size:       1056     848      67

  Fragments arranged by size: 

                1056     848      67
 
MscI TGG'CCA

Cuts at:      0     985    1971 
   Size:        985     986
 
MslI CAynn'nnrTG

Cuts at:      0     975    1936    1971 
   Size:        975     961      35

  Fragments arranged by size: 

                 975     961      35
 
MspA1I CmG'CkG

Cuts at:      0     261     326     356     375     482     500     910    1287 
   Size:        261      65      30      19     107      18     410     377

Cuts at:   1287    1437    1971 
   Size:        150     534

  Fragments arranged by size: 

                 534     410     377     261     150     107      65      30
                  19      18
 
NaeI GCC'GGC

Cuts at:      0     184    1971 
   Size:        184    1787
 
NarI GG'CG_CC

Cuts at:      0     359    1971 
   Size:        359    1612
 
NcoI C'CATG_G

Cuts at:      0      51    1780    1971 
   Size:         51    1729     191

  Fragments arranged by size: 

                1729     191      51
 
NdeI CA'TA_TG

Cuts at:      0    1393    1971 
   Size:       1393     578
 
NgoMIV G'CCGG_C

Cuts at:      0     182    1971 
   Size:        182    1789
 
NsiI A_TGCA'T

Cuts at:      0     567    1971 
   Size:        567    1404
 
NspI r_CATG'y

Cuts at:      0     411     905    1275    1728    1941    1971 
   Size:        411     494     370     453     213      30

  Fragments arranged by size: 

                 494     453     411     370     213      30
 
PciI A'CATG_T

Cuts at:      0    1271    1724    1971 
   Size:       1271     453     247

  Fragments arranged by size: 

                1271     453     247
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     798    1971 
   Size:        798    1173
 
PpuMI rG'GwC_Cy

Cuts at:      0     504     687    1971 
   Size:        504     183    1284

  Fragments arranged by size: 

                1284     504     183
 
PspOMI G'GGCC_C

Cuts at:      0      82     172     490    1971 
   Size:         82      90     318    1481

  Fragments arranged by size: 

                1481     318      90      82
 
PstI C_TGCA'G

Cuts at:      0     218    1316    1337    1442    1556    1971 
   Size:        218    1098      21     105     114     415

  Fragments arranged by size: 

                1098     415     218     114     105      21
 
PvuII CAG'CTG

Cuts at:      0     375    1287    1437    1971 
   Size:        375     912     150     534

  Fragments arranged by size: 

                 912     534     375     150
 
SacI G_AGCT'C

Cuts at:      0     464    1971 
   Size:        464    1507
 
SacII CC_GC'GG

Cuts at:      0     262     327     483     501    1971 
   Size:        262      65     156      18    1470

  Fragments arranged by size: 

                1470     262     156      65      18
 
SalI G'TCGA_C

Cuts at:      0     950    1971 
   Size:        950    1021
 
SanDI GG'GwC_CC

Cuts at:      0     504    1971 
   Size:        504    1467
 
SfcI C'TryA_G

Cuts at:      0     214     351    1312    1333    1438    1552    1971 
   Size:        214     137     961      21     105     114     419

  Fragments arranged by size: 

                 961     419     214     137     114     105      21
 
SfoI GGC'GCC

Cuts at:      0     360    1971 
   Size:        360    1611
 
SmlI C'TyrA_G

Cuts at:      0    1206    1971 
   Size:       1206     765
 
SspI AAT'ATT

Cuts at:      0    1669    1971 
   Size:       1669     302
 
StuI AGG'CCT

Cuts at:      0    1318    1971 
   Size:       1318     653
 
StyI C'CwwG_G

Cuts at:      0      51    1780    1818    1971 
   Size:         51    1729      38     153

  Fragments arranged by size: 

                1729     153      51      38
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     969    1084    1971 
   Size:        969     115     887

  Fragments arranged by size: 

                 969     887     115
 
TatI w'GTAC_w

Cuts at:      0     531    1568    1701    1971 
   Size:        531    1037     133     270

  Fragments arranged by size: 

                1037     531     270     133
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1322    1354    1817    1849    1971 
   Size:       1322      32     463      32     122

  Fragments arranged by size: 

                1322     463     122      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1273    1522    1789    1971 
   Size:       1273     249     267     182

  Fragments arranged by size: 

                1273     267     249     182
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI    AflII   AflIII     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BstXI     BtgI     BtsI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI     PciI    PflMI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII     SalI    SanDI
     SfcI     SfoI     SmlI     SspI     StuI     StyI    TaqII     TatI
     TstI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AgeI     AhdI     AleI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BglII
     BmtI     BplI   Bpu10I    BpuEI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EciI
    EcoRV     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NheI     NotI     NruI     PacI     PasI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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