Restriction map for
pFN21ASDA0167
(Linear) (Six-base) MAPSORT of: KIAA0167.seq Check: 9062 from: 1 to: 2508
KIAA0167.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1385 2508
Size: 1385 1123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 1402 1544 1565 2508
Size: 411 991 142 21 943
Fragments arranged by size:
991 943 411 142 21
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 751 2508
Size: 751 1757
AlwNI CAG_nnn'CTG
Cuts at: 0 291 412 717 883 2055 2508
Size: 291 121 305 166 1172 453
Fragments arranged by size:
1172 453 305 291 166 121
ApaI G_GGCC'C
Cuts at: 0 1785 2508
Size: 1785 723
ApoI r'AATT_y
Cuts at: 0 1395 1758 2508
Size: 1395 363 750
Fragments arranged by size:
1395 750 363
AvaI C'yCGr_G
Cuts at: 0 563 1197 1212 1403 2376 2508
Size: 563 634 15 191 973 132
Fragments arranged by size:
973 634 563 191 132 15
BanI G'GyrC_C
Cuts at: 0 378 1816 2064 2508
Size: 378 1438 248 444
Fragments arranged by size:
1438 444 378 248
BanII G_rGCy'C
Cuts at: 0 594 650 860 1259 1410 1447 1785 2234
Size: 594 56 210 399 151 37 338 449
Cuts at: 2234 2508
Size: 274
Fragments arranged by size:
594 449 399 338 274 210 151 56
37
BbeI G_GCGC'C
Cuts at: 0 1820 2068 2508
Size: 1820 248 440
Fragments arranged by size:
1820 440 248
BbsI GAAGACnn'nnnn_
Cuts at: 0 304 417 657 2508
Size: 304 113 240 1851
Fragments arranged by size:
1851 304 240 113
BbvCI CC'TCA_GC
Cuts at: 0 1239 1411 2508
Size: 1239 172 1097
Fragments arranged by size:
1239 1097 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 856 890 2508
Size: 856 34 1618
Fragments arranged by size:
1618 856 34
BclI T'GATC_A
Cuts at: 0 167 2508
Size: 167 2341
BfrBI ATG'CAT
Cuts at: 0 3 2508
Size: 3 2505
BglI GCCn_nnn'nGGC
Cuts at: 0 70 1735 2119 2329 2508
Size: 70 1665 384 210 179
Fragments arranged by size:
1665 384 210 179 70
BlpI GC'TnA_GC
Cuts at: 0 2071 2508
Size: 2071 437
Bme1580I G_kGCm'C
Cuts at: 0 381 1785 1864 2508
Size: 381 1404 79 644
Fragments arranged by size:
1404 644 381 79
BmgBI CAC'GTC
Cuts at: 0 1592 2130 2241 2280 2508
Size: 1592 538 111 39 228
Fragments arranged by size:
1592 538 228 111 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 1121 1331 2508
Size: 1121 210 1177
Fragments arranged by size:
1177 1121 210
BmtI G_CTAG'C
Cuts at: 0 2474 2508
Size: 2474 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 313 454 1454 2347 2508
Size: 106 207 141 1000 893 161
Fragments arranged by size:
1000 893 207 161 141 106
Bpu10I CC'TnA_GC
Cuts at: 0 1239 1411 1441 2508
Size: 1239 172 30 1067
Fragments arranged by size:
1239 1067 172 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 1430 2508
Size: 1430 1078
BsaAI yAC'GTr
Cuts at: 0 1796 2508
Size: 1796 712
BsaHI Gr'CG_yC
Cuts at: 0 1817 2065 2294 2489 2508
Size: 1817 248 229 195 19
Fragments arranged by size:
1817 248 229 195 19
BsaWI w'CCGG_w
Cuts at: 0 771 1391 1742 2508
Size: 771 620 351 766
Fragments arranged by size:
771 766 620 351
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2008 2038 2508
Size: 2008 30 470
Fragments arranged by size:
2008 470 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 521 1506 1633 2029 2098 2508
Size: 521 985 127 396 69 410
Fragments arranged by size:
985 521 410 396 127 69
BseYI C'CCAG_C
Cuts at: 0 225 373 401 739 1330 1534 2091 2407
Size: 225 148 28 338 591 204 557 316
Cuts at: 2407 2425 2443 2508
Size: 18 18 65
Fragments arranged by size:
591 557 338 316 225 204 148 65
28 18 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 2137 2508
Size: 892 1245 371
Fragments arranged by size:
1245 892 371
BsiEI CG_ry'CG
Cuts at: 0 2228 2508
Size: 2228 280
BsiHKAI G_wGCw'C
Cuts at: 0 594 860 895 1276 2077 2234 2508
Size: 594 266 35 381 801 157 274
Fragments arranged by size:
801 594 381 274 266 157 35
BsmI GAATG_Cn'
Cuts at: 0 152 2508
Size: 152 2356
BsmBI CGTCTCn'nnnn_
Cuts at: 0 472 2508
Size: 472 2036
Bsp1286I G_dGCh'C
Cuts at: 0 381 594 650 860 895 1259 1276 1410
Size: 381 213 56 210 35 364 17 134
Cuts at: 1410 1447 1785 1864 2077 2234 2508
Size: 37 338 79 213 157 274
Fragments arranged by size:
381 364 338 274 213 213 210 157
134 79 56 37 35 17
BspEI T'CCGG_A
Cuts at: 0 1742 2508
Size: 1742 766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1385 2508
Size: 1385 1123
BsrBI CCG'CTC
Cuts at: 0 197 1623 2175 2508
Size: 197 1426 552 333
Fragments arranged by size:
1426 552 333 197
BsrDI GCAATG_nn'
Cuts at: 0 1678 2508
Size: 1678 830
BssSI C'ACGA_G
Cuts at: 0 581 2508
Size: 581 1927
BstAPI GCAn_nnn'nTGC
Cuts at: 0 385 2259 2508
Size: 385 1874 249
Fragments arranged by size:
1874 385 249
BstEII G'GTnAC_C
Cuts at: 0 690 847 2508
Size: 690 157 1661
Fragments arranged by size:
1661 690 157
BstYI r'GATC_y
Cuts at: 0 1655 2508
Size: 1655 853
Bsu36I CC'TnA_GG
Cuts at: 0 85 2508
Size: 85 2423
BtgI C'CryG_G
Cuts at: 0 471 1155 1475 1724 1906 2508
Size: 471 684 320 249 182 602
Fragments arranged by size:
684 602 471 320 249 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 236 245 2508
Size: 236 9 2263
Fragments arranged by size:
2263 236 9
BtsI GCAGTG_nn'
Cuts at: 0 38 884 2508
Size: 38 846 1624
Fragments arranged by size:
1624 846 38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1751 1786 2508
Size: 1751 35 722
Fragments arranged by size:
1751 722 35
EaeI y'GGCC_r
Cuts at: 0 62 780 1727 1903 1949 2225 2396 2414
Size: 62 718 947 176 46 276 171 18
Cuts at: 2414 2508
Size: 94
Fragments arranged by size:
947 718 276 176 171 94 62 46
18
EagI C'GGCC_G
Cuts at: 0 2225 2508
Size: 2225 283
EarI CTCTTCn'nnn_
Cuts at: 0 822 1541 2010 2508
Size: 822 719 469 498
Fragments arranged by size:
822 719 498 469
EcoICRI GAG'CTC
Cuts at: 0 592 858 2232 2508
Size: 592 266 1374 276
Fragments arranged by size:
1374 592 276 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 313 411 454 1402 1454 1544 1565
Size: 106 207 98 43 948 52 90 21
Cuts at: 1565 2347 2508
Size: 782 161
Fragments arranged by size:
948 782 207 161 106 98 90 52
43 21
EcoO109I rG'GnC_Cy
Cuts at: 0 902 1781 1782 2193 2508
Size: 902 879 1 411 315
Fragments arranged by size:
902 879 411 315 1
EcoRI G'AATT_C
Cuts at: 0 1758 2508
Size: 1758 750
FspI TGC'GCA
Cuts at: 0 700 1702 2508
Size: 700 1002 806
Fragments arranged by size:
1002 806 700
HaeII r_GCGC'y
Cuts at: 0 1820 2068 2314 2468 2508
Size: 1820 248 246 154 40
Fragments arranged by size:
1820 248 246 154 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 1351 1383 2008 2040 2508
Size: 29 32 1290 32 625 32 468
Fragments arranged by size:
1290 625 468 32 32 32 29
HindIII A'AGCT_T
Cuts at: 0 975 2508
Size: 975 1533
KasI G'GCGC_C
Cuts at: 0 1816 2064 2508
Size: 1816 248 444
Fragments arranged by size:
1816 444 248
MscI TGG'CCA
Cuts at: 0 64 782 1729 1905 2508
Size: 64 718 947 176 603
Fragments arranged by size:
947 718 603 176 64
MspA1I CmG'CkG
Cuts at: 0 480 760 874 2070 2091 2103 2202 2343
Size: 480 280 114 1196 21 12 99 141
Cuts at: 2343 2508
Size: 165
Fragments arranged by size:
1196 480 280 165 141 114 99 21
12
NarI GG'CG_CC
Cuts at: 0 1817 2065 2508
Size: 1817 248 443
Fragments arranged by size:
1817 443 248
NcoI C'CATG_G
Cuts at: 0 471 1475 2508
Size: 471 1004 1033
Fragments arranged by size:
1033 1004 471
NheI G'CTAG_C
Cuts at: 0 2470 2508
Size: 2470 38
NsiI A_TGCA'T
Cuts at: 0 5 2508
Size: 5 2503
NspI r_CATG'y
Cuts at: 0 7 2508
Size: 7 2501
PasI CC'CwG_GG
Cuts at: 0 898 2298 2508
Size: 898 1400 210
Fragments arranged by size:
1400 898 210
PflMI CCAn_nnn'nTGG
Cuts at: 0 291 675 687 2130 2508
Size: 291 384 12 1443 378
Fragments arranged by size:
1443 384 378 291 12
PfoI T'CCnGG_A
Cuts at: 0 122 668 2508
Size: 122 546 1840
Fragments arranged by size:
1840 546 122
PmlI CAC'GTG
Cuts at: 0 1796 2508
Size: 1796 712
PpuMI rG'GwC_Cy
Cuts at: 0 902 2193 2508
Size: 902 1291 315
Fragments arranged by size:
1291 902 315
PspOMI G'GGCC_C
Cuts at: 0 1781 2508
Size: 1781 727
PstI C_TGCA'G
Cuts at: 0 33 619 879 1349 2508
Size: 33 586 260 470 1159
Fragments arranged by size:
1159 586 470 260 33
PvuII CAG'CTG
Cuts at: 0 480 760 874 2103 2202 2343 2508
Size: 480 280 114 1229 99 141 165
Fragments arranged by size:
1229 480 280 165 141 114 99
SacI G_AGCT'C
Cuts at: 0 594 860 2234 2508
Size: 594 266 1374 274
Fragments arranged by size:
1374 594 274 266
SanDI GG'GwC_CC
Cuts at: 0 902 2508
Size: 902 1606
SexAI A'CCwGG_T
Cuts at: 0 284 2508
Size: 284 2224
SfcI C'TryA_G
Cuts at: 0 29 615 875 1345 2508
Size: 29 586 260 470 1163
Fragments arranged by size:
1163 586 470 260 29
SfoI GGC'GCC
Cuts at: 0 1818 2066 2508
Size: 1818 248 442
Fragments arranged by size:
1818 442 248
SmaI CCC'GGG
Cuts at: 0 1199 2378 2508
Size: 1199 1179 130
Fragments arranged by size:
1199 1179 130
SphI G_CATG'C
Cuts at: 0 7 2508
Size: 7 2501
StuI AGG'CCT
Cuts at: 0 139 512 1295 2508
Size: 139 373 783 1213
Fragments arranged by size:
1213 783 373 139
StyI C'CwwG_G
Cuts at: 0 471 518 1475 1751 2508
Size: 471 47 957 276 757
Fragments arranged by size:
957 757 471 276 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 1276 2508
Size: 208 1068 1232
Fragments arranged by size:
1232 1068 208
TatI w'GTAC_w
Cuts at: 0 314 2508
Size: 314 2194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1809 2508
Size: 1809 699
XmaI C'CCGG_G
Cuts at: 0 1197 2376 2508
Size: 1197 1179 132
Fragments arranged by size:
1197 1179 132
XmnI GAAnn'nnTTC
Cuts at: 0 448 2508
Size: 448 2060
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BssSI BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI
CspCI EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HindIII KasI MscI MspA1I NarI
NcoI NheI NsiI NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SanDI SexAI SfcI
SfoI SmaI SphI StuI StyI TaqII TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BglII BplI BpuEI BsaBI BsiWI
BspHI BsrFI BsrGI BssHII BstBI BstXI BstZ17I ClaI
DraI DraIII DrdI EciI EcoNI EcoRV FalI FseI
FspAI HincII HpaI KpnI MfeI MluI MmeI MslI
NaeI NdeI NgoMIV NotI NruI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI
XhoI ZraI